UniProtKB - F7AX17 (F7AX17_HORSE)
Protein
Abasic site processing protein HMCES
Gene
LOC100052108
Organism
Equus caballus (Horse)
Status
Functioni
Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then degraded by the proteasome. Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks. Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction.UniRule annotation
GO - Molecular functioni
- peptidase activity Source: UniProtKB-KW
- single-stranded DNA binding Source: InterPro
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB-KW
- protein-DNA covalent cross-linking Source: InterPro
Keywordsi
Molecular function | DNA-bindingARBA annotation, Hydrolase, ProteaseUniRule annotationARBA annotation |
Biological process | DNA damageARBA annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Abasic site processing protein HMCESUniRule annotationARBA annotation (EC:3.4.-.-UniRule annotation)Short name: ES cell-specific 5hmC-binding proteinUniRule annotation Alternative name(s): Embryonic stem cell-specific 5-hydroxymethylcytosine-binding proteinUniRule annotationARBA annotation Peptidase HMCESUniRule annotationARBA annotation SRAP domain-containing protein 1UniRule annotationARBA annotation |
Gene namesi | Name:LOC100052108Imported |
Organismi | Equus caballus (Horse)Imported |
Taxonomic identifieri | 9796 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › |
Proteomesi |
|
PTM / Processingi
Keywords - PTMi
Covalent protein-DNA linkageARBA annotationProteomic databases
PaxDbi | F7AX17 |
PRIDEi | F7AX17 |
Expressioni
Gene expression databases
Bgeei | ENSECAG00000022488, Expressed in blood and 22 other tissues |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 294 – 324 | DisorderedSequence analysisAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 308 – 324 | PolarSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00390000018439 |
HOGENOMi | CLU_035990_1_0_1 |
InParanoidi | F7AX17 |
TreeFami | TF324343 |
Family and domain databases
Gene3Di | 3.90.1680.10, 1 hit |
InterProi | View protein in InterPro IPR003738, SRAP IPR036590, SRAP-like |
PANTHERi | PTHR13604, PTHR13604, 1 hit |
Pfami | View protein in Pfam PF02586, SRAP, 1 hit |
SUPFAMi | SSF143081, SSF143081, 1 hit |
i Sequence
Sequence statusi: Complete.
F7AX17-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCGRTSCHLP RDVLTRACAY RNRQGERRLP EWRDADKYCP SYNKSPRSSS
60 70 80 90 100
PVLLSRLHFE KGTDSSERII VPMRWGLVPS WFKENDPSKL PFNITNCRSD
110 120 130 140 150
TIMEKRSFNV PLGKGRRCVV LADGFYEWKR CRGTYDRQPY FIYFPQTKSE
160 170 180 190 200
KLGSIGAADS PEDWNKVWDN WRLLTMAGIF DCWEPLQGGD HLYSYTIITV
210 220 230 240 250
DACKVLNDVH QRMPAILDGE EAVSKWLDFG EVSTQEALKL IHPTENITFH
260 270 280 290 300
PVSSVVNSSR NDSVECLAPI DLSVQKELKA KGCSQKMLQW LATKSPKKED
310 320 330 340 350
PKTPQKTESD VRQFLPKSPS SVKRSSAVLL EQWLKQEKEE EPVTKQLHSE
Sequence databases
RefSeqi | XP_001495096.1, XM_001495046.3 XP_005596276.1, XM_005596219.2 XP_014588941.1, XM_014733455.1 |
Genome annotation databases
Ensembli | ENSECAT00000024089; ENSECAP00000019995; ENSECAG00000022488 |
GeneIDi | 100052108 |
KEGGi | ecb:100052108 |
Similar proteinsi
Cross-referencesi
Sequence databases
RefSeqi | XP_001495096.1, XM_001495046.3 XP_005596276.1, XM_005596219.2 XP_014588941.1, XM_014733455.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PaxDbi | F7AX17 |
PRIDEi | F7AX17 |
Genome annotation databases
Ensembli | ENSECAT00000024089; ENSECAP00000019995; ENSECAG00000022488 |
GeneIDi | 100052108 |
KEGGi | ecb:100052108 |
Phylogenomic databases
GeneTreei | ENSGT00390000018439 |
HOGENOMi | CLU_035990_1_0_1 |
InParanoidi | F7AX17 |
TreeFami | TF324343 |
Gene expression databases
Bgeei | ENSECAG00000022488, Expressed in blood and 22 other tissues |
Family and domain databases
Gene3Di | 3.90.1680.10, 1 hit |
InterProi | View protein in InterPro IPR003738, SRAP IPR036590, SRAP-like |
PANTHERi | PTHR13604, PTHR13604, 1 hit |
Pfami | View protein in Pfam PF02586, SRAP, 1 hit |
SUPFAMi | SSF143081, SSF143081, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F7AX17_HORSE | |
Accessioni | F7AX17Primary (citable) accession number: F7AX17 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 27, 2011 |
Last sequence update: | April 10, 2019 | |
Last modified: | October 7, 2020 | |
This is version 42 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |