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UniProtKB - F4I4K7 (FRO3_ARATH)
Protein
Ferric reduction oxidase 3, mitochondrial
Gene
FRO3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Ferric chelate reductase involved in iron reduction in roots. May participate in the transport of electrons to a Fe3+ ion via FAD and heme intermediates.
1 PublicationMiscellaneous
It is not clear whether or not FRO3 functions in iron import to mitochondria or is involved in iron efflux to the cytosol.
Catalytic activityi
- EC:1.16.1.7
Cofactori
FADCurated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 233 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 247 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 307 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 320 | Iron (heme axial ligand)Curated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 395 – 398 | FADSequence analysis | 4 | |
Nucleotide bindingi | 443 – 446 | NADSequence analysis | 4 |
GO - Molecular functioni
- ferric-chelate reductase (NADH) activity Source: UniProtKB-EC
- ferric-chelate reductase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- ion transport Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Ion transport, Transport |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, NAD |
Enzyme and pathway databases
BRENDAi | 1.16.1.10, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Ferric reduction oxidase 3, mitochondrial (EC:1.16.1.7)Short name: AtFRO3 Alternative name(s): Ferric-chelate reductase 3 |
Gene namesi | Name:FRO3 Synonyms:FROHC Ordered Locus Names:At1g23020 ORF Names:F19G10.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G23020 |
TAIRi | locus:2017789, AT1G23020 |
Subcellular locationi
Mitochondrion
- Mitochondrion membrane Curated; Multi-pass membrane protein Curated
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 40 – 59 | HelicalBy similarityAdd BLAST | 20 | |
Transmembranei | 86 – 104 | HelicalBy similarityAdd BLAST | 19 | |
Transmembranei | 140 – 163 | HelicalBy similarityAdd BLAST | 24 | |
Transmembranei | 232 – 255 | HelicalBy similarityAdd BLAST | 24 | |
Transmembranei | 306 – 330 | HelicalBy similarityAdd BLAST | 25 | |
Transmembranei | 353 – 373 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 564 – 586 | HelicalBy similarityAdd BLAST | 23 | |
Transmembranei | 606 – 627 | HelicalBy similarityAdd BLAST | 22 |
Keywords - Cellular componenti
Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 23 | MitochondrionSequence analysisAdd BLAST | 23 | |
ChainiPRO_0000413201 | 24 – 717 | Ferric reduction oxidase 3, mitochondrialAdd BLAST | 694 |
Proteomic databases
PaxDbi | F4I4K7 |
PRIDEi | F4I4K7 |
ProteomicsDBi | 248553 [F4I4K7-1] |
Expressioni
Tissue specificityi
Expressed in root steele. Detected in shoots, leaves, stems, siliques, flowers and cotyledons.3 Publications
Inductioni
Up-regulated in roots and shoots by iron deficiency and copper deficiency.3 Publications
Gene expression databases
ExpressionAtlasi | F4I4K7, baseline and differential |
Genevisiblei | F4I4K7, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 317 | Ferric oxidoreductaseAdd BLAST | 120 | |
Domaini | 346 – 451 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 106 |
Sequence similaritiesi
Belongs to the ferric reductase (FRE) family.Curated
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
InParanoidi | F4I4K7 |
OMAi | HQVANIC |
OrthoDBi | 190356at2759 |
Family and domain databases
Gene3Di | 3.40.50.80, 2 hits |
InterProi | View protein in InterPro IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: F4I4K7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAARGRLVVA RGNRSFSSII RKYSLKRETN KKVIKNVIKL LTMVILMGTV
60 70 80 90 100
VIWIMMPTST YKKIWLKSMR AKLGKSIYFG KPGVNLLVYM FPMILLASLG
110 120 130 140 150
SIYLHLKKQT RVNQFNSRMD RKKIDKFGAL KRPMLVKAGL GIVTVTEVMF
160 170 180 190 200
LMMFMALLLW SLANYFYHTF VTITPQSLPT DGDNLWQARL DSIAVRLGLT
210 220 230 240 250
GNICLGFLFY PVARGSSLLA AVGLTSESST KYHIWLGNLV MTLFTSHGLC
260 270 280 290 300
YCIYWISTNQ VSQMLEWDRT GISHLAGEIA LVAGLLMWAT TFPAIRRRFF
310 320 330 340 350
EVFFYTHYLY MVFMLFFVFH VGISYALISF PGFYIFMVDR FLRFLQSRNN
360 370 380 390 400
VKLVSARVLP CETVELNFSK NPMLMYSPTS ILFVNIPSIS KLQWHPFTIT
410 420 430 440 450
SSSKLEPKKL SVMIKSQGKW SSKLHHMLAS SNQIDHLAVS VEGPYGPAST
460 470 480 490 500
DYLRHDSLVM VSGGSGITPF ISIIRDLLYV SSTNAYKTPK ITLICAFKNS
510 520 530 540 550
SDLSMLNLIL PNSTEISSFI DIQIKAFVTR EKVSTCNMNI IKTLSFKPYV
560 570 580 590 600
SDQPISPILG PNSWLWLATI LSSSFMIFII IIAIISRYHI YPIDQSSKEY
610 620 630 640 650
TSAYTSLIYL LAISISVVAT STVAMLCNKK SYFKGLYQNV DALSPLMIES
660 670 680 690 700
SPDQLLPEFT NIHYGERPNL NKLLVGLKGS SVGVLVCGPR KMREEVAKIC
710
SFGSAANLQF ESISFNW
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8ATB2 | A0A1P8ATB2_ARATH | Ferric reduction oxidase 3 | FRO3 ATFRO3, FERRIC REDUCTION OXIDASE 3, ferric reduction oxidase 3, At1g23020, F19G10.4 | 690 | Annotation score: | ||
A0A1P8ATD5 | A0A1P8ATD5_ARATH | Ferric reduction oxidase 3 | FRO3 ATFRO3, FERRIC REDUCTION OXIDASE 3, ferric reduction oxidase 3, At1g23020, F19G10.4 | 694 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041872 | 1 – 27 | MAARG…YSLKR → MVLG in isoform 3. CuratedAdd BLAST | 27 | |
Alternative sequenceiVSP_041873 | 117 | Missing in isoform 2 and isoform 3. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF000657 Genomic DNA Translation: AAB72168.1 CP002684 Genomic DNA Translation: AEE30322.1 CP002684 Genomic DNA Translation: AEE30323.1 CP002684 Genomic DNA Translation: ANM59873.1 CP002684 Genomic DNA Translation: ANM59874.1 CP002684 Genomic DNA Translation: ANM59875.1 |
PIRi | C86364 |
RefSeqi | NP_001185067.1, NM_001198138.1 [F4I4K7-1] NP_001319065.1, NM_001332575.1 [F4I4K7-2] NP_001322197.1, NM_001332576.1 [F4I4K7-3] NP_001322198.1, NM_001332577.1 [F4I4K7-3] NP_173715.2, NM_102150.4 [F4I4K7-2] |
Genome annotation databases
EnsemblPlantsi | AT1G23020.1; AT1G23020.1; AT1G23020 [F4I4K7-2] AT1G23020.2; AT1G23020.2; AT1G23020 [F4I4K7-1] AT1G23020.3; AT1G23020.3; AT1G23020 [F4I4K7-3] AT1G23020.4; AT1G23020.4; AT1G23020 [F4I4K7-3] AT1G23020.5; AT1G23020.5; AT1G23020 [F4I4K7-2] |
GeneIDi | 838910 |
Gramenei | AT1G23020.1; AT1G23020.1; AT1G23020 [F4I4K7-2] AT1G23020.2; AT1G23020.2; AT1G23020 [F4I4K7-1] AT1G23020.3; AT1G23020.3; AT1G23020 [F4I4K7-3] AT1G23020.4; AT1G23020.4; AT1G23020 [F4I4K7-3] AT1G23020.5; AT1G23020.5; AT1G23020 [F4I4K7-2] |
KEGGi | ath:AT1G23020 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF000657 Genomic DNA Translation: AAB72168.1 CP002684 Genomic DNA Translation: AEE30322.1 CP002684 Genomic DNA Translation: AEE30323.1 CP002684 Genomic DNA Translation: ANM59873.1 CP002684 Genomic DNA Translation: ANM59874.1 CP002684 Genomic DNA Translation: ANM59875.1 |
PIRi | C86364 |
RefSeqi | NP_001185067.1, NM_001198138.1 [F4I4K7-1] NP_001319065.1, NM_001332575.1 [F4I4K7-2] NP_001322197.1, NM_001332576.1 [F4I4K7-3] NP_001322198.1, NM_001332577.1 [F4I4K7-3] NP_173715.2, NM_102150.4 [F4I4K7-2] |
3D structure databases
SMRi | F4I4K7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G23020.2 |
Proteomic databases
PaxDbi | F4I4K7 |
PRIDEi | F4I4K7 |
ProteomicsDBi | 248553 [F4I4K7-1] |
Genome annotation databases
EnsemblPlantsi | AT1G23020.1; AT1G23020.1; AT1G23020 [F4I4K7-2] AT1G23020.2; AT1G23020.2; AT1G23020 [F4I4K7-1] AT1G23020.3; AT1G23020.3; AT1G23020 [F4I4K7-3] AT1G23020.4; AT1G23020.4; AT1G23020 [F4I4K7-3] AT1G23020.5; AT1G23020.5; AT1G23020 [F4I4K7-2] |
GeneIDi | 838910 |
Gramenei | AT1G23020.1; AT1G23020.1; AT1G23020 [F4I4K7-2] AT1G23020.2; AT1G23020.2; AT1G23020 [F4I4K7-1] AT1G23020.3; AT1G23020.3; AT1G23020 [F4I4K7-3] AT1G23020.4; AT1G23020.4; AT1G23020 [F4I4K7-3] AT1G23020.5; AT1G23020.5; AT1G23020 [F4I4K7-2] |
KEGGi | ath:AT1G23020 |
Organism-specific databases
Araporti | AT1G23020 |
TAIRi | locus:2017789, AT1G23020 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
InParanoidi | F4I4K7 |
OMAi | HQVANIC |
OrthoDBi | 190356at2759 |
Enzyme and pathway databases
BRENDAi | 1.16.1.10, 399 |
Miscellaneous databases
PROi | PR:F4I4K7 |
Gene expression databases
ExpressionAtlasi | F4I4K7, baseline and differential |
Genevisiblei | F4I4K7, AT |
Family and domain databases
Gene3Di | 3.40.50.80, 2 hits |
InterProi | View protein in InterPro IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FRO3_ARATH | |
Accessioni | F4I4K7Primary (citable) accession number: F4I4K7 Secondary accession number(s): F4I4K6, O23122 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | June 28, 2011 | |
Last modified: | February 23, 2022 | |
This is version 72 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families