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UniProtKB - F4HUK6 (AAE1_ARATH)
Protein
Butanoate--CoA ligase AAE1
Gene
AAE1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the ligation of CoA on butanoate to produce butanoyl-CoA (PubMed:23300257).
Can also use hexanoate, pentanoate and 4-methylpentanoate as substrates with a lower efficiency (PubMed:23300257).
1 PublicationCatalytic activityi
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
GO - Molecular functioni
- ligase activity Source: UniProtKB-KW
GO - Biological processi
- fatty acid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Butanoate--CoA ligase AAE11 Publication (EC:6.2.1.-1 Publication)Alternative name(s): 4-methylpentanoate--CoA ligase AAE21 Publication (EC:6.2.1.-1 Publication) AMP-binding protein 11 Publication Short name: AtAMPBP11 Publication Acyl-activating enzyme 1, peroxisomal1 Publication Hexanoate--CoA ligase CCL31 Publication (EC:6.2.1.-1 Publication) Pentanoate--CoA ligase CCL31 Publication (EC:6.2.1.-1 Publication) |
Gene namesi | Name:AAE11 Publication Synonyms:AMPBP11 Publication Ordered Locus Names:At1g20560Imported ORF Names:F5M15.12Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G20560 |
TAIRi | locus:2030407, AT1G20560 |
Subcellular locationi
Peroxisome
- Peroxisome 1 Publication
Peroxisome
- peroxisome Source: TAIR
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000415713 | 1 – 556 | Butanoate--CoA ligase AAE1Add BLAST | 556 |
Proteomic databases
PaxDbi | F4HUK6 |
ProteomicsDBi | 245090 [F4HUK6-1] |
Expressioni
Tissue specificityi
Expressed in roots, leaves, stems, flowers and developing seeds.1 Publication
Gene expression databases
ExpressionAtlasi | F4HUK6, baseline and differential |
Genevisiblei | F4HUK6, AT |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 554 – 556 | Microbody targeting signalSequence analysis | 3 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1176, Eukaryota |
InParanoidi | F4HUK6 |
OMAi | SAMLDNC |
OrthoDBi | 312083at2759 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: F4HUK6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKMEGTIKSP ANYVPLTPIS FLDRSAVVYA DRVSIVYGSV KYTWRQTRDR
60 70 80 90 100
CVRIASALSQ LGISTGDVVS VLAPNVPAMV ELHFGVPMAG ALLCTLNIRH
110 120 130 140 150
DSSLVAVLLR HSGTKVIFAD HQFLQIAEGA CEILSNKGDK VPILVLIPEP
160 170 180 190 200
LTQSVSRKKR SEEMMEYEDV VAMGKSDFEV IRPTDECDAI SVNYTSGTTS
210 220 230 240 250
SPKGVVYSHR GAYLNSLAAV LLNEMHSSPT YLWTNPMFHC NGWCLLWGVT
260 270 280 290 300
AIGGTNICLR NVTAKAIFDN ISQHKVTHMG GAPTILNMII NAPESEQKPL
310 320 330 340 350
PGKVSFITGA APPPAHVIFK MEELGFSMFH SYGLTETYGP GTICTWKPEW
360 370 380 390 400
DSLPREEQAK MKARQGVNHL GLEEIQVKDP VTMRTLPADG VTMGEVVFRG
410 420 430 440 450
NTVMNGYLKN PEATKEAFKG GWFWSGDLGV KHPDGYIELK DRSKDIIISG
460 470 480 490 500
GENISSIEVE STLFTHPCVL EAAVVARPDE YWGETACAFV KLKDGSKASA
510 520 530 540 550
EELISYCRDR LPHYMAPRSI VFEDLPKTST GKVQKFVLRT KAKALVSLSK
KGRSKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA8MRP8 | A8MRP8_ARATH | Acyl activating enzyme 1 | AAE1 acyl activating enzyme 1, At1g20560, F5M15.12, F5M15_12 | 478 | Annotation score: |
Sequence cautioni
The sequence AAF79607 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF80642 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 128 | E → D in AAK50082 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 128 | E → D in AAL77740 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC027665 Genomic DNA Translation: AAF79607.1 Sequence problems. AC069251 Genomic DNA Translation: AAF80642.1 Sequence problems. CP002684 Genomic DNA Translation: AEE29986.1 AF372942 mRNA Translation: AAK50082.1 AY078039 mRNA Translation: AAL77740.1 AF503760 mRNA Translation: AAM28618.1 |
PIRi | G86338 |
RefSeqi | NP_564116.1, NM_101906.4 [F4HUK6-1] |
Genome annotation databases
EnsemblPlantsi | AT1G20560.1; AT1G20560.1; AT1G20560 [F4HUK6-1] |
GeneIDi | 838644 |
Gramenei | AT1G20560.1; AT1G20560.1; AT1G20560 [F4HUK6-1] |
KEGGi | ath:AT1G20560 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC027665 Genomic DNA Translation: AAF79607.1 Sequence problems. AC069251 Genomic DNA Translation: AAF80642.1 Sequence problems. CP002684 Genomic DNA Translation: AEE29986.1 AF372942 mRNA Translation: AAK50082.1 AY078039 mRNA Translation: AAL77740.1 AF503760 mRNA Translation: AAM28618.1 |
PIRi | G86338 |
RefSeqi | NP_564116.1, NM_101906.4 [F4HUK6-1] |
3D structure databases
AlphaFoldDBi | F4HUK6 |
SMRi | F4HUK6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G20560.1 |
Proteomic databases
PaxDbi | F4HUK6 |
ProteomicsDBi | 245090 [F4HUK6-1] |
Genome annotation databases
EnsemblPlantsi | AT1G20560.1; AT1G20560.1; AT1G20560 [F4HUK6-1] |
GeneIDi | 838644 |
Gramenei | AT1G20560.1; AT1G20560.1; AT1G20560 [F4HUK6-1] |
KEGGi | ath:AT1G20560 |
Organism-specific databases
Araporti | AT1G20560 |
TAIRi | locus:2030407, AT1G20560 |
Phylogenomic databases
eggNOGi | KOG1176, Eukaryota |
InParanoidi | F4HUK6 |
OMAi | SAMLDNC |
OrthoDBi | 312083at2759 |
Miscellaneous databases
PROi | PR:F4HUK6 |
Gene expression databases
ExpressionAtlasi | F4HUK6, baseline and differential |
Genevisiblei | F4HUK6, AT |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AAE1_ARATH | |
Accessioni | F4HUK6Primary (citable) accession number: F4HUK6 Secondary accession number(s): Q8LRT6 Q9LMW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 22, 2012 |
Last sequence update: | June 28, 2011 | |
Last modified: | May 25, 2022 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families