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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Chlamydophila psittaci (strain ATCC VR-125 / 6BC) (Chlamydia psittaci)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of predominantly four 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Thus generates the genus-specific LPS epitope of Chlamydia, composed of the trisaccharide alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo.3 Publications

Catalytic activityi

Lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications
Alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->8)-(alpha-Kdo-(2->4))-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.2 Publications

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei70Proton acceptorBy similarity1
Sitei141Transition state stabilizerBy similarity1
Sitei219Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15506
BRENDAi2.4.99.12 1312
2.4.99.13 1312
2.4.99.14 1312
UniPathwayiUPA00958

Protein family/group databases

CAZyiGT30 Glycosyltransferase Family 30

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.122 Publications, EC:2.4.99.132 Publications, EC:2.4.99.142 Publications, EC:2.4.99.152 Publications)
Short name:
Kdo transferase
Alternative name(s):
Kdo(2)-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Kdo(3)-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Multifunctional Kdo transferase
Gene namesi
Name:waaA
Synonyms:gseA
Ordered Locus Names:CPSIT_0652, G5O_0645
OrganismiChlamydophila psittaci (strain ATCC VR-125 / 6BC) (Chlamydia psittaci)
Taxonomic identifieri331636 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31Helical; Signal-anchorSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004191731 – 4333-deoxy-D-manno-octulosonic acid transferaseAdd BLAST433

Structurei

3D structure databases

SMRiF0T4D1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 278CMP-Kdo bindingBy similarity2
Regioni317 – 319CMP-Kdo bindingBy similarity3
Regioni344 – 347CMP-Kdo bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D8A Bacteria
COG1519 LUCA
KOiK02527
OMAiFIKYEFW

Family and domain databases

Gene3Di3.40.50.11720, 1 hit
InterProiView protein in InterPro
IPR007507 Glycos_transf_N
IPR038107 Glycos_transf_N_sf
PfamiView protein in Pfam
PF04413 Glycos_transf_N, 1 hit

Sequencei

Sequence statusi: Complete.

F0T4D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKGRRTKLH TFLYDCFLIF AFMVGLPRIL YKRFVHGKYT KSLGIRFGFK
60 70 80 90 100
KPEVPGTGPV AWFHGASVGE TALLLPLLKR FMKEYPEWRC VVTSCTESGH
110 120 130 140 150
ENAHRLFGPL GVTTFILPLD LSIIIKPVVR AISPSLLVFS EGDCWLNFIE
160 170 180 190 200
EAKRLGATAV IINGKLSANS CKRFTILKRF GRNYFSPVDG FLLQDEQHKA
210 220 230 240 250
RFLQLGVDKE KIQVTGNIKT YTETLSENNQ RDYWREKLQL AQDTELLVLG
260 270 280 290 300
SVHPKDVEVW LPVVRELRRN LKVLWVPRHI ERSKELEALL SKENISYGLW
310 320 330 340 350
SKEATFAQHD AIIVDAIGWL KQLYSAADLA FVGGTFDDRI GGHNLLEPLQ
360 370 380 390 400
CGVPLIFGPH IQSQSDLAER LLSMGAGCCL DKTNIVKVIT FLLDHPEERA
410 420 430
AYIQKGAMFL HEEKVAFDRT WESFKRYIPC VKI
Length:433
Mass (Da):49,322
Last modified:May 3, 2011 - v1
Checksum:i6DFD04C3A2885F0B
GO

Sequence cautioni

The sequence CAA49233 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69476 Genomic DNA Translation: CAA49233.1 Different initiation.
CP002549 Genomic DNA Translation: ADZ18468.1
CP002586 Genomic DNA Translation: AEB55626.1

Genome annotation databases

EnsemblBacteriaiADZ18468; ADZ18468; CPSIT_0652
AEB55626; AEB55626; G5O_0645
KEGGichb:G5O_0645
chp:CPSIT_0652
PATRICifig|331636.3.peg.626

Similar proteinsi

Entry informationi

Entry nameiKDTA_CHLP6
AccessioniPrimary (citable) accession number: F0T4D1
Secondary accession number(s): Q06380
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: May 3, 2011
Last modified: June 20, 2018
This is version 38 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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