UniProtKB - F1RKQ4 (TKFC_PIG)
Protein
Triokinase/FMN cyclase
Gene
TKFC
Organism
Sus scrofa (Pig)
Status
Functioni
Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate (PubMed:7831203). Represses IFIH1-mediated cellular antiviral response (By similarity).By similarity1 Publication
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- Mg2+By similarity
- Mn2+By similarity, Co2+By similarityNote: Manganese or cobalt are requested for FAD-AMP lyase activity.By similarity
Kineticsi
- KM=11 µM for D-glyceraldehyde1 Publication
- KM=5 µM for dihydroxyacetone1 Publication
pH dependencei
Optimum pH is 7.1 Publication
Temperature dependencei
Optimum temperature is 60 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 109 | DihydroxyacetonePROSITE-ProRule annotation | 1 | |
Binding sitei | 114 | DihydroxyacetonePROSITE-ProRule annotation | 1 | |
Active sitei | 221 | Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation | 1 | |
Binding sitei | 486 | ATP; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 401 – 404 | ATPBy similarity | 4 | |
Nucleotide bindingi | 446 – 447 | ATPBy similarity | 2 | |
Nucleotide bindingi | 494 – 495 | ATPBy similarity | 2 | |
Nucleotide bindingi | 557 – 559 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- FAD-AMP lyase (cyclizing) activity Source: UniProtKB-EC
- glycerone kinase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- triokinase activity Source: UniProtKB-EC
GO - Biological processi
- glycerol catabolic process Source: GO_Central
- negative regulation of MDA-5 signaling pathway Source: UniProtKB
Keywordsi
Molecular function | Kinase, Lyase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Cobalt, FAD, Flavoprotein, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Triokinase/FMN cyclaseAlternative name(s): Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) Including the following 2 domains: |
Gene namesi | Name:TKFC Synonyms:DAK |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418834 | 1 – 579 | Triokinase/FMN cyclaseAdd BLAST | 579 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 350 | PhosphoserineBy similarity | 1 | |
Modified residuei | 511 | PhosphoserineBy similarity | 1 | |
Modified residuei | 546 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | F1RKQ4 |
PeptideAtlasi | F1RKQ4 |
PRIDEi | F1RKQ4 |
Expressioni
Tissue specificityi
Detected in kidney (at protein level).1 Publication
Gene expression databases
Bgeei | ENSSSCG00000013087, Expressed in liver and 39 other tissues |
Genevisiblei | F1RKQ4, SS |
Interactioni
Subunit structurei
Homodimer (PubMed:7831203).
Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection (By similarity).
By similarity1 PublicationProtein-protein interaction databases
STRINGi | 9823.ENSSSCP00000013919 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 336 | DhaKPROSITE-ProRule annotationAdd BLAST | 328 | |
Domaini | 372 – 572 | DhaLPROSITE-ProRule annotationAdd BLAST | 201 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 59 | Dihydroxyacetone bindingBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 457 – 569 | Ala-richAdd BLAST | 113 |
Domaini
DhaK and DhaL domains have differential roles, individually DhaK is inactive and DhaL displays cyclase but not kinase activity.By similarity
Sequence similaritiesi
Belongs to the dihydroxyacetone kinase (DAK) family.Curated
Phylogenomic databases
eggNOGi | KOG2426, Eukaryota |
HOGENOMi | CLU_017054_6_2_1 |
InParanoidi | F1RKQ4 |
OrthoDBi | 472175at2759 |
TreeFami | TF313821 |
Family and domain databases
Gene3Di | 1.25.40.340, 1 hit |
InterProi | View protein in InterPro IPR012734, DhaK_ATP IPR004006, DhaK_dom IPR004007, DhaL_dom IPR036117, DhaL_dom_sf |
Pfami | View protein in Pfam PF02733, Dak1, 1 hit PF02734, Dak2, 1 hit |
SMARTi | View protein in SMART SM01120, Dak2, 1 hit |
SUPFAMi | SSF101473, SSF101473, 1 hit |
TIGRFAMsi | TIGR02361, dak_ATP, 1 hit |
PROSITEi | View protein in PROSITE PS51481, DHAK, 1 hit PS51480, DHAL, 1 hit |
i Sequence
Sequence statusi: Complete.
F1RKQ4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTSKKLVNSV AGCADDALAG LVACNPSLQL LQGHRVALRS DLDSLKGRVA
60 70 80 90 100
LLSGGGSGHE PAHAGFIGKG MLTGVIAGAV FTSPAVGSIL AAIRAVAQAG
110 120 130 140 150
TVGTLLIVKN YTGDRLNFGL AREQARAEGI PVEMVVVGDD SAFTVLKKAG
160 170 180 190 200
RRGLCGTVLI HKVAGALAEA GVGLEEITNR VSVVAKAMGT LGVSLSSCSV
210 220 230 240 250
PGSRPTFELS ADEVELGLGI HGEAGVLRIK MATADEIVAH MLNHMTDSSN
260 270 280 290 300
VSHVPVQSGS SVVLMVNNLG GLSYLELGII ADAAVRFLEG RGVKIARALV
310 320 330 340 350
GTFMSALEMP GVSLTLLLVD EPLLKLIDAE TTAAAWPNVA KVSVTGRKRS
360 370 380 390 400
RAAPAEPPEA PDATAAGGAT SKQMVRVLER VCTTLLGLED QLNALDRAAG
410 420 430 440 450
DGDCGTTHSR AARAIQGWLK ESPPPASPAQ LLSKLSLLLL EKMGGSSGAL
460 470 480 490 500
YGLFLTAAAQ PLKAKTDLAA WSAAMDAGLE AMQKYGKAAP GDRTMLDSLW
510 520 530 540 550
AAGQELQAWK SPGANLLPVL TKALLENAEA AAEATKNMEA GAGRASYISS
560 570
ARLDQPDPGA VAAAAILRAI LEVLQSQGA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU462918 Genomic DNA No translation available. CU896534 Genomic DNA No translation available. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU462918 Genomic DNA No translation available. CU896534 Genomic DNA No translation available. |
3D structure databases
SMRi | F1RKQ4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000013919 |
Proteomic databases
PaxDbi | F1RKQ4 |
PeptideAtlasi | F1RKQ4 |
PRIDEi | F1RKQ4 |
Phylogenomic databases
eggNOGi | KOG2426, Eukaryota |
HOGENOMi | CLU_017054_6_2_1 |
InParanoidi | F1RKQ4 |
OrthoDBi | 472175at2759 |
TreeFami | TF313821 |
Gene expression databases
Bgeei | ENSSSCG00000013087, Expressed in liver and 39 other tissues |
Genevisiblei | F1RKQ4, SS |
Family and domain databases
Gene3Di | 1.25.40.340, 1 hit |
InterProi | View protein in InterPro IPR012734, DhaK_ATP IPR004006, DhaK_dom IPR004007, DhaL_dom IPR036117, DhaL_dom_sf |
Pfami | View protein in Pfam PF02733, Dak1, 1 hit PF02734, Dak2, 1 hit |
SMARTi | View protein in SMART SM01120, Dak2, 1 hit |
SUPFAMi | SSF101473, SSF101473, 1 hit |
TIGRFAMsi | TIGR02361, dak_ATP, 1 hit |
PROSITEi | View protein in PROSITE PS51481, DHAK, 1 hit PS51480, DHAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TKFC_PIG | |
Accessioni | F1RKQ4Primary (citable) accession number: F1RKQ4 Secondary accession number(s): Q9TR81 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | July 11, 2012 | |
Last modified: | December 2, 2020 | |
This is version 57 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families