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Protein

Adhesion G protein-coupled receptor F5

Gene

Adgrf5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor that plays a critical role in lung surfactant homeostasis (PubMed:23590306, PubMed:23922714, PubMed:23684610). May play a role in controlling adipocyte function (PubMed:22971422).4 Publications

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-5683826 Surfactant metabolism

Protein family/group databases

MEROPSiP02.032

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor F5
Alternative name(s):
G-protein coupled receptor 116
Gene namesi
Name:Adgrf5
Synonyms:Gpr116
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2182928 Adgrf5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 1019ExtracellularCuratedAdd BLAST998
Transmembranei1020 – 1040HelicalSequence analysisAdd BLAST21
Topological domaini1041 – 1055CytoplasmicCuratedAdd BLAST15
Transmembranei1056 – 1076HelicalSequence analysisAdd BLAST21
Topological domaini1077 – 1092ExtracellularCuratedAdd BLAST16
Transmembranei1093 – 1113HelicalSequence analysisAdd BLAST21
Topological domaini1114 – 1130CytoplasmicCuratedAdd BLAST17
Transmembranei1131 – 1151HelicalSequence analysisAdd BLAST21
Topological domaini1152 – 1175ExtracellularCuratedAdd BLAST24
Transmembranei1176 – 1196HelicalSequence analysisAdd BLAST21
Topological domaini1197 – 1221CytoplasmicCuratedAdd BLAST25
Transmembranei1222 – 1242HelicalSequence analysisAdd BLAST21
Topological domaini1243 – 1250ExtracellularCurated8
Transmembranei1251 – 1271HelicalSequence analysisAdd BLAST21
Topological domaini1272 – 1348CytoplasmicCuratedAdd BLAST77

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice exhibit premature death, decreased body weight and respiratory distress associated with pulmonary alveolar proteinosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000043356422 – 1348Adhesion G protein-coupled receptor F5Add BLAST1327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi291 ↔ 348PROSITE-ProRule annotation
Glycosylationi337N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi389 ↔ 447PROSITE-ProRule annotation
Glycosylationi470N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi490 ↔ 543PROSITE-ProRule annotation
Glycosylationi538N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi665N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei818PhosphoserineBy similarity1
Modified residuei1302PhosphothreonineCombined sources1
Modified residuei1309PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved at multiple sites: one in the GPS domain (S1 site) and the other in the SEA domain (S2 site). The proteolytic cleavage at S1 site generates an extracellular subunit and a seven-transmembrane subunit. The proteolytic cleavage at S2 site generates a fragment that undergoes proteolytic cleavage by the processing enzyme furin.By similarity
Highly glycosylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei51 – 52Cleavage; by furinBy similarity2
Sitei223 – 224CleavageBy similarity2
Sitei992 – 993CleavageBy similarity2

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiG5E8Q8
PaxDbiG5E8Q8
PeptideAtlasiG5E8Q8
PRIDEiG5E8Q8

PTM databases

iPTMnetiG5E8Q8
PhosphoSitePlusiG5E8Q8

Expressioni

Tissue specificityi

Widely expressed and highly expressed in the lung. In the lung predominantly expressed in the alveolar type II epithelial cells.4 Publications

Gene expression databases

BgeeiENSMUSG00000056492 Expressed in 231 organ(s), highest expression level in right lung
ExpressionAtlasiG5E8Q8 baseline and differential
GenevisibleiG5E8Q8 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked. Fragment generates by the processing enzyme furin remains attached to the extracellular N-terminal fragment. Interacts (via N-terminal extracellular domain) with SFTPD (PubMed:23922714).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109229

Structurei

3D structure databases

ProteinModelPortaliG5E8Q8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini163 – 271SEAPROSITE-ProRule annotationAdd BLAST109
Domaini268 – 366Ig-like 1PROSITE-ProRule annotationAdd BLAST99
Domaini367 – 464Ig-like 2PROSITE-ProRule annotationAdd BLAST98
Domaini469 – 559Ig-like 3PROSITE-ProRule annotationAdd BLAST91
Domaini950 – 1004GPSPROSITE-ProRule annotationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi52 – 55Poly-AlaSequence analysis4
Compositional biasi1284 – 1345Ser-richPROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193 Eukaryota
ENOG410XSD2 LUCA
GeneTreeiENSGT00920000148969
InParanoidiG5E8Q8
KOiK08458
OMAiGWDSSGC
OrthoDBiEOG091G00IU
TreeFamiTF316380

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR032471 GAIN_dom_N
IPR017981 GPCR_2-like
IPR008078 GPCR_2_Ig-hepta-like_rcpt
IPR000832 GPCR_2_secretin-like
IPR017983 GPCR_2_secretin-like_CS
IPR000203 GPS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013151 Immunoglobulin
IPR000082 SEA_dom
IPR036364 SEA_dom_sf
PfamiView protein in Pfam
PF00002 7tm_2, 1 hit
PF16489 GAIN, 1 hit
PF01825 GPS, 1 hit
PF07679 I-set, 1 hit
PF00047 ig, 1 hit
PF01390 SEA, 1 hit
PRINTSiPR00249 GPCRSECRETIN
PR01695 IGHEPTARCPTR
SMARTiView protein in SMART
SM00303 GPS, 1 hit
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SUPFAMiSSF48726 SSF48726, 2 hits
SSF82671 SSF82671, 1 hit
PROSITEiView protein in PROSITE
PS00650 G_PROTEIN_RECEP_F2_2, 1 hit
PS50261 G_PROTEIN_RECEP_F2_4, 1 hit
PS50221 GPS, 1 hit
PS50835 IG_LIKE, 3 hits
PS50024 SEA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

G5E8Q8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRSPRTFTFY FLLLVICSSE AALSTPTEPI VQPSILQEHE LAGEELLRPK
60 70 80 90 100
RAAAAGDRVA EEYMVDIEIS FENVSFLESI RAHLNNLSFP IRGTEADILN
110 120 130 140 150
IAMTTVCTPA GNDLLCFCEK GYQWSEERCL HSLTCQDYDS ALPGGYCSCL
160 170 180 190 200
KGLPPQGPFC QLPEAFITLK LKVRLNIGFQ EDLKNTSSAL YRSYKTDLER
210 220 230 240 250
AFRAGYRTLP GFRSVTVTQF TKGSVVVNYV VRVTSAPLPG SIHKANEQVI
260 270 280 290 300
QNLNHTYKMD YNSFQGTPSN ETKFTVIPEF IFEGDNVTLE CETEFVTSNT
310 320 330 340 350
SWYYGEKRSD IQNSDKYSIH TTVINNISLI TRLTIYNFTQ HDAGMYGCNV
360 370 380 390 400
TLDIFEYGTV RKLDVTPIRI LAKEERKVVC DNHPISLNCC SENIANWSSI
410 420 430 440 450
EWKQEGKISI LGNPESDLES SCSTYTLKAD GTQCPSGSSG TTVIYTCEFV
460 470 480 490 500
SAYGARGSKN IAVTFTSVAN LTITRDPISV SEGQSFSITC LSDVSSFDEV
510 520 530 540 550
YWNTSAGIKI HPRFYTMRRY QDGAESVLMV KTSTREWNGT YHCIFRYKNS
560 570 580 590 600
YSIATKDVTV HPLPLVSDIM MDPLEASGLC TSSHQFKCCV EEDAGEEYAV
610 620 630 640 650
TFHVDSSSFP AEREVIGKQA CYTYSLPANL PRCPKDIAVF CHFTNAANSS
660 670 680 690 700
VRSPSMKLKL IPRENVTCQD PIIGIGDPGK VIQKLCQFSG VYGSPGQAIG
710 720 730 740 750
GTVTYKCVGT QWKEESRACI SAPINGLLQV AKALIKSPTQ DQKLPTYLRD
760 770 780 790 800
LSVSAGKEEQ DIRSSPGSLG AIISILDLLS TVPTQVNSEM MRDILATINV
810 820 830 840 850
ILDKSALNSW EKLLQQQSNQ SSQFLHSVER FSQALQLGDS TPPFLAHPNV
860 870 880 890 900
QMKSMVIKRG HPQIYQQQFI FKDSDLWGDV AIDECQLGNL QPDSSIVTVA
910 920 930 940 950
FPTLKAILAQ DVQRKTSSNS LVMTTTVSHN IVKPFRISMT FKNNHRSGGK
960 970 980 990 1000
PQCVFWNFSL ANNTGGWDSS GCSVEDDGRD NRDRVFCKCN HLTSFSILMS
1010 1020 1030 1040 1050
PDSPDPGSLL KILLDIISYI GLGFSIVSLA ACLVVEAMVW KSVTKNRTSY
1060 1070 1080 1090 1100
MRHICIVNIA FCLLIADIWF IVAGAIHDGR YPLNETACVA ATFFIHFFYL
1110 1120 1130 1140 1150
SVFFWMLTLG LMLFYRLIFI LHDASKSTQK AIAFSLGYGC PLIISSITVG
1160 1170 1180 1190 1200
VTQPQEVYMR KNACWLNWED TRALLAFAIP ALIIVVVNVS ITVVVITKIL
1210 1220 1230 1240 1250
RPSIGDKPGK QEKSSLFQIS KSIGVLTPLL GLTWGFGLAT VIQGSNAVFH
1260 1270 1280 1290 1300
IIFTLLNAFQ GLFILLFGCL WDQKVQEALL HKFSLSRWSS QHSKSTSIGS
1310 1320 1330 1340
STPVFSMSSP ISRRFNNLFG KTGTYNVSTP ETTSSSLENS SSAYSLLN
Length:1,348
Mass (Da):149,387
Last modified:December 14, 2011 - v1
Checksum:iBC2CDA96C63B7018
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YDT5A0A286YDT5_MOUSE
Adhesion G protein-coupled receptor...
Adgrf5
1,143Annotation score:
A0A286YD74A0A286YD74_MOUSE
Adhesion G protein-coupled receptor...
Adgrf5
142Annotation score:
A0A286YCD3A0A286YCD3_MOUSE
Adhesion G protein-coupled receptor...
Adgrf5
149Annotation score:
A0A286YE42A0A286YE42_MOUSE
Adhesion G protein-coupled receptor...
Adgrf5
82Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC169504 Genomic DNA No translation available.
CT010585 Genomic DNA No translation available.
CT025700 Genomic DNA No translation available.
CH466559 Genomic DNA Translation: EDL23401.1
CCDSiCCDS37622.1
RefSeqiNP_001074647.1, NM_001081178.1
UniGeneiMm.23478

Genome annotation databases

EnsembliENSMUST00000113599; ENSMUSP00000109229; ENSMUSG00000056492
ENSMUST00000226087; ENSMUSP00000153049; ENSMUSG00000056492
GeneIDi224792
KEGGimmu:224792
UCSCiuc008cpa.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC169504 Genomic DNA No translation available.
CT010585 Genomic DNA No translation available.
CT025700 Genomic DNA No translation available.
CH466559 Genomic DNA Translation: EDL23401.1
CCDSiCCDS37622.1
RefSeqiNP_001074647.1, NM_001081178.1
UniGeneiMm.23478

3D structure databases

ProteinModelPortaliG5E8Q8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109229

Protein family/group databases

MEROPSiP02.032
GPCRDBiSearch...

PTM databases

iPTMnetiG5E8Q8
PhosphoSitePlusiG5E8Q8

Proteomic databases

MaxQBiG5E8Q8
PaxDbiG5E8Q8
PeptideAtlasiG5E8Q8
PRIDEiG5E8Q8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113599; ENSMUSP00000109229; ENSMUSG00000056492
ENSMUST00000226087; ENSMUSP00000153049; ENSMUSG00000056492
GeneIDi224792
KEGGimmu:224792
UCSCiuc008cpa.1 mouse

Organism-specific databases

CTDi221395
MGIiMGI:2182928 Adgrf5

Phylogenomic databases

eggNOGiKOG4193 Eukaryota
ENOG410XSD2 LUCA
GeneTreeiENSGT00920000148969
InParanoidiG5E8Q8
KOiK08458
OMAiGWDSSGC
OrthoDBiEOG091G00IU
TreeFamiTF316380

Enzyme and pathway databases

ReactomeiR-MMU-5683826 Surfactant metabolism

Miscellaneous databases

PROiPR:G5E8Q8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056492 Expressed in 231 organ(s), highest expression level in right lung
ExpressionAtlasiG5E8Q8 baseline and differential
GenevisibleiG5E8Q8 MM

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR032471 GAIN_dom_N
IPR017981 GPCR_2-like
IPR008078 GPCR_2_Ig-hepta-like_rcpt
IPR000832 GPCR_2_secretin-like
IPR017983 GPCR_2_secretin-like_CS
IPR000203 GPS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
IPR013151 Immunoglobulin
IPR000082 SEA_dom
IPR036364 SEA_dom_sf
PfamiView protein in Pfam
PF00002 7tm_2, 1 hit
PF16489 GAIN, 1 hit
PF01825 GPS, 1 hit
PF07679 I-set, 1 hit
PF00047 ig, 1 hit
PF01390 SEA, 1 hit
PRINTSiPR00249 GPCRSECRETIN
PR01695 IGHEPTARCPTR
SMARTiView protein in SMART
SM00303 GPS, 1 hit
SM00409 IG, 2 hits
SM00408 IGc2, 2 hits
SUPFAMiSSF48726 SSF48726, 2 hits
SSF82671 SSF82671, 1 hit
PROSITEiView protein in PROSITE
PS00650 G_PROTEIN_RECEP_F2_2, 1 hit
PS50261 G_PROTEIN_RECEP_F2_4, 1 hit
PS50221 GPS, 1 hit
PS50835 IG_LIKE, 3 hits
PS50024 SEA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAGRF5_MOUSE
AccessioniPrimary (citable) accession number: G5E8Q8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: December 14, 2011
Last modified: October 10, 2018
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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