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Protein

Ferric reduction oxidase 3, mitochondrial

Gene

FRO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ferric chelate reductase involved in iron reduction in roots. May participate in the transport of electrons to a Fe3+ ion via FAD and heme intermediates.1 Publication

Miscellaneous

It is not clear whether or not FRO3 functions in iron import to mitochondria or is involved in iron efflux to the cytosol.

Catalytic activityi

2 Fe(II)-siderophore + NAD+ + H+ = 2 Fe(III)-siderophore + NADH.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi233Iron (heme axial ligand)Curated1
Metal bindingi247Iron (heme axial ligand)Curated1
Metal bindingi307Iron (heme axial ligand)Curated1
Metal bindingi320Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi395 – 398FADSequence analysis4
Nucleotide bindingi443 – 446NADSequence analysis4

GO - Molecular functioni

  • ferric-chelate reductase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Ion transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NAD

Enzyme and pathway databases

ReactomeiR-ATH-3299685 Detoxification of Reactive Oxygen Species

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reduction oxidase 3, mitochondrial (EC:1.16.1.7)
Short name:
AtFRO3
Alternative name(s):
Ferric-chelate reductase 3
Gene namesi
Name:FRO3
Synonyms:FROHC
Ordered Locus Names:At1g23020
ORF Names:F19G10.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G23020
TAIRilocus:2017789 AT1G23020

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei40 – 59HelicalBy similarityAdd BLAST20
Transmembranei86 – 104HelicalBy similarityAdd BLAST19
Transmembranei140 – 163HelicalBy similarityAdd BLAST24
Transmembranei232 – 255HelicalBy similarityAdd BLAST24
Transmembranei306 – 330HelicalBy similarityAdd BLAST25
Transmembranei353 – 373HelicalBy similarityAdd BLAST21
Transmembranei564 – 586HelicalBy similarityAdd BLAST23
Transmembranei606 – 627HelicalBy similarityAdd BLAST22

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionSequence analysisAdd BLAST23
ChainiPRO_000041320124 – 717Ferric reduction oxidase 3, mitochondrialAdd BLAST694

Proteomic databases

PaxDbiF4I4K7
PRIDEiF4I4K7

Expressioni

Tissue specificityi

Expressed in root steele. Detected in shoots, leaves, stems, siliques, flowers and cotyledons.3 Publications

Inductioni

Up-regulated in roots and shoots by iron deficiency and copper deficiency.3 Publications

Gene expression databases

ExpressionAtlasiF4I4K7 baseline and differential
GenevisibleiF4I4K7 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G23020.2

Structurei

3D structure databases

ProteinModelPortaliF4I4K7
SMRiF4I4K7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini198 – 317Ferric oxidoreductaseAdd BLAST120
Domaini346 – 451FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi299 – 344Phe-richAdd BLAST46
Compositional biasi577 – 593Ile-richAdd BLAST17

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
HOGENOMiHOG000006154
InParanoidiF4I4K7
KOiK00521
OMAiFKPYVSD
OrthoDBiEOG093603NP

Family and domain databases

Gene3Di3.40.50.80, 2 hits
InterProiView protein in InterPro
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR039261 FNR_nucleotide_bd
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
SUPFAMiSSF52343 SSF52343, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: F4I4K7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARGRLVVA RGNRSFSSII RKYSLKRETN KKVIKNVIKL LTMVILMGTV
60 70 80 90 100
VIWIMMPTST YKKIWLKSMR AKLGKSIYFG KPGVNLLVYM FPMILLASLG
110 120 130 140 150
SIYLHLKKQT RVNQFNSRMD RKKIDKFGAL KRPMLVKAGL GIVTVTEVMF
160 170 180 190 200
LMMFMALLLW SLANYFYHTF VTITPQSLPT DGDNLWQARL DSIAVRLGLT
210 220 230 240 250
GNICLGFLFY PVARGSSLLA AVGLTSESST KYHIWLGNLV MTLFTSHGLC
260 270 280 290 300
YCIYWISTNQ VSQMLEWDRT GISHLAGEIA LVAGLLMWAT TFPAIRRRFF
310 320 330 340 350
EVFFYTHYLY MVFMLFFVFH VGISYALISF PGFYIFMVDR FLRFLQSRNN
360 370 380 390 400
VKLVSARVLP CETVELNFSK NPMLMYSPTS ILFVNIPSIS KLQWHPFTIT
410 420 430 440 450
SSSKLEPKKL SVMIKSQGKW SSKLHHMLAS SNQIDHLAVS VEGPYGPAST
460 470 480 490 500
DYLRHDSLVM VSGGSGITPF ISIIRDLLYV SSTNAYKTPK ITLICAFKNS
510 520 530 540 550
SDLSMLNLIL PNSTEISSFI DIQIKAFVTR EKVSTCNMNI IKTLSFKPYV
560 570 580 590 600
SDQPISPILG PNSWLWLATI LSSSFMIFII IIAIISRYHI YPIDQSSKEY
610 620 630 640 650
TSAYTSLIYL LAISISVVAT STVAMLCNKK SYFKGLYQNV DALSPLMIES
660 670 680 690 700
SPDQLLPEFT NIHYGERPNL NKLLVGLKGS SVGVLVCGPR KMREEVAKIC
710
SFGSAANLQF ESISFNW
Note: Derived from EST data. No experimental confirmation available.
Length:717
Mass (Da):80,934
Last modified:June 28, 2011 - v1
Checksum:i9213B93C8E99F6A1
GO
Isoform 2 (identifier: F4I4K7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-117: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:716
Mass (Da):80,847
Checksum:i9ACF3680CDB53DC2
GO
Isoform 3 (identifier: F4I4K7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: MAARGRLVVARGNRSFSSIIRKYSLKR → MVLG
     117-117: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:693
Mass (Da):78,172
Checksum:i05D2FBC7AEA4AF32
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0418721 – 27MAARG…YSLKR → MVLG in isoform 3. CuratedAdd BLAST27
Alternative sequenceiVSP_041873117Missing in isoform 2 and isoform 3. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000657 Genomic DNA Translation: AAB72168.1
CP002684 Genomic DNA Translation: AEE30322.1
CP002684 Genomic DNA Translation: AEE30323.1
CP002684 Genomic DNA Translation: ANM59873.1
CP002684 Genomic DNA Translation: ANM59874.1
CP002684 Genomic DNA Translation: ANM59875.1
PIRiC86364
RefSeqiNP_001185067.1, NM_001198138.1 [F4I4K7-1]
NP_001319065.1, NM_001332575.1 [F4I4K7-2]
NP_001322197.1, NM_001332576.1 [F4I4K7-3]
NP_001322198.1, NM_001332577.1 [F4I4K7-3]
NP_173715.2, NM_102150.4 [F4I4K7-2]
UniGeneiAt.41573

Genome annotation databases

EnsemblPlantsiAT1G23020.1; AT1G23020.1; AT1G23020 [F4I4K7-2]
AT1G23020.2; AT1G23020.2; AT1G23020 [F4I4K7-1]
AT1G23020.3; AT1G23020.3; AT1G23020 [F4I4K7-3]
AT1G23020.4; AT1G23020.4; AT1G23020 [F4I4K7-3]
AT1G23020.5; AT1G23020.5; AT1G23020 [F4I4K7-2]
GeneIDi838910
GrameneiAT1G23020.1; AT1G23020.1; AT1G23020 [F4I4K7-2]
AT1G23020.2; AT1G23020.2; AT1G23020 [F4I4K7-1]
AT1G23020.3; AT1G23020.3; AT1G23020 [F4I4K7-3]
AT1G23020.4; AT1G23020.4; AT1G23020 [F4I4K7-3]
AT1G23020.5; AT1G23020.5; AT1G23020 [F4I4K7-2]
KEGGiath:AT1G23020

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFRO3_ARATH
AccessioniPrimary (citable) accession number: F4I4K7
Secondary accession number(s): F4I4K6, O23122
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: June 28, 2011
Last modified: July 18, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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