UniProtKB - G5AY81 (HYAS2_HETGA)
Hyaluronan synthase 2
Has2
Functioni
Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation (By similarity).
This is one of three isoenzymes responsible for cellular hyaluronan synthesis and it is particularly responsible for the synthesis of high molecular mass hyaluronan (By similarity).
By similarityMiscellaneous
Catalytic activityi
- [hyaluronan](n) + UDP-N-acetyl-α-D-glucosamine = H+ + N-acetyl-β-D-glucosaminyl-(1→4)-[hyaluronan](n) + UDPBy similarityEC:2.4.1.212By similarityThis reaction proceeds in the forwardBy similarity direction.
- N-acetyl-β-D-glucosaminyl-(1→4)-[hyaluronan](n) + UDP-α-D-glucuronate = [hyaluronan](n+1) + H+ + UDPBy similarityEC:2.4.1.212By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
: hyaluronan biosynthesis Pathwayi
This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.
GO - Molecular functioni
- hyaluronan synthase activity Source: UniProtKB
GO - Biological processi
- atrioventricular canal development Source: UniProtKB
- bone morphogenesis Source: Ensembl
- cellular response to fluid shear stress Source: Ensembl
- cellular response to interleukin-1 Source: Ensembl
- cellular response to platelet-derived growth factor stimulus Source: Ensembl
- cellular response to tumor necrosis factor Source: Ensembl
- endocardial cushion to mesenchymal transition Source: UniProtKB
- extracellular matrix assembly Source: UniProtKB
- extracellular polysaccharide biosynthetic process Source: UniProtKB
- hyaluronan biosynthetic process Source: UniProtKB
- positive regulation of cell migration Source: Ensembl
- positive regulation of cell population proliferation Source: Ensembl
- positive regulation of monocyte aggregation Source: Ensembl
- positive regulation of urine volume Source: UniProtKB
- renal water absorption Source: UniProtKB
- vasculogenesis Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
UniPathwayi | UPA00341 |
Names & Taxonomyi
Protein namesi | Recommended name: Hyaluronan synthase 2 (EC:2.4.1.212By similarity)Alternative name(s): Hyaluronate synthase 2 Hyaluronic acid synthase 2 |
Gene namesi | Name:Has2 |
Organismi | Heterocephalus glaber (Naked mole rat) |
Taxonomic identifieri | 10181 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricomorpha › Bathyergidae › Heterocephalus |
Proteomesi |
|
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Lysosome
- Lysosome By similarity
Other locations
- Vesicle By similarity
Note: Travels from endoplasmic reticulum (ER), Golgi to plasma membrane and either back to endosomes and lysosomes, or out into extracellular vesicles. Post-translational modifications control HAS2 trafficking.By similarity
Plasma Membrane
- integral component of plasma membrane Source: Ensembl
- plasma membrane raft Source: Ensembl
Other locations
- cytoplasmic vesicle Source: Ensembl
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 32 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 33 – 45 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 46 – 66 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 67 – 374 | CytoplasmicSequence analysisAdd BLAST | 308 | |
Transmembranei | 375 – 395 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 396 – 402 | ExtracellularSequence analysis | 7 | |
Transmembranei | 403 – 423 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 424 – 429 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 430 – 450 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 451 – 475 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 476 – 496 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 497 – 510 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 511 – 531 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 532 – 552 | ExtracellularSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, Golgi apparatus, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424266 | 1 – 552 | Hyaluronan synthase 2Add BLAST | 552 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 110 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 190 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Glycosylationi | 221 | O-linked (GlcNAc) serineBy similarity | 1 | |
Modified residuei | 328 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugationExpressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer; dimerization promotes enzymatic activity. Forms heterodimer with HAS3. Forms heterodimer with HAS1.
By similarityProtein-protein interaction databases
STRINGi | 10181.XP_004838067.1 |
Structurei
3D structure databases
AlphaFoldDBi | G5AY81 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2571, Eukaryota |
InParanoidi | G5AY81 |
OMAi | KSATYVW |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR028371, HAS2 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22913:SF7, PTHR22913:SF7, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MHCERFLCIL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG
60 70 80 90 100
AFLASHLIIQ SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR
110 120 130 140 150
KCLQSVKRLT YPGIKVVMVI DGNSDDDLYM MDIFSEVMGR DKSATYIWKN
160 170 180 190 200
NFHEKGPGET DESHKESSQH VTQLVLSSKS VCIMQKWGGK REVMYTAFRA
210 220 230 240 250
LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG DVQILNKYDS
260 270 280 290 300
WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY
310 320 330 340 350
SQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ
360 370 380 390 400
QTRWSKSYFR EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR
410 420 430 440 450
GKIWNILLFL LTVQLVGLIK SSFASCLRGN IVMVFMSLYS VLYMSSLLPA
460 470 480 490 500
KMFAIATINK AGWGTSGRKT IVVNFIGLIP VSVWFTILLG GVIFTIYKES
510 520 530 540 550
KKPFSESKQT VLIVGTLLYA CYWVMLLTLY VVLINKCGRR KKGQQYDMVL
DV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JH167500 Genomic DNA Translation: EHB01992.1 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight an unexpected turn of events - Issue 155 of December 2013 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | JH167500 Genomic DNA Translation: EHB01992.1 |
3D structure databases
AlphaFoldDBi | G5AY81 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10181.XP_004838067.1 |
Phylogenomic databases
eggNOGi | KOG2571, Eukaryota |
InParanoidi | G5AY81 |
OMAi | KSATYVW |
Enzyme and pathway databases
UniPathwayi | UPA00341 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR028371, HAS2 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR22913:SF7, PTHR22913:SF7, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HYAS2_HETGA | |
Accessioni | G5AY81Primary (citable) accession number: G5AY81 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2013 |
Last sequence update: | December 14, 2011 | |
Last modified: | May 25, 2022 | |
This is version 43 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries