UniProtKB - F1MSG6 (RPGF2_BOVIN)
Rap guanine nucleotide exchange factor 2
RAPGEF2
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 135 – 252 | cNMPAdd BLAST | 118 |
GO - Molecular functioni
- beta-1 adrenergic receptor binding Source: UniProtKB
- cAMP binding Source: UniProtKB
- GTPase activator activity Source: UniProtKB-KW
- PDZ domain binding Source: UniProtKB
- Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
- Ras guanyl-nucleotide exchange factor activity Source: UniProtKB
- WW domain binding Source: UniProtKB
GO - Biological processi
- adenylate cyclase-activating adrenergic receptor signaling pathway Source: UniProtKB
- blood vessel development Source: UniProtKB
- brain-derived neurotrophic factor receptor signaling pathway Source: UniProtKB
- cAMP-mediated signaling Source: UniProtKB
- cellular response to cAMP Source: UniProtKB
- cellular response to cGMP Source: UniProtKB
- cellular response to nerve growth factor stimulus Source: UniProtKB
- establishment of endothelial barrier Source: UniProtKB
- forebrain neuron development Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of dendrite morphogenesis Source: UniProtKB
- negative regulation of melanin biosynthetic process Source: UniProtKB
- nerve growth factor signaling pathway Source: UniProtKB
- neuron migration Source: UniProtKB
- neuron projection development Source: UniProtKB
- neuropeptide signaling pathway Source: UniProtKB
- positive regulation of cAMP-dependent protein kinase activity Source: UniProtKB
- positive regulation of cAMP-mediated signaling Source: UniProtKB
- positive regulation of dendritic cell apoptotic process Source: UniProtKB
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of neuron migration Source: UniProtKB
- positive regulation of neuron projection development Source: UniProtKB
- positive regulation of protein binding Source: UniProtKB
- positive regulation of protein kinase activity Source: UniProtKB
- positive regulation of vasculogenesis Source: UniProtKB
- Rap protein signal transduction Source: UniProtKB
- regulation of cell junction assembly Source: UniProtKB
- ventricular system development Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, GTPase activation, Guanine-nucleotide releasing factor |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
Reactomei | R-BTA-5673001, RAF/MAP kinase cascade |
Names & Taxonomyi
Protein namesi | Recommended name: Rap guanine nucleotide exchange factor 2Alternative name(s): Cyclic nucleotide ras GEF Short name: CNrasGEF Neural RAP guanine nucleotide exchange protein Short name: nRap GEP PDZ domain-containing guanine nucleotide exchange factor 1 Short name: PDZ-GEF1 RA-GEF-1 Ras/Rap1-associating GEF-1 |
Gene namesi | Name:RAPGEF2 Synonyms:NRAPGEP, PDZGEF1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:33723, RAPGEF2 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Endosome
- Late endosome By similarity
Other locations
- Cytoplasm By similarity
- perinuclear region By similarity
- Cell junction By similarity
Note: Associated with the synaptic plasma membrane. Localized diffusely in the cytoplasm before neuronal growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with the high affinity nerve growth factor receptor NTRK1 at late endosomes. Translocated to the perinuclear region in a RAP1A-dependent manner. Translocated to the cell membrane. Colocalized with CTNNB1 at cell-cell contacts (By similarity).By similarity
Endosome
- late endosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cell-cell junction Source: UniProtKB
- cytoplasm Source: UniProtKB
- neuron projection Source: UniProtKB
- neuronal cell body Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- protein-containing complex Source: UniProtKB
- synapse Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423853 | 1 – 1486 | Rap guanine nucleotide exchange factor 2Add BLAST | 1486 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 501 | PhosphoserineBy similarity | 1 | |
Modified residuei | 644 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 806 | PhosphoserineBy similarity | 1 | |
Modified residuei | 930 | PhosphoserineBy similarity | 1 | |
Modified residuei | 933 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1022 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1077 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1086 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1092 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1113 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1117 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1156 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1173 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | F1MSG6 |
PRIDEi | F1MSG6 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000012450, Expressed in prefrontal cortex and 18 other tissues |
ExpressionAtlasi | F1MSG6, baseline and differential |
Interactioni
Subunit structurei
Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a neuronal growth factor (NGF)-dependent manner.
