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Entry version 66 (16 Oct 2019)
Sequence version 2 (10 Apr 2019)
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Protein

DNA topoisomerase I

Gene

TOP1

Organism
Bos taurus (Bovine)
Status
Unreviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation EC:5.6.2.1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-bindingUniRule annotation, Isomerase, TopoisomeraseUniRule annotation

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-BTA-4615885 SUMOylation of DNA replication proteins

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA topoisomerase IUniRule annotation (EC:5.6.2.1UniRule annotation)
Alternative name(s):
DNA topoisomerase 1UniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TOP1Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBos taurus (Bovine)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9913 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000009136 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 13

Organism-specific databases

Vertebrate Gene Nomenclature Database

More...
VGNCi
VGNC:49136 TOP1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
F1MN93

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSBTAG00000007960 Expressed in 10 organ(s), highest expression level in spleen

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
9913.ENSBTAP00000010466

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini352 – 729TOPEUcInterPro annotationAdd BLAST378

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 191DisorderedSequence analysisAdd BLAST191

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili302 – 329Sequence analysisAdd BLAST28
Coiled coili636 – 670Sequence analysisAdd BLAST35
Coiled coili676 – 703Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi24 – 191PolyampholyteSequence analysisAdd BLAST168

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the type IB topoisomerase family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0981 Eukaryota
COG3569 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000155006

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
F1MN93

Database of Orthologous Groups

More...
OrthoDBi
303947at2759

TreeFam database of animal gene trees

More...
TreeFami
TF105281

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00659 Topo_IB_C, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 1 hit
3.90.15.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR018521 TopoI_AS
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00416 EUTPISMRASEI

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00435 TOPEUc, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56349 SSF56349, 1 hit
SSF56741 SSF56741, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

F1MN93-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
DSSLMVADSH KHKDKHKDRE HRHKEHKKDK EKDREKSKHS NSEHKDSEKK
60 70 80 90 100
HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKIKASGDA KIKKEKENGF
110 120 130 140 150
SSPPRIKDEP EDDGYFAPPK EDIKPLKRPR DEDDADYKPK KIKTEDIKKE
160 170 180 190 200
KKRKLEEEED GKLKKPKNKD KDKDKKVPEP DSKKKKPKKE EEQKWKWWEE
210 220 230 240 250
ERYPEGIKWK FLEHKGPVFA PPYEPLPENV KFYYDGKVMK LSPKAEEVAT
260 270 280 290 300
FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN EEKNIITNLS KCDFTQMSQY
310 320 330 340 350
FKAQTEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP
360 370 380 390 400
GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN
410 420 430 440 450
KVTWLVSWTE NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR
460 470 480 490 500
NQYREDWKSK EMKVRQRAVA LYFIDKLALR AGNEKEEGET ADTVGCCSLR
510 520 530 540 550
VEHINLHPEL DGQEYVVEFD FLGKDSIRYY NKVPVEKRVF KNLQLFMENK
560 570 580 590 600
QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL QQQLKELTAP
610 620 630 640 650
DENIPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKEQL
660 670 680 690 700
ADARRDLKSA KADAKVLKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR
710 720 730 740 750
EENKQIALGT SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM

ADEDYEF
Length:757
Mass (Da):89,688
Last modified:April 10, 2019 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4E516EE22A984B7F
GO

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSBTAT00000010466; ENSBTAP00000010466; ENSBTAG00000007960

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000010466

Proteomic databases

PaxDbiF1MN93

Genome annotation databases

EnsembliENSBTAT00000010466; ENSBTAP00000010466; ENSBTAG00000007960

Organism-specific databases

VGNCiVGNC:49136 TOP1

Phylogenomic databases

eggNOGiKOG0981 Eukaryota
COG3569 LUCA
GeneTreeiENSGT00940000155006
InParanoidiF1MN93
OrthoDBi303947at2759
TreeFamiTF105281

Enzyme and pathway databases

ReactomeiR-BTA-4615885 SUMOylation of DNA replication proteins

Gene expression databases

BgeeiENSBTAG00000007960 Expressed in 10 organ(s), highest expression level in spleen

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 1 hit
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR018521 TopoI_AS
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk
PfamiView protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SMARTiView protein in SMART
SM00435 TOPEUc, 1 hit
SUPFAMiSSF56349 SSF56349, 1 hit
SSF56741 SSF56741, 1 hit
PROSITEiView protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiF1MN93_BOVIN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: F1MN93
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: April 10, 2019
Last modified: October 16, 2019
This is version 66 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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