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UniProtKB - G4NAZ1 (CE12A_MAGO7)
Protein
Endoglucanase cel12A
Gene
cel12A
Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Functioni
Endoglucanase that functions in part to hydrolyze 1,3-1,4-beta-glucan during infection and spore formation. Shows preferential hydrolysis of barley beta-glucan and lichenan.
1 PublicationMiscellaneous
The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the disaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.Curated
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.1 Publication EC:3.2.1.4
Kineticsi
- KM=0.9 mg/ml for barley beta-glucan (when expressed in M.oryzae)1 Publication
- KM=2.87 mg/ml for barley beta-glucan (when expressed in B.choshinensis)1 Publication
pH dependencei
Optimum pH is 6.0-7.5.1 Publication
Temperature dependencei
Optimum temperature is 30-50 degrees Celsius.1 Publication
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Protein family/group databases
CAZyi | GH12, Glycoside Hydrolase Family 12 |
CLAEi | EGL12A_MAGOR |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase cel12A1 Publication (EC:3.2.1.41 Publication)Alternative name(s): Cellulase cel12ACurated Endo-1,4-beta-glucanase cel12ACurated |
Gene namesi | Name:cel12A1 Publication ORF Names:MGG_00677 |
Organismi | Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) |
Taxonomic identifieri | 242507 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Pyriculariaceae › Pyricularia › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | FungiDB:MGG_00677 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
ChainiPRO_0000432716 | 17 – 257 | Endoglucanase cel12ASequence analysisAdd BLAST | 241 |
Expressioni
Inductioni
Levels of expression are constitutively high during infection and spore formation.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 12 (cellulase H) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502SH4Y, Eukaryota |
HOGENOMi | CLU_051064_0_1_1 |
InParanoidi | G4NAZ1 |
OMAi | NRWGQGS |
OrthoDBi | 1122671at2759 |
Family and domain databases
Gene3Di | 2.60.120.180, 1 hit |
InterProi | View protein in InterPro IPR013320, ConA-like_dom_sf IPR013319, GH11/12 IPR002594, GH12 |
PANTHERi | PTHR34002, PTHR34002, 1 hit |
Pfami | View protein in Pfam PF01670, Glyco_hydro_12, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
G4NAZ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTSAALLFL VSLASAAPMT PSPAAEAAAV LEGRQIQTLC DQYGYWSGNG
60 70 80 90 100
YYVNNNMWGK DSGSGSQCTY IDSSSANHVS WRSTWQWSGG QNNVKSYPYS
110 120 130 140 150
GRTIQRGRTI ASINSMPSVV QWNYNNGNIR ANVAYDLFTS TDPNRDNTHG
160 170 180 190 200
DYELMVWLGR YGDVQPIGSQ IGNANVGGRN WQLWSGLNNG MRVYSFVTTQ
210 220 230 240 250
GAITYFNADI KQFFTYLQQS QGFPASQQYL TTFQFGTEPF TGGQTTFQVN
SFGANVQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM001235 Genomic DNA Translation: EHA48753.1 |
RefSeqi | XP_003718337.1, XM_003718289.1 |
Genome annotation databases
EnsemblFungii | MGG_00677T0; MGG_00677T0; MGG_00677 |
GeneIDi | 2674686 |
KEGGi | mgr:MGG_00677 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CM001235 Genomic DNA Translation: EHA48753.1 |
RefSeqi | XP_003718337.1, XM_003718289.1 |
3D structure databases
SMRi | G4NAZ1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 318829.MGG_00677T0 |
Protein family/group databases
CAZyi | GH12, Glycoside Hydrolase Family 12 |
CLAEi | EGL12A_MAGOR |
Genome annotation databases
EnsemblFungii | MGG_00677T0; MGG_00677T0; MGG_00677 |
GeneIDi | 2674686 |
KEGGi | mgr:MGG_00677 |
Organism-specific databases
VEuPathDBi | FungiDB:MGG_00677 |
Phylogenomic databases
eggNOGi | ENOG502SH4Y, Eukaryota |
HOGENOMi | CLU_051064_0_1_1 |
InParanoidi | G4NAZ1 |
OMAi | NRWGQGS |
OrthoDBi | 1122671at2759 |
Family and domain databases
Gene3Di | 2.60.120.180, 1 hit |
InterProi | View protein in InterPro IPR013320, ConA-like_dom_sf IPR013319, GH11/12 IPR002594, GH12 |
PANTHERi | PTHR34002, PTHR34002, 1 hit |
Pfami | View protein in Pfam PF01670, Glyco_hydro_12, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CE12A_MAGO7 | |
Accessioni | G4NAZ1Primary (citable) accession number: G4NAZ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 2015 |
Last sequence update: | December 14, 2011 | |
Last modified: | September 29, 2021 | |
This is version 43 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families