UniProtKB - F6VAN0 (ATF6A_MOUSE)
Cyclic AMP-dependent transcription factor ATF-6 alpha
Atf6
Functioni
Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane. Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR).
By similarityTranscription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
By similarityGO - Molecular functioni
- cAMP response element binding Source: InterPro
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- identical protein binding Source: MGI
- protein heterodimerization activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: GO_Central
- eye development Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of ATF6-mediated unfolded protein response Source: MGI
- positive regulation of autophagy Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- visual perception Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Transcription, Transcription regulation, Unfolded protein response |
Enzyme and pathway databases
Reactomei | R-MMU-381033, ATF6 (ATF6-alpha) activates chaperones |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic AMP-dependent transcription factor ATF-6 alphaShort name: cAMP-dependent transcription factor ATF-6 alpha Alternative name(s): Activating transcription factor 6 alpha Short name: ATF6-alpha Cleaved into the following chain: |
Gene namesi | Name:Atf6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926157, Atf6 |
VEuPathDBi | HostDB:ENSMUSG00000026663 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Single-pass type II membrane protein Sequence analysis
Golgi apparatus
- Golgi apparatus membrane By similarity; Single-pass type II membrane protein Sequence analysis
Note: Translocates from the endoplasmic reticulum to the Golgi, where it is processed.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- integral component of endoplasmic reticulum membrane Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: Ensembl
Other locations
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 377 | CytoplasmicSequence analysisAdd BLAST | 377 | |
Transmembranei | 378 – 398 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 399 – 656 | LumenalSequence analysisAdd BLAST | 258 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424336 | 1 – 656 | Cyclic AMP-dependent transcription factor ATF-6 alphaAdd BLAST | 656 | |
ChainiPRO_0000424337 | 1 – ? | Processed cyclic AMP-dependent transcription factor ATF-6 alphaBy similarity |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 75 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Glycosylationi | 459 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 570 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 629 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 406 – 407 | Cleavage; by MBTPS1By similarity | 2 |
Keywords - PTMi
Glycoprotein, Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | F6VAN0 |
PaxDbi | F6VAN0 |
PeptideAtlasi | F6VAN0 |
ProteomicsDBi | 277213 |
PTM databases
GlyConnecti | 2243, 2 N-Linked glycans (1 site) |
GlyGeni | F6VAN0, 3 sites, 2 N-linked glycans (1 site) |
iPTMneti | F6VAN0 |
PhosphoSitePlusi | F6VAN0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026663, Expressed in submandibular gland and 289 other tissues |
Genevisiblei | F6VAN0, MM |
Interactioni
Subunit structurei
Interacts with XBP1 isoform 2; the interaction occurs in a ER stress-dependent manner.
Interacts with LACC1.
By similarityHomodimer and heterodimer with ATF6-beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC).
Interacts also with the transcription factors GTF2I, YY1 and SRF.
By similarityBinary interactionsi
GO - Molecular functioni
- identical protein binding Source: MGI
- protein heterodimerization activity Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230540, 4 interactors |
DIPi | DIP-59768N |
IntActi | F6VAN0, 4 interactors |
STRINGi | 10090.ENSMUSP00000027974 |
Miscellaneous databases
RNActi | F6VAN0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 293 – 356 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 137 | Transcription activationCuratedAdd BLAST | 137 | |
Regioni | 75 – 169 | DisorderedSequence analysisAdd BLAST | 95 | |
Regioni | 295 – 326 | Basic motifAdd BLAST | 32 | |
Regioni | 335 – 342 | Leucine-zipper | 8 | |
Regioni | 391 – 429 | DisorderedSequence analysisAdd BLAST | 39 | |
Regioni | 455 – 575 | Interaction with THBS41 PublicationAdd BLAST | 121 | |
Regioni | 632 – 656 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 79 – 132 | Polar residuesSequence analysisAdd BLAST | 54 | |
Compositional biasi | 140 – 156 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 405 – 425 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4343, Eukaryota |
GeneTreei | ENSGT00940000159221 |
HOGENOMi | CLU_026136_1_0_1 |
InParanoidi | F6VAN0 |
OMAi | NQFCTVK |
OrthoDBi | 696548at2759 |
PhylomeDBi | F6VAN0 |
TreeFami | TF316079 |
Family and domain databases
InterProi | View protein in InterPro IPR029801, ATF6A IPR004827, bZIP |
PANTHERi | PTHR46164:SF1, PTHR46164:SF1, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MESPFSPVLP HGPDEDWEST LFAELGYFTD TDDVHFDAAH EAYENNFDHL
60 70 80 90 100
NFDLDLMPWE SDLWSPGSHF CSDMKAEPQP LSPASSSCSI SSPRSTDSCS
110 120 130 140 150
STQHVPEELD LLSSSQSPLS LYGDSCNSPS SVEPLKEEKP VTGPGNKTEH
160 170 180 190 200
GLTPKKKIQM SSKPSVQPKP LLLPAAPKTQ TNASVPAKAI IIQTLPALMP
210 220 230 240 250
LAKQQSIISI QPAPTKGQTV LLSQPTVVQL QSPAVLSSAQ PVLAVTGGAA
260 270 280 290 300
QLPNHVVNVL PAPVVSSPVN GKLSVTKPVL QSATRSMGSD IAVLRRQQRM
310 320 330 340 350
IKNRESACQS RKKKKEYMLG LEARLKAALS ENEQLKKENG SLKRQLDEVV
360 370 380 390 400
SENQRLKVPS PKRRAVCVMI VLAFIMLNYG PMSMLEQESR RVKPSVSPAN
410 420 430 440 450
QRRHLLEFSA KEVKDTSDGD NQKDSYSYDH SVSNDKALMV LSEEPLLYMP
460 470 480 490 500
PPPCQPLINT TESLRLNHEL RGWVHRHEVE RTKSRRMTNS QQKARILQGA
510 520 530 540 550
LEQGSNSQLM AVQYTETTSI SRNSGSELQV YYASPGSYQG FFDAIRRRGD
560 570 580 590 600
TFYVVSFRRD HLLLPATTHN KTTRPKMSIV LPAININDNV INGQDYEVMM
610 620 630 640 650
QIDCQVMDTR ILHIKSSSVP PYLRDHQRNQ TSTFFGSPPT TTETTHVVST
IPESLQ
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 141 | V → I in AAI41029 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 237 | S → P in AAI41029 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 441 | L → P in BAC29389 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK036335 mRNA Translation: BAC29389.1 Different initiation. AC113490 Genomic DNA No translation available. AC125021 Genomic DNA No translation available. BC141028 mRNA Translation: AAI41029.1 |
CCDSi | CCDS56653.1 |
RefSeqi | NP_001074773.1, NM_001081304.1 |
Genome annotation databases
Ensembli | ENSMUST00000027974; ENSMUSP00000027974; ENSMUSG00000026663 |
GeneIDi | 226641 |
KEGGi | mmu:226641 |
UCSCi | uc007dmj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK036335 mRNA Translation: BAC29389.1 Different initiation. AC113490 Genomic DNA No translation available. AC125021 Genomic DNA No translation available. BC141028 mRNA Translation: AAI41029.1 |
CCDSi | CCDS56653.1 |
RefSeqi | NP_001074773.1, NM_001081304.1 |
3D structure databases
SMRi | F6VAN0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230540, 4 interactors |
DIPi | DIP-59768N |
IntActi | F6VAN0, 4 interactors |
STRINGi | 10090.ENSMUSP00000027974 |
PTM databases
GlyConnecti | 2243, 2 N-Linked glycans (1 site) |
GlyGeni | F6VAN0, 3 sites, 2 N-linked glycans (1 site) |
iPTMneti | F6VAN0 |
PhosphoSitePlusi | F6VAN0 |
Proteomic databases
MaxQBi | F6VAN0 |
PaxDbi | F6VAN0 |
PeptideAtlasi | F6VAN0 |
ProteomicsDBi | 277213 |
Protocols and materials databases
Antibodypediai | 20515, 634 antibodies from 46 providers |
DNASUi | 226641 |
Genome annotation databases
Ensembli | ENSMUST00000027974; ENSMUSP00000027974; ENSMUSG00000026663 |
GeneIDi | 226641 |
KEGGi | mmu:226641 |
UCSCi | uc007dmj.1, mouse |
Organism-specific databases
CTDi | 22926 |
MGIi | MGI:1926157, Atf6 |
VEuPathDBi | HostDB:ENSMUSG00000026663 |
Phylogenomic databases
eggNOGi | KOG4343, Eukaryota |
GeneTreei | ENSGT00940000159221 |
HOGENOMi | CLU_026136_1_0_1 |
InParanoidi | F6VAN0 |
OMAi | NQFCTVK |
OrthoDBi | 696548at2759 |
PhylomeDBi | F6VAN0 |
TreeFami | TF316079 |
Enzyme and pathway databases
Reactomei | R-MMU-381033, ATF6 (ATF6-alpha) activates chaperones |
Miscellaneous databases
BioGRID-ORCSi | 226641, 0 hits in 63 CRISPR screens |
ChiTaRSi | Atf6, mouse |
PROi | PR:F6VAN0 |
RNActi | F6VAN0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026663, Expressed in submandibular gland and 289 other tissues |
Genevisiblei | F6VAN0, MM |
Family and domain databases
InterProi | View protein in InterPro IPR029801, ATF6A IPR004827, bZIP |
PANTHERi | PTHR46164:SF1, PTHR46164:SF1, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ATF6A_MOUSE | |
Accessioni | F6VAN0Primary (citable) accession number: F6VAN0 Secondary accession number(s): B2RU98, Q8BZ84 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2013 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 23, 2022 | |
This is version 86 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families