UniProtKB - F7BJB9 (MORC3_MOUSE)
Protein
MORC family CW-type zinc finger protein 3
Gene
Morc3
Organism
Mus musculus (Mouse)
Status
Functioni
Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism (By similarity). Sumoylated MORC3-NBs can also associate with PML-NBs (By similarity). Recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity (PubMed:17332504). Binds RNA in vitro (By similarity). Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3 (By similarity). The order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0 (By similarity).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 404 – 454 | CW-typePROSITE-ProRule annotationAdd BLAST | 51 |
GO - Molecular functioni
- methylated histone binding Source: UniProtKB
- RNA binding Source: UniProtKB-KW
- zinc ion binding Source: InterPro
GO - Biological processi
- cell aging Source: MGI
- maintenance of protein location in nucleus Source: MGI
- negative regulation of fibroblast proliferation Source: MGI
- peptidyl-serine phosphorylation Source: MGI
- post-embryonic development Source: MGI
- protein phosphorylation Source: MGI
- protein stabilization Source: MGI
Keywordsi
Molecular function | RNA-binding |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: MORC family CW-type zinc finger protein 3By similarityAlternative name(s): Nuclear matrix protein 2By similarity Zinc finger CW-type coiled-coil domain protein 3By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2136841, Morc3 |
Subcellular locationi
Nucleus
- nucleoplasm 1 Publication
- Nucleus matrix By similarity
- PML body 1 Publication
Note: Also found in PML-independent nuclear bodies. Localization to nuclear bodies is ATP-dependent.By similarity
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PML body Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Lethality occurs at birth or within a day thereafter, for unknown reasons. Embryos at 14.5 dpc are morphologically indistinguishable from wild type.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000435141 | 1 – 942 | MORC family CW-type zinc finger protein 3Add BLAST | 942 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 191 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 205 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 280 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 293 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 517 | PhosphoserineBy similarity | 1 | |
Modified residuei | 543 | PhosphoserineBy similarity | 1 | |
Cross-linki | 558 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 563 | PhosphoserineCombined sources | 1 | |
Cross-linki | 604 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 604 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 657 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 657 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 658 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 658 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 743 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 743 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 768 | PhosphoserineBy similarity | 1 | |
Cross-linki | 797 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 797 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity |
Post-translational modificationi
Sumoylation is involved in interaction with PML and localization to PML nuclear bodies.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | F7BJB9 |
jPOSTi | F7BJB9 |
MaxQBi | F7BJB9 |
PaxDbi | F7BJB9 |
PRIDEi | F7BJB9 |
PTM databases
iPTMneti | F7BJB9 |
PhosphoSitePlusi | F7BJB9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000039456, Expressed in lymph node and 295 other tissues |
ExpressionAtlasi | F7BJB9, baseline and differential |
Genevisiblei | Q6A0C2, MM |
Interactioni
Subunit structurei
Homodimer. The sumoylated form interacts with PML (via SUMO-interacting motif).
Interacts with PT53.
1 PublicationGO - Molecular functioni
- methylated histone binding Source: UniProtKB
Protein-protein interaction databases
IntActi | F7BJB9, 3 interactors |
STRINGi | 10090.ENSMUSP00000040152 |
Miscellaneous databases
RNActi | F7BJB9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | F7BJB9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 326 – 353 | Nuclear matrix bindingBy similarityAdd BLAST | 28 | |
Regioni | 503 – 594 | RNA bindingBy similarityAdd BLAST | 92 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 696 – 874 | Sequence analysisAdd BLAST | 179 |
Domaini
The CW-TYPE zinc finger mediates its binding to trimethylated histone H3K4me3.By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 404 – 454 | CW-typePROSITE-ProRule annotationAdd BLAST | 51 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1845, Eukaryota |
GeneTreei | ENSGT00940000160495 |
InParanoidi | F7BJB9 |
OrthoDBi | 193855at2759 |
TreeFami | TF329118 |
Family and domain databases
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR036890, HATPase_C_sf IPR041006, Morc_S5 IPR011124, Znf_CW |
Pfami | View protein in Pfam PF17942, Morc6_S5, 1 hit PF07496, zf-CW, 1 hit |
SUPFAMi | SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS51050, ZF_CW, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
F7BJB9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAQPPTGIR LSALCPKFLH TNSTSHTWPF SAVAELIDNA YDPDVNAKQI
60 70 80 90 100
WIDKTVISDH ICLTFTDNGN GMTADKLHKM LSFGFSDKVT MNGHVPVGLY
110 120 130 140 150
GNGFKSGSMR LGKDAMVFTK NGETMSVGFL SQTYLEVIKA EHVVVPIVTF
160 170 180 190 200
NKHRQMINLT ESKASLAAIL EHSLFSTEQK LLAELNAIMG KKGTRIIIWN
210 220 230 240 250
LRSYKNATEF DFEKDKYDIR IPEDLDETAG RKGYKKQERM DQIAPESDYS
260 270 280 290 300
LRAYCSILYL KPRMQIIIRG QKVKTQLVSK SLAYIERDVY RPKFLTRTVR
310 320 330 340 350
ITFGFNCRNK DHYGIMMYHK NRLIKAYEKV GCQLKANNMG VGVVGIIECN
360 370 380 390 400
FLKPTHNKQD FDYTNEYRLT ILALGEKLND YWNEMKVKKN AEYPVNLPVE
410 420 430 440 450
DIQKRPDQTW VQCDACLKWR KLPDGIDQLP EKWYCSNNPD PQFRNCEVPE
460 470 480 490 500
EPEDEDLVHP TYEKTYKKTS KERFRIRQPE ILPRILPQIN PELLYQTSVS
510 520 530 540 550
SQSFSPVKES VPRPHLSEVT SPFAARIINL NLASPASEPE NSSMKRKLGV
560 570 580 590 600
HSSILNAKTR RLSNPPVENS SYKNDDDEDV IILEENSTPK PAVDLEVKSD
610 620 630 640 650
IEVKSEQSHT EQSGIHVDLV SSPKPCVQAS STSTSTSRSD PGITVSTQTD
660 670 680 690 700
APGLTVKKEE SMEEDMGVRN GTATLSCVGT EAKVQETSAE SVDATSHQLQ
710 720 730 740 750
ELRSELLVVT QERDDYKRQC QMFTDQIQVL QQRLLEMNDK CVKKEKCHQS
760 770 780 790 800
TETDAVFLLD SVNGQAESLD HLGSQYQQAL QEIERLKRQC SALQQVKSEC
810 820 830 840 850
SQASCTESKS EVDEMAVQLD DVFRQLDKCT IERDQYKNEV QLLEIEKSHI
860 870 880 890 900
HSQCEELQTE VEQLKSTGQQ AAADGSTASN AEEPVSYVDG ESLKLRSLRV
910 920 930 940
NVGQLLAMIV PDLDLQQVNY DVDVVDEILG QVVEQMSEIS ST
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YU83 | A0A0J9YU83_MOUSE | MORC family CW-type zinc finger pro... | Morc3 | 397 | Annotation score: | ||
A0A338P7B1 | A0A338P7B1_MOUSE | MORC family CW-type zinc finger pro... | Morc3 | 58 | Annotation score: | ||
A0A0J9YUV3 | A0A0J9YUV3_MOUSE | MORC family CW-type zinc finger pro... | Morc3 | 92 | Annotation score: |
Sequence cautioni
The sequence BAD32174 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 134 | Y → H in AAH98072 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 679 | G → S in AAI45706 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172896 mRNA Translation: BAD32174.1 Different initiation. AC168220 Genomic DNA No translation available. BC098072 mRNA Translation: AAH98072.1 BC145705 mRNA Translation: AAI45706.1 BC026506 mRNA Translation: AAH26506.1 |
CCDSi | CCDS79490.1 |
RefSeqi | NP_001038994.2, NM_001045529.3 |
Genome annotation databases
Ensembli | ENSMUST00000044068; ENSMUSP00000040152; ENSMUSG00000039456 ENSMUST00000202261; ENSMUSP00000144369; ENSMUSG00000039456 |
GeneIDi | 338467 |
KEGGi | mmu:338467 |
UCSCi | uc008aaa.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK172896 mRNA Translation: BAD32174.1 Different initiation. AC168220 Genomic DNA No translation available. BC098072 mRNA Translation: AAH98072.1 BC145705 mRNA Translation: AAI45706.1 BC026506 mRNA Translation: AAH26506.1 |
CCDSi | CCDS79490.1 |
RefSeqi | NP_001038994.2, NM_001045529.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IX1 | X-ray | 2.60 | A/B | 7-456 | [»] | |
5IX2 | X-ray | 2.90 | A/B | 7-456 | [»] | |
SMRi | F7BJB9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | F7BJB9, 3 interactors |
STRINGi | 10090.ENSMUSP00000040152 |
PTM databases
iPTMneti | F7BJB9 |
PhosphoSitePlusi | F7BJB9 |
Proteomic databases
EPDi | F7BJB9 |
jPOSTi | F7BJB9 |
MaxQBi | F7BJB9 |
PaxDbi | F7BJB9 |
PRIDEi | F7BJB9 |
Protocols and materials databases
Antibodypediai | 5016, 86 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044068; ENSMUSP00000040152; ENSMUSG00000039456 ENSMUST00000202261; ENSMUSP00000144369; ENSMUSG00000039456 |
GeneIDi | 338467 |
KEGGi | mmu:338467 |
UCSCi | uc008aaa.2, mouse |
Organism-specific databases
CTDi | 23515 |
MGIi | MGI:2136841, Morc3 |
Phylogenomic databases
eggNOGi | KOG1845, Eukaryota |
GeneTreei | ENSGT00940000160495 |
InParanoidi | F7BJB9 |
OrthoDBi | 193855at2759 |
TreeFami | TF329118 |
Miscellaneous databases
BioGRID-ORCSi | 338467, 1 hit in 13 CRISPR screens |
ChiTaRSi | Morc3, mouse |
PROi | PR:F7BJB9 |
RNActi | F7BJB9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039456, Expressed in lymph node and 295 other tissues |
ExpressionAtlasi | F7BJB9, baseline and differential |
Genevisiblei | Q6A0C2, MM |
Family and domain databases
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR036890, HATPase_C_sf IPR041006, Morc_S5 IPR011124, Znf_CW |
Pfami | View protein in Pfam PF17942, Morc6_S5, 1 hit PF07496, zf-CW, 1 hit |
SUPFAMi | SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS51050, ZF_CW, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MORC3_MOUSE | |
Accessioni | F7BJB9Primary (citable) accession number: F7BJB9 Secondary accession number(s): A6H605 Q8R0R0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 20, 2016 |
Last sequence update: | October 3, 2012 | |
Last modified: | December 2, 2020 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references