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Protein

Translation factor GUF1 homolog, chloroplastic

Gene
N/A
Organism
Hordeum vulgare subsp. vulgare (Domesticated barley)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Promotes chloroplast protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 91GTPUniRule annotation8
Nucleotide bindingi149 – 153GTPUniRule annotation5
Nucleotide bindingi203 – 206GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation
Biological processProtein biosynthesisUniRule annotation
LigandGTP-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation factor GUF1 homolog, chloroplasticUniRule annotation (EC:3.6.5.n1UniRule annotation)
Alternative name(s):
Elongation factor 4 homologUniRule annotation
Short name:
EF-4UniRule annotation
GTPase GUF1 homologUniRule annotation
Ribosomal back-translocaseUniRule annotation
OrganismiHordeum vulgare subsp. vulgare (Domesticated barley)Imported
Taxonomic identifieri112509 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

ChloroplastUniRule annotation, Plastid

Expressioni

Gene expression databases

ExpressionAtlasiF2CRM7 baseline and differential

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 256Tr-type GInterPro annotationAdd BLAST182

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_03138 GUFP, 1 hit
MF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027518 GUFP
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

F2CRM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTAVCRL AVLTPAPSPS PLASGRRRPA APFSATSRPR ALSAASRGRV
60 70 80 90 100
LCLAASAPAS STDAGQDRLQ KVPITNIRNF CIIAHIDHGK STLADKLLEM
110 120 130 140 150
TGTVQKRDMK QQFLDNMDLE RERGITIKLQ AARMRYVMNN EPYCLNLIDT
160 170 180 190 200
PGHVDFSYEV SRSLAACEGA LLVVDASQGV EAQTLANVYL ALESNLEIIP
210 220 230 240 250
VLNKIDLPGA EPDRIAQEIE EIIGLDCSNA IRCSAKEGIG ITEILDAIVT
260 270 280 290 300
KVPPPKDTLK NPLRALIFDS YYDAYRGVIV YFRVIDGSIR KGDKICFMAN
310 320 330 340 350
KKEYVADEIG VLSPNQMEAD ELHAGEVGYL SASIRSVADA RVGDTITHFA
360 370 380 390 400
KRAESALPGY SEATPMVFCG LFPLEADQFE ELREALGRLQ LNDAALKFEP
410 420 430 440 450
ESSSAMGFGF RCGFLGLLHM EIVQERLERE YNLNLIITAP SVVYRVNCSN
460 470 480 490 500
NETVECSNPS LLPEPGKRRS IEEPYVKIEL LTPKDYIGPI MELGQERRGE
510 520 530 540 550
FKEMNYITEN RAKLTYMLPL AEMVGDFFDQ LKSRSKGYAS MEYSVVGYRV
560 570 580 590 600
SDLVKLDIQI NGEPVEALST IVHREKAYAV GRALTQKLKE LIPRQMFKIP
610 620 630 640 650
IQACIGAKVI ASEALSAIRK DVLSKCYGGD ISRKKKLLKK QAEGKKRMKA
660 670
IGRVEVPQEA FMAVLKLEKE VL
Length:672
Mass (Da):74,295
Last modified:May 31, 2011 - v1
Checksum:i11DE0677A9B52972
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK354279 mRNA Translation: BAJ85498.1
AK365900 mRNA Translation: BAJ97103.1

Similar proteinsi

Entry informationi

Entry nameiF2CRM7_HORVV
AccessioniPrimary (citable) accession number: F2CRM7
Entry historyiIntegrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: May 23, 2018
This is version 49 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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