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Protein

Cyclic AMP-dependent transcription factor ATF-6 alpha

Gene

Atf6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation, Unfolded protein response

Enzyme and pathway databases

ReactomeiR-RNO-381033 ATF6 (ATF6-alpha) activates chaperones

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-dependent transcription factor ATF-6 alpha
Short name:
cAMP-dependent transcription factor ATF-6 alpha
Alternative name(s):
Activating transcription factor 6 alpha
Short name:
ATF6-alpha
Cleaved into the following chain:
Gene namesi
Name:Atf6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1305471 Atf6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei378 – 398Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini399 – 656LumenalSequence analysisAdd BLAST258

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004243381 – 656Cyclic AMP-dependent transcription factor ATF-6 alphaAdd BLAST656
ChainiPRO_00004243391 – ?Processed cyclic AMP-dependent transcription factor ATF-6 alphaBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki75Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Glycosylationi459N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi570N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi629N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (By similarity).By similarity
N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR) (By similarity).By similarity
Phosphorylated in vitro by MAPK14/P38MAPK.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei406 – 407Cleavage; by PS1By similarity2

Keywords - PTMi

Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiG3V909

Expressioni

Gene expression databases

BgeeiENSRNOG00000024632 Expressed in 9 organ(s), highest expression level in liver
GenevisibleiG3V909 RN

Interactioni

Subunit structurei

Homodimer and heterodimer with ATF6-beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). Interacts also with the transcription factors GTF2I, YY1 and SRF. Interacts (via lumenal domain) with THBS1. Interacts with THBS4 (via EGF-like 3; calcium-binding domain) which facilitates its processing, activation and nuclear translocation. Interacts with XBP1; the interaction occurs in a ER stress-dependent manner (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000032766

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini293 – 356bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 137Transcription activationBy similarityAdd BLAST137
Regioni295 – 326Basic motifPROSITE-ProRule annotationAdd BLAST32
Regioni335 – 342Leucine-zipperPROSITE-ProRule annotation8
Regioni455 – 575Interaction with THBS4By similarityAdd BLAST121

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 131Ser-richAdd BLAST71
Compositional biasi450 – 453Poly-Pro4

Domaini

The basic domain functions as a nuclear localization signal.By similarity
The basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE.By similarity

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4343 Eukaryota
ENOG410ZAAP LUCA
GeneTreeiENSGT00530000063762
HOGENOMiHOG000253938
HOVERGENiHBG108357
InParanoidiG3V909
KOiK09054
OMAiYRYDHSV
OrthoDBiEOG091G0JCT
PhylomeDBiG3V909
TreeFamiTF316079

Family and domain databases

InterProiView protein in InterPro
IPR029801 ATF6A
IPR004827 bZIP
PANTHERiPTHR22952:SF116 PTHR22952:SF116, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

G3V909-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESPFSPVLP HGPGEDWEST LFAELGYFTD TDDVQFDAAH ETYENNFDHL
60 70 80 90 100
NFDLDLMPWE SDIWSPSSHF CSDIKAEPQP LSPASSSCSV SSPRSTDSCS
110 120 130 140 150
STQHVPEELD LLSSSQSPLS LYGDSCHSPS SAEPLKEEKP VTGPGNKTEH
160 170 180 190 200
GLTPKKKIQM SSKPSVQPKP LLLPAAPKTP ANASVPAKTI IIQTLPALMP
210 220 230 240 250
LAKQQSIISI QPAPTKGQTV LLSQPAVVQL QTPGVLPSAQ PVLAVTGGAT
260 270 280 290 300
QLPNHVVNVV PAPVVNSPVN GKLCVTKPVL QSSTRSTGSD IAVLRRQQRM
310 320 330 340 350
IKNRESACQS RKKKKEYMLG LEARLKAALS ENEQLKKENG SLKRQLDQVV
360 370 380 390 400
SENQRLKVPS PKRRAVCVMI VLAFIMLNYG PMSMLEQDSR RVKPSVSPAN
410 420 430 440 450
QRRHLLEFSA KEVKDTSDGD NQKNSYRYDH SVSNDKALMV LSEEPLLYIP
460 470 480 490 500
PPPCQPLINT TESLRLNHEL RGWVHRHEVE RTKSRRMTNS QQKTRILQGA
510 520 530 540 550
LEQGSNSQLM AVQYTETTSI SRNSGSELQV YYASPGSYQG FFDAIRRRGD
560 570 580 590 600
TFYVVSFRRD HLLLPATTHN KTTRPKMSIV LPAININDNV INGQDYEVMM
610 620 630 640 650
QIDCQVMDTR ILHIKSSSVP PYLRDHQRNQ TSTFFGSPPT ATETTHVVST

LPESVQ
Length:656
Mass (Da):72,665
Last modified:November 16, 2011 - v1
Checksum:i2FB1DE2B3EEE6E04
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti580V → G in AAI68890 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06076112 Genomic DNA No translation available.
AABR06076113 Genomic DNA No translation available.
AABR06076114 Genomic DNA No translation available.
AABR06076115 Genomic DNA No translation available.
AABR06076116 Genomic DNA No translation available.
CH473958 Genomic DNA Translation: EDM09246.1
BC168890 mRNA Translation: AAI68890.1
RefSeqiNP_001100666.1, NM_001107196.1
UniGeneiRn.15235

Genome annotation databases

EnsembliENSRNOT00000029202; ENSRNOP00000032766; ENSRNOG00000024632
GeneIDi304962
KEGGirno:304962

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06076112 Genomic DNA No translation available.
AABR06076113 Genomic DNA No translation available.
AABR06076114 Genomic DNA No translation available.
AABR06076115 Genomic DNA No translation available.
AABR06076116 Genomic DNA No translation available.
CH473958 Genomic DNA Translation: EDM09246.1
BC168890 mRNA Translation: AAI68890.1
RefSeqiNP_001100666.1, NM_001107196.1
UniGeneiRn.15235

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000032766

Proteomic databases

PaxDbiG3V909

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029202; ENSRNOP00000032766; ENSRNOG00000024632
GeneIDi304962
KEGGirno:304962

Organism-specific databases

CTDi22926
RGDi1305471 Atf6

Phylogenomic databases

eggNOGiKOG4343 Eukaryota
ENOG410ZAAP LUCA
GeneTreeiENSGT00530000063762
HOGENOMiHOG000253938
HOVERGENiHBG108357
InParanoidiG3V909
KOiK09054
OMAiYRYDHSV
OrthoDBiEOG091G0JCT
PhylomeDBiG3V909
TreeFamiTF316079

Enzyme and pathway databases

ReactomeiR-RNO-381033 ATF6 (ATF6-alpha) activates chaperones

Miscellaneous databases

PROiPR:G3V909

Gene expression databases

BgeeiENSRNOG00000024632 Expressed in 9 organ(s), highest expression level in liver
GenevisibleiG3V909 RN

Family and domain databases

InterProiView protein in InterPro
IPR029801 ATF6A
IPR004827 bZIP
PANTHERiPTHR22952:SF116 PTHR22952:SF116, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATF6A_RAT
AccessioniPrimary (citable) accession number: G3V909
Secondary accession number(s): B5DF18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: November 16, 2011
Last modified: September 12, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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