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Protein

Alpha-latrotoxin-Lh1a

Gene
N/A
Organism
Latrodectus hasseltii (Redback spider)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Presynaptic neurotoxin that induces exhaustive neurotransmitter release from vertebrate (but not invertebrate) nerve terminals and endocrine cells. Binds to neurexin-1-alpha (NRXN1), adhesion G protein-coupled receptor L1 (ADGRL1, also known as latrophilin-1), and receptor-type tyrosine-protein phosphatase S (PTPRS), and induces neurotransmitter exocytosis through two calcium-dependent mechanisms (membrane pore formation and signaling via latrophilin) and a yet to be defined calcium-independent mechanism (By similarity). Induces rapid muscle contracture and loss of twitch tension when added to the isolated and indirectly stimulated chick biventer cervicis nerve-muscle preparation.By similarity1 Publication

Miscellaneous

Is the main neurotoxin responsible for the human envenomation syndrome known as latrodectism that results from bites by Latrodectus species.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNeurotoxin, Presynaptic neurotoxin, Toxin
Biological processExocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-latrotoxin-Lh1a
Short name:
Alpha-LTX-Lh1a
Alternative name(s):
Alpha-latrotoxin
OrganismiLatrodectus hasseltii (Redback spider)
Taxonomic identifieri256736 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAraneoideaTheridiidaeLatrodectus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Secreted, Target cell membrane, Target membrane

Pathology & Biotechi

Toxic dosei

LD50is 20 µg/kg by subcutaneous injection into mice.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 7Sequence analysis›7
ChainiPRO_50007835708 – 1187Alpha-latrotoxin-Lh1aAdd BLAST1180
PropeptideiPRO_00004159311188 – ›1351By similarityAdd BLAST›164

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi401 ↔ 1054Curated

Post-translational modificationi

Processed by furin-like proteases at both the N- and C-termini.

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homotetramer; Homotetramerization is triggered by calcium and is responsible for non-vesicular release and calcium-dependent vesicular exocytosis.By similarity

Structurei

3D structure databases

SMRiG0LXV8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati446 – 477ANK 1Add BLAST32
Repeati478 – 509ANK 2Add BLAST32
Repeati513 – 542ANK 3Add BLAST30
Repeati547 – 577ANK 4Add BLAST31
Repeati581 – 610ANK 5Add BLAST30
Repeati614 – 644ANK 6Add BLAST31
Repeati648 – 678ANK 7Add BLAST31
Repeati683 – 711ANK 8Add BLAST29
Repeati717 – 746ANK 9Add BLAST30
Repeati750 – 779ANK 10Add BLAST30
Repeati783 – 812ANK 11Add BLAST30
Repeati816 – 846ANK 12Add BLAST31
Repeati850 – 879ANK 13Add BLAST30
Repeati883 – 912ANK 14Add BLAST30
Repeati916 – 945ANK 15Add BLAST30
Repeati959 – 991ANK 16Add BLAST33
Repeati992 – 1019ANK 17Add BLAST28
Repeati1023 – 1052ANK 18Add BLAST30
Repeati1056 – 1085ANK 19Add BLAST30
Repeati1089 – 1119ANK 20Add BLAST31
Repeati1125 – 1154ANK 21Add BLAST30
Repeati1158 – 1187ANK 22Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 7Furin-like endopeptidase recognition region4
Regioni1184 – 1187Furin-like endopeptidase recognition region4

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Signal, Transmembrane

Family and domain databases

CDDicd00204 ANK, 6 hits
Gene3Di1.25.40.20, 4 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 5 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 20 hits
SUPFAMiSSF48403 SSF48403, 3 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 10 hits

