UniProtKB - F7E2E6 (F7E2E6_HORSE)
Protein
Dopamine beta-hydroxylase
Gene
DBH
Organism
Equus caballus (Horse)
Status
Functioni
Conversion of dopamine to noradrenaline.ARBA annotation
Catalytic activityi
- dopamine + 2 L-ascorbate + O2 = (R)-noradrenaline + H2O + 2 monodehydro-L-ascorbate radicalARBA annotationEC:1.14.17.1ARBA annotation
Cofactori
Cu2+ARBA annotation
: (R)-noradrenaline biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (R)-noradrenaline from dopamine.ARBA annotationProteins known to be involved in this subpathway in this organism are:
- Dopamine beta-hydroxylase (DBH), Dopamine beta-hydroxylase (DBH), Dopamine beta-hydroxylase (DBH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-noradrenaline from dopamine, the pathway (R)-noradrenaline biosynthesis and in Catecholamine biosynthesis.
GO - Molecular functioni
- copper ion binding Source: GO_Central
- dopamine beta-monooxygenase activity Source: GO_Central
- L-ascorbic acid binding Source: UniProtKB-KW
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced ascorbate as one donor, and incorporation of one atom of oxygen Source: GO_Central
GO - Biological processi
- behavioral response to ethanol Source: Ensembl
- blood vessel remodeling Source: Ensembl
- cytokine production Source: Ensembl
- dopamine catabolic process Source: GO_Central
- fear response Source: Ensembl
- glucose homeostasis Source: Ensembl
- homoiothermy Source: Ensembl
- leukocyte mediated immunity Source: Ensembl
- leukocyte migration Source: Ensembl
- locomotory behavior Source: Ensembl
- maternal behavior Source: Ensembl
- memory Source: Ensembl
- norepinephrine biosynthetic process Source: GO_Central
- octopamine biosynthetic process Source: GO_Central
- positive regulation of cold-induced thermogenesis Source: Ensembl
- positive regulation of vasoconstriction Source: Ensembl
- regulation of cell population proliferation Source: Ensembl
- regulation of extrinsic apoptotic signaling pathway Source: Ensembl
- response to amphetamine Source: Ensembl
- response to pain Source: Ensembl
- visual learning Source: Ensembl
Keywordsi
Molecular function | MonooxygenaseARBA annotation, Oxidoreductase |
Ligand | CopperARBA annotation, Metal-bindingARBA annotation, Vitamin CARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00748;UER00735 |
Names & Taxonomyi
Protein namesi | Recommended name: Dopamine beta-hydroxylaseARBA annotation (EC:1.14.17.1ARBA annotation)Alternative name(s): Soluble dopamine beta-hydroxylaseARBA annotation |
Gene namesi | Name:DBHImported |
Organismi | Equus caballus (Horse)Imported |
Taxonomic identifieri | 9796 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:17016, DBH |
Subcellular locationi
Other locations
- secretory vesicle lumen ARBA annotation
- secretory vesicle membrane ARBA annotation; Single-pass type II membrane protein ARBA annotation
- chromaffin granule lumen ARBA annotation
- chromaffin granule membrane ARBA annotation; Single-pass type II membrane protein ARBA annotation
Cytoskeleton
- centriolar satellite Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum Source: Ensembl
Extracellular region or secreted
- extracellular space Source: GO_Central
Other locations
- chromaffin granule lumen Source: UniProtKB-SubCell
- chromaffin granule membrane Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
- secretory granule membrane Source: GO_Central
- transport vesicle membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
MembraneExpressioni
Gene expression databases
Bgeei | ENSECAG00000019582, Expressed in multi-cellular organism and 3 other tissues |
ExpressionAtlasi | F7E2E6, baseline |
Interactioni
Subunit structurei
Homotetramer; composed of two disulfide-linked dimers.
ARBA annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 166 | DOMONInterPro annotationAdd BLAST | 117 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 586 – 610 | DisorderedSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the copper type II ascorbate-dependent monooxygenase family.ARBA annotation
Keywords - Domaini
Transmembrane, Transmembrane helixARBA annotationPhylogenomic databases
GeneTreei | ENSGT00530000063085 |
HOGENOMi | CLU_017939_3_0_1 |
TreeFami | TF320698 |
Family and domain databases
Gene3Di | 2.60.120.230, 1 hit 2.60.120.310, 1 hit |
InterProi | View protein in InterPro IPR014784, Cu2_ascorb_mOase-like_C IPR020611, Cu2_ascorb_mOase_CS-1 IPR014783, Cu2_ascorb_mOase_CS-2 IPR000323, Cu2_ascorb_mOase_N IPR036939, Cu2_ascorb_mOase_N_sf IPR024548, Cu2_monoox_C IPR000945, DBH-like IPR005018, DOMON_domain IPR008977, PHM/PNGase_F_dom_sf IPR028460, Tbh/DBH |
PANTHERi | PTHR10157, PTHR10157, 1 hit |
Pfami | View protein in Pfam PF03712, Cu2_monoox_C, 1 hit PF01082, Cu2_monooxygen, 1 hit PF03351, DOMON, 1 hit |
PRINTSi | PR00767, DBMONOXGNASE |
SMARTi | View protein in SMART SM00664, DoH, 1 hit |
SUPFAMi | SSF49742, SSF49742, 2 hits |
PROSITEi | View protein in PROSITE PS00084, CU2_MONOOXYGENASE_1, 1 hit PS00085, CU2_MONOOXYGENASE_2, 1 hit PS50836, DOMON, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
F7E2E6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVPSPSVRE AASMYGTAVA IFLVILVAAL QGSEPPESPF PYRIPLDPEG
60 70 80 90 100
TLELSWNVSY VQETIHFQLL VRELKAGVLF GMSDRGELEN ADLVVLWTDG
110 120 130 140 150
DSAYFGDAWS DQKGWIHLDA QQDYQLLRAQ RTPEGLSLLF KRPFGTCDPK
160 170 180 190 200
DYLIEDGTVH LVYGILEEPF WSLEAINTSA LHTGLQRVQL LKPNVSVPAL
210 220 230 240 250
PADMRTMEVR APDVLVPGQE TTYWCYITEL PDGFPRHHIV MYEPIVTEGN
260 270 280 290 300
EALVHHMEVF QCATEFESFP QFNGPCDSKM KPSRLNYCRH VLAAWALGAK
310 320 330 340 350
AFYYPEEAGL AFGGAGSSRF LRLEVHYHNP LKIEGRRDSS GIRLYYTATL
360 370 380 390 400
RRFDAGIMEL GLVYTPVMAI PPQETAFVLT GYCTDKCTQL ALPPSGIHIF
410 420 430 440 450
ASQLHTHLTG RKVVTVLARD GREREVVNRD DHYSPHFQEI RMLKKVVSVH
460 470 480 490 500
PGDVLITSCT YNTEDRKLAT VGGFGILEEM CVNYVHYYPQ TQLELCKSAV
510 520 530 540 550
DPGFLQKYFH FVNRFNGEEV CTCPQASVPE QFATVPWNSF NRQVLSALYG
560 570 580 590 600
FAPISMHCNK SSAVRFQGEW NLQPLPEIIS KLEEPTPQCP ASRGRSPAGP
610
TVVDIGGGKG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q2LGW7 | A0A3Q2LGW7_HORSE | Dopamine beta-hydroxylase | DBH | 602 | Annotation score: |
Sequence databases
RefSeqi | NP_001075239.2, NM_001081770.2 |
Genome annotation databases
Ensembli | ENSECAT00000021063; ENSECAP00000017330; ENSECAG00000019582 |
GeneIDi | 791247 |
KEGGi | ecb:791247 |
Similar proteinsi
Cross-referencesi
Sequence databases
RefSeqi | NP_001075239.2, NM_001081770.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
Ensembli | ENSECAT00000021063; ENSECAP00000017330; ENSECAG00000019582 |
GeneIDi | 791247 |
KEGGi | ecb:791247 |
Organism-specific databases
CTDi | 1621 |
VGNCi | VGNC:17016, DBH |
Phylogenomic databases
GeneTreei | ENSGT00530000063085 |
HOGENOMi | CLU_017939_3_0_1 |
TreeFami | TF320698 |
Enzyme and pathway databases
UniPathwayi | UPA00748;UER00735 |
Gene expression databases
Bgeei | ENSECAG00000019582, Expressed in multi-cellular organism and 3 other tissues |
ExpressionAtlasi | F7E2E6, baseline |
Family and domain databases
Gene3Di | 2.60.120.230, 1 hit 2.60.120.310, 1 hit |
InterProi | View protein in InterPro IPR014784, Cu2_ascorb_mOase-like_C IPR020611, Cu2_ascorb_mOase_CS-1 IPR014783, Cu2_ascorb_mOase_CS-2 IPR000323, Cu2_ascorb_mOase_N IPR036939, Cu2_ascorb_mOase_N_sf IPR024548, Cu2_monoox_C IPR000945, DBH-like IPR005018, DOMON_domain IPR008977, PHM/PNGase_F_dom_sf IPR028460, Tbh/DBH |
PANTHERi | PTHR10157, PTHR10157, 1 hit |
Pfami | View protein in Pfam PF03712, Cu2_monoox_C, 1 hit PF01082, Cu2_monooxygen, 1 hit PF03351, DOMON, 1 hit |
PRINTSi | PR00767, DBMONOXGNASE |
SMARTi | View protein in SMART SM00664, DoH, 1 hit |
SUPFAMi | SSF49742, SSF49742, 2 hits |
PROSITEi | View protein in PROSITE PS00084, CU2_MONOOXYGENASE_1, 1 hit PS00085, CU2_MONOOXYGENASE_2, 1 hit PS50836, DOMON, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F7E2E6_HORSE | |
Accessioni | F7E2E6Primary (citable) accession number: F7E2E6 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 27, 2011 |
Last sequence update: | April 10, 2019 | |
Last modified: | December 2, 2020 | |
This is version 58 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |