UniProtKB - F4KCC2 (PRT6_ARATH)
Protein
E3 ubiquitin-protein ligase PRT6
Gene
PRT6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Ubiquitin protein ligase which is a component of the N-end rule pathway with arginine specificity, and functions with the arginyltransferases ATE1 and ATE2. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (PubMed:17572409, PubMed:19255443, PubMed:19620738, PubMed:22020282). Does not participate in degradation of proteins with N-terminal Phe or Leu (PubMed:17572409). The N-end rule pathway regulates seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination (PubMed:19255443). The N-end rule pathway regulates various aspects of leaf and shoot development (PubMed:19620738). Involved in the ubiquitination and subsequent degradation of RAP2-12, an activator of hypoxic gene expression. The ubiquitination occurs after the N-arginylation of RAP2-12 by ATE1 or ATE2 under aerobic conditions (PubMed:22020282). The end-rule pathway plays a role in regulating the timing and amplitude of the immune response following infection with the bacterial pathogen Pseudomonas syringae pv tomato (PubMed:27173012, PubMed:30117535). Regulates the biosynthesis of plant-defense metabolites such as glucosinolates, and the biosynthesis and response to the phytohormone jasmonate (JA), which plays a key role in plant immunity (PubMed:27173012). Controls the expression of specific defense-response genes, activates the synthesis pathway for the phytoalexin camalexin, and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato (PubMed:30117535). Coordinates the mobilization of seed storage reserves and regulates the abundance and activities of several proteases following seed germination (PubMed:29168982).7 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 119 – 189 | UBR-typePROSITE-ProRule annotationAdd BLAST | 71 | |
Zinc fingeri | 1395 – 1440 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 46 |
GO - Molecular functioni
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activity Source: TAIR
- zinc ion binding Source: InterPro
GO - Biological processi
- defense response to bacterium Source: TAIR
- defense response to fungus Source: TAIR
- protein ubiquitination Source: GO_Central
- regulation of lipid catabolic process Source: TAIR
- regulation of seed germination Source: TAIR
- response to abscisic acid Source: TAIR
- ubiquitin-dependent protein catabolic process Source: TAIR
- ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Plant defense, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase PRT6Curated (EC:2.3.2.27)Alternative name(s): Protein GREENING AFTER EXTENDED DARKNESS 11 Publication Protein PROTEOLYSIS 61 Publication RING-type E3 ubiquitin transferase PRT6Curated |
Gene namesi | Name:PRT61 Publication Synonyms:CER31 Publication, GED11 Publication Ordered Locus Names:At5g02310/At5g02300Imported ORF Names:T1E22.70/T1E22.60Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G02310 |
TAIRi | locus:2180147, AT5G02310 |
Subcellular locationi
Other locations
- cytoplasm Source: GO_Central
- ubiquitin ligase complex Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000431720 | 1 – 2006 | E3 ubiquitin-protein ligase PRT6Add BLAST | 2006 |
Proteomic databases
PaxDbi | F4KCC2 |
PRIDEi | F4KCC2 |
ProteomicsDBi | 226480 |
PTM databases
iPTMneti | F4KCC2 |
Expressioni
Gene expression databases
ExpressionAtlasi | F4KCC2, baseline and differential |
Genevisiblei | F4KCC2, AT |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | F4KCC2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1627 – 1633 | Poly-SerSequence analysis | 7 | |
Compositional biasi | 1878 – 1923 | Cys-richPROSITE-ProRule annotationAdd BLAST | 46 |
Sequence similaritiesi
Belongs to the UBR1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 119 – 189 | UBR-typePROSITE-ProRule annotationAdd BLAST | 71 | |
Zinc fingeri | 1395 – 1440 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 46 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1139, Eukaryota KOG1140, Eukaryota |
HOGENOMi | CLU_001801_1_0_1 |
InParanoidi | F4KCC2 |
OrthoDBi | 81415at2759 |
Family and domain databases
Gene3Di | 1.10.10.2670, 1 hit |
InterProi | View protein in InterPro IPR042065, E3_ELL-like IPR044046, E3_ligase_UBR-like_C IPR039164, UBR1-like IPR036390, WH_DNA-bd_sf IPR003126, Znf_UBR |
PANTHERi | PTHR21497, PTHR21497, 1 hit |
Pfami | View protein in Pfam PF18995, PRT6_C, 1 hit PF02207, zf-UBR, 1 hit |
SMARTi | View protein in SMART SM00396, ZnF_UBR1, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51157, ZF_UBR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
F4KCC2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METNSSLFGL VSPSSHDLVI ERLASVGVPK KYRSKRGLVE FVRANPAKIS
60 70 80 90 100
ELVSALLPTD DDVKLGLKEA RERPRKSAVS PTMKKRFRES MNMLQWLMFQ
110 120 130 140 150
DEPDVSLRNL AKLNLDQRGV CGSVWGQNDI AYRCRTCEND PTCAICVPCF
160 170 180 190 200
QNGDHNSHDY SIIYTGGGCC DCGDETAWKP DGFCSNHKGS EQIRPLSENL
210 220 230 240 250
ANSVGPILDA LFTCWNNKLL SAESSGQKGA RSNDTLVILQ KMSNELTFIV
260 270 280 290 300
VEMLLEFSMS SESLLSFVSR RIISSSGLLS ILLKAERFLD QDVMKKLHDL
310 320 330 340 350
FLKLIGDPVF KCEFAKAFVS YYPVVISEVV KQGTDNAFKK YPLLSTFSVQ
360 370 380 390 400
ILTVPTLTPF LVKEMNLLAM LLGCLSDIFV SCSGEDGLLQ ATKLERLCET
410 420 430 440 450
SERVIGDLKF VMSHAIVSKY ATHEHRELSR SWLTLLTFAQ GMNPLKRETG
460 470 480 490 500
IPIDEENDYM HLFFVLGHSI AVIHSLLVNG TYSAASDEEI ENDRNAKEEF
510 520 530 540 550
DKCDGDGERY AKVGRLSHED SVCTAIVSSS SFDSSMASEV HKIDPFHALL
560 570 580 590 600
PSSAIYLIRE CLKVLETCLG NDEGISKFLC KLSSSSGRNI PESKMSWPRR
610 620 630 640 650
DLLNVETGGS VSSNLASSSR DPSTGLSPLC GDIQTNLSLD NVCGPYGVVQ
660 670 680 690 700
TDVTADSKRV SCNSADLTKN ASGLRILGLC DWPDIHYDVS SQAISVHLPL
710 720 730 740 750
HRLLSLLIQK ALRICYGESA SYNGVSISHE IPHADFFSSV IGDFHPCGFS
760 770 780 790 800
ALVMEHVLQI RVFCAQVIAG MWKKNGDSAL VSCEWYRSVR WSEQGLELDL
810 820 830 840 850
FLLQCCAALA PADSYVDKLL SRFGLSSYLS LNPDITNEYE PVLVQEMLGL
860 870 880 890 900
LIQILQERRF CGLSTAESLR REIIFKLATG DFTHSQLVKS LPRDLSKSDE
910 920 930 940 950
LQEVLDDVSV YCNPSGMNQG KYSLQSSCWK ELDLYHPRWQ SRDLQSAEER
960 970 980 990 1000
FSRYCGVSAL TTQLPRWRMI YPPLKGLARI GTCKATFQII SSALYYALQS
1010 1020 1030 1040 1050
GTSVKSRAPD GVLITALQLL SLSLDICTQQ RQSNSQDCCL ENSIPILELA
1060 1070 1080 1090 1100
GLEIIGIAQG TEKESLLSLL VSLMKTRMGD GRHQFPEPGS CNISSWIGNL
1110 1120 1130 1140 1150
LKKFSAIDSV CMNLLQSLAP EVVGQSGFDK VMSGSTSDEK RKAKAKERQA
1160 1170 1180 1190 1200
AIMAKMKAEQ SKFLSTLSSS MDDDDPRSEF ETSDSVMEHD SEIAVREVCS
1210 1220 1230 1240 1250
LCHDPDSKDP VSFLIFLQKS KLLSFVDRGP PSWDQCPQSE KKISVDGAPD
1260 1270 1280 1290 1300
LLRMNASSDS LRISSPLMLQ LSDDTISESA NMIESIKARL IGNGQTEKRS
1310 1320 1330 1340 1350
SDGRGKDESN MESLEIAMYQ TVRNKIENMI NQSLTRVDHQ PHEAENCSEK
1360 1370 1380 1390 1400
NSVGGPSTLQ GRFPDIRSRQ TSRRPDAGSD GFHPIDCDGV YLSSCGHAVH
1410 1420 1430 1440 1450
QSCLERYLKS LKERSGRRTV FEGAHIVDLK KKEFLCPVCR RLANSVLPEC
1460 1470 1480 1490 1500
PGDLCSVSKL QDSPRTKLRR KDALQPSLWL SEALCLLRSA AEVIEDGDRG
1510 1520 1530 1540 1550
KTVTPQGDGP RRKDLKSVSK MLWDFYFPKP EDKTLKRLWL PPQSIVMWDT
1560 1570 1580 1590 1600
LKYSLISMEI GTRFAKNSML PVYCIDSLYE ELKTSKGTIL SVLLRVVQSS
1610 1620 1630 1640 1650
RTKNTIHVRQ RFVGMKHLAE SICYGVSSSS SSSIFGSEGT TGSLKNIDLL
1660 1670 1680 1690 1700
WNRASDPVLA HDPFSSLMWA LFCLPFPFLT CEESLLSLVH IFHSVSLVQT
1710 1720 1730 1740 1750
VIAYCACRPS ELSELNFGEN LLNDISNALR ESGGWEYFRS NNMDLSCDIK
1760 1770 1780 1790 1800
DTIRKYSLPF LRRCALLWKL LKSTPRKLHE ESDMFDLPSD PTTDNMDFIY
1810 1820 1830 1840 1850
SPQSELNHVQ ELEKMFNIPP IDIILNDELL RSSTQIWLQH FQREYRVNRV
1860 1870 1880 1890 1900
KRSLCITPVV PFQLMKLPNL YQDLLQRCIK KRCVNCTKVI EEPVLCLLCG
1910 1920 1930 1940 1950
SLCSPIWSPC CRESGCPNHA ITCGAGTGVF LLIRRTTILL QRFARQSPWP
1960 1970 1980 1990 2000
SPYLDTFGEE DIDMIRGKRL YLNEERYAAL TYLVGSHGLD RSSKVLGQTT
IGAVLH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BFH3 | A0A1P8BFH3_ARATH | E3 ubiquitin-protein ligase | PRT6 proteolysis 6, At5g02300, At5g02310, T1E22.70, T1E22_70 | 1,924 | Annotation score: |
Sequence cautioni
The sequence CAB85535 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g02300 and At5g02310.Curated
The sequence CAB85536 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g02300 and At5g02310.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1341 | P → H in CAA65198 (PubMed:8811860).Curated | 1 | |
Sequence conflicti | 1430 | Missing in CAA65198 (PubMed:8811860).Curated | 1 | |
Sequence conflicti | 1838 | L → F in CAA65198 (PubMed:8811860).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL162874 Genomic DNA Translation: CAB85535.1 Sequence problems. AL162874 Genomic DNA Translation: CAB85536.1 Sequence problems. CP002688 Genomic DNA Translation: AED90457.1 CP002688 Genomic DNA Translation: ANM70335.1 X95962 Genomic DNA Translation: CAA65198.1 |
PIRi | T48251 T48252 |
RefSeqi | NP_001318457.1, NM_001342651.1 NP_195851.2, NM_120309.2 |
Genome annotation databases
EnsemblPlantsi | AT5G02310.1; AT5G02310.1; AT5G02310 AT5G02310.3; AT5G02310.3; AT5G02310 |
GeneIDi | 830916 |
Gramenei | AT5G02310.1; AT5G02310.1; AT5G02310 AT5G02310.3; AT5G02310.3; AT5G02310 |
KEGGi | ath:AT5G02310 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL162874 Genomic DNA Translation: CAB85535.1 Sequence problems. AL162874 Genomic DNA Translation: CAB85536.1 Sequence problems. CP002688 Genomic DNA Translation: AED90457.1 CP002688 Genomic DNA Translation: ANM70335.1 X95962 Genomic DNA Translation: CAA65198.1 |
PIRi | T48251 T48252 |
RefSeqi | NP_001318457.1, NM_001342651.1 NP_195851.2, NM_120309.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6LHN | X-ray | 2.50 | A | 119-189 | [»] | |
SMRi | F4KCC2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G02310.1 |
PTM databases
iPTMneti | F4KCC2 |
Proteomic databases
PaxDbi | F4KCC2 |
PRIDEi | F4KCC2 |
ProteomicsDBi | 226480 |
Genome annotation databases
EnsemblPlantsi | AT5G02310.1; AT5G02310.1; AT5G02310 AT5G02310.3; AT5G02310.3; AT5G02310 |
GeneIDi | 830916 |
Gramenei | AT5G02310.1; AT5G02310.1; AT5G02310 AT5G02310.3; AT5G02310.3; AT5G02310 |
KEGGi | ath:AT5G02310 |
Organism-specific databases
Araporti | AT5G02310 |
TAIRi | locus:2180147, AT5G02310 |
Phylogenomic databases
eggNOGi | KOG1139, Eukaryota KOG1140, Eukaryota |
HOGENOMi | CLU_001801_1_0_1 |
InParanoidi | F4KCC2 |
OrthoDBi | 81415at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:F4KCC2 |
Gene expression databases
ExpressionAtlasi | F4KCC2, baseline and differential |
Genevisiblei | F4KCC2, AT |
Family and domain databases
Gene3Di | 1.10.10.2670, 1 hit |
InterProi | View protein in InterPro IPR042065, E3_ELL-like IPR044046, E3_ligase_UBR-like_C IPR039164, UBR1-like IPR036390, WH_DNA-bd_sf IPR003126, Znf_UBR |
PANTHERi | PTHR21497, PTHR21497, 1 hit |
Pfami | View protein in Pfam PF18995, PRT6_C, 1 hit PF02207, zf-UBR, 1 hit |
SMARTi | View protein in SMART SM00396, ZnF_UBR1, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit |
PROSITEi | View protein in PROSITE PS51157, ZF_UBR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRT6_ARATH | |
Accessioni | F4KCC2Primary (citable) accession number: F4KCC2 Secondary accession number(s): Q96248, Q9LZ94, Q9LZ95 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 4, 2015 |
Last sequence update: | June 28, 2011 | |
Last modified: | December 2, 2020 | |
This is version 68 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families