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Protein

Thymidylate synthase

Gene

thyA

Organism
Bacillus subtilis subsp. subtilis str. SC-8
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24dUMPUniRule annotation1
Binding sitei545,10-methylenetetrahydrofolateUniRule annotation1
Active sitei149PROSITE-ProRule annotation1
Active sitei149NucleophileUniRule annotation1
Binding sitei1725,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei180dUMPUniRule annotation1
Binding sitei2665,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 130dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi169 – 172dUMPUniRule annotation4
Nucleotide bindingi210 – 212dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
ORF Names:BSSC8_20880Imported
OrganismiBacillus subtilis subsp. subtilis str. SC-8Imported
Taxonomic identifieri1089443 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000004759 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 267Thymidylat_syntInterPro annotationAdd BLAST263

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

G4EUQ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMKQYKDF CRHVLEHGEK KGDRTGTGTI STFGYQMRFN LREGFPMLTT
60 70 80 90 100
KKLHFKSIAH ELLWFLKGDT NVRYLQENGV RIWNEWADEN GELGPVYGSQ
110 120 130 140 150
WRSWRGADGE TIDQISRLIE DIKTNPNSRR LIVSAWNVGE IDKMALPPCH
160 170 180 190 200
CLFQFYVSDG KLSCQLYQRS ADVFLGVPFN IASYALLTMI IAHVTGLEPG
210 220 230 240 250
EFIHTFGDVH IYQNHIEQVN LQLERDVRPL PQLRFARKVD SIFNFAFEDF
260
IIEDYDPHPH IKGAVSV
Length:267
Mass (Da):30,925
Last modified:December 14, 2011 - v1
Checksum:i21FAE10D67CFD2FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AGFW01000003 Genomic DNA Translation: EHA31645.1

Genome annotation databases

EnsemblBacteriaiEHA31645; EHA31645; BSSC8_20880
PATRICifig|1089443.3.peg.2106

Similar proteinsi

Entry informationi

Entry nameiG4EUQ8_BACIU
AccessioniPrimary (citable) accession number: G4EUQ8
Entry historyiIntegrated into UniProtKB/TrEMBL: December 14, 2011
Last sequence update: December 14, 2011
Last modified: February 28, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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