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Entry version 70 (02 Jun 2021)
Sequence version 2 (03 Apr 2013)
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Protein

Neutrophil cytosolic factor 1

Gene

Ncf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

By similarity

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-RNO-1222556, ROS and RNS production in phagocytes
R-RNO-1236973, Cross-presentation of particulate exogenous antigens (phagosomes)
R-RNO-3299685, Detoxification of Reactive Oxygen Species
R-RNO-4420097, VEGFA-VEGFR2 Pathway
R-RNO-5668599, RHO GTPases Activate NADPH Oxidases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Neutrophil cytosolic factor 1Imported
Short name:
NCF-1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Ncf1Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 12
  • UP000234681 Componentsi: Chromosome 12, Unassembled WGS sequence

Organism-specific databases

Rat genome database

More...
RGDi
61307, Ncf1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywords - Cellular componenti

Cytoplasm, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004339271 – 389Neutrophil cytosolic factor 1Add BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei304PhosphoserineBy similarity1
Modified residuei321PhosphoserineCombined sources1
Modified residuei329PhosphoserineBy similarity1
Modified residuei346PhosphoserineBy similarity1
Modified residuei349PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
F1M707

PRoteomics IDEntifications database

More...
PRIDEi
F1M707

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
F1M707

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSRNOG00000001480, Expressed in spleen and 21 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
F1M707, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
F1M707, RN

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4.

Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain).

Interacts with NCF4.

Interacts with CYBB.

Interacts (via the second SH3 domain) with CYBA.

Interacts with NOXA1.

Interacts with ADAM15.

Interacts with TRAF4.

Interacts with FASLG (By similarity).

Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity).

By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
10116.ENSRNOP00000002028

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
F1M707

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini4 – 125PXPROSITE-ProRule annotationAdd BLAST122
Domaini156 – 215SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini226 – 285SH3 2PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni292 – 389DisorderedSequence analysisAdd BLAST98

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state (By similarity).By similarity

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG4773, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000160014

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
F1M707

Identification of Orthologs from Complete Genome Data

More...
OMAi
WWFCQLK

TreeFam database of animal gene trees

More...
TreeFami
TF329347

Family and domain databases

Conserved Domains Database

More...
CDDi
cd06887, PX_p47phox, 1 hit
cd12021, SH3_p47phox_1, 1 hit
cd12022, SH3_p47phox_2, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1520.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR015039, NADPH_oxidase_p47Phox_C
IPR035756, NCF1_SH3_1
IPR035757, NCF1_SH3_2
IPR032136, NECFESHC
IPR001655, P47PHOX
IPR001683, PX_dom
IPR036871, PX_dom_sf
IPR034909, PX_p47phox
IPR036028, SH3-like_dom_sf
IPR001452, SH3_domain

The PANTHER Classification System

More...
PANTHERi
PTHR15706:SF6, PTHR15706:SF6, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF16621, NECFESHC, 1 hit
PF08944, p47_phox_C, 1 hit
PF00787, PX, 1 hit
PF00018, SH3_1, 2 hits

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00498, P47PHOX
PR00452, SH3DOMAIN

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00312, PX, 1 hit
SM00326, SH3, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50044, SSF50044, 2 hits
SSF64268, SSF64268, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50195, PX, 1 hit
PS50002, SH3, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

F1M707-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRKFTEIYEF
60 70 80 90 100
HKMLKEMFPI EAGEIHTENR VIPHLPAPRW YDGQRAAESR QGTLTEYFNS
110 120 130 140 150
LMGLPMKISR CPHLLNFFKV RPDDLKLPND SQVKKPETYL TAKDGKNNVA
160 170 180 190 200
DITGPIILQT YRAIADYEKG SKTEMTVATG DVVDVVEKSE SGWWFCQMKT
210 220 230 240 250
KRGWVPASYL EPLDSPDEAE DPDPNYAGEP YVTIKAYAAV EEDEVSLSEG
260 270 280 290 300
EAIEVIHKLL DGWWVVRKGD ITGYFPSMYL QKAGEEITQA QRQIRSRGAP
310 320 330 340 350
PRRSTIRNAQ SIHQRSRKRL SQDTYRRNSV RFLQQRRRPA RPGPQSPDSK
360 370 380
DNPSTPRAKP QPAVPPRPSS DLILHRCTES TKRKLTSAV
Length:389
Mass (Da):44,712
Last modified:April 3, 2013 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBB71832F5434E80C
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A096MJP6A0A096MJP6_RAT
Neutrophil cytosolic factor 1
Ncf1
268Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AABR07035855 Genomic DNA No translation available.
CH473973 Genomic DNA Translation: EDM13444.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSRNOT00000002028; ENSRNOP00000002028; ENSRNOG00000001480

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR07035855 Genomic DNA No translation available.
CH473973 Genomic DNA Translation: EDM13444.1

3D structure databases

SMRiF1M707
ModBaseiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002028

PTM databases

iPTMnetiF1M707

Proteomic databases

PaxDbiF1M707
PRIDEiF1M707

Genome annotation databases

EnsembliENSRNOT00000002028; ENSRNOP00000002028; ENSRNOG00000001480

Organism-specific databases

RGDi61307, Ncf1

Phylogenomic databases

eggNOGiKOG4773, Eukaryota
GeneTreeiENSGT00940000160014
InParanoidiF1M707
OMAiWWFCQLK
TreeFamiTF329347

Enzyme and pathway databases

ReactomeiR-RNO-1222556, ROS and RNS production in phagocytes
R-RNO-1236973, Cross-presentation of particulate exogenous antigens (phagosomes)
R-RNO-3299685, Detoxification of Reactive Oxygen Species
R-RNO-4420097, VEGFA-VEGFR2 Pathway
R-RNO-5668599, RHO GTPases Activate NADPH Oxidases

Miscellaneous databases

Protein Ontology

More...
PROi
PR:F1M707

Gene expression databases

BgeeiENSRNOG00000001480, Expressed in spleen and 21 other tissues
ExpressionAtlasiF1M707, baseline and differential
GenevisibleiF1M707, RN

Family and domain databases

CDDicd06887, PX_p47phox, 1 hit
cd12021, SH3_p47phox_1, 1 hit
cd12022, SH3_p47phox_2, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR015039, NADPH_oxidase_p47Phox_C
IPR035756, NCF1_SH3_1
IPR035757, NCF1_SH3_2
IPR032136, NECFESHC
IPR001655, P47PHOX
IPR001683, PX_dom
IPR036871, PX_dom_sf
IPR034909, PX_p47phox
IPR036028, SH3-like_dom_sf
IPR001452, SH3_domain
PANTHERiPTHR15706:SF6, PTHR15706:SF6, 1 hit
PfamiView protein in Pfam
PF16621, NECFESHC, 1 hit
PF08944, p47_phox_C, 1 hit
PF00787, PX, 1 hit
PF00018, SH3_1, 2 hits
PRINTSiPR00498, P47PHOX
PR00452, SH3DOMAIN
SMARTiView protein in SMART
SM00312, PX, 1 hit
SM00326, SH3, 2 hits
SUPFAMiSSF50044, SSF50044, 2 hits
SSF64268, SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195, PX, 1 hit
PS50002, SH3, 2 hits

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNCF1_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: F1M707
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: April 3, 2013
Last modified: June 2, 2021
This is version 70 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome
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