UniProtKB - G1KNY7 (G1KNY7_ANOCA)
Protein
AP-2 complex subunit alpha
Gene
ap2a1
Organism
Anolis carolinensis (Green anole) (American chameleon)
Status
Functioni
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 75 | Phosphatidylinositol 3,4,5-trisphosphateUniRule annotation | 1 | |
Binding sitei | 85 | Phosphatidylinositol 3,4,5-trisphosphateUniRule annotation | 1 |
GO - Molecular functioni
- cargo adaptor activity Source: GO_Central
- clathrin adaptor activity Source: GO_Central
- low-density lipoprotein particle receptor binding Source: Ensembl
- protein kinase binding Source: Ensembl
GO - Biological processi
- clathrin-dependent endocytosis Source: GO_Central
- intracellular protein transport Source: UniProtKB-UniRule
- negative regulation of hyaluronan biosynthetic process Source: Ensembl
- receptor-mediated endocytosis Source: GO_Central
Keywordsi
Biological process | EndocytosisUniRule annotationARBA annotation, Protein transportUniRule annotationARBA annotation, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: AP-2 complex subunit alphaUniRule annotation |
Gene namesi | Name:ap2a1Imported |
Organismi | Anolis carolinensis (Green anole) (American chameleon)Imported |
Taxonomic identifieri | 28377 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Iguania › Dactyloidae › Anolis |
Proteomesi |
|
Subcellular locationi
Other locations
- coated pit UniRule annotation
Plasma Membrane
- AP-2 adaptor complex Source: GO_Central
Other locations
- filopodium tip Source: Ensembl
Keywords - Cellular componenti
Coated pitUniRule annotationARBA annotation, MembraneExpressioni
Gene expression databases
Bgeei | ENSACAG00000013405, Expressed in hemipenis and 14 other tissues |
Interactioni
Subunit structurei
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).
UniRule annotationGO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- low-density lipoprotein particle receptor binding Source: Ensembl
- protein kinase binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 28377.ENSACAP00000013323 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 735 – 848 | Alpha_adaptinC2InterPro annotationAdd BLAST | 114 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 17 – 37 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 43 – 44 | Phosphatidylinositol 3,4,5-trisphosphate bindingUniRule annotation | 2 | |
Regioni | 89 – 93 | Phosphatidylinositol 3,4,5-trisphosphate bindingUniRule annotation | 5 | |
Regioni | 649 – 693 | DisorderedSequence analysisAdd BLAST | 45 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 55 – 75 | Sequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 19 – 35 | PolarSequence analysisAdd BLAST | 17 | |
Compositional biasi | 649 – 669 | PolyampholyteSequence analysisAdd BLAST | 21 | |
Compositional biasi | 673 – 688 | PolarSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
eggNOGi | KOG1077, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_1_0_1 |
InParanoidi | G1KNY7 |
OMAi | HNELFNC |
TreeFami | TF300308 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 3.30.310.10, 1 hit |
InterProi | View protein in InterPro IPR017104, AP2_complex_asu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR003164, Clathrin_a-adaptin_app_sub_C IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR009028, Coatomer/calthrin_app_sub_C IPR012295, TBP_dom_sf |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02296, Alpha_adaptin_C, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037091, AP2_complex_alpha, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit SSF55711, SSF55711, 1 hit |
i Sequence
Sequence statusi: Complete.
G1KNY7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGFPLVGSVL LGQVSGAERN GASPGSRSST ATMPAVSKGD GMRGLAVFIS
60 70 80 90 100
DIRNCKSKEA EIKRINKELA NIRSKFKGDK ALDGYSKKKY VCKLLFIFLL
110 120 130 140 150
GHDIDFGHME AVNLLSSNKY TEKQIGYLFI SVLVNSNSEL IRLINNAIKN
160 170 180 190 200
DLASRNPTFM CLALHCIANV GSREMAEAFA SEIPRILVAG DTMDSVKQSA
210 220 230 240 250
ALCLLRLYKT SPDLVPMGEW TSRVVHLLND QHMGVVTAAV SLIACLCKKN
260 270 280 290 300
PDDFKTCVSL AVSRLSRIVS SASTDLQDYT YYFVPAPWLS VKLLRLLQCY
310 320 330 340 350
PPPEDAAVKG RLVECLETIL NKAQEPPKSK KVQHSNAKNA ILFEAISLII
360 370 380 390 400
HYDSEPNLLV RACNQLGQFL QHRETNLRYL ALESMCTLAS SEFSHEAVKT
410 420 430 440 450
HIETVINALK TERDVSVRQR AADLLYAMCD RTNAKQIVSE MLSYLETADY
460 470 480 490 500
SIREEIVLKV AILAEKYAVD YSWYVDTILN LIRIAGDYVS EEVWYRVIQI
510 520 530 540 550
VINRDDVQGY AAKTVFEALQ APACHENMVK VGGYILGEFG NLIAGDPRSS
560 570 580 590 600
PLVQFNLLHS KFHLCSVSTR ALLLSTYIKF INLFPETKGT IQEVLRSDSQ
610 620 630 640 650
IRNADVELQQ RAVEYLKLSS IASTDVLATV LEEMPPFPER ESSILAKLKK
660 670 680 690 700
KKGPGAGSEL DDGKKDPNSE INGGMEPSAS TASTPSPSAD LLGLRAAPTS
710 720 730 740 750
ASGAPTSAGN LLVDVFSDSP SATTGLVPGA EENFLRFVCK NNGVLFENQL
760 770 780 790 800
LQIGVKSEFR QNLGRMYLFY GNKTSVQFQS FTPTVSYPGD LQSHLNVQTK
810 820 830 840 850
AVEPLVEGGA QVQQVLNIEC LSDFTEAPLI NIKFRYGGTL QNLTLKLPIT
860 870 880 890 900
VNKFFQATEM QSQDFFQRWK QLSLPQQEAQ KIFKANHPMD SEVTKAKLLG
910 920 930 940 950
FGSALLDKVD PNPDNYVGAG IIQTKALQVG CLLRLEPNAQ AQMYRLTLRT
960
SKESVSKHLC ELLSQQF
Genome annotation databases
Ensembli | ENSACAT00000013593; ENSACAP00000013323; ENSACAG00000013405 |
Similar proteinsi
Cross-referencesi
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 28377.ENSACAP00000013323 |
Genome annotation databases
Ensembli | ENSACAT00000013593; ENSACAP00000013323; ENSACAG00000013405 |
Phylogenomic databases
eggNOGi | KOG1077, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_1_0_1 |
InParanoidi | G1KNY7 |
OMAi | HNELFNC |
TreeFami | TF300308 |
Gene expression databases
Bgeei | ENSACAG00000013405, Expressed in hemipenis and 14 other tissues |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 3.30.310.10, 1 hit |
InterProi | View protein in InterPro IPR017104, AP2_complex_asu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR003164, Clathrin_a-adaptin_app_sub_C IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR009028, Coatomer/calthrin_app_sub_C IPR012295, TBP_dom_sf |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02296, Alpha_adaptin_C, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037091, AP2_complex_alpha, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit SSF55711, SSF55711, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | G1KNY7_ANOCA | |
Accessioni | G1KNY7Primary (citable) accession number: G1KNY7 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 19, 2011 |
Last sequence update: | June 26, 2013 | |
Last modified: | April 7, 2021 | |
This is version 60 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |