UniProtKB - F7CBX0 (F7CBX0_HORSE)
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Gene
DDOST
Organism
Equus caballus (Horse)
Status
Functioni
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).UniRule annotation
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.UniRule annotationARBA annotationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
- enzyme activator activity Source: Ensembl
GO - Biological processi
- protein glycosylation Source: UniProtKB
- protein N-linked glycosylation via asparagine Source: GO_Central
- regulation of protein stability Source: Ensembl
- response to cytokine Source: Ensembl
- T cell activation Source: Ensembl
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Names & Taxonomyi
Protein namesi | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitUniRule annotationARBA annotationShort name: Oligosaccharyl transferase 48 kDa subunitUniRule annotation |
Gene namesi | Name:DDOSTImported |
Organismi | Equus caballus (Horse)Imported |
Taxonomic identifieri | 9796 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:17080, DDOST |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotationARBA annotation; Single-pass type I membrane protein UniRule annotationARBA annotation
Other locations
- Membrane ARBA annotation; Single-pass type I membrane protein ARBA annotation
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- oligosaccharyltransferase complex Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-UniRule
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 425 – 446 | HelicalUniRule annotationAdd BLAST | 22 |
Keywords - Cellular componenti
Endoplasmic reticulumUniRule annotationARBA annotation, MembraneExpressioni
Gene expression databases
Bgeei | ENSECAG00000019546, Expressed in trophoblast and 22 other tissues |
ExpressionAtlasi | F7CBX0, baseline |
Interactioni
Subunit structurei
Component of the oligosaccharyltransferase (OST) complex.
UniRule annotationFamily & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixUniRule annotationARBA annotationPhylogenomic databases
GeneTreei | ENSGT00390000017294 |
HOGENOMi | CLU_031804_0_0_1 |
InParanoidi | F7CBX0 |
TreeFami | TF314821 |
Family and domain databases
InterProi | View protein in InterPro IPR005013, DDOST_48_kDa_subunit |
PANTHERi | PTHR10830, PTHR10830, 1 hit |
Pfami | View protein in Pfam PF03345, DDOST_48kD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
F7CBX0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
WHFRCPGAWF FGRRRKMEHG TATRAWSLLW LLLPLVGPVC ASGLRTLVLL
60 70 80 90 100
DNLNLRETHS LFFRSLKDRG FELTFKTADD PSLSLIKYGE FLYDNLIIFS
110 120 130 140 150
PSVEDFGGNI NVETISTFID GGGSVLVAAS SDIGDPLREL GSECGIEFDE
160 170 180 190 200
EKTAVIDHHN YDISDLGQHT LIVADTENLL KAPTIVGKSS LNPILFRGVG
210 220 230 240 250
MVADPDNPLV LDILTGSSTS YSFFPDKPIT QYPHAVGKNT LLIAGLQARN
260 270 280 290 300
NARVIFSGSL DFFSDAFFNS AVQKATPGSQ RYSQTGNYEL AVALSRWVFK
310 320 330 340 350
EEGVLRVGPV SHHRVGETAP PNAYTVTDLV EYSIVIEQLS NGRWVPFDGD
360 370 380 390 400
DIQLEFVRID PFVRTFLKKK GGKYSVQFKL PDVYGVFQFK VDYNRLGYTH
410 420 430 440 450
LYSSTQVSVR PLQHTQYERF IPSAYPYYAS AFSMMLGLFI FSIVFLHMKE
KEKSD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q2I3T2 | A0A3Q2I3T2_HORSE | Dolichyl-diphosphooligosaccharide--... | DDOST | 434 | Annotation score: |
Genome annotation databases
Ensembli | ENSECAT00000021027; ENSECAP00000017299; ENSECAG00000019546 |
Similar proteinsi
Cross-referencesi
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PaxDbi | F7CBX0 |
PRIDEi | F7CBX0 |
Genome annotation databases
Ensembli | ENSECAT00000021027; ENSECAP00000017299; ENSECAG00000019546 |
Organism-specific databases
VGNCi | VGNC:17080, DDOST |
Phylogenomic databases
GeneTreei | ENSGT00390000017294 |
HOGENOMi | CLU_031804_0_0_1 |
InParanoidi | F7CBX0 |
TreeFami | TF314821 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Gene expression databases
Bgeei | ENSECAG00000019546, Expressed in trophoblast and 22 other tissues |
ExpressionAtlasi | F7CBX0, baseline |
Family and domain databases
InterProi | View protein in InterPro IPR005013, DDOST_48_kDa_subunit |
PANTHERi | PTHR10830, PTHR10830, 1 hit |
Pfami | View protein in Pfam PF03345, DDOST_48kD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F7CBX0_HORSE | |
Accessioni | F7CBX0Primary (citable) accession number: F7CBX0 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 27, 2011 |
Last sequence update: | December 11, 2019 | |
Last modified: | August 12, 2020 | |
This is version 50 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |