UniProtKB - F1Q7F3 (F1Q7F3_DANRE)
Protein
NADPH--cytochrome P450 reductase
Gene
pora
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation
Caution
Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
Catalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 205 | FMNUniRule annotation | 1 | |
Binding sitei | 295 | NADPUniRule annotation | 1 | |
Binding sitei | 475 | FADUniRule annotation | 1 | |
Binding sitei | 531 | NADPUniRule annotation | 1 | |
Binding sitei | 635 | NADPUniRule annotation | 1 | |
Binding sitei | 673 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 83 – 88 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 135 – 138 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 170 – 179 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 451 – 454 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 469 – 471 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 485 – 488 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 592 – 593 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 598 – 602 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: GO_Central
- FMN binding Source: GO_Central
- NADP binding Source: UniProtKB-UniRule
- NADPH-hemoprotein reductase activity Source: GO_Central
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- response to hormone Source: GO_Central
Keywordsi
Molecular function | OxidoreductaseUniRule annotation |
Ligand | FADUniRule annotation, Flavoprotein, FMNUniRule annotation, NADPUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)Short name: CPRUniRule annotation Short name: P450RUniRule annotation |
Gene namesi | |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio)Imported |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-050809-121 pora |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 20 – 41 | HelicalUniRule annotationAdd BLAST | 22 |
Keywords - Cellular componenti
Endoplasmic reticulumUniRule annotation, MembraneExpressioni
Gene expression databases
Bgeei | ENSDARG00000055092 Expressed in 21 organ(s), highest expression level in head |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 77 – 221 | Flavodoxin-likeInterPro annotationAdd BLAST | 145 | |
Domaini | 276 – 517 | FAD-binding FR-typeInterPro annotationAdd BLAST | 242 |
Sequence similaritiesi
Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixUniRule annotationPhylogenomic databases
eggNOGi | KOG1158 Eukaryota COG0369 LUCA |
GeneTreei | ENSGT00940000156847 |
InParanoidi | F1Q7F3 |
OMAi | FPSCCCI |
TreeFami | TF105719 |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212 NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208 P450R, 1 hit |
PRINTSi | PR00369 FLAVODOXIN PR00371 FPNCR |
SUPFAMi | SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
F1Q7F3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDTEPQPVS DQDQTPDPLL SSLDIFLFSL IAGLLIYWFF FRKKVEPIPE
60 70 80 90 100
MKPFTLVTAP IRETSFIEKM KKTNRSVVVF YGSQTGTGEE FANRLSKDAH
110 120 130 140 150
RYGMKGMAAD PEEYDMSELS RLKEIPKSMA VFCMATYGEG DPTDNAQEFY
160 170 180 190 200
DWLQGTDDDL EGVNFAVFGL GNKTYEHYNA MGKYTDKRLA ELGGKRVFDL
210 220 230 240 250
GLGDDDSNLE EDFISWKEQF WPAVCEYFGV EATGEDSSIR QFELVVHNDI
260 270 280 290 300
NMNQVYTGEM GRLKSFQTQK PPFDAKNPFL ASVAVNRKLN KGGNRHLMHI
310 320 330 340 350
ELDITESKIR YDSGDHVAVY PTNDSAMVNR IGERLGVDLD AVISLKNLDE
360 370 380 390 400
ESNKKHPFPC PTTYRTALTH YLDINNMPRT NVLYELAQYA SDPQEQENMR
410 420 430 440 450
KMASASPEGK ALYQSWVLDS ERNILAILED LPSLNPPIDH LCELLPRLQA
460 470 480 490 500
RYYSIASSSK VHPHCIHICA VVIEYNTKTG RVFKGVATNW LKGKHVTDNG
510 520 530 540 550
HKPTVPMYVR RSQFRLPFKP SNPVIMIGPG TGIAPFMGFI QERAWQKEQG
560 570 580 590 600
KDVGETILYF GCRHSNEDFL YQQELEEFER AGVLTQLNVA FSRDQEQKVY
610 620 630 640 650
VQHLLKKNKQ QLWKLIHTDN AHIYVCGDAR NMARDVHAAF SEIAEQEGRL
660 670
THTQALDYFK KLMTKGRYSQ DVWS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR925774 Genomic DNA No translation available. |
Genome annotation databases
Ensembli | ENSDART00000077404; ENSDARP00000071871; ENSDARG00000055092 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CR925774 Genomic DNA No translation available. |
3D structure databases
ProteinModelPortali | F1Q7F3 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000071871 |
Proteomic databases
PaxDbi | F1Q7F3 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSDART00000077404; ENSDARP00000071871; ENSDARG00000055092 |
Organism-specific databases
ZFINi | ZDB-GENE-050809-121 pora |
Phylogenomic databases
eggNOGi | KOG1158 Eukaryota COG0369 LUCA |
GeneTreei | ENSGT00940000156847 |
InParanoidi | F1Q7F3 |
OMAi | FPSCCCI |
TreeFami | TF105719 |
Gene expression databases
Bgeei | ENSDARG00000055092 Expressed in 21 organ(s), highest expression level in head |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
HAMAPi | MF_03212 NCPR, 1 hit |
InterProi | View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit |
PIRSFi | PIRSF000208 P450R, 1 hit |
PRINTSi | PR00369 FLAVODOXIN PR00371 FPNCR |
SUPFAMi | SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | F1Q7F3_DANRE | |
Accessioni | F1Q7F3Primary (citable) accession number: F1Q7F3 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 3, 2011 |
Last sequence update: | May 3, 2011 | |
Last modified: | February 13, 2019 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |