UniProtKB - F6BCS4 (THI4_METIK)
Protein
Thiamine thiazole synthase
Gene
thi4
Organism
Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5)
Status
Functioni
Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation1 Publication
Catalytic activityi
- glycine + hydrogen sulfide + NAD+ = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H+ + 3 H2O + nicotinamide1 PublicationEC:2.4.2.591 Publication
Cofactori
Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 40 | NAD1 Publication | 1 | |
Binding sitei | 67 | NAD; via amide nitrogen and carbonyl oxygen1 Publication | 1 | |
Binding sitei | 133 | NAD; via amide nitrogen and carbonyl oxygen1 Publication | 1 | |
Metal bindingi | 161 | Iron; shared with adjacent protomer1 Publication | 1 | |
Metal bindingi | 176 | Iron1 Publication | 1 | |
Binding sitei | 179 | NAD; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 226 | NAD; via amide nitrogen1 Publication | 1 | |
Binding sitei | 236 | Glycine1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 59 – 60 | NAD1 Publication | 2 | |
Nucleotide bindingi | 159 – 161 | NAD; shared with adjacent protomerUniRule annotation | 3 |
GO - Molecular functioni
- iron ion binding Source: UniProtKB-UniRule
- transferase activity, transferring pentosyl groups Source: UniProtKB-UniRule
GO - Biological processi
- thiamine biosynthetic process Source: UniProtKB-UniRule
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
- thiazole biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Transferase |
Biological process | Thiamine biosynthesis |
Ligand | Iron, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | MIGN880724:G1GY0-742-MONOMER |
UniPathwayi | UPA00060 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:thi41 Publication Ordered Locus Names:Metig_0735Imported |
Organismi | Methanotorris igneus (strain DSM 5666 / JCM 11834 / Kol 5) |
Taxonomic identifieri | 880724 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanocaldococcaceae › Methanotorris › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000438857 | 1 – 263 | Thiamine thiazole synthaseAdd BLAST | 263 |
Interactioni
Subunit structurei
Homooctamer; tetramer of dimers.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | F6BCS4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the THI4 family.UniRule annotation
Phylogenomic databases
HOGENOMi | CLU_053727_2_0_2 |
OMAi | MWGGGMM |
OrthoDBi | 61905at2157 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00304, Thi4, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR002922, Thi4_fam IPR022828, Thi4_prok |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR00292, TIGR00292, 1 hit |
i Sequence
Sequence statusi: Complete.
F6BCS4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVRLRADEY ATTRAILKSA FDMWLDIIDV DVAIVGGGPS GLTAARYIAK
60 70 80 90 100
EGYKVVVLER HLAFGGGTWG GGMGFPYIVV EEPADEILRE VGVKLEKVEG
110 120 130 140 150
EDGLYTADSV EVPAKLAVGA IDAGAKVLTG IVVEDLVLRE NRVAGVVINS
160 170 180 190 200
YAIEKAGLHI DPITITAKYV VDATGHDASV VTTLSRKNPE LGLEVPGEKS
210 220 230 240 250
MWAEKGENAL LRNTREVYPG LFVCGMAANA VYAGHRMGAI FGGMYISGKK
260
CAEMIVEKLK NNE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002737 Genomic DNA Translation: AEF96285.1 |
RefSeqi | WP_013798888.1, NC_015562.1 |
Genome annotation databases
EnsemblBacteriai | AEF96285; AEF96285; Metig_0735 |
GeneIDi | 10643575 |
KEGGi | mig:Metig_0735 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP002737 Genomic DNA Translation: AEF96285.1 |
RefSeqi | WP_013798888.1, NC_015562.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4Y4N | X-ray | 2.10 | A/B/C/D/E/F/G/H | 1-263 | [»] | |
SMRi | F6BCS4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
EnsemblBacteriai | AEF96285; AEF96285; Metig_0735 |
GeneIDi | 10643575 |
KEGGi | mig:Metig_0735 |
Phylogenomic databases
HOGENOMi | CLU_053727_2_0_2 |
OMAi | MWGGGMM |
OrthoDBi | 61905at2157 |
Enzyme and pathway databases
UniPathwayi | UPA00060 |
BioCyci | MIGN880724:G1GY0-742-MONOMER |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
HAMAPi | MF_00304, Thi4, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR002922, Thi4_fam IPR022828, Thi4_prok |
SUPFAMi | SSF51905, SSF51905, 1 hit |
TIGRFAMsi | TIGR00292, TIGR00292, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THI4_METIK | |
Accessioni | F6BCS4Primary (citable) accession number: F6BCS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 15, 2017 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 54 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families