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Protein

SprT-like domain-containing protein Spartan

Gene

Sprtn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (TLS). Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Acts as a regulator of TLS by recruiting VCP/p97 to sites of DNA damage, possibly leading to extraction of DNA polymerase eta (POLH) by VCP/p97 to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri462 – 485UBZ-typeAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110320 Translesion Synthesis by POLH

Names & Taxonomyi

Protein namesi
Recommended name:
SprT-like domain-containing protein Spartan
Alternative name(s):
Protein with SprT-like domain at the N terminus
Short name:
Spartan
Gene namesi
Name:Sprtn
Synonyms:Gm505
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2685351 Sprtn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004194841 – 497SprT-like domain-containing protein SpartanAdd BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei269PhosphoserineBy similarity1
Cross-linki304Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki342Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki362Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki432Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiG3X912
MaxQBiG3X912
PaxDbiG3X912
PRIDEiG3X912

PTM databases

iPTMnetiG3X912
PhosphoSitePlusiG3X912

Expressioni

Gene expression databases

BgeeiENSMUSG00000031986 Expressed in 21 organ(s), highest expression level in testis

Interactioni

Subunit structurei

Interacts with PCNA (when ubiquitinated). Interacts with RAD18. Interacts (via its SHP-box) with VCP/p97. Interacts with KCTD13 and POLD3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi232674, 1 interactor
STRINGi10090.ENSMUSP00000034467

Structurei

3D structure databases

ProteinModelPortaliG3X912
SMRiG3X912
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 213SprT-likeAdd BLAST168

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi254 – 262SHP-box9
Motifi326 – 333PIP-box8

Domaini

The PIP-box mediates the interaction with PCNA, while the UBZ-type zinc finger mediates binding to 'Lys-48'- and 'Lys-63'-linked polyubiquitin.By similarity

Sequence similaritiesi

Belongs to the Spartan family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri462 – 485UBZ-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3931 Eukaryota
ENOG410XPXU LUCA
GeneTreeiENSGT00390000003585
HOGENOMiHOG000031496
HOVERGENiHBG101206
InParanoidiG3X912
OMAiNEHLDWC
OrthoDBiEOG091G02ZI
PhylomeDBiG3X912
TreeFamiTF314762

Family and domain databases

InterProiView protein in InterPro
IPR006640 SprT-like_domain
IPR006642 Znf_Rad18_put
PfamiView protein in Pfam
PF10263 SprT-like, 1 hit
SMARTiView protein in SMART
SM00731 SprT, 1 hit
SM00734 ZnF_Rad18, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

G3X912-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEDLVVALR LQEEWDVQMA RRAAAAREPV SLVDASWELV DPTPDLQALF
60 70 80 90 100
LQFNDRFFWG QLEAVEVKWS VRMTLCAGIC TYEGRGGMCS IRLSEPLLKL
110 120 130 140 150
RPRKDLVETL LHEMIHAYLF VTNNDKDREG HGPEFCKHMH RINQLTGANI
160 170 180 190 200
TVYHTFHDEV DEYRRHWWRC NGPCQHRQPY YGYVKRATNR APSVHDYWWA
210 220 230 240 250
DHQKTCGGTY IKIKEPENYS KKGRGKTKAD KQPASAVENK DKLCRGEAQL
260 270 280 290 300
LIPFSGKGYV LGDASTCPSA GKLNTSYMVN EAKGLSSQDH SVSGLRLNSN
310 320 330 340 350
AEVKCEQNCL PKKPHLVSPL PTASHQSVLS SYFPRVSVAN QKAFRNVNGS
360 370 380 390 400
PVKNGTTGDG TKRPASGGSQ RKVPPSRASL RNTSKVTAPA SATVTSAAGT
410 420 430 440 450
SATISREESG SEDQFLNKRP RLEDRTALDT IKEQTQSGGD LRSSSQPTAA
460 470 480 490
SAPQSLSSQR RLVNCPVCQG VVVESQINEH LDRCLEGNKT NLRPRRV
Length:497
Mass (Da):55,317
Last modified:November 16, 2011 - v1
Checksum:i92B47BFBFD4616C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti413D → N in AAI58089 (PubMed:15489334).Curated1
Sequence conflicti442R → Q in AAI58089 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC139158 Genomic DNA No translation available.
CH466525 Genomic DNA Translation: EDL11789.1
BC158088 mRNA Translation: AAI58089.1
CCDSiCCDS52705.1
RefSeqiNP_001104611.1, NM_001111141.1
UniGeneiMm.482915

Genome annotation databases

EnsembliENSMUST00000034467; ENSMUSP00000034467; ENSMUSG00000031986
GeneIDi244666
KEGGimmu:244666
UCSCiuc009nxx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC139158 Genomic DNA No translation available.
CH466525 Genomic DNA Translation: EDL11789.1
BC158088 mRNA Translation: AAI58089.1
CCDSiCCDS52705.1
RefSeqiNP_001104611.1, NM_001111141.1
UniGeneiMm.482915

3D structure databases

ProteinModelPortaliG3X912
SMRiG3X912
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232674, 1 interactor
STRINGi10090.ENSMUSP00000034467

PTM databases

iPTMnetiG3X912
PhosphoSitePlusiG3X912

Proteomic databases

EPDiG3X912
MaxQBiG3X912
PaxDbiG3X912
PRIDEiG3X912

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034467; ENSMUSP00000034467; ENSMUSG00000031986
GeneIDi244666
KEGGimmu:244666
UCSCiuc009nxx.2 mouse

Organism-specific databases

CTDi83932
MGIiMGI:2685351 Sprtn

Phylogenomic databases

eggNOGiKOG3931 Eukaryota
ENOG410XPXU LUCA
GeneTreeiENSGT00390000003585
HOGENOMiHOG000031496
HOVERGENiHBG101206
InParanoidiG3X912
OMAiNEHLDWC
OrthoDBiEOG091G02ZI
PhylomeDBiG3X912
TreeFamiTF314762

Enzyme and pathway databases

ReactomeiR-MMU-110320 Translesion Synthesis by POLH

Miscellaneous databases

PROiPR:G3X912
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031986 Expressed in 21 organ(s), highest expression level in testis

Family and domain databases

InterProiView protein in InterPro
IPR006640 SprT-like_domain
IPR006642 Znf_Rad18_put
PfamiView protein in Pfam
PF10263 SprT-like, 1 hit
SMARTiView protein in SMART
SM00731 SprT, 1 hit
SM00734 ZnF_Rad18, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPRTN_MOUSE
AccessioniPrimary (citable) accession number: G3X912
Secondary accession number(s): B2RY42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: November 16, 2011
Last modified: November 7, 2018
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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