UniProtKB - G1KHK4 (G1KHK4_ANOCA)
Protein
ATP-dependent 6-phosphofructokinase
Gene
PFKP
Organism
Anolis carolinensis (Green anole) (American chameleon)
Status
Functioni
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation
Caution
Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
Catalytic activityi
- ATP + β-D-fructose 6-phosphate = ADP + β-D-fructose 1,6-bisphosphate + H+UniRule annotationARBA annotationEC:2.7.1.11UniRule annotationARBA annotation
Cofactori
Mg2+UniRule annotationARBA annotation
Activity regulationi
Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation
: glycolysis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotationARBA annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Hexokinase (HK2), Phosphotransferase (GCK), Hexokinase (HKDC1), Hexokinase (HK3)
- Glucose-6-phosphate isomerase (GPI)
- ATP-dependent 6-phosphofructokinase (PFKP), ATP-dependent 6-phosphofructokinase (PFKM), ATP-dependent 6-phosphofructokinase (PFKL)
- Fructose-bisphosphate aldolase (aldoa), Fructose-bisphosphate aldolase (ALDOC), Fructose-bisphosphate aldolase (ALDOB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 24 | ATP; via amide nitrogenUniRule annotation | 1 | |
Metal bindingi | 118 | Magnesium; catalyticUniRule annotation | 1 | |
Active sitei | 165 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 200 | Substrate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 263 | SubstrateUniRule annotation | 1 | |
Binding sitei | 291 | Substrate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 477 | Allosteric activator fructose 2,6-bisphosphateUniRule annotation | 1 | |
Binding sitei | 572 | Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 635 | Allosteric activator fructose 2,6-bisphosphateUniRule annotation | 1 | |
Binding sitei | 661 | Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 743 | Allosteric activator fructose 2,6-bisphosphateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 87 – 88 | ATPUniRule annotation | 2 | |
Nucleotide bindingi | 117 – 120 | ATPUniRule annotation | 4 |
GO - Molecular functioni
- 6-phosphofructokinase activity Source: GO_Central
- AMP binding Source: GO_Central
- ATP binding Source: GO_Central
- fructose-6-phosphate binding Source: GO_Central
- identical protein binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- monosaccharide binding Source: GO_Central
- protein-containing complex binding Source: Ensembl
GO - Biological processi
- canonical glycolysis Source: GO_Central
- cellular response to leukemia inhibitory factor Source: Ensembl
- fructose 1,6-bisphosphate metabolic process Source: GO_Central
- fructose 6-phosphate metabolic process Source: GO_Central
- glucose catabolic process Source: GO_Central
- protein homotetramerization Source: GO_Central
Keywordsi
Molecular function | Allosteric enzymeUniRule annotation, KinaseUniRule annotationARBA annotation, Transferase |
Biological process | GlycolysisUniRule annotationARBA annotation |
Ligand | ATP-bindingUniRule annotation, MagnesiumUniRule annotationARBA annotation, Metal-bindingUniRule annotationARBA annotation, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00182 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)Short name: ATP-PFKUniRule annotation Short name: PhosphofructokinaseUniRule annotation Alternative name(s): PhosphohexokinaseUniRule annotation |
Gene namesi | Name:PFKPImported |
Organismi | Anolis carolinensis (Green anole) (American chameleon)Imported |
Taxonomic identifieri | 28377 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Iguania › Dactyloidae › Anolis |
Proteomesi |
|
Subcellular locationi
Other locations
- Cytoplasm UniRule annotation
Cytosol
- 6-phosphofructokinase complex Source: GO_Central
Other locations
- membrane Source: GO_Central
Keywords - Cellular componenti
CytoplasmUniRule annotationARBA annotationExpressioni
Gene expression databases
Bgeei | ENSACAG00000008018, Expressed in heart and 15 other tissues |
Interactioni
Subunit structurei
Homo- and heterotetramers.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 28377.ENSACAP00000008104 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 321 | PFKInterPro annotationAdd BLAST | 305 | |
Domaini | 409 – 692 | PFKInterPro annotationAdd BLAST | 284 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 389 | N-terminal catalytic PFK domain 1UniRule annotationAdd BLAST | 389 | |
Regioni | 163 – 165 | Substrate bindingUniRule annotation | 3 | |
Regioni | 207 – 209 | Substrate bindingUniRule annotation | 3 | |
Regioni | 297 – 300 | Substrate bindingUniRule annotation | 4 | |
Regioni | 408 – 786 | C-terminal regulatory PFK domain 2UniRule annotationAdd BLAST | 379 | |
Regioni | 534 – 538 | Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation | 5 | |
Regioni | 579 – 581 | Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation | 3 | |
Regioni | 667 – 670 | Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 608 – 628 | Sequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation
Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.UniRule annotation
Keywords - Domaini
Coiled coilSequence analysisPhylogenomic databases
eggNOGi | KOG2440, Eukaryota |
GeneTreei | ENSGT00940000155002 |
HOGENOMi | CLU_011053_0_0_1 |
InParanoidi | G1KHK4 |
TreeFami | TF300411 |
Family and domain databases
CDDi | cd00764, Eukaryotic_PFK, 1 hit |
HAMAPi | MF_03184, Phosphofructokinase_I_E, 1 hit |
InterProi | View protein in InterPro IPR009161, 6-Pfructokinase_euk IPR022953, ATP_PFK IPR041914, PFK_vert-type IPR015912, Phosphofructokinase_CS IPR000023, Phosphofructokinase_dom IPR035966, PKF_sf |
Pfami | View protein in Pfam PF00365, PFK, 2 hits |
PIRSFi | PIRSF000533, ATP_PFK_euk, 1 hit |
PRINTSi | PR00476, PHFRCTKINASE |
SUPFAMi | SSF53784, SSF53784, 2 hits |
TIGRFAMsi | TIGR02478, 6PF1K_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00433, PHOSPHOFRUCTOKINASE, 2 hits |
i Sequence
Sequence statusi: Complete.
G1KHK4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPDKKFFENL SGAGKAIGVL TSGGDAQGMN AAVRAVVRMG IYVEAKVYFI
60 70 80 90 100
YEGYEGMVDG GENIVEVTWE SVSSILQVGG TVIGSARCKA FRTREGRLKA
110 120 130 140 150
ACNLVKCGIT NLCVIGGDGS LTGANIFRTE WSGLLEELAS QGEIDEEARK
160 170 180 190 200
KYAYLNIVGM VGSIDNDFCG TDMTIGTDSA LHRIIEVVDA IMTTAQSHQR
210 220 230 240 250
TFVLEVMGRH CGYLALVSAL ACGADYVFIP EYPPEEGWED HMCSRLSENR
260 270 280 290 300
ARKKRLNIII VAEGAIDSNN QPITSEQVKD LVVQRLGYDT RVTILGHVQR
310 320 330 340 350
GGTPSAFDRI LASRMGVEAV LALLEATPET PACVVSLSGN QAVRLPLMEC
360 370 380 390 400
VQMTQDVQKA MDEKNFSDAV QLRGRSFQNN LNTYKLLSTK KTASELPTDD
410 420 430 440 450
ISYNTNFNVA VLNVGAPAAG MNAAVRAAVR IGITEGHKMF AVTDGFEGFS
460 470 480 490 500
KGQIKEISWG DVGGWTGQGG SILGTKRTLP AKHMEKIAEQ MRNHTINALL
510 520 530 540 550
VIGGFEAYLG LLELSAAREK YDEFCIPMVM VPATVSNNVP GSDFSIGADT
560 570 580 590 600
ALNTITDTCD RIKLSASGTK RRVFIIETMG GYCGYLANMG ALAAGADAAY
610 620 630 640 650
IFEEKFDIRE LQANVEHLTE KMKTSIQRGL VLRNENSSEN YTTDFIYQLY
660 670 680 690 700
SEEGKGVFDC RKNVLGHMQQ GGAPSPFDRN FGTKISAKAV LWLSKKLKES
710 720 730 740 750
YRRGEGRVFA NTDDSVCLLG MRRRNLIFQP VIELKGDTDF EHRIPKEQWW
760 770 780
LKLRPLMKIL AKYKTSYDIS DAGQLEHVHR PKPVEA
Genome annotation databases
Ensembli | ENSACAT00000008276; ENSACAP00000008104; ENSACAG00000008018 |
Similar proteinsi
Cross-referencesi
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 28377.ENSACAP00000008104 |
Genome annotation databases
Ensembli | ENSACAT00000008276; ENSACAP00000008104; ENSACAG00000008018 |
Phylogenomic databases
eggNOGi | KOG2440, Eukaryota |
GeneTreei | ENSGT00940000155002 |
HOGENOMi | CLU_011053_0_0_1 |
InParanoidi | G1KHK4 |
TreeFami | TF300411 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00182 |
Gene expression databases
Bgeei | ENSACAG00000008018, Expressed in heart and 15 other tissues |
Family and domain databases
CDDi | cd00764, Eukaryotic_PFK, 1 hit |
HAMAPi | MF_03184, Phosphofructokinase_I_E, 1 hit |
InterProi | View protein in InterPro IPR009161, 6-Pfructokinase_euk IPR022953, ATP_PFK IPR041914, PFK_vert-type IPR015912, Phosphofructokinase_CS IPR000023, Phosphofructokinase_dom IPR035966, PKF_sf |
Pfami | View protein in Pfam PF00365, PFK, 2 hits |
PIRSFi | PIRSF000533, ATP_PFK_euk, 1 hit |
PRINTSi | PR00476, PHFRCTKINASE |
SUPFAMi | SSF53784, SSF53784, 2 hits |
TIGRFAMsi | TIGR02478, 6PF1K_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00433, PHOSPHOFRUCTOKINASE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | G1KHK4_ANOCA | |
Accessioni | G1KHK4Primary (citable) accession number: G1KHK4 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | October 19, 2011 |
Last sequence update: | June 26, 2013 | |
Last modified: | October 7, 2020 | |
This is version 64 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |