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Entry version 46 (10 Feb 2021)
Sequence version 1 (31 May 2011)
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Protein

CRISPR-associated nuclease/helicase Cas3

Gene

cas3

Organism
Streptococcus thermophilus
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Functions as a ssDNA-dependent ATPase; dsDNA, ssRNA do not stimulate ATPase activity, while other nucleotides (aside from dATP) are not hydrolyzed. Functions as a ssDNA nuclease; activity does not require ATP. Functions as an ATP-dependent helicase; helicase activity requires hydrolysable ATP. Unwinds both DNA/DNA hybrids and RNA/DNA hybrids, moving mostly in a 3' to 5' direction.

1 Publication

Miscellaneous

Found in the E.coli-type CRISPR4/cas system.

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mn2+, Mg2+, Ca2+Note: Mn2+ or Mg2+ or Ca2+. ATPase and nuclease activities are dependent on divalent cations, for ATPase Mn2+ is marginally preferred over Mg2+ or Ca2+.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Nuclease activity is inhibited by EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi77MagnesiumBy similarity1
Metal bindingi150MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi310 – 317ATPPROSITE-ProRule annotation8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, Nuclease
Biological processAntiviral defense
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
CRISPR-associated nuclease/helicase Cas3 (EC:3.1.-.-, EC:3.6.4.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:cas3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStreptococcus thermophilus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi77D → A: Loss of ssDNA nuclease activity, no effect on helicase activity. 1 Publication1
Mutagenesisi227D → A: Loss of ssDNA nuclease activity. 1 Publication1
Mutagenesisi290Q → A: Loss of ssDNA-dependent ATPase. 1 Publication1
Mutagenesisi316K → A: Loss of ssDNA-dependent ATPase. 1 Publication1
Mutagenesisi452D → A: Loss of ssDNA-dependent ATPase, loss of helicase activity. 1 Publication1
Mutagenesisi453E → A: Loss of ssDNA-dependent ATPase. 1 Publication1
Mutagenesisi663R → A: Loss of ssDNA-dependent ATPase. 1 Publication1
Mutagenesisi666R → A: Loss of ssDNA-dependent ATPase. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004176071 – 926CRISPR-associated nuclease/helicase Cas3Add BLAST926

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
F2XG53

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini17 – 229HD Cas3-typePROSITE-ProRule annotationAdd BLAST213
Domaini297 – 504Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST208
Domaini551 – 745Helicase C-terminalPROSITE-ProRule annotationAdd BLAST195

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi452 – 455DEAH box4

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Proteins of this family have an N-terminal nuclease domain and a C-terminal helicase/ATPase domain. In some CRISPR/Cas systems the domains are swapped, in others they are encoded separately.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.Curated
In the central section; belongs to the CRISPR-associated helicase Cas3 family.Curated

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.3210.30, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR035011, Cas3
IPR041372, Cas3_C
IPR006483, CRISPR-assoc_Cas3_HD
IPR038257, CRISPR-assoc_Cas3_HD_sf
IPR011545, DEAD/DEAH_box_helicase_dom
IPR014001, Helicase_ATP-bd
IPR001650, Helicase_C
IPR006474, Helicase_Cas3_CRISPR-ass_core
IPR027417, P-loop_NTPase

The PANTHER Classification System

More...
PANTHERi
PTHR47959:SF5, PTHR47959:SF5, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF18395, Cas3_C, 1 hit
PF00270, DEAD, 1 hit
PF18019, HD_6, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00487, DEXDc, 1 hit
SM00490, HELICc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52540, SSF52540, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01587, cas3_core, 1 hit
TIGR01596, cas3_HD, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51643, HD_CAS3, 1 hit
PS51192, HELICASE_ATP_BIND_1, 1 hit
PS51194, HELICASE_CTER, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

F2XG53-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKHINDYFWA KKTEENSRLL WLPLTQHLED TKNIAGLLWE HWLSEGQKVL
60 70 80 90 100
IENSINVKSN IENQGKRLAQ FLGAVHDIGK ATPAFQTQKG YANSVDLDIQ
110 120 130 140 150
LLEKLERAGF SGISSLQLAS PKKSHHSIAG QYLLSHYGVD EDIATIIGGH
160 170 180 190 200
HGRPVDDLDG LNSQKSYPSN YYQDEKKDSL VYQKWKSNQE AFLNWALTET
210 220 230 240 250
GFNSVSQLPK IKQPAQVILS GLLIMSDWIA SNEHFFPLLS LDETDVKNKS
260 270 280 290 300
QRIETGFKKW KKSNLWQPET FVDLVTLYQE RFGFSPRNFQ LILSQTIEKT
310 320 330 340 350
TNPGIVILEA PMGIGKTEAA LAVSEQLSSK KGCSGLFFGL PTQATSNGIF
360 370 380 390 400
KRIEQWTENI KGNNSDHFSI QLVHGKAALN TDFIELLKGN TINMDDSENG
410 420 430 440 450
SIFVNEWFSG RKTSALDDFV VGTVDQFLMV ALKQKHLALR HLGFSKKVIV
460 470 480 490 500
IDEVHAYDAY MSQYLLEAIR WMGAYGVPVI ILSATLPAQQ REKLIKSYMA
510 520 530 540 550
GMGVKWRDIE NIDQIKIDAY PLITYNDGPD IHQVKMFEKQ EQKNIYIHRL
560 570 580 590 600
PEEQLFDIVK EGLDNGGVVG IIVNTVRKSQ ELARNFSDIF GDDMVDLLHS
610 620 630 640 650
NFIATERIRK EKDLLQEIGK KAIRPPKKII IGTQVLEQSL DIDFDVLISD
660 670 680 690 700
LAPMDLLIQR IGRLHRHKIK RPQKHEVARF YVLGTFEEFD FDEGTRLVYG
710 720 730 740 750
DYLLARTQYF LPDKIRLPDD ISPLVQKVYN SDLTITFPKP ELHKKYLDAK
760 770 780 790 800
IEHDDKIKNK ETKAKSYRIA NPVLKKSRVR TNSLIGWLKN LHPNDSEEKA
810 820 830 840 850
YAQVRDIEDT VEVIALKKIS DGYGLFIENK DISQNITDPI IAKKVAQNTL
860 870 880 890 900
RLPMSLSKAY NIDQTINELE RYNNSHLSQW QNSSWLKGSL GIIFDKNNEF
910 920
ILNGFKLLYD EKYGVTIERL DKNESV
Length:926
Mass (Da):105,760
Last modified:May 31, 2011 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCFBD0C7E1300FEF3
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
HQ453272 Genomic DNA Translation: ADV90802.1

NCBI Reference Sequences

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RefSeqi
WP_037623090.1, NZ_QFLC01000003.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ453272 Genomic DNA Translation: ADV90802.1
RefSeqiWP_037623090.1, NZ_QFLC01000003.1

3D structure databases

SMRiF2XG53
ModBaseiSearch...

Family and domain databases

Gene3Di1.10.3210.30, 1 hit
InterProiView protein in InterPro
IPR035011, Cas3
IPR041372, Cas3_C
IPR006483, CRISPR-assoc_Cas3_HD
IPR038257, CRISPR-assoc_Cas3_HD_sf
IPR011545, DEAD/DEAH_box_helicase_dom
IPR014001, Helicase_ATP-bd
IPR001650, Helicase_C
IPR006474, Helicase_Cas3_CRISPR-ass_core
IPR027417, P-loop_NTPase
PANTHERiPTHR47959:SF5, PTHR47959:SF5, 1 hit
PfamiView protein in Pfam
PF18395, Cas3_C, 1 hit
PF00270, DEAD, 1 hit
PF18019, HD_6, 1 hit
SMARTiView protein in SMART
SM00487, DEXDc, 1 hit
SM00490, HELICc, 1 hit
SUPFAMiSSF52540, SSF52540, 1 hit
TIGRFAMsiTIGR01587, cas3_core, 1 hit
TIGR01596, cas3_HD, 1 hit
PROSITEiView protein in PROSITE
PS51643, HD_CAS3, 1 hit
PS51192, HELICASE_ATP_BIND_1, 1 hit
PS51194, HELICASE_CTER, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiCAS3_STRTR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: F2XG53
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: May 31, 2011
Last modified: February 10, 2021
This is version 46 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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