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Protein

Subtilisin-like protease SBT2.4

Gene

SBT2.4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease required for epidermal surface formation in embryos and juvenile plants (PubMed:11731449, PubMed:17376810, PubMed:23318634). Involved in embryonic cuticle formation downstream of BHLH95/ZOU (PubMed:23318634).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174Charge relay systemPROSITE-ProRule annotation1
Active sitei252Charge relay systemPROSITE-ProRule annotation1
Active sitei618Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cuticle development Source: TAIR

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.014

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-like protease SBT2.41 Publication (EC:3.4.21.-Curated)
Alternative name(s):
Protein ABNORMAL LEAF SHAPE 11 Publication
Subtilase subfamily 2 member 41 Publication
Short name:
AtSBT2.41 Publication
Gene namesi
Name:SBT2.41 Publication
Synonyms:ALE11 Publication
Ordered Locus Names:At1g62340Imported
ORF Names:F2401.7Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G62340
TAIRilocus:2027139 AT1G62340

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Seedlings lethality when homozygous due to water loss. Mutant seedling grown under high humidity can survive and show small, crinkled cotyledons and fused leaves due to impaired cuticule formation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi230G → E in ale1-2; Seedlings lethality when homozygous due to water loss. Mutant seedling grown under high humidity can survive and show small, crinkled cotyledons and fused leaves. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000043196628 – 832Subtilisin-like protease SBT2.4Sequence analysisAdd BLAST805

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi196N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi238N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi426N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi761N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi774N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi800N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiF4HYR6
PRIDEiF4HYR6

Expressioni

Developmental stagei

Expressed in the endosperm cells during embryo development.1 Publication

Gene expression databases

ExpressionAtlasiF4HYR6 differential
GenevisibleiF4HYR6 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G62340.1

Structurei

3D structure databases

ProteinModelPortaliF4HYR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 691Peptidase S8Sequence analysisAdd BLAST523
Domaini425 – 524PASequence analysisAdd BLAST100

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIUP Eukaryota
ENOG410YDJ9 LUCA
HOGENOMiHOG000238262
InParanoidiF4HYR6
OMAiGHQIWAA
OrthoDBiEOG093602PZ

Family and domain databases

CDDicd04852 Peptidases_S8_3, 1 hit
Gene3Di3.40.50.200, 2 hits
InterProiView protein in InterPro
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR034197 Peptidases_S8_3
IPR010259 S8pro/Inhibitor_I9
PfamiView protein in Pfam
PF05922 Inhibitor_I9, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF52743 SSF52743, 3 hits
PROSITEiView protein in PROSITE
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F4HYR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METNPRKLRS YSYICLIVCI FVLVVCAILS RAEEKEGKGE NDDHIPKIYS
60 70 80 90 100
ILVEGEPLAF RASTNINSKA MALEAKKIEE IHDEILGSTL EKGSYTKLYS
110 120 130 140 150
FKHVINAIAV RTTASQAKKL GKTKGVKAVE EDKGVKLMTT YTPDFLELPQ
160 170 180 190 200
QVWQKISNEG DRRAGEDIVI GFVDTGINPT HPSFAALDLT NPYSSNLSRL
210 220 230 240 250
HFSGDCEIGP FFPPGSCNGK IISARFFSAG ARASGALNSS LDILSPFDAS
260 270 280 290 300
GHGSHVASIA AGNAGVPVIV DGFFYGRASG MAPRSRIAVY KAIYPSIGTL
310 320 330 340 350
VDVIAAIDQA IMDGVDVLTL SVGPDEPPVD KPTVLGIFDL AMLLARKAGV
360 370 380 390 400
FVVQAVGNNG PSPSSVLSYS PWVVGVAAGN TDRSYPAPLI LDGGQTVQGV
410 420 430 440 450
GLSGPTLGAP LVQHRLVLAK DAVRTNGSVL QPLTRDIEEC QRPENFDPAA
460 470 480 490 500
VFGSIVICTF SDGFYNQMST VLAITQTART LGFMGFILIA NPRFGDYVAE
510 520 530 540 550
PVIFSAPGIL IPTVSAAQII LRYYEEKTFR DTRGVATQFG ARARIGEGRN
560 570 580 590 600
SVFAGKAPVV SRFSSRGPAF IDATRSPLDV LKPDILAPGH QIWGAWSLPS
610 620 630 640 650
AFDPILTGRS FAILSGTSMA TPHIAGIGAL IKQLNPSWTP AMIASAISTT
660 670 680 690 700
ANEYDSNGEI ISAEYYELSR LFPSNHFDHG AGHVNPARAL DPGLVLPAGF
710 720 730 740 750
EDYISFLCSL PNISPATIRD ATGVLCTTTL SHPANLNHPS VTISALKESL
760 770 780 790 800
VVRRSFQDVS NKTETYLGSV LPPNGTTVRL TPTWFTVPPQ KTQDLDIEFN
810 820 830
VTQVLNKFTF GEVVLTGSLN HIIRIPLSVK TI
Length:832
Mass (Da):89,066
Last modified:June 28, 2011 - v1
Checksum:i1BD16943C17913AC
GO

Sequence cautioni

The sequence AAF70850 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15C → W in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti36 – 42EGKGEND → QGKDENN in BAB70678 (PubMed:11731449).Curated7
Sequence conflicti51I → V in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti83D → E in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti108I → F in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti121G → R in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti154Q → P in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti197L → I in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti211F → L in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti238N → S in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti270V → I in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti285S → A in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti388P → S in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti546G → D in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti552 – 556VFAGK → IFAGQ in BAB70678 (PubMed:11731449).Curated5
Sequence conflicti574T → N in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti657N → T in BAB70678 (PubMed:11731449).Curated1
Sequence conflicti758D → N in BAB70678 (PubMed:11731449).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB060809 mRNA Translation: BAB70678.1
AC003113 Genomic DNA Translation: AAF70850.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33956.1
PIRiT01444
RefSeqiNP_564793.2, NM_104914.2
UniGeneiAt.26228

Genome annotation databases

EnsemblPlantsiAT1G62340.1; AT1G62340.1; AT1G62340
GeneIDi842532
GrameneiAT1G62340.1; AT1G62340.1; AT1G62340
KEGGiath:AT1G62340

Similar proteinsi

Entry informationi

Entry nameiSBT24_ARATH
AccessioniPrimary (citable) accession number: F4HYR6
Secondary accession number(s): O48798, Q948Q4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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