UniProtKB - F2W8B0 (F2W8B0_RAT)
Protein
Catechol O-methyltransferase
Gene
Comt
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones.UniRule annotation
Catalytic activityi
- 2-hydroxy-17β-estradiol + S-adenosyl-L-methionine = 2-hydroxy-3-methoxy-17β-estradiol + H+ + S-adenosyl-L-homocysteineARBA annotationThis reaction proceeds in the forwardARBA annotation direction.
- 2-hydroxy-17β-estradiol + S-adenosyl-L-methionine = 2-methoxy-17β-estradiol + H+ + S-adenosyl-L-homocysteineARBA annotationThis reaction proceeds in the forwardARBA annotation direction.
- 2-hydroxyestrone + S-adenosyl-L-methionine = 2-hydroxy-3-methoxy-estrone + H+ + S-adenosyl-L-homocysteineARBA annotationThis reaction proceeds in the forwardARBA annotation direction.
- 2-hydroxyestrone + S-adenosyl-L-methionine = 2-methoxyestrone + H+ + S-adenosyl-L-homocysteineARBA annotationThis reaction proceeds in the forwardARBA annotation direction.
- 4-hydroxy-17β-estradiol + S-adenosyl-L-methionine = 4-methoxy-17β-estradiol + H+ + S-adenosyl-L-homocysteineARBA annotationThis reaction proceeds in the forwardARBA annotation direction.
- 4-hydroxyestrone + S-adenosyl-L-methionine = 4-methoxyestrone + H+ + S-adenosyl-L-homocysteineARBA annotationThis reaction proceeds in the forwardARBA annotation direction.
- a catechol + S-adenosyl-L-methionine = a guaiacol + H+ + S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.6UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 85 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 115 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 133 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Metal bindingi | 184 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 184 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 187 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 212 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 213 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 213 | SubstrateUniRule annotation | 1 | |
Binding sitei | 242 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- catechol O-methyltransferase activity Source: UniProtKB-UniRule
- L-dopa O-methyltransferase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- orcinol O-methyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- cellular response to phosphate starvation Source: Ensembl
- dopamine catabolic process Source: Ensembl
- methylation Source: UniProtKB-KW
- neurotransmitter catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | MethyltransferaseUniRule annotationImportedARBA annotation, Transferase |
Biological process | Catecholamine metabolismUniRule annotationARBA annotation, Neurotransmitter degradationUniRule annotationARBA annotation |
Ligand | MagnesiumUniRule annotation, Metal-bindingUniRule annotation, S-adenosyl-L-methionineUniRule annotationARBA annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Catechol O-methyltransferaseUniRule annotationARBA annotation (EC:2.1.1.6UniRule annotationARBA annotation) |
Gene namesi | Name:ComtImported ORF Names:rCG_36721Imported |
Organismi | Rattus norvegicus (Rat)Imported |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Organism-specific databases
RGDi | 2379, Comt |
Subcellular locationi
Plasma membrane
- Cell membrane ARBA annotation; Single-pass type II membrane protein ARBA annotation; Extracellular side ARBA annotation
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000001889, Expressed in liver and 20 other tissues |
ExpressionAtlasi | F2W8B0, baseline and differential |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 160 – 163 | S-adenosyl-L-methionine bindingUniRule annotation | 4 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.UniRule annotationARBA annotation
Phylogenomic databases
HOGENOMi | CLU_050461_5_0_1 |
OMAi | CTHYSSY |
OrthoDBi | 1274244at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR017128, Catechol_O-MeTrfase_euk IPR029063, SAM-dependent_MTases IPR002935, SAM_O-MeTrfase |
Pfami | View protein in Pfam PF01596, Methyltransf_3, 1 hit |
PIRSFi | PIRSF037177, Catechol_O-mtfrase_euk, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51682, SAM_OMT_I, 1 hit |
i Sequence
Sequence statusi: Complete.
F2W8B0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPLAAVSLGL LLLALLLLLR HLGWGLVTIF WFEYVLQPVH NLIMGDTKEQ
60 70 80 90 100
RILRYVQQNA KPGDPQSVLE AIDTYCTQKE WAMNVGDAKG QIMDAVIREY
110 120 130 140 150
SPSLVLELGA YCGYSAVRMA RLLQPGARLL TMEMNPDYAA ITQQMLNFAG
160 170 180 190 200
LQDKVTILNG ASQDLIPQLK KKYDVDTLDM VFLDHWKDRY LPDTLLLEKC
210 220 230 240 250
GLLRKGTVLL ADNVIVPGTP DFLAYVRGSS SFECTHYSSY LEYMKVVDGL
260
EKAIYQGPSS PDKS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM443074 Genomic DNA Translation: AEA41111.1 HM443075 Genomic DNA Translation: AEA41112.1 CH473999 Genomic DNA Translation: EDL77940.1 |
RefSeqi | NP_036663.1, NM_012531.2 |
Genome annotation databases
GeneIDi | 24267 |
KEGGi | rno:24267 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | HM443074 Genomic DNA Translation: AEA41111.1 HM443075 Genomic DNA Translation: AEA41112.1 CH473999 Genomic DNA Translation: EDL77940.1 |
RefSeqi | NP_036663.1, NM_012531.2 |
3D structure databases
SMRi | F2W8B0 |
ModBasei | Search... |
Chemistry databases
BindingDBi | F2W8B0 |
Genome annotation databases
GeneIDi | 24267 |
KEGGi | rno:24267 |
Organism-specific databases
CTDi | 1312 |
RGDi | 2379, Comt |
Phylogenomic databases
HOGENOMi | CLU_050461_5_0_1 |
OMAi | CTHYSSY |
OrthoDBi | 1274244at2759 |
Gene expression databases
Bgeei | ENSRNOG00000001889, Expressed in liver and 20 other tissues |
ExpressionAtlasi | F2W8B0, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR017128, Catechol_O-MeTrfase_euk IPR029063, SAM-dependent_MTases IPR002935, SAM_O-MeTrfase |
Pfami | View protein in Pfam PF01596, Methyltransf_3, 1 hit |
PIRSFi | PIRSF037177, Catechol_O-mtfrase_euk, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51682, SAM_OMT_I, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F2W8B0_RAT | |
Accessioni | F2W8B0Primary (citable) accession number: F2W8B0 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 31, 2011 |
Last sequence update: | May 31, 2011 | |
Last modified: | February 10, 2021 | |
This is version 80 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |