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Protein

Ceramide glucosyltransferase

Gene

MGG_10668

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the final step in the biosynthesis of the membrane lipid glucosylceramide (GluCer), the transfer of glucose to ceramide. Glucosylceramides play important roles in growth, differentiation and pathogenicity.1 Publication

Catalytic activityi

UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.1 Publication

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei285Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayi
UPA00222

Protein family/group databases

CAZyiGT21 Glycosyltransferase Family 21

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide glucosyltransferaseBy similarity (EC:2.4.1.801 Publication)
Alternative name(s):
GLCT-1
Glucosylceramide synthase1 Publication
Short name:
GCS
UDP-glucose ceramide glucosyltransferase
UDP-glucose:N-acylsphingosine D-glucosyltransferase
Gene namesi
ORF Names:MGG_10668
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:MGG_10668

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalCurated6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 337CytoplasmicBy similarityAdd BLAST310
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359 – 380LumenalCuratedAdd BLAST22
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 428CytoplasmicCuratedAdd BLAST27
Transmembranei429 – 449HelicalSequence analysisAdd BLAST21
Topological domaini450 – 494LumenalCuratedAdd BLAST45

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004348071 – 494Ceramide glucosyltransferaseAdd BLAST494

Interactioni

Protein-protein interaction databases

STRINGi318829.MGG_10668T0

Structurei

3D structure databases

ProteinModelPortaliG4MS28
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95D1Curated1
Motifi160D2Curated1
Motifi285D3Curated1
Motifi326 – 330(Q/R)XXRWCurated5

Domaini

The D1, D2, D3, (Q/R)XXRW motif is a critical part of the GCS active site, involved in catalysis and UDP-sugar binding.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2547 Eukaryota
COG1215 LUCA
InParanoidiG4MS28
KOiK00720
OrthoDBiEOG092C2GCN

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR025993 Ceramide_glucosylTrfase
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR12726 PTHR12726, 1 hit
PfamiView protein in Pfam
PF13506 Glyco_transf_21, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

G4MS28-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLLMDGLAY AGAIWSLIVF CVQAIGLYQL FRSYSRPPPP PVSPSLTSED
60 70 80 90 100
VPHVTVIRPV KGLEPRLYEC LISTLQQSYP RDKLSVHLCI SSKEDPAYPV
110 120 130 140 150
LKKVVVEYSA THDVRLFVET EDPLLYGTTG DTRNLGPNPK IRNISHAYRE
160 170 180 190 200
AKGDIIWIID CNIWVSKGTA GRMVDKLCGF PAGSRPYKFV HQLPLSVDVT
210 220 230 240 250
PPQDSGVLRT GGGRLDEMFM ATTHGKFYSA INTVGVAPCI CGKSNMFRKS
260 270 280 290 300
HLDRLTDPAH NPILPKETAT RPRGIDYFSA YICEDHLIGD LLWRSQVPGH
310 320 330 340 350
GNHGLVFGDL ALQPMMNNSV GSYIARRVRW LRVRKWTVLL ATLVEPGVES
360 370 380 390 400
MVCCMAFAHA LTTTPWCPNP ADWPIPHTWT ALWSIWLAAI AVWATLDYVV
410 420 430 440 450
YHFLHSCRSI EKDADSPDFA QGNELMKRPF GAWILAWIGR EILALPIWTR
460 470 480 490
AVLLGTTVTW RGTKFKVRPD QSVVDIPNAG AKSNGIGSTN RKVR
Length:494
Mass (Da):55,081
Last modified:December 14, 2011 - v1
Checksum:iE60EA8DA11083E41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF364402 mRNA Translation: AAK73019.1
CM001231 Genomic DNA Translation: EHA57494.1
RefSeqiXP_003710106.1, XM_003710058.1

Genome annotation databases

EnsemblFungiiMGG_10668T0; MGG_10668T0; MGG_10668
GeneIDi2682281
KEGGimgr:MGG_10668

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF364402 mRNA Translation: AAK73019.1
CM001231 Genomic DNA Translation: EHA57494.1
RefSeqiXP_003710106.1, XM_003710058.1

3D structure databases

ProteinModelPortaliG4MS28
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318829.MGG_10668T0

Protein family/group databases

CAZyiGT21 Glycosyltransferase Family 21

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_10668T0; MGG_10668T0; MGG_10668
GeneIDi2682281
KEGGimgr:MGG_10668

Organism-specific databases

EuPathDBiFungiDB:MGG_10668

Phylogenomic databases

eggNOGiKOG2547 Eukaryota
COG1215 LUCA
InParanoidiG4MS28
KOiK00720
OrthoDBiEOG092C2GCN

Enzyme and pathway databases

UniPathwayi
UPA00222

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR025993 Ceramide_glucosylTrfase
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR12726 PTHR12726, 1 hit
PfamiView protein in Pfam
PF13506 Glyco_transf_21, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEGT_MAGO7
AccessioniPrimary (citable) accession number: G4MS28
Secondary accession number(s): Q96V38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 9, 2015
Last sequence update: December 14, 2011
Last modified: October 10, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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