UniProtKB - F6REQ2 (F6REQ2_HORSE)
Protein
Nitric oxide synthase
Gene
NOS3
Organism
Equus caballus (Horse)
Status
Functioni
Produces nitric oxide (NO).UniRule annotation
Catalytic activityi
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxideUniRule annotationARBA annotationEC:1.14.13.39UniRule annotationARBA annotation
Cofactori
Protein has several cofactor binding sites:- (6R)-L-erythro-5,6,7,8-tetrahydrobiopterinARBA annotation
- hemeUniRule annotationARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 185 | Iron (heme axial ligand)UniRule annotation | 1 |
GO - Molecular functioni
- actin monomer binding Source: Ensembl
- arginine binding Source: Ensembl
- calmodulin binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: GO_Central
- FMN binding Source: GO_Central
- heme binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- NADPH-hemoprotein reductase activity Source: GO_Central
- nitric-oxide synthase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: GO_Central
- tetrahydrobiopterin binding Source: Ensembl
GO - Biological processi
- arginine catabolic process Source: GO_Central
- endothelial cell migration Source: Ensembl
- negative regulation of blood pressure Source: GO_Central
- nitric oxide biosynthetic process Source: GO_Central
- nitric oxide mediated signal transduction Source: GO_Central
- positive regulation of angiogenesis Source: Ensembl
- positive regulation of blood vessel endothelial cell migration Source: Ensembl
- positive regulation of guanylate cyclase activity Source: GO_Central
- regulation of systemic arterial blood pressure by endothelin Source: Ensembl
- removal of superoxide radicals Source: Ensembl
- response to fluid shear stress Source: Ensembl
- response to hormone Source: GO_Central
- response to lipopolysaccharide Source: GO_Central
Keywordsi
Molecular function | Calmodulin-bindingUniRule annotationARBA annotation, OxidoreductaseUniRule annotationARBA annotation |
Ligand | FADUniRule annotationARBA annotation, Flavoprotein, FMNUniRule annotationARBA annotation, HemeUniRule annotationARBA annotation, Iron, Metal-binding, NADPUniRule annotationARBA annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NOS3Imported |
Organismi | Equus caballus (Horse)Imported |
Taxonomic identifieri | 9796 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:20815, NOS3 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: Ensembl
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- caveola Source: Ensembl
- plasma membrane Source: GO_Central
Other locations
- vesicle membrane Source: GO_Central
Expressioni
Gene expression databases
Bgeei | ENSECAG00000024562, Expressed in synovial membrane of synovial joint and 20 other tissues |
ExpressionAtlasi | F6REQ2, baseline |
Interactioni
GO - Molecular functioni
- actin monomer binding Source: Ensembl
- calmodulin binding Source: UniProtKB-KW
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 521 – 704 | Flavodoxin-likeInterPro annotationAdd BLAST | 184 | |
Domaini | 757 – 1003 | FAD-binding FR-typeInterPro annotationAdd BLAST | 247 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 72 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 820 – 849 | DisorderedSequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 37 – 66 | Pro-richSequence analysisAdd BLAST | 30 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00940000161389 |
InParanoidi | F6REQ2 |
TreeFami | TF324410 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
F6REQ2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNLKSVGQE PGPPCGLGLG LGLGLCGKQG PASPASEPSR APVPAPAPAP
60 70 80 90 100
LPAPDHSPPL ARPPEGPKFP RVKNWEVGSI TYDTLCAQSQ QDGPCTPRRC
110 120 130 140 150
LGSLVFPRKL QSRPSQGPPP PEQLLSQARD FINQYYSSIK RSGSQAHEQR
160 170 180 190 200
LQEVEAEVAA TGTYQLRESE LVFGAKQAWR NAPRCVGRIQ WGKLQVFDAR
210 220 230 240 250
DCSSAQEMFT CICNHIKYAT NRGNLRSAIT VFPQRTPGRG DFRIWNSQLV
260 270 280 290 300
RYAGYRQQDG SVRGDPANVE ITELCVQHGW TPGNGRFDVL PLLLQAPDEP
310 320 330 340 350
PELFTLPPEL VLEVPLEHPT LEWFASLGLR WYALPAVSNM LLEIGGLEFP
360 370 380 390 400
AAPFSGWYMS TEIGTRNLCD PHRYNILEDV AVCMDLDTRT TSSLWKDKAA
410 420 430 440 450
VEINLAVLHS YQLAKVTIVD HHAATASFMK HLENEQKARG GCPADWAWIV
460 470 480 490 500
PPISGSLTPV FHQEMVNYVL SPAFRYQPDP WKGSAVKGTG LARKKTFKEV
510 520 530 540 550
ANAVKISASL MGTVMAKRVK ATILYGSETG RAQGYAQQLG RLFRKAFDPR
560 570 580 590 600
VLCMDEYDVV SLEHETLVLV VTSTFGNGDP PENGESFAAA LMEMSGPYNS
610 620 630 640 650
SPRPEQHKSY KIRFNSVSCS DPLVSSWRRK RKESSNTDSA GALGTLRFCV
660 670 680 690 700
FGLGSRAYPH FCAFARAVDT RLEELGGERL LQLGQGDELC GQEEAFRGWA
710 720 730 740 750
QAAFQASCET FCVGEDAKAA ARDIFSPKRS WKRQRYRLST QAEGLQLLPG
760 770 780 790 800
LIHVHRRKMF QATVLSVENL QSSKSTRATI LVRLDTGGQE GLQYQPGDHI
810 820 830 840 850
GVCPPNRPGL VEALLSRVED PPPAGEPVAV EQLEKGSPGG PPPSWVRDPR
860 870 880 890 900
LPPCTLRQAL TFFLDITSPP SPQLLRLLST LAEEPSEQQE LETLSQDPRR
910 920 930 940 950
YEEWKWFRCP TLLEVLEQFP SVALPAALLL TQLPLLQPRY YSVSSAPSTH
960 970 980 990 1000
PGEIHLTVAV LAYRTQDGLG PLHYGVCSTW LSQLKAGDLV PCFIRGAPSF
1010 1020 1030 1040 1050
RLPPDPSLPC ILVGPGTGIA PFRGFWQERL HDIESKGLQP APMTLVFGCR
1060 1070 1080 1090 1100
CSQLDHLYRD EVQDAQQRGV FGRVLTAFSR EPDSPKTYVQ DILRTELAAE
1110 1120 1130 1140 1150
VHRVLCLDRG HMFVCGDVTM ATSVLQTVQR ILATEGDMEL DEAGDVIGVL
1160 1170 1180 1190 1200
RVRRSRSRGE AGPCRGRGPL RYRSPYCGSD PLRSFSAGSA TLSRGHFRAH
1210 1220 1230 1240 1250
AAHPGGDKPH THPEPFLARA ASAGHSALGV RPARPRHLWS LRVLLLSTPV
1260
PGEGLSVHPG SAAPLSA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6REP3 | F6REP3_HORSE | Nitric oxide synthase | NOS3 | 1,204 | Annotation score: |
Genome annotation databases
Ensembli | ENSECAT00000026728; ENSECAP00000022325; ENSECAG00000024562 |
Similar proteinsi
Cross-referencesi
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Proteomic databases
PaxDbi | F6REQ2 |
Genome annotation databases
Ensembli | ENSECAT00000026728; ENSECAP00000022325; ENSECAG00000024562 |
Organism-specific databases
VGNCi | VGNC:20815, NOS3 |
Phylogenomic databases
GeneTreei | ENSGT00940000161389 |
InParanoidi | F6REQ2 |
TreeFami | TF324410 |
Gene expression databases
Bgeei | ENSECAG00000024562, Expressed in synovial membrane of synovial joint and 20 other tissues |
ExpressionAtlasi | F6REQ2, baseline |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F6REQ2_HORSE | |
Accessioni | F6REQ2Primary (citable) accession number: F6REQ2 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | July 27, 2011 |
Last sequence update: | April 10, 2019 | |
Last modified: | August 12, 2020 | |
This is version 66 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |