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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Grosmannia clavigera (strain kw1407 / UAMH 11150) (Blue stain fungus) (Graphiocladiella clavigera)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotationSAAS annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei170S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei173UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, RNA-binding, Transferase, tRNA-bindingUniRule annotationSAAS annotation
Biological processtRNA processingUniRule annotationSAAS annotation
LigandS-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayi
UPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:CMQ_3539Imported
OrganismiGrosmannia clavigera (strain kw1407 / UAMH 11150) (Blue stain fungus) (Graphiocladiella clavigera)Imported
Taxonomic identifieri655863 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeOphiostomatalesOphiostomataceaeGrosmannia
Proteomesi
  • UP000007796 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 105S-adenosyl-L-methionine bindingUniRule annotation2
Regioni150 – 151S-adenosyl-L-methionine bindingUniRule annotation2
Regioni270 – 272S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotationSAAS annotation

Phylogenomic databases

InParanoidiF0X9H9
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

F0X9H9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKRQKRESY LLAQQRPDGA VPELPQKKFY RQRAHANPFS DHQLVYPASP
60 70 80 90 100
DAMDWASLYP DFFPAAATEE QQQQVEVADI GCGFGGLLVG LSPVMPETLI
110 120 130 140 150
LGMEIRDHVT EFVQNRIHAL RAQGREAAGA DEETTTGPSA FANIACVRAN
160 170 180 190 200
TMKFLPNFFR KAQLRKIFLC FPDPHFKARK HKARIVSPTL AAEYAYVLRP
210 220 230 240 250
GCGRVYTITD VEDLHRWMVA HFAAHPAFAR LSPEEEAAGA EEEDGQTGKN
260 270 280 290 300
GKDGKDDTSD PCIRVMRTAT EEGKKVARNK GQKFVAVFRR LEDPPWPTDT

L
Length:301
Mass (Da):33,698
Last modified:May 3, 2011 - v1
Checksum:iCF01184AD11A1B1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL629735 Genomic DNA Translation: EFX05470.1
RefSeqiXP_014174952.1, XM_014319477.1

Genome annotation databases

EnsemblFungiiEFX05470; EFX05470; CMQ_3539
GeneIDi25976650

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GL629735 Genomic DNA Translation: EFX05470.1
RefSeqiXP_014174952.1, XM_014319477.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFX05470; EFX05470; CMQ_3539
GeneIDi25976650

Phylogenomic databases

InParanoidiF0X9H9
OrthoDBiEOG092C3FCG

Enzyme and pathway databases

UniPathwayi
UPA00989

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiF0X9H9_GROCL
AccessioniPrimary (citable) accession number: F0X9H9
Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: May 23, 2018
This is version 37 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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