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Protein

Riboflavin biosynthesis protein RibBA

Gene

ribBA

Organism
Alicyclobacillus acidocaldarius (strain Tc-4-1) (Bacillus acidocaldarius)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotationSAAS annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin biosynthesis protein RibBA (ribBA)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.SAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Riboflavin biosynthesis protein RibBA (ribBA)
  2. Riboflavin biosynthesis protein RibD (ribD)
  3. Riboflavin biosynthesis protein RibD (ribD)
  4. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Magnesium or manganese 1UniRule annotation1
Metal bindingi26Magnesium or manganese 2UniRule annotation1
Binding sitei30D-ribulose 5-phosphateUniRule annotation1
Sitei123Essential for DHBP synthase activityUniRule annotation1
Metal bindingi140Magnesium or manganese 2UniRule annotation1
Binding sitei161D-ribulose 5-phosphateUniRule annotation1
Sitei161Essential for DHBP synthase activityUniRule annotation1
Metal bindingi254Zinc; catalyticUniRule annotation1
Metal bindingi265Zinc; catalyticUniRule annotation1
Metal bindingi267Zinc; catalyticUniRule annotation1
Binding sitei270GTPUniRule annotation1
Binding sitei314GTPUniRule annotation1
Active sitei326Proton acceptor; for GTP cyclohydrolase activityUniRule annotation1
Active sitei328Nucleophile; for GTP cyclohydrolase activityUniRule annotation1
Binding sitei349GTPUniRule annotation1
Binding sitei354GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi249 – 253GTPUniRule annotation5
Nucleotide bindingi292 – 294GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported, LyaseUniRule annotation, Multifunctional enzymeUniRule annotation
Biological processRiboflavin biosynthesisUniRule annotationSAAS annotation
LigandGTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciAACI1048834:G1GZ5-888-MONOMER
UniPathwayi
UPA00275;UER00399

UPA00275;UER00400

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Including the following 2 domains:
3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation)
Short name:
DHBP synthaseUniRule annotation
GTP cyclohydrolase-2UniRule annotation (EC:3.5.4.25UniRule annotation)
Alternative name(s):
GTP cyclohydrolase IIUniRule annotation
Gene namesi
Name:ribBAUniRule annotationImported
Ordered Locus Names:TC41_0948Imported
OrganismiAlicyclobacillus acidocaldarius (strain Tc-4-1) (Bacillus acidocaldarius)Imported
Taxonomic identifieri1048834 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesAlicyclobacillaceaeAlicyclobacillus
Proteomesi
  • UP000000292 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi1048834.TC41_0948

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 370GTP_cyclohydro2InterPro annotationAdd BLAST166

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 198DHBP synthaseUniRule annotationAdd BLAST198
Regioni25 – 26D-ribulose 5-phosphate bindingUniRule annotation2
Regioni137 – 141D-ribulose 5-phosphate bindingUniRule annotation5
Regioni199 – 396GTP cyclohydrolase IIUniRule annotationAdd BLAST198

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotationSAAS annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C66 Bacteria
COG0108 LUCA
COG0807 LUCA
KOiK14652
OMAiGCTTGIS

Family and domain databases

CDDicd00641 GTP_cyclohydro2, 1 hit
Gene3Di3.40.50.10990, 1 hit
HAMAPiMF_00179 RibA, 1 hit
MF_00180 RibB, 1 hit
MF_01283 RibBA, 1 hit
InterProiView protein in InterPro
IPR017945 DHBP_synth_RibB-like_a/b_dom
IPR000422 DHBP_synthase_RibB
IPR032677 GTP_cyclohydro_II
IPR000926 RibA
IPR036144 RibA-like_sf
IPR016299 Riboflavin_synth_RibBA
PfamiView protein in Pfam
PF00926 DHBP_synthase, 1 hit
PF00925 GTP_cyclohydro2, 1 hit
SUPFAMiSSF142695 SSF142695, 1 hit
SSF55821 SSF55821, 1 hit
TIGRFAMsiTIGR00505 ribA, 1 hit
TIGR00506 ribB, 1 hit

Sequencei

Sequence statusi: Complete.

F8IFJ9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNTIEEALDD LRQGRVVIVV DDEDRENEGD FVALGSRATP DVVNFMITHG
60 70 80 90 100
RGLLCVPITE ARAKRLGLEA MYERNTDNYG TAFTVSVDLV TTHTGISAFE
110 120 130 140 150
RAETIAAIVS EDTGPDDFRK PGHVFPLVAK PGGVLRRAGH TEAAVDLARL
160 170 180 190 200
CGEPEVGVIC EILNEDGTMA RLPQLEQVAE RFGLKLITIA DLIAYRKRTE
210 220 230 240 250
KLVRRAAEAE LPTMYGHFRA VVYESEIDDK EHIALVMGDV SDGEPTLVRV
260 270 280 290 300
HSECLTGDAF GSLRCDCGPQ LHAALRQIAR EGKGVLLYLR QEGRGIGLLN
310 320 330 340 350
KIRAYALQDQ GLDTVEANHR LGFADDLRDY GIGAQILRDL GIRKMRLLTN
360 370 380 390
NVRKIKGIAG HGLEVVERVP LEVDRNPYNE AYLRTKRDKL GHLLNL
Length:396
Mass (Da):43,795
Last modified:September 21, 2011 - v1
Checksum:iDFE42CA87B9A4A42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002902 Genomic DNA Translation: AEJ42901.1
RefSeqiWP_014463801.1, NC_017167.1

Genome annotation databases

EnsemblBacteriaiAEJ42901; AEJ42901; TC41_0948
KEGGiaad:TC41_0948
PATRICifig|1048834.4.peg.904

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002902 Genomic DNA Translation: AEJ42901.1
RefSeqiWP_014463801.1, NC_017167.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1048834.TC41_0948

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEJ42901; AEJ42901; TC41_0948
KEGGiaad:TC41_0948
PATRICifig|1048834.4.peg.904

Phylogenomic databases

eggNOGiENOG4105C66 Bacteria
COG0108 LUCA
COG0807 LUCA
KOiK14652
OMAiGCTTGIS

Enzyme and pathway databases

UniPathwayi
UPA00275;UER00399

UPA00275;UER00400

BioCyciAACI1048834:G1GZ5-888-MONOMER

Family and domain databases

CDDicd00641 GTP_cyclohydro2, 1 hit
Gene3Di3.40.50.10990, 1 hit
HAMAPiMF_00179 RibA, 1 hit
MF_00180 RibB, 1 hit
MF_01283 RibBA, 1 hit
InterProiView protein in InterPro
IPR017945 DHBP_synth_RibB-like_a/b_dom
IPR000422 DHBP_synthase_RibB
IPR032677 GTP_cyclohydro_II
IPR000926 RibA
IPR036144 RibA-like_sf
IPR016299 Riboflavin_synth_RibBA
PfamiView protein in Pfam
PF00926 DHBP_synthase, 1 hit
PF00925 GTP_cyclohydro2, 1 hit
SUPFAMiSSF142695 SSF142695, 1 hit
SSF55821 SSF55821, 1 hit
TIGRFAMsiTIGR00505 ribA, 1 hit
TIGR00506 ribB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiF8IFJ9_ALIAT
AccessioniPrimary (citable) accession number: F8IFJ9
Entry historyiIntegrated into UniProtKB/TrEMBL: September 21, 2011
Last sequence update: September 21, 2011
Last modified: September 12, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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