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UniProtKB - F4JKK0 (SUD1_ARATH)
Protein
Probable E3 ubiquitin ligase SUD1
Gene
SUD1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Probable E3 ubiquitin ligase acting as a positive post-transcriptional regulator of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity. Might be involved in the quality control that degrades misfolded proteins (By similarity).
By similarity2 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 68 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 71 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 85 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 87 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 95 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 98 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 111 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 114 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 60 – 121 | RING-CH-typePROSITE-ProRule annotationAdd BLAST | 62 |
GO - Molecular functioni
- ubiquitin-protein transferase activity Source: TAIR
- zinc ion binding Source: InterPro
GO - Biological processi
- cuticle development Source: CACAO
- cutin biosynthetic process Source: TAIR
- isoprenoid biosynthetic process Source: TAIR
- positive regulation of isopentenyl diphosphate biosynthetic process, mevalonate pathway Source: TAIR
- response to water deprivation Source: TAIR
- suberin biosynthetic process Source: TAIR
- ubiquitin-dependent ERAD pathway Source: GO_Central
- wax biosynthetic process Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Protein family/group databases
TCDBi | 3.A.16.1.5, the endoplasmic reticular retrotranslocon (er-rt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Probable E3 ubiquitin ligase SUD1 (EC:2.3.2.27)Alternative name(s): Protein ECERIFERUM 9 Protein SUPPRESSOR OF DRY2 DEFFECTS 1 Short name: AtSUD1 RING-type E3 ubiquitin transferase SUD1Curated RING/U-box domain-containing protein |
Gene namesi | Name:SUD1 Synonyms:CER9 Ordered Locus Names:At4g34100 ORF Names:F28A23.140 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G34100 |
TAIRi | locus:2124251, AT4G34100 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- integral component of endoplasmic reticulum membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 157 – 177 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 339 – 359 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 362 – 382 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 462 – 482 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 489 – 509 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 525 – 545 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 572 – 592 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 630 – 650 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 669 – 689 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 796 – 816 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 844 – 864 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 894 – 914 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 923 – 943 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 982 – 1002 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1017 – 1036 | HelicalSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Semiglossy stem. Elevated drought tolerance and reduced transpiration rate. Elevated amounts of 18-carbon-length cutin monomers and shift in the cuticular wax profile toward the very-long-chain free fatty acids tetracosanoic acid (C24) and hexacosanoic acid (C26).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 114 | C → Y in cer9-1; Semiglossy shoots. 1 Publication | 1 | |
Mutagenesisi | 218 | G → R in sud1-1; No visible phenotype except glossy shoots, but is able to suppress the dry2 phenotype. 1 Publication | 1 | |
Mutagenesisi | 244 | R → K in sud1-3; Able to suppress the dry2 phenotype. 1 Publication | 1 | |
Mutagenesisi | 360 | G → E in sud1-2; Able to suppress the dry2 phenotype. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422766 | 1 – 1108 | Probable E3 ubiquitin ligase SUD1Add BLAST | 1108 |
Proteomic databases
PaxDbi | F4JKK0 |
PRIDEi | F4JKK0 |
ProteomicsDBi | 228398 [F4JKK0-1] |
PTM databases
SwissPalmi | F4JKK0 |
Expressioni
Tissue specificityi
Expressed in cotyledons, leaves, roots, stems, inflorescences and siliques. Expression higher at the top than at the base of the stem.1 Publication
Developmental stagei
Constitutively expressed throughout development.1 Publication
Gene expression databases
ExpressionAtlasi | F4JKK0, baseline and differential |
Genevisiblei | F4JKK0, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 14838, 1 interactor |
IntActi | F4JKK0, 1 interactor |
STRINGi | 3702.AT4G34100.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 60 | DisorderedSequence analysisAdd BLAST | 60 | |
Regioni | 237 – 273 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 762 – 784 | DisorderedSequence analysisAdd BLAST | 23 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 286 – 308 | Sequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 10 – 51 | Polar residuesSequence analysisAdd BLAST | 42 |
Domaini
The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 60 – 121 | RING-CH-typePROSITE-ProRule annotationAdd BLAST | 62 |
Keywords - Domaini
Coiled coil, Transit peptide, Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1609, Eukaryota |
InParanoidi | F4JKK0 |
OMAi | WLPIRIL |
OrthoDBi | 170933at2759 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR011016, Znf_RING-CH IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF12906, RINGv, 1 hit |
SMARTi | View protein in SMART SM00744, RINGv, 1 hit |
PROSITEi | View protein in PROSITE PS51292, ZF_RING_CH, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: F4JKK0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEISPADSLS ISGAAASEVV SEPSVSSSSS SSSPNQASPN PFSNMDPAVS
60 70 80 90 100
TATGSRYVDD DEDEEDVCRI CRNPGDADNP LRYPCACSGS IKFVHQDCLL
110 120 130 140 150
QWLNHSNARQ CEVCKHPFSF SPVYADNAPS RLPFQEFVVG IAMKACHVLQ
160 170 180 190 200
FFLRLSFVLS VWLLTIPFIT FWIWRLAFVR TFGEAQRLFL SHISTTVILT
210 220 230 240 250
DCLHGFLLSA SIVFIFLGAT SLRDYFRHLR ELGGQEERDD DVDRNGARAA
260 270 280 290 300
RRPAGQANRN LAGEGNGEDA GDQGAAVGQI ARRNPENVLA RLDIQAARLE
310 320 330 340 350
AQVEQMFDGL DDADGAEDVP FDELVGMQGP VFHLVENAFT VLASNMIFLG
360 370 380 390 400
VVIFVPFTLG RIILYHVSWL FAAARGPAVA ASLHLTDTGL SLENITLKSA
410 420 430 440 450
LTAVSNLTSE GQGNGLLGQL TEMMKVNGSE LNGANNTLSV ATDLLKGSTV
460 470 480 490 500
GASKLSDITT LAVGYMFIVF LVFLYLGIIA LIRYAKGEPL TVGRFYGIAS
510 520 530 540 550
IVEAVPSLLR QFLAAMRHLM TMIKVAFLLV IELGVFPLMC GWWLDVCTVR
560 570 580 590 600
MFGKTMSHRV QFLSISPLAS SLVHWVVGIM YMLQISIFVS LLRGVLRPGV
610 620 630 640 650
LYFLRDPADP NYNPFRDLID DPVHKHARRV LLSVAVYGSL IVMLVFLPVK
660 670 680 690 700
LAIRMAPSIF PLDISVSDPF TEIPADMLLF QICIPFIIEH FRLRTTIKSL
710 720 730 740 750
LRCWFTGVGW ALGLTDFLLP RPEDNIGQDN GNGEPGRQNR AQVLQVGGPD
760 770 780 790 800
RAMAALPVAD DPNRSRLRAG NVNTGEEYED DDEQSDSDRY NFVVRIILLL
810 820 830 840 850
LVAWVTLLLF NSALIVVPVS LGRALFSAIP ILPITHGIKC NDLYAFVIGT
860 870 880 890 900
YAFWTTISGA RYAIEHVKSK RTSVLLNQIW KWCGIVFKSS VLLAIWVFII
910 920 930 940 950
PVLIGLLFEL LVIVPMRVPV DESPVFLLYQ DWALGLIFLK IWTRLVMLDH
960 970 980 990 1000
MLPIVDDSWR AKFERVREDG FSRLQGLWVL REIVFPIVMK LLTALCVPYV
1010 1020 1030 1040 1050
LARGVFPMLG YPLVVNSAVY RFAWIGCLSV SLFCFCAKRC HVWFRNLHNS
1060 1070 1080 1090 1100
IRDDRYLIGR RLHNFGEAAL ANQNQNQSSE DAGDGVLIGR EGDVDNGLRL
RRAIQQEA
Sequence cautioni
The sequence CAA17562 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB80127 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 816 | V → D in BAF01965 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046615 | 788 – 789 | DR → E in isoform 2. Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL021961 Genomic DNA Translation: CAA17562.1 Sequence problems. AL161584 Genomic DNA Translation: CAB80127.1 Sequence problems. CP002687 Genomic DNA Translation: AEE86323.1 CP002687 Genomic DNA Translation: AEE86324.1 AK230154 mRNA Translation: BAF01965.1 |
PIRi | T05426 |
RefSeqi | NP_001119113.1, NM_001125641.1 [F4JKK0-2] NP_195136.3, NM_119571.5 [F4JKK0-1] |
Genome annotation databases
EnsemblPlantsi | AT4G34100.1; AT4G34100.1; AT4G34100 [F4JKK0-1] AT4G34100.2; AT4G34100.2; AT4G34100 [F4JKK0-2] |
GeneIDi | 829556 |
Gramenei | AT4G34100.1; AT4G34100.1; AT4G34100 [F4JKK0-1] AT4G34100.2; AT4G34100.2; AT4G34100 [F4JKK0-2] |
KEGGi | ath:AT4G34100 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL021961 Genomic DNA Translation: CAA17562.1 Sequence problems. AL161584 Genomic DNA Translation: CAB80127.1 Sequence problems. CP002687 Genomic DNA Translation: AEE86323.1 CP002687 Genomic DNA Translation: AEE86324.1 AK230154 mRNA Translation: BAF01965.1 |
PIRi | T05426 |
RefSeqi | NP_001119113.1, NM_001125641.1 [F4JKK0-2] NP_195136.3, NM_119571.5 [F4JKK0-1] |
3D structure databases
SMRi | F4JKK0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 14838, 1 interactor |
IntActi | F4JKK0, 1 interactor |
STRINGi | 3702.AT4G34100.1 |
Protein family/group databases
TCDBi | 3.A.16.1.5, the endoplasmic reticular retrotranslocon (er-rt) family |
PTM databases
SwissPalmi | F4JKK0 |
Proteomic databases
PaxDbi | F4JKK0 |
PRIDEi | F4JKK0 |
ProteomicsDBi | 228398 [F4JKK0-1] |
Genome annotation databases
EnsemblPlantsi | AT4G34100.1; AT4G34100.1; AT4G34100 [F4JKK0-1] AT4G34100.2; AT4G34100.2; AT4G34100 [F4JKK0-2] |
GeneIDi | 829556 |
Gramenei | AT4G34100.1; AT4G34100.1; AT4G34100 [F4JKK0-1] AT4G34100.2; AT4G34100.2; AT4G34100 [F4JKK0-2] |
KEGGi | ath:AT4G34100 |
Organism-specific databases
Araporti | AT4G34100 |
TAIRi | locus:2124251, AT4G34100 |
Phylogenomic databases
eggNOGi | KOG1609, Eukaryota |
InParanoidi | F4JKK0 |
OMAi | WLPIRIL |
OrthoDBi | 170933at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:F4JKK0 |
Gene expression databases
ExpressionAtlasi | F4JKK0, baseline and differential |
Genevisiblei | F4JKK0, AT |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR011016, Znf_RING-CH IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF12906, RINGv, 1 hit |
SMARTi | View protein in SMART SM00744, RINGv, 1 hit |
PROSITEi | View protein in PROSITE PS51292, ZF_RING_CH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SUD1_ARATH | |
Accessioni | F4JKK0Primary (citable) accession number: F4JKK0 Secondary accession number(s): F4JKK1, O49494, Q0WLP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2013 |
Last sequence update: | June 28, 2011 | |
Last modified: | September 29, 2021 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways