Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multifunctional fusion protein

Gene

murE

Organism
Selenomonas sputigena (strain ATCC 35185 / DSM 20758 / VPI D19B-28)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation
Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei153UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei181UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei187UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei189UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei386Meso-diaminopimelateUniRule annotation1
Binding sitei464Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei468Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi112 – 118ATPUniRule annotation7
Nucleotide bindingi611 – 617ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
Biological processCell cycle, Cell divisionUniRule annotationSAAS annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional fusion proteinUniRule annotation
Including the following 2 domains:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligaseUniRule annotation (EC:6.3.2.10UniRule annotation)
Alternative name(s):
D-alanyl-D-alanine-adding enzymeUniRule annotation
Gene namesi
Name:murEUniRule annotation
Synonyms:murFUniRule annotation
ORF Names:Selsp_1823Imported
OrganismiSelenomonas sputigena (strain ATCC 35185 / DSM 20758 / VPI D19B-28)Imported
Taxonomic identifieri546271 [NCBI]
Taxonomic lineageiBacteriaFirmicutesNegativicutesSelenomonadalesSelenomonadaceaeSelenomonas
Proteomesi
  • UP000011124 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei221N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 94Mur_ligaseInterPro annotationAdd BLAST69
Domaini110 – 315Mur_ligase_MInterPro annotationAdd BLAST206
Domaini335 – 419Mur_ligase_CInterPro annotationAdd BLAST85
Domaini526 – 598Mur_ligaseInterPro annotationAdd BLAST73
Domaini609 – 802Mur_ligase_MInterPro annotationAdd BLAST194
Domaini826 – 902Mur_ligase_CInterPro annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 155UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni410 – 413Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi410 – 413Meso-diaminopimelate recognition motifUniRule annotation4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotationSAAS annotation
Belongs to the MurCDEF family. MurF subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EEN Bacteria
COG0769 LUCA
COG0770 LUCA
KOiK15792

Family and domain databases

Gene3Di3.40.1190.10, 2 hits
3.90.190.20, 2 hits
HAMAPiMF_00208 MurE, 1 hit
MF_02019 MurF, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR000713 Mur_ligase_N
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
IPR005863 UDP-N-AcMur_synth
PfamiView protein in Pfam
PF01225 Mur_ligase, 2 hits
PF02875 Mur_ligase_C, 2 hits
PF08245 Mur_ligase_M, 2 hits
SUPFAMiSSF53244 SSF53244, 2 hits
SSF53623 SSF53623, 2 hits
SSF63418 SSF63418, 2 hits
TIGRFAMsiTIGR01085 murE, 1 hit
TIGR01143 murF, 1 hit

Sequencei

Sequence statusi: Complete.

F4EVD2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTLAELAAL VEGAEIKGDA AAKIADIVHD SREVTEGTLF VAIEGLHVDG
60 70 80 90 100
HSFIAQAVEK GACAILTERE IEIPEGAAAV LRVPNLAAAL EVIVPFFHDY
110 120 130 140 150
PSRKMRIIGI TGTNGKTTTS YMTRAILREA GYKVGLIGTI QILVEDEALP
160 170 180 190 200
IHNTTPDVVV LERTLAYMAR RGMDFVVMEV SSHALDQNRV AGIEFDTAVF
210 220 230 240 250
TNLTQDHLDY HKTLENYKRA KARLFDLVSR KGAKEGKTAV VNDDDAAGET
260 270 280 290 300
MLAHAKCAHL TYAVKKEAAL RAENIRVHAR GLELTLAGAF GRMDLSLGVT
310 320 330 340 350
GIFNVYNVMS AVGAALAERI APEAIKRALE AFKSVPGRFE LVDAGQAFSI
360 370 380 390 400
IVDYAHTPDG LENILNTARE IAAGRILTVF GCGGDRDRTK RPIMGRIAAA
410 420 430 440 450
LSDVVLVTSD NPRTEDPARI LDEVEVGVLE KIGDKCHEKI ADRRTAIVRA
460 470 480 490 500
VALAEKDDIV IIAGKGHENY QILKDKTIHF DDKEVAREAV AAREKSYAMH
510 520 530 540 550
FSLADIERAA KAEVVRTAAD IAFSDIVTDT RKIAAGSLFV ALQGERFDGA
560 570 580 590 600
DFAAQAVEKG AAGVLVAVDT PEAKLPKTGV VLKAKDTLLA YQQIAAAWRR
610 620 630 640 650
KFSIPVVAIT GSNGKTTTKD LTAAVLGACL LVQKTAANYN NEIGLPLTLL
660 670 680 690 700
GLRAAHEAAV VEIGMRGLGQ IAALAPLAAP TVAIVTNVGE VHMELLGSIE
710 720 730 740 750
NIARAKAELV EAVEPGGTVI LNADDARVLA MREKAKAGVR VVTFGLSRDA
760 770 780 790 800
DVRAVAVGKA EGGMRFMLEW KNERGRLERH ELFLPMPGRH NVSNALAAIA
810 820 830 840 850
AARVLGLSLA DVRRGLAVPP AQKMRFAVEK CGAYTFVNDA YNASPVSTRA
860 870 880 890 900
SLKTLAEMFA GRKIAVLGDM LELGSASKSG HASVGEEAAH LGFAAVLSRG
910 920 930 940 950
EESRFIAEAA KAGGVPLVER MTSHEAAAAR LKEILQPGDA VLFKGSRGMK
960
MDEIIPLLKK ALEAGK
Length:966
Mass (Da):103,109
Last modified:June 28, 2011 - v1
Checksum:iA652F89D4169576A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002637 Genomic DNA Translation: AEC00778.1
RefSeqiWP_013740969.1, NZ_GG698596.1

Genome annotation databases

EnsemblBacteriaiAEC00778; AEC00778; Selsp_1823
KEGGissg:Selsp_1823

Similar proteinsi

Entry informationi

Entry nameiF4EVD2_SELS3
AccessioniPrimary (citable) accession number: F4EVD2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 18, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again