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Protein

Transcription initiation factor TFIID subunit 4B

Gene

Taf4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators asond repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDifferentiation, Oogenesis, Spermatogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4B
Alternative name(s):
Transcription initiation factor TFIID 105 kDa subunit
Short name:
TAF(II)105
Short name:
TAFII-105
Short name:
TAFII105
Gene namesi
Name:Taf4b
Synonyms:Taf2c2, Tafii105
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2152345 Taf4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Deficient female mice are infertile due to a granulosa cell defect preventing normal follicle formation. Deficient male are fertile when young, but subsequently exhibit an age-dependent progressive loss of germ cells and misregulation of several genes required for normal spermatogenesis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004309401 – 850Transcription initiation factor TFIID subunit 4BAdd BLAST850

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei584PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiG5E8Z2
PaxDbiG5E8Z2
PeptideAtlasiG5E8Z2
PRIDEiG5E8Z2

PTM databases

iPTMnetiG5E8Z2
PhosphoSitePlusiG5E8Z2

Expressioni

Tissue specificityi

Highly expressed in the testes and ovary, whereas lower levels are detected in most other tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000054321 Expressed in 243 organ(s), highest expression level in cleaving embryo
GenevisibleiG5E8Z2 MM

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain. This heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer. Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers. Interaction with POU2AF1, via its C-terminal activation domain, is required for octamer-dependent transcription.By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-959 General transcription factor complex TFIID, Taf4b variant
IntActiG5E8Z2, 1 interactor
STRINGi10090.ENSMUSP00000126909

Structurei

3D structure databases

ProteinModelPortaliG5E8Z2
SMRiG5E8Z2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 353TAFHPROSITE-ProRule annotationAdd BLAST98
Domaini642 – 691Histone-foldBy similarityAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni504 – 526Required for interaction with P65/RELABy similarityAdd BLAST23
Regioni818 – 850Required for interaction with TAF12By similarityAdd BLAST33

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi509 – 549Nuclear export signalBy similarityAdd BLAST41

Sequence similaritiesi

Belongs to the TAF4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2341 Eukaryota
ENOG410XQS6 LUCA
InParanoidiG5E8Z2
KOiK03129
OMAiPRITRVC
OrthoDBiEOG091G0GOS
TreeFamiTF316520

Family and domain databases

CDDicd08045 TAF4, 1 hit
Gene3Di1.10.20.10, 1 hit
1.20.120.1110, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007900 TAF4
IPR037249 TAFH/NHR1_dom_sf
IPR003894 TAFH_NHR1
PfamiView protein in Pfam
PF05236 TAF4, 1 hit
PF07531 TAFH, 1 hit
SMARTiView protein in SMART
SM00549 TAFH, 1 hit
SUPFAMiSSF158553 SSF158553, 1 hit
SSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS51119 TAFH, 1 hit

Sequencei

Sequence statusi: Complete.

G5E8Z2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPAGLTEPAG AAAPVVSSAS GAVTMAPVAA LPVRVEGTPV ALGPVTKAPV
60 70 80 90 100
SVCVESVAPQ PLPAPVGTLV TKVVPVTALP KLGSPRLPAP QIVTVKTPGT
110 120 130 140 150
TTIQLPANLQ LPPGTVLIKS NSGQLMLVSP QQAVTGAKTT SNITPRPAVP
160 170 180 190 200
ANTQTVKICT VPNSSSQLMK KVTVAPVKNL TQIGTTVATT ASSTSSGQPV
210 220 230 240 250
ALPSSVITVT PAKLVNTVST LKSSSLGVLS TPSNDARLKA ETSVAAQTAL
260 270 280 290 300
PPTVLENVKK CKNFLSMLIK LACSGSQSPE MGQNVKRLVE QLLDAEIEAE
310 320 330 340 350
EFTRKLYIEL KSAPQPHLVP FLKKSVVALR QLLPNSQSFI ENCVKEVSGD
360 370 380 390 400
VVISSCTMTT ATSPVVTSTV SPVLVSGATA PRTLSVQQTL NPLAGPGVAN
410 420 430 440 450
TGVVTLHSVA PAAATGGTTA ATVLLQTSKP LTTSVPNTVA AVSLQPENPV
460 470 480 490 500
VSGAAVTLAI PSATFGEASA TPLCLPSAKP AITSAGTKAD KPAIGTPVQI
510 520 530 540 550
VTQPSTLLPQ AAGIPQTAKV KQLVVQQPSG SSVNHVTSIS HSSPLSTQNC
560 570 580 590 600
GQKTPVNAVM PTSSIIKQIT LPGNKLLSLQ AQRSSIQSNK IKENGPTCFR
610 620 630 640 650
GEDDINDVTF MAEVNLDEEN ACILAAHSDF VGTLIQSCKE EPFLVIGALQ
660 670 680 690 700
KRILDIGKKH DITELNSDAV NLISHATQER LRGLLEKLTT IAQHRMTIYK
710 720 730 740 750
GSENYILSTD TRSQLKFLEK LDQLEKQRKD LEEREMLLKA AKSRSNKEDP
760 770 780 790 800
EQLRLKQKAK ELQQLELAQI QYRDANLTAL AAIGPRKKRP LESGNESFKD
810 820 830 840 850
NPSTSGTSSL TATKPFRPRI TRICLRDLIF CMEQEREMKY SRALYLALLK
Length:850
Mass (Da):89,528
Last modified:October 10, 2018 - v2
Checksum:iD943417A8B31D724
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti799K → Q in BQ952832 (Ref. 3) Curated1
Sequence conflicti808S → F in BQ952832 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102692 Genomic DNA No translation available.
AC125091 Genomic DNA No translation available.
AC133172 Genomic DNA No translation available.
CH466622 Genomic DNA Translation: EDL01590.1
BQ952832 mRNA No translation available.
CCDSiCCDS50227.1
RefSeqiNP_001093919.1, NM_001100449.1
UniGeneiMm.312233

Genome annotation databases

GeneIDi72504
KEGGimmu:72504
UCSCiuc008edl.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC102692 Genomic DNA No translation available.
AC125091 Genomic DNA No translation available.
AC133172 Genomic DNA No translation available.
CH466622 Genomic DNA Translation: EDL01590.1
BQ952832 mRNA No translation available.
CCDSiCCDS50227.1
RefSeqiNP_001093919.1, NM_001100449.1
UniGeneiMm.312233

3D structure databases

ProteinModelPortaliG5E8Z2
SMRiG5E8Z2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-959 General transcription factor complex TFIID, Taf4b variant
IntActiG5E8Z2, 1 interactor
STRINGi10090.ENSMUSP00000126909

PTM databases

iPTMnetiG5E8Z2
PhosphoSitePlusiG5E8Z2

Proteomic databases

MaxQBiG5E8Z2
PaxDbiG5E8Z2
PeptideAtlasiG5E8Z2
PRIDEiG5E8Z2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72504
KEGGimmu:72504
UCSCiuc008edl.2 mouse

Organism-specific databases

CTDi6875
MGIiMGI:2152345 Taf4b

Phylogenomic databases

eggNOGiKOG2341 Eukaryota
ENOG410XQS6 LUCA
InParanoidiG5E8Z2
KOiK03129
OMAiPRITRVC
OrthoDBiEOG091G0GOS
TreeFamiTF316520

Enzyme and pathway databases

ReactomeiR-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Miscellaneous databases

PROiPR:G5E8Z2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000054321 Expressed in 243 organ(s), highest expression level in cleaving embryo
GenevisibleiG5E8Z2 MM

Family and domain databases

CDDicd08045 TAF4, 1 hit
Gene3Di1.10.20.10, 1 hit
1.20.120.1110, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR007900 TAF4
IPR037249 TAFH/NHR1_dom_sf
IPR003894 TAFH_NHR1
PfamiView protein in Pfam
PF05236 TAF4, 1 hit
PF07531 TAFH, 1 hit
SMARTiView protein in SMART
SM00549 TAFH, 1 hit
SUPFAMiSSF158553 SSF158553, 1 hit
SSF47113 SSF47113, 1 hit
PROSITEiView protein in PROSITE
PS51119 TAFH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTAF4B_MOUSE
AccessioniPrimary (citable) accession number: G5E8Z2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: October 10, 2018
Last modified: November 7, 2018
This is version 51 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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