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Protein

Lipoyl synthase, mitochondrial

Gene

BDDG_01811

Organism
Ajellomyces dermatitidis (strain ATCC 18188 / CBS 674.68) (Blastomyces dermatitidis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (BDDG_01836)
  2. Lipoyl synthase, mitochondrial (BDDG_01811)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi141Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi146Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi152Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi172Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi176Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi179Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:BDDG_01811Imported
OrganismiAjellomyces dermatitidis (strain ATCC 18188 / CBS 674.68) (Blastomyces dermatitidis)Imported
Taxonomic identifieri653446 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesAjellomycetaceaeBlastomyces
Proteomesi
  • UP000007802 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliF2T6R0
SMRiF2T6R0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 371Elp3InterPro annotationAdd BLAST210

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

OrthoDBiEOG092C13O2

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

F2T6R0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAASTGKLRT LFSAHSSLSA RPSSALPALR LTILRSYATT TPPDSSISDP
60 70 80 90 100
SNSSTTVKRP PTAFKDKLNA GPAFSDFVSG KKDEPLDPAE AYALKTALVG
110 120 130 140 150
PAGRKKEITR LPSWLKTPIP DSSNYKRIKN DLRGLNLHTV CEEARCPNIS
160 170 180 190 200
DCWGGSSKSA ATATIMLMGD TCTRGCRFCS VKTSNKPPPL DPHEPENTAE
210 220 230 240 250
ALSRWGLGYV VLTSVDRDDL ADGGARHFAE TVLKIKQKAP NILVECLTGD
260 270 280 290 300
YAGDLEMVAL VANSGLDVYA HNVETVEALT PFVRDRRATF QQSLRVLKAA
310 320 330 340 350
KATKPELITK TSLMLGLGET EAQLWDTLRA LRAIDVDVVT FGQYMRPTKR
360 370 380 390 400
HMAVHEYVRP DVFDMWKERA LEMGFLYCAS GPLVRSSYKA GEAFIENVLK
410 420 430
KKRGKNVGSA SGKGTTSENV EKLVAGEAVR
Length:430
Mass (Da):46,750
Last modified:May 31, 2011 - v1
Checksum:i920ED93E44FE430D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG749411 Genomic DNA Translation: EGE78874.1

Genome annotation databases

EnsemblFungiiEGE78874; EGE78874; BDDG_01811

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GG749411 Genomic DNA Translation: EGE78874.1

3D structure databases

ProteinModelPortaliF2T6R0
SMRiF2T6R0
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEGE78874; EGE78874; BDDG_01811

Phylogenomic databases

OrthoDBiEOG092C13O2

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PANTHERiPTHR10949 PTHR10949, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiF2T6R0_AJEDA
AccessioniPrimary (citable) accession number: F2T6R0
Entry historyiIntegrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: October 10, 2018
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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