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

G0LXV8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
SLVRMRREGE EDLTLEEKAE LCSELELQQK YVDIGSNIIG DLSSLPIVGK
60 70 80 90 100
IVGTIAAAAM AVTHVASGRL DIEQTLGGCS DVPFDQIKEI LEERFNEIDR
110 120 130 140 150
KLESHSAALE EITKLVEKSI SAVEKTRKQM NKRFDEVMRS IQDAKVSPLV
160 170 180 190 200
SKINNFARYF DTEKERIRGL KLSDYILKLE EPNGILLHFK ESRTPRDDSL
210 220 230 240 250
QAPLFSIIQE RYAVPKSIDD ELAFKVLYAL LYGTQTYVSV MFFLLEQYSF
260 270 280 290 300
LANHYYEKGD LEKYDEYFNS LNNVFLDFKS SLVGTGTSNN EGLLDRVLQV
310 320 330 340 350
LVTVKNSEFL GLEKNGVNEM LNEKINLFNK IKVEIEGKQR MTLSETPENF
360 370 380 390 400
AQISFDKDIT TPIGDWRDGR EVRYAVQYAS ETLFSKISHW SDPVGVREKA
410 420 430 440 450
CPTLRMPVDQ TRRNILVFRK FDSSKPQLVG EITPYQSNFI DIDRDLYNTA
460 470 480 490 500
NNPDSAVGFK EFTKLNYDGA NIRATFEQGR TVFHAAAKSG NSRIMIGLTF
510 520 530 540 550
LVKSNELNQP DKKGYTPIHV AADSGNAGIV NLLIQRGVSI NSKTYNFLQT
560 570 580 590 600
PLHLAAQRGF VTTFQRLMES PEININERDK DGFTPLHYAV RGGERILEAF
610 620 630 640 650
INQIRIDLNA KSNKGLTPFH LAIIKDDWPV ASTLLGSKKV DVNAVDENNM
660 670 680 690 700
TALHYAAILG YLETTKQLIN LKEINADVVS SPGLLSALHY AILYKHDDVA
710 720 730 740 750
SFLLRSSNVN VNLKALGGIT PLHLAVIQGR TQILSLMFDI GVNIEQQTDE
760 770 780 790 800
KYTPLHLAAM SKYPELIQIL LDQGSNFEAK TNSGATPLHL ATFKGKSKAA
810 820 830 840 850
LILLNNEVNW RDTDENGQMP IHGAAMNGLL DVAQAIISID ATVLDIKDKN
860 870 880 890 900
SDTPLNLAAQ KSHIDVIKYF IDQGADINTR NKTGHAPLLA FSKKGNLDMV
910 920 930 940 950
KYLFDKNANV YIADNDGINF FYYAVRNGHL NIVKYAMSEK DKFEWSNIDN
960 970 980 990 1000
NRRDECPKEE CAISHFAVCD AVQFDKIEIV KYFVTTLGNF AICGPLHQAA
1010 1020 1030 1040 1050
RYGHLDIEKY LVEEEDLNVD GSKPDTPLCY ASENGHLAVV QYLVSNGAKV
1060 1070 1080 1090 1100
NHDCGNGMTA IDKAITKNHL QVVQFLAANG VDFRRKNKLG ATPFLTAVSE
1110 1120 1130 1140 1150
NAFDIAEYLI RENRQDIDIN EQNVDKETAL HLAVYYKNLQ MIKLLVKYGI
1160 1170 1180 1190 1200
DMTIRNAYDK TALDIATDLK NSNIVEYLKT KSGKFRREYK SSYGEHSLLQ
1210 1220 1230 1240 1250
TNKISSFIDG KNIEHDHPQF INADNESSQL FSDTASNIDV IGPLLLIDVL
1260 1270 1280 1290 1300
IRYFSKQGYI SKESDSASDG ITQAAALSIT EKFEDVLNSL HNESAKEQVD
1310 1320 1330 1340 1350
LAEVHGKVYA ALKSGRNSQI HPILCSSLKS ISTLKPEDME KLGSVIMNSH

S
Length:1,351
Mass (Da):151,407
Last modified:March 21, 2012 - v2
Checksum:i1200C1B85D42A908
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence uncertaintyi1202Assigned by comparison with orthologs1
Sequence uncertaintyi1215Assigned by comparison with orthologs1
Non-terminal residuei13511

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR851877 Genomic DNA Translation: CCA64564.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR851877 Genomic DNA Translation: CCA64564.1

3D structure databases

SMRiG0LXV8
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00204 ANK, 6 hits
Gene3Di1.25.40.20, 4 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF12796 Ank_2, 5 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 20 hits
SUPFAMiSSF48403 SSF48403, 3 hits
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 10 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLATA_LATHA
AccessioniPrimary (citable) accession number: G0LXV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 21, 2012
Last modified: June 20, 2018
This is version 27 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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