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Protein

Beta-agarase A

Gene

agaA

Organism
Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place.1 Publication

Catalytic activityi

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.1 Publication

Kineticsi

kcat is 150 sec(-1).
  1. KM=2 mM for agarose1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei73Substrate1 Publication1
    Binding sitei144Substrate1 Publication1
    Active sitei147Nucleophile1 Publication1
    Active sitei152Proton donor1 Publication1
    Binding sitei176Substrate1 Publication1
    Binding sitei271Substrate1 Publication1

    GO - Molecular functioni

    • beta-agarase activity Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16651
    BRENDAi3.2.1.81 7557
    SABIO-RKiG0L322

    Protein family/group databases

    CAZyiGH16 Glycoside Hydrolase Family 16

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-agarase A (EC:3.2.1.81)
    Cleaved into the following chain:
    Gene namesi
    Name:agaA
    Ordered Locus Names:zobellia_4203
    OrganismiZobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)
    Taxonomic identifieri63186 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeZobellia
    Proteomesi
    • UP000008898 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi147E → S: Abolishes beta-agarase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 19Sequence analysisAdd BLAST19
    ChainiPRO_500005526820 – 539Beta-agarase AAdd BLAST520
    ChainiPRO_500005526920 – 295Beta-agarase A catalytic chainAdd BLAST276

    Post-translational modificationi

    Proteolytically cleaved into mature beta-agarase A catalytic chain (AgaAc).1 Publication

    Expressioni

    Inductioni

    When cells are grown with the low sulfated agar.1 Publication

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Structurei

    Secondary structure

    1539
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SMRiG0L322
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini21 – 289GH16PROSITE-ProRule annotationAdd BLAST269

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni82 – 92Substrate binding1 PublicationAdd BLAST11
    Regioni96 – 98Substrate binding1 Publication3

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    KOiK01219

    Family and domain databases

    CDDicd02178 GH16_beta_agarase, 1 hit
    InterProiView protein in InterPro
    IPR016287 Beta_agarase
    IPR013320 ConA-like_dom_sf
    IPR008979 Galactose-bd-like_sf
    IPR000757 GH16
    IPR026444 Secre_tail
    PfamiView protein in Pfam
    PF00722 Glyco_hydro_16, 1 hit
    SUPFAMiSSF49785 SSF49785, 1 hit
    SSF49899 SSF49899, 1 hit
    TIGRFAMsiTIGR04183 Por_Secre_tail, 1 hit
    PROSITEiView protein in PROSITE
    PS51762 GH16_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    G0L322-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKKNYLLLYF IFLLCGSIAA QDWNGIPVPA NPGNGMTWQL QDNVSDSFNY
    60 70 80 90 100
    TSSEGNRPTA FTSKWKPSYI NGWTGPGSTI FNAPQAWTNG SQLAIQAQPA
    110 120 130 140 150
    GNGKSYNGII TSKNKIQYPV YMEIKAKIMD QVLANAFWTL TDDETQEIDI
    160 170 180 190 200
    MEGYGSDRGG TWFAQRMHLS HHTFIRNPFT DYQPMGDATW YYNGGTPWRS
    210 220 230 240 250
    AYHRYGCYWK DPFTLEYYID GVKVRTVTRA EIDPNNHLGG TGLNQATNII
    260 270 280 290 300
    IDCENQTDWR PAATQEELAD DSKNIFWVDW IRVYKPVAVS GGGNNGNDGA
    310 320 330 340 350
    TEFQYDLGTD TSAVWPGYTR VSNTTRAGNF GWANTNDIGS RDRGASNGRN
    360 370 380 390 400
    NINRDINFSS QTRFFTQDLS NGTYNVLITF GDTYARKNMN VAAEGQNKLT
    410 420 430 440 450
    NINTNAGQYV SRSFDVNVND GKLDLRFSVG NGGDVWSITR IWIRKVTSNS
    460 470 480 490 500
    ANLLAAKGLT LEDPVETTEF LYPNPAKTDD FVTVPNSEIG SSIIIYNSAG
    510 520 530
    QVVKKVSVVS ENQKISLEGF AKGMYFINLN GQSTKLIVQ
    Length:539
    Mass (Da):60,020
    Last modified:October 19, 2011 - v1
    Checksum:i8D60A87DBF009A44
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti84P → A in AAF21820 (PubMed:15456406).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF098954 Genomic DNA Translation: AAF21820.1
    FP476056 Genomic DNA Translation: CAZ98338.1

    Genome annotation databases

    EnsemblBacteriaiCAZ98338; CAZ98338; ZOBELLIA_4203
    KEGGizga:ZOBELLIA_4203

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF098954 Genomic DNA Translation: AAF21820.1
    FP476056 Genomic DNA Translation: CAZ98338.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1O4YX-ray1.48A20-295[»]
    1URXX-ray1.70A20-290[»]
    SMRiG0L322
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH16 Glycoside Hydrolase Family 16

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAZ98338; CAZ98338; ZOBELLIA_4203
    KEGGizga:ZOBELLIA_4203

    Phylogenomic databases

    KOiK01219

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16651
    BRENDAi3.2.1.81 7557
    SABIO-RKiG0L322

    Family and domain databases

    CDDicd02178 GH16_beta_agarase, 1 hit
    InterProiView protein in InterPro
    IPR016287 Beta_agarase
    IPR013320 ConA-like_dom_sf
    IPR008979 Galactose-bd-like_sf
    IPR000757 GH16
    IPR026444 Secre_tail
    PfamiView protein in Pfam
    PF00722 Glyco_hydro_16, 1 hit
    SUPFAMiSSF49785 SSF49785, 1 hit
    SSF49899 SSF49899, 1 hit
    TIGRFAMsiTIGR04183 Por_Secre_tail, 1 hit
    PROSITEiView protein in PROSITE
    PS51762 GH16_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAGAA_ZOBGA
    AccessioniPrimary (citable) accession number: G0L322
    Secondary accession number(s): Q9RGX9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
    Last sequence update: October 19, 2011
    Last modified: February 28, 2018
    This is version 39 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
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