Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium channel subfamily K member 4

Gene

Kcnk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Voltage-insensitive potassium channel (PubMed:11374070, PubMed:15677687). Channel opening is triggered by mechanical forces that deform the membrane, and by raising the intracellular pH to basic levels (PubMed:11374070, PubMed:15677687). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (PubMed:15677687). Plays a role in the perception of pain caused by heat (By similarity). Plays a role in the sensory perception of pain caused by pressure (By similarity).By similarity2 Publications

Activity regulationi

Activated by arachidonic acid.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-RNO-1299503 TWIK related potassium channel (TREK)
R-RNO-5576886 Phase 4 - resting membrane potential

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein
Short name:
TRAAK
Gene namesi
Name:Kcnk4Imported
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621449 Kcnk4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicBy similarity3
Transmembranei4 – 24HelicalBy similarityAdd BLAST21
Topological domaini25 – 88ExtracellularBy similarityAdd BLAST64
Intramembranei89 – 103Helical; Name=Pore helix 1By similarityAdd BLAST15
Intramembranei104 – 110By similarity7
Topological domaini111 – 118ExtracellularBy similarity8
Transmembranei119 – 151HelicalBy similarityAdd BLAST33
Topological domaini152 – 173CytoplasmicBy similarityAdd BLAST22
Transmembranei174 – 195HelicalBy similarityAdd BLAST22
Topological domaini196 – 200ExtracellularBy similarity5
Intramembranei201 – 214Helical; Name=Pore helix 2By similarityAdd BLAST14
Intramembranei215 – 220By similarity6
Topological domaini221 – 234ExtracellularBy similarityAdd BLAST14
Transmembranei235 – 261HelicalBy similarityAdd BLAST27
Topological domaini262 – 397CytoplasmicBy similarityAdd BLAST136

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004325911 – 397Potassium channel subfamily K member 4Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52InterchainBy similarity
Glycosylationi81N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiG3V8V5

Expressioni

Tissue specificityi

Detected in brain, and at much lower levels in liver, skeletal muscle and testis.1 Publication

Gene expression databases

BgeeiENSRNOG00000021140 Expressed in 8 organ(s), highest expression level in brain
GenevisibleiG3V8V5 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028704

Structurei

3D structure databases

ProteinModelPortaliG3V8V5
SMRiG3V8V5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 109Selectivity filter 1By similarity6
Regioni213 – 218Selectivity filter 2By similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi293 – 336Pro-richPROSITE-ProRule annotationAdd BLAST44

Domaini

Channel opening is brought about by a conformation change that involves buckling of the second transmembrane helix and affects the position and orientation of the fourth transmembrane helix.By similarity

Sequence similaritiesi

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418 Eukaryota
COG1226 LUCA
GeneTreeiENSGT00760000118858
HOVERGENiHBG052234
KOiK04915
OMAiLDYPSEN
OrthoDBiEOG091G0E3R
PhylomeDBiG3V8V5
TreeFamiTF313947

Family and domain databases

InterProiView protein in InterPro
IPR003280 2pore_dom_K_chnl
IPR008074 2pore_dom_K_chnl_TRAAK
IPR013099 K_chnl_dom
PfamiView protein in Pfam
PF07885 Ion_trans_2, 2 hits
PRINTSiPR01333 2POREKCHANEL
PR01691 TRAAKCHANNEL

Sequencei

Sequence statusi: Complete.

G3V8V5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRSTTLLALL ALVLLYLVSG ALVFQALEQP HEQQVQKDLE DGRDQFLKDH
60 70 80 90 100
PCVSQKNLEG FIKLVAEALG GGANPETSWT NSSNHSSAWN LGSAFFFSGT
110 120 130 140 150
IITTIGYGNI ALHTDAGRLF CIFYALVGIP LFGMLLAGVG DRLGSSLRRG
160 170 180 190 200
IGHIEAVFLK WHVPPGLVRM LSAVLFLLIG CLLFVLTPTF VFSYMESWSK
210 220 230 240 250
LEAIYFVIVT LTTVGFGDYV PGDGTGQNSP AYQPLVWFWI LFGLAYFASV
260 270 280 290 300
LTTIGNWLRA VSRRTRAEMG GLTAQAASWT GTVTARVTQR TGPSAPPPEK
310 320 330 340 350
EQPLLPSSLP APPAVAEPAH RPGSPAPAEK VETPPPTASA LDYPSENLAF
360 370 380 390
IDESSDTQSE RGCALPRAPR GRRRPNPTKK PSRPRGPGRL RDKAVPV
Length:397
Mass (Da):42,920
Last modified:November 16, 2011 - v1
Checksum:i5369F0841EE72831
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25Q → Y in AAK60504 (PubMed:11374070).Curated1
Sequence conflicti196E → K in AAK60504 (PubMed:11374070).Curated1
Sequence conflicti202E → K in AAK60504 (PubMed:11374070).Curated1
Sequence conflicti251L → F in AAK60504 (PubMed:11374070).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302842 mRNA Translation: AAK60504.2
AABR06009566 Genomic DNA No translation available.
CH473953 Genomic DNA Translation: EDM12628.1
RefSeqiNP_446256.2, NM_053804.2
XP_008758276.1, XM_008760054.2
XP_008758277.1, XM_008760055.2
XP_008758278.1, XM_008760056.2
UniGeneiRn.46453

Genome annotation databases

EnsembliENSRNOT00000028704; ENSRNOP00000028704; ENSRNOG00000021140
GeneIDi116489
KEGGirno:116489

Similar proteinsi

Entry informationi

Entry nameiKCNK4_RAT
AccessioniPrimary (citable) accession number: G3V8V5
Secondary accession number(s): Q924I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 2015
Last sequence update: November 16, 2011
Last modified: September 12, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again