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Entry version 59 (26 Feb 2020)
Sequence version 1 (03 May 2011)
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Protein

F(420)H(2) dehydrogenase subunit L

Gene

fpoL

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Component of the F(420)H2 dehydrogenase (FPO complex) which is part of the energy-conserving F(420)H2:heterodisulfide oxidoreductase system. The membrane-bound electron transfer system of the complex plays an important role in the metabolism of methylotrophic methanogens when the organisms grow on methanol or methylamines. Catalyzes the oxidation of methanophenazine to dihydromethanophenazine. It shuttles electrons from F(420)H2, via FAD and iron-sulfur (Fe-S) centers, to methanophenazine (an electron carrier in the membrane). It couples the redox reaction to proton translocation (for every two electrons transferred, two hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. It also catalyzes the oxidation of F(420)H2 with quinones such as 2,3-dimethyl-1,4-naphthoquinone, 2-methyl-1,4-naphthoquinone and tetramethyl-p-benzoquinone.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Measured for the whole complex.
  1. KM=7 µM for F(420)H2 (at 37 degrees Celsius and pH 7)1 Publication
  1. Vmax=17 µmol/min/mg enzyme (at 37 degrees Celsius and pH 7)1 Publication

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 39 degrees Celsius.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Methanogenesis, Methanol utilization, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MMAZ192952:G1FZI-2587-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.12.98.3 3270

Protein family/group databases

Transport Classification Database

More...
TCDBi
3.D.9.1.1 the h(+)-translocating f420h2 dehydrogenase (f420h2dh) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
F(420)H(2) dehydrogenase subunit L (EC:1.12.98.3)
Alternative name(s):
F(420)H(2)-dependent phenazine dehydrogenase subunit L
F(420)H(2)-dependent phenazine oxidoreductase subunit L
Short name:
FPO subunit L
Methanophenazine hydrogenase subunit L
Methanosarcina-phenazine hydrogenase subunit L
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:fpoL
Ordered Locus Names:MM_2482
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri192952 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaStenosarchaea groupMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000595 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Transmembranei79 – 99HelicalSequence analysisAdd BLAST21
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Transmembranei179 – 199HelicalSequence analysisAdd BLAST21
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Transmembranei337 – 357HelicalSequence analysisAdd BLAST21
Transmembranei360 – 380HelicalSequence analysisAdd BLAST21
Transmembranei394 – 414HelicalSequence analysisAdd BLAST21
Transmembranei447 – 467HelicalSequence analysisAdd BLAST21
Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
Transmembranei545 – 565HelicalSequence analysisAdd BLAST21
Transmembranei601 – 621HelicalSequence analysisAdd BLAST21
Transmembranei652 – 672HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004239571 – 672F(420)H(2) dehydrogenase subunit LAdd BLAST672

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
F1SVK0

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

The FPO complex is composed of at least 13 different subunits. FpoA, FpoH, FpoJ, FpoK, FpoL, FpoM and FpoN proteins constitute the membrane sector of the complex.

1 Publication

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
192952.MM_2482

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
arCOG01539 Archaea
COG1009 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_007100_6_0_2

KEGG Orthology (KO)

More...
KOi
K22167

Identification of Orthologs from Complete Genome Data

More...
OMAi
MTHAYFK

Database of Orthologous Groups

More...
OrthoDBi
28394at2157

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003945 NADHpl_OxRdtase_5
IPR018393 NADHpl_OxRdtase_5_subgr
IPR001750 ND/Mrp_mem
IPR001516 Proton_antipo_N

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00361 Proton_antipo_M, 1 hit
PF00662 Proton_antipo_N, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01435 NPOXDRDTASE5

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01974 NDH_I_L, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

F1SVK0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKTALEEFA FLIPLLPALA FAITFFFGRK MPSGGAIVPI LAIAASFVIS
60 70 80 90 100
FAITLGLLAN PEEVISQSYS WFAVLNIGIL IDPLAAVMLS MVSFVSLLIH
110 120 130 140 150
IYAVSYMSHD AGKARYFAET ALFTAAMLSL VLSDNILQLF VSWELVGLCS
160 170 180 190 200
YLLIGFWFEK PSAAAAAKKA FLTTRIGDVM FLTGIIVLTS DLLKVSGGFQ
210 220 230 240 250
DGVYLLRFDE IFSYIPELAA LQINILGFEI SHLTIITLLF FGGAVGKSGQ
260 270 280 290 300
FPLHVWLPDA MEGPTTVSAL IHAATMVTAG VYLVARTFPM FIAAPDSLMV
310 320 330 340 350
VAYFGGFTAL FAGTMGIVMN DLKRVLAFST ISQLGYMMLG LGLGTAIGLE
360 370 380 390 400
AVGISLFHLI NHAFFKALLF LCAGSVIHAV GTQDMRELGG VGKVMPITAA
410 420 430 440 450
TMTIAALALA GFGIPGTSIG TSGFMSKDPI IEAAYLFGEH SSNWIPYVFS
460 470 480 490 500
ILAALLTSIY IFRLIFMTFT GKPRSNYHGH ESPAIMTIPL SILAIFALAF
510 520 530 540 550
GALTRTGFME FLEETFTNSF VNLDIGALAG IGENELVAAA GHEPLAVLWP
560 570 580 590 600
PVIVALAGFA IAFVIYYLRA FSLGPLASMK NPIYRLLYNR YYQHQIYTEF
610 620 630 640 650
FSIGIVYGII AFLTQVVDVI IDSVVEGIGI VTVFVGEELR KIQTGVVQTY
660 670
ATALIAGVSL LIILVKLIME VL
Length:672
Mass (Da):72,393
Last modified:May 3, 2011 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4752698BECC3E50E
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF228525 Genomic DNA Translation: AAF65739.1
AE008384 Genomic DNA Translation: AAM32178.1

NCBI Reference Sequences

More...
RefSeqi
WP_011034400.1, NC_003901.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAM32178; AAM32178; MM_2482

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
24882205

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mma:MM_2482

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|192952.21.peg.2840

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228525 Genomic DNA Translation: AAF65739.1
AE008384 Genomic DNA Translation: AAM32178.1
RefSeqiWP_011034400.1, NC_003901.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi192952.MM_2482

Protein family/group databases

TCDBi3.D.9.1.1 the h(+)-translocating f420h2 dehydrogenase (f420h2dh) family

Proteomic databases

PRIDEiF1SVK0

Genome annotation databases

EnsemblBacteriaiAAM32178; AAM32178; MM_2482
GeneIDi24882205
KEGGimma:MM_2482
PATRICifig|192952.21.peg.2840

Phylogenomic databases

eggNOGiarCOG01539 Archaea
COG1009 LUCA
HOGENOMiCLU_007100_6_0_2
KOiK22167
OMAiMTHAYFK
OrthoDBi28394at2157

Enzyme and pathway databases

BioCyciMMAZ192952:G1FZI-2587-MONOMER
BRENDAi1.12.98.3 3270

Family and domain databases

InterProiView protein in InterPro
IPR003945 NADHpl_OxRdtase_5
IPR018393 NADHpl_OxRdtase_5_subgr
IPR001750 ND/Mrp_mem
IPR001516 Proton_antipo_N
PfamiView protein in Pfam
PF00361 Proton_antipo_M, 1 hit
PF00662 Proton_antipo_N, 1 hit
PRINTSiPR01435 NPOXDRDTASE5
TIGRFAMsiTIGR01974 NDH_I_L, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiFPOL_METMA
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: F1SVK0
Secondary accession number(s): Q7LWJ9, Q9P9F5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: May 3, 2011
Last modified: February 26, 2020
This is version 59 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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