Interacts (via C-terminal domain) with NEDD4 (via WW domains); this interaction leads to ubiquitination and degradation via the proteasome pathway in a cAMP-independent manner.
Interacts with MAGI1 (via PDZ domain).
Interacts with ADRB1 (via C-terminal PDZ motif); the interaction is direct.
Interacts (via Ras-associating domain) with RAP1A (via GTP-bound active form).
Interacts weakly with HRAS (via GDP- and GTP-bound forms).
Interacts (via C-terminal domain) with MAGI2 (via PDZ and WW domains).
Interacts with CDH1 and TJP1 (By similarity).
Interacts with CTNNB1.
By similarity1 PublicationBinary interactionsi
F1MSG6
With | #Exp. | IntAct |
---|---|---|
Ctnnb1 [Q02248] from Mus musculus. | 2 | EBI-6927068,EBI-397872 |
GO - Molecular functioni
- beta-1 adrenergic receptor binding Source: UniProtKB
- PDZ domain binding Source: UniProtKB
- Rap guanyl-nucleotide exchange factor activity Source: UniProtKB
- Ras guanyl-nucleotide exchange factor activity Source: UniProtKB
- WW domain binding Source: UniProtKB
Protein-protein interaction databases
IntActi | F1MSG6, 2 interactors |
STRINGi | 9913.ENSBTAP00000029739 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 267 – 380 | N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST | 114 | |
Domaini | 385 – 468 | PDZPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 606 – 692 | Ras-associatingPROSITE-ProRule annotationAdd BLAST | 87 | |
Domaini | 717 – 944 | Ras-GEFPROSITE-ProRule annotationAdd BLAST | 228 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 87 – 90 | Poly-Asp | 4 | |
Compositional biasi | 1105 – 1253 | Ser-richAdd BLAST | 149 | |
Compositional biasi | 1396 – 1471 | Pro-richAdd BLAST | 76 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3542, Eukaryota |
GeneTreei | ENSGT00940000156418 |
HOGENOMi | CLU_002782_0_1_1 |
InParanoidi | F1MSG6 |
OMAi | FPEGHAH |
OrthoDBi | 31139at2759 |
TreeFami | TF313184 |
Family and domain databases
CDDi | cd00038, CAP_ED, 1 hit cd00155, RasGEF, 1 hit cd06224, REM, 1 hit |
Gene3Di | 2.30.42.10, 1 hit 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR001478, PDZ IPR036034, PDZ_sf IPR000159, RA_dom IPR030739, RapGEF2 IPR008937, Ras-like_GEF IPR000651, Ras-like_Gua-exchang_fac_N IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR014710, RmlC-like_jellyroll IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR23113, PTHR23113, 1 hit PTHR23113:SF217, PTHR23113:SF217, 1 hit |
Pfami | View protein in Pfam PF00595, PDZ, 1 hit PF00788, RA, 1 hit PF00617, RasGEF, 1 hit PF00618, RasGEF_N, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 1 hit SM00228, PDZ, 1 hit SM00314, RA, 1 hit SM00147, RasGEF, 1 hit SM00229, RasGEFN, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit SSF50156, SSF50156, 1 hit SSF51206, SSF51206, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 1 hit PS50106, PDZ, 1 hit PS50200, RA, 1 hit PS50009, RASGEF_CAT, 1 hit PS50212, RASGEF_NTER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKPLAIPANH GVMGQQEKHS LPADFTKLHL TDSLHPQVTH VSSSHSGCSI
60 70 80 90 100
TSDSGSSSLS DIYQATESEA GDMDLSGLPE TAVDSEDDDD EEDIERASDP
110 120 130 140 150
LMSRDIVRDC LEKDPIDRTD DDIEQLLEFM HQLPAFANMT MSVRRELCAV
160 170 180 190 200
MVFAVVERAG TIVLNDGEEL DSWSVILNGS VEVTYPDGKA EILCMGNSFG
210 220 230 240 250
VSPTMDKEYM KGVMRTKVDD CQFVCIAQQD YCRILNQVEK NMQKVEEEGE
260 270 280 290 300
IVMVKEHREL DRTGTRKGHI VIKGTSERLT MHLVEEHSVV DPTFIEDFLL
310 320 330 340 350
TYRTFLCSPM EVGKKLLEWF NDPSLRDKVT RVVLLWVNNH FNDFEGDPAM
360 370 380 390 400
TRFLEEFENN LEREKMGGHL RLLNIACAAK AKRRLMTLTK PAREAPLPFI
410 420 430 440 450
LLGGSEKGFG IFVDSVDSAS KATEAGLKRG DQILEVNGQN FENIQLSKAM
460 470 480 490 500
EILRNNTHLS ITVKTNLFVF KELLTRLSEE KRNGAPHLPK IGDIKKASRY
510 520 530 540 550
SIPDLAVDVE QVIGLEKVNK KSKANTVGGR NKLKKILDKT RISILPQKPY
560 570 580 590 600
NDIGIGQSQD DSIVGLRQTK HIPTALPVSG TLSSSNPDLL QSHHRILDFS
610 620 630 640 650
ATPDLPDQVL RVFKADQQSR YIMISKDTTA KEVVVQAIRE FAVTATPDQY
660 670 680 690 700
SLCEVSVTPE GVIKQRRLPD QLSKLADRIQ LSGRYYLKNN METETLCSDE
710 720 730 740 750
DAQELLRESQ ISLLQLSTVE VATQLSMRNF ELFRNIEPTE YIDDLFKLKS
760 770 780 790 800
KTSCANLKTF EEVINQETFW VASEILRETN QLKRMKIIKH FIKIALHCRE
810 820 830 840 850
CKNFNSMFAI ISGLNLAPVA RLRTTWEKLP NKYEKLFQDL QDLFDPSRNM
860 870 880 890 900
AKYRNVLNSQ NLQPPIIPLF PVIKKDLTFL HEGNDSKVDG LVNFEKLRMI
910 920 930 940 950
AKEIRHVGRM ASVNMDPALM FRTRKKKWRS LGSLSQGSTN ATVLDVAQTG
960 970 980 990 1000
GHKKRVRRSS FLNAKKLYED AQMARKVKQY LSNLELEMDE ESLQTLSLQC
1010 1020 1030 1040 1050
EPATNTLPKN PTDKKPVKSE TSPVAPRAGL QPKAQPQPQP PQPPHKLNQG
1060 1070 1080 1090 1100
LQVPAVSLYP SRKKVPVKDL PPFGINSPQA LKKILSLSEE GSLERHRRPA
1110 1120 1130 1140 1150
EDTISNTSSQ LSSPPTSPQS SPRKGYTLAP SGTVDNFSDS GHSEISSRSS
1160 1170 1180 1190 1200
IVSNSSFDSL PVSLPDERRQ RPSVSIVETS LASGRLERRP AVEPDQYSLG
1210 1220 1230 1240 1250
SCAPLSESRG LYAAATVISS PSTEELSQDQ GDRASLDAAD SGRGSWTSCS
1260 1270 1280 1290 1300
SGSHDNIQTI QHQRSWETLP FGHTHFDYPG DAAGLWASSS HMDQIMFPDH
1310 1320 1330 1340 1350
SAKYSRQSQS RESLDQAQSR ASWASSTGYW GEDSEGDTGT IKRRGGKDVS
1360 1370 1380 1390 1400
LDADSSSMTA VTAEEAKPAA MAAHIAVTPS AAKGLIARKE GRYREPPPTP
1410 1420 1430 1440 1450
PGYVGIPITD FPEAHPHPAR KPPDYTVALQ RSRMLARPAE PPAPGSARPA
1460 1470 1480
PRPQWHRPGD GDPRAGPCAP PGLTAEEDED EQVSAV
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1LLB4 | A0A3Q1LLB4_BOVIN | Cyclic nucleotide ras GEF | RAPGEF2 | 1,648 | Annotation score: | ||
A0A3Q1LUK5 | A0A3Q1LUK5_BOVIN | Cyclic nucleotide ras GEF | RAPGEF2 | 1,484 | Annotation score: | ||
A0A3Q1LWT3 | A0A3Q1LWT3_BOVIN | Cyclic nucleotide ras GEF | RAPGEF2 | 1,640 | Annotation score: | ||
A0A3Q1M3K0 | A0A3Q1M3K0_BOVIN | Cyclic nucleotide ras GEF | RAPGEF2 | 1,459 | Annotation score: | ||
A0A3Q1N0F9 | A0A3Q1N0F9_BOVIN | Cyclic nucleotide ras GEF | RAPGEF2 | 1,642 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02044847 Genomic DNA No translation available. |
RefSeqi | NP_001178255.1, NM_001191326.1 |
Genome annotation databases
Ensembli | ENSBTAT00000029742; ENSBTAP00000029739; ENSBTAG00000012450 |
GeneIDi | 100139484 |
KEGGi | bta:100139484 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DAAA02044847 Genomic DNA No translation available. |
RefSeqi | NP_001178255.1, NM_001191326.1 |
3D structure databases
SMRi | F1MSG6 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | F1MSG6, 2 interactors |
STRINGi | 9913.ENSBTAP00000029739 |
Proteomic databases
PaxDbi | F1MSG6 |
PRIDEi | F1MSG6 |
Genome annotation databases
Ensembli | ENSBTAT00000029742; ENSBTAP00000029739; ENSBTAG00000012450 |
GeneIDi | 100139484 |
KEGGi | bta:100139484 |
Organism-specific databases
CTDi | 9693 |
VGNCi | VGNC:33723, RAPGEF2 |
Phylogenomic databases
eggNOGi | KOG3542, Eukaryota |
GeneTreei | ENSGT00940000156418 |
HOGENOMi | CLU_002782_0_1_1 |
InParanoidi | F1MSG6 |
OMAi | FPEGHAH |
OrthoDBi | 31139at2759 |
TreeFami | TF313184 |
Enzyme and pathway databases
Reactomei | R-BTA-5673001, RAF/MAP kinase cascade |
Gene expression databases
Bgeei | ENSBTAG00000012450, Expressed in prefrontal cortex and 18 other tissues |
ExpressionAtlasi | F1MSG6, baseline and differential |
Family and domain databases
CDDi | cd00038, CAP_ED, 1 hit cd00155, RasGEF, 1 hit cd06224, REM, 1 hit |
Gene3Di | 2.30.42.10, 1 hit 2.60.120.10, 1 hit |
InterProi | View protein in InterPro IPR018490, cNMP-bd-like IPR000595, cNMP-bd_dom IPR001478, PDZ IPR036034, PDZ_sf IPR000159, RA_dom IPR030739, RapGEF2 IPR008937, Ras-like_GEF IPR000651, Ras-like_Gua-exchang_fac_N IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR014710, RmlC-like_jellyroll IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR23113, PTHR23113, 1 hit PTHR23113:SF217, PTHR23113:SF217, 1 hit |
Pfami | View protein in Pfam PF00595, PDZ, 1 hit PF00788, RA, 1 hit PF00617, RasGEF, 1 hit PF00618, RasGEF_N, 1 hit |
SMARTi | View protein in SMART SM00100, cNMP, 1 hit SM00228, PDZ, 1 hit SM00314, RA, 1 hit SM00147, RasGEF, 1 hit SM00229, RasGEFN, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit SSF50156, SSF50156, 1 hit SSF51206, SSF51206, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50042, CNMP_BINDING_3, 1 hit PS50106, PDZ, 1 hit PS50200, RA, 1 hit PS50009, RASGEF_CAT, 1 hit PS50212, RASGEF_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPGF2_BOVIN | |
Accessioni | F1MSG6Primary (citable) accession number: F1MSG6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | November 16, 2011 | |
Last modified: | December 2, 2020 | |
This is version 67 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families