Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thiamine pyrophosphokinase 2

Gene

TPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase 1 (TPK1), Thiamine pyrophosphokinase 2 (TPK2), Thiamine pyrophosphokinase (TPK1), Thiamine pyrophosphokinase (AXX17_At2g42340), Thiamine pyrophosphokinase (AXX17_At2g42340), Thiamine pyrophosphokinase (AXX17_At1g02080)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • thiamine binding Source: InterPro
  • thiamine diphosphokinase activity Source: TAIR

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: GO_Central
  • thiamine metabolic process Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT2G44750-MONOMER
BRENDAi2.7.6.2 399
ReactomeiR-ATH-196819 Vitamin B1 (thiamin) metabolism
UniPathwayi
UPA00060;UER00597

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase 2 (EC:2.7.6.2)
Short name:
AtTPK2
Alternative name(s):
Thiamine kinase 2
Gene namesi
Name:TPK2
Ordered Locus Names:At2g44750
ORF Names:F16B22.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G44750
TAIRilocus:2042436 AT2G44750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Tpk1 and tpk2 double mutants exhibit a seedling lethal phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239671 – 267Thiamine pyrophosphokinase 2Add BLAST267

Proteomic databases

PaxDbiF4IV16
PRIDEiF4IV16

Expressioni

Tissue specificityi

Expressed in leaves and at lower levels in flowers.1 Publication

Gene expression databases

ExpressionAtlasiF4IV16 baseline and differential
GenevisibleiF4IV16 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44750.2

Structurei

3D structure databases

ProteinModelPortaliF4IV16
SMRiF4IV16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153 Eukaryota
COG1564 LUCA
HOGENOMiHOG000180834
KOiK00949
OMAiTGNLKYC
OrthoDBiEOG09360JCF
PhylomeDBiF4IV16

Family and domain databases

CDDicd07995 TPK, 1 hit
Gene3Di3.40.50.10240, 1 hit
InterProiView protein in InterPro
IPR006282 Thi_PPkinase
IPR016966 Thiamin_pyrophosphokinase_euk
IPR007373 Thiamin_PyroPKinase_B1-bd
IPR036371 TPK_B1-bd_sf
IPR007371 TPK_catalytic
IPR036759 TPK_catalytic_sf
PfamiView protein in Pfam
PF04265 TPK_B1_binding, 1 hit
PF04263 TPK_catalytic, 1 hit
PIRSFiPIRSF031057 Thiamin_pyrophosphokinase, 1 hit
SMARTiView protein in SMART
SM00983 TPK_B1_binding, 1 hit
SUPFAMiSSF63862 SSF63862, 1 hit
SSF63999 SSF63999, 1 hit
TIGRFAMsiTIGR01378 thi_PPkinase, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: F4IV16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLSAMDVMIH SSSFLLPCDE TCGTRYALVV LNQNLPRFTP LLWEHAKLRL
60 70 80 90 100
CADGGANRIY DELPLFFPHE DPFVIRNRYK PDVIKGDMDS IRRDVLDFYV
110 120 130 140 150
YWGTKVIDES HDQDTTDLDK CISYIRHSTL NQESSRLQIL ATGALGGRFD
160 170 180 190 200
HEAGNLNVLY RYPDTRIVLL SDDCLIQLLP KTHRHEIHIH SSLQGPHCGL
210 220 230 240 250
IPIGTPSANT TTSGLKWDLS NTEMRFGGLI STSNLVKEEI ITVESDSDLL
260
WTISIKKTGL PVQDHKP
Length:267
Mass (Da):30,214
Last modified:June 28, 2011 - v1
Checksum:i4EB8B19CE2F12699
GO
Isoform 2 (identifier: F4IV16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-138: Missing.

Show »
Length:265
Mass (Da):29,973
Checksum:iDC19226CBFAF12BC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9FL25KCO2_ARATH
Two-pore potassium channel 2
TPK2 KCO2, At5g46370, MPL12.17
443Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053287137 – 138Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA Translation: AAC27477.2
CP002685 Genomic DNA Translation: AEC10463.1
CP002685 Genomic DNA Translation: AEC10464.1
AY042898 mRNA Translation: AAK68838.1
AY081493 mRNA Translation: AAM10055.1
PIRiT01602
RefSeqiNP_566026.1, NM_130040.3 [F4IV16-2]
NP_850424.1, NM_180093.2 [F4IV16-1]
UniGeneiAt.48590
At.70253

Genome annotation databases

EnsemblPlantsiAT2G44750.1; AT2G44750.1; AT2G44750 [F4IV16-2]
AT2G44750.2; AT2G44750.2; AT2G44750 [F4IV16-1]
GeneIDi819084
GrameneiAT2G44750.1; AT2G44750.1; AT2G44750 [F4IV16-2]
AT2G44750.2; AT2G44750.2; AT2G44750 [F4IV16-1]
KEGGiath:AT2G44750

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTPK2_ARATH
AccessioniPrimary (citable) accession number: F4IV16
Secondary accession number(s): O80514, Q94B25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again