UniProtKB - E9QPD7 (E9QPD7_MOUSE)
Protein
Pyruvate carboxylase
Gene
Pcx
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.UniRule annotation
Catalytic activityi
- ATP + hydrogencarbonate + pyruvate = ADP + H+ + oxaloacetate + phosphateUniRule annotationARBA annotationEC:6.4.1.1UniRule annotationARBA annotation
Cofactori
biotinUniRule annotationARBA annotation
: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.ARBA annotationView all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 153 | ATPUniRule annotation | 1 | |
Binding sitei | 237 | ATPUniRule annotation | 1 | |
Binding sitei | 272 | ATPUniRule annotation | 1 | |
Active sitei | 329 | UniRule annotation | 1 | |
Metal bindingi | 573 | Divalent metal cationUniRule annotation | 1 | |
Binding sitei | 645 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 742 | Divalent metal cation; via carbamate groupUniRule annotation | 1 | |
Metal bindingi | 772 | Divalent metal cationUniRule annotation | 1 | |
Metal bindingi | 774 | Divalent metal cationUniRule annotation | 1 | |
Binding sitei | 909 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- biotin binding Source: InterPro
- identical protein binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- pyruvate carboxylase activity Source: UniProtKB-EC
GO - Biological processi
- gluconeogenesis Source: UniProtKB-UniPathway
- negative regulation of gene expression Source: Ensembl
- positive regulation by host of viral release from host cell Source: Ensembl
- pyruvate metabolic process Source: InterPro
- viral RNA genome packaging Source: Ensembl
Keywordsi
Molecular function | LigaseUniRule annotationARBA annotation |
Biological process | GluconeogenesisARBA annotation |
Ligand | ATP-bindingUniRule annotationARBA annotation, BiotinUniRule annotationARBA annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateARBA annotation |
Enzyme and pathway databases
UniPathwayi | UPA00138 |
Names & Taxonomyi
Protein namesi | Recommended name: Pyruvate carboxylaseUniRule annotationARBA annotation (EC:6.4.1.1UniRule annotationARBA annotation) |
Gene namesi | Name:PcxImported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97520, Pcx |
PTM / Processingi
Proteomic databases
jPOSTi | E9QPD7 |
MaxQBi | E9QPD7 |
PeptideAtlasi | E9QPD7 |
PRIDEi | E9QPD7 |
ProteomicsDBi | 355402 |
PTM databases
SwissPalmi | E9QPD7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024892, Expressed in mammary gland and 342 other tissues |
ExpressionAtlasi | E9QPD7, baseline and differential |
Genevisiblei | E9QPD7, MM |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 37 – 487 | Biotin carboxylationInterPro annotationAdd BLAST | 451 | |
Domaini | 157 – 354 | ATP-graspInterPro annotationAdd BLAST | 198 | |
Domaini | 564 – 833 | Pyruvate carboxyltransferaseInterPro annotationAdd BLAST | 270 | |
Domaini | 1110 – 1179 | Lipoyl-bindingInterPro annotationAdd BLAST | 70 |
Phylogenomic databases
GeneTreei | ENSGT00900000141164 |
OrthoDBi | 254436at2759 |
TreeFami | TF300535 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR003379, Carboxylase_cons_dom IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR000891, PYR_CT IPR005930, Pyruv_COase IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF00682, HMGL-like, 1 hit PF02436, PYC_OADA, 1 hit |
PIRSFi | PIRSF001594, Pyruv_carbox, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01235, pyruv_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS50991, PYR_CT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
E9QPD7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMLKFQTVRG GLRLLGVRRS SSAPVASPNV RRLEYKPIKK VMVANRGEIA
60 70 80 90 100
IRVFRACTEL GIRTVAVYSE QDTGQMHRQK ADEAYLIGRG LAPVQAYLHI
110 120 130 140 150
PDIIKVAKEN GVDAVHPGYG FLSERADFAQ ACQDAGVRFI GPSPEVVRKM
160 170 180 190 200
GDKVEARAIA IAAGVPVVPG TDSPISSLHE AHEFSNTYGF PIIFKAAYGG
210 220 230 240 250
GGRGMRVVHS YEELEENYTR AYSEALAAFG NGALFVEKFI EKPRHIEVQI
260 270 280 290 300
LGDQYGNILH LYERDCSIQR RHQKVVEIAP ATHLDPQLRS RLTSDSVKLA
310 320 330 340 350
KQVGYENAGT VEFLVDKHGK HYFIEVNSRL QVEHTVTEEI TDVDLVHAQI
360 370 380 390 400
HVSEGRSLPD LGLRQENIRI NGCAIQCRVT TEDPARSFQP DTGRIEVFRS
410 420 430 440 450
GEGMGIRLDN ASAFQGAVIS PHYDSLLVKV IAHGKDHPTA ATKMSRALAE
460 470 480 490 500
FRVRGVKTNI PFLQNVLNNQ QFLAGTVDTQ FIDENPELFQ LRPAQNRAQK
510 520 530 540 550
LLHYLGHVMV NGPTTPIPVN VSPSPVDPAV PVVPIGPPPA GFRDILLREG
560 570 580 590 600
PEGFARAVRN HQGLLLMDTT FRDAHQSLLA TRVRTHDLKK IAPYVAHNFN
610 620 630 640 650
KLFSMENWGG ATFDVAMRFL YECPWRRLQE LRELIPNIPF QMLLRGANAV
660 670 680 690 700
GYTNYPDNVV FKFCEVAKEN GMDVFRVFDS LNYLPNMLLG MEAAGSAGGV
710 720 730 740 750
VEAAISYTGD VADPSRTKYS LEYYMGLAEE LVRAGTHILC IKDMAGLLKP
760 770 780 790 800
AACTMLVSSL RDRFPDLPLH IHTHDTSGAG VAAMLACAQA GADVVDVAVD
810 820 830 840 850
SMSGMTSQPS MGALVACTKG TPLDTEVPLE RVFDYSEYWE GARGLYAAFD
860 870 880 890 900
CTATMKSGNS DVYENEIPGG QYTNLHFQAH SMGLGSKFKE VKKAYVEANQ
910 920 930 940 950
MLGDLIKVTP SSKIVGDLAQ FMVQNGLSRA EAEAQAEELS FPRSVVEFLQ
960 970 980 990 1000
GYIGIPHGGF PEPFRSKVLK DLPRIEGRPG ASLPPLNLKE LEKDLIDRHG
1010 1020 1030 1040 1050
EEVTPEDVLS AAMYPDVFAQ FKDFTATFGP LDSLNTRLFL QGPKIAEEFE
1060 1070 1080 1090 1100
VELERGKTLH IKALAVSDLN RAGQRQVFFE LNGQLRSILV KDTQAMKEMH
1110 1120 1130 1140 1150
FHPKALKDVK GQIGAPMPGK VIDIKVAAGD KVAKGQPLCV LSAMKMETVV
1160 1170
TSPMEGTIRK VHVTKDMTLE GDDLILEIE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ05920 | PYC_MOUSE | Pyruvate carboxylase, mitochondrial | Pc Pcx | 1,178 | Annotation score: | ||
G5E8R3 | G5E8R3_MOUSE | Pyruvate carboxylase | Pcx | 1,178 | Annotation score: | ||
A0A286YCC5 | A0A286YCC5_MOUSE | Pyruvate carboxylase | Pcx | 116 | Annotation score: | ||
A0A494B912 | A0A494B912_MOUSE | Pyruvate carboxylase | Pcx | 120 | Annotation score: | ||
A0A494B951 | A0A494B951_MOUSE | Pyruvate carboxylase | Pcx | 97 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC124347 Genomic DNA No translation available. AC128739 Genomic DNA No translation available. |
RefSeqi | NP_001156418.1, NM_001162946.1 |
Genome annotation databases
Ensembli | ENSMUST00000068004; ENSMUSP00000063825; ENSMUSG00000024892 |
GeneIDi | 18563 |
KEGGi | mmu:18563 |
UCSCi | uc008gaf.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC124347 Genomic DNA No translation available. AC128739 Genomic DNA No translation available. |
RefSeqi | NP_001156418.1, NM_001162946.1 |
3D structure databases
SMRi | E9QPD7 |
ModBasei | Search... |
PTM databases
SwissPalmi | E9QPD7 |
Proteomic databases
jPOSTi | E9QPD7 |
MaxQBi | E9QPD7 |
PeptideAtlasi | E9QPD7 |
PRIDEi | E9QPD7 |
ProteomicsDBi | 355402 |
Protocols and materials databases
Antibodypediai | 30264, 354 antibodies |
DNASUi | 18563 |
Genome annotation databases
Ensembli | ENSMUST00000068004; ENSMUSP00000063825; ENSMUSG00000024892 |
GeneIDi | 18563 |
KEGGi | mmu:18563 |
UCSCi | uc008gaf.2, mouse |
Organism-specific databases
CTDi | 18563 |
MGIi | MGI:97520, Pcx |
Phylogenomic databases
GeneTreei | ENSGT00900000141164 |
OrthoDBi | 254436at2759 |
TreeFami | TF300535 |
Enzyme and pathway databases
UniPathwayi | UPA00138 |
Miscellaneous databases
BioGRID-ORCSi | 18563, 0 hits in 52 CRISPR screens |
ChiTaRSi | Pcx, mouse |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024892, Expressed in mammary gland and 342 other tissues |
ExpressionAtlasi | E9QPD7, baseline and differential |
Genevisiblei | E9QPD7, MM |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR011761, ATP-grasp IPR005481, BC-like_N IPR001882, Biotin_BS IPR011764, Biotin_carboxylation_dom IPR005482, Biotin_COase_C IPR000089, Biotin_lipoyl IPR003379, Carboxylase_cons_dom IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR016185, PreATP-grasp_dom_sf IPR000891, PYR_CT IPR005930, Pyruv_COase IPR011054, Rudment_hybrid_motif IPR011053, Single_hybrid_motif |
Pfami | View protein in Pfam PF02785, Biotin_carb_C, 1 hit PF00289, Biotin_carb_N, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02786, CPSase_L_D2, 1 hit PF00682, HMGL-like, 1 hit PF02436, PYC_OADA, 1 hit |
PIRSFi | PIRSF001594, Pyruv_carbox, 1 hit |
SMARTi | View protein in SMART SM00878, Biotin_carb_C, 1 hit |
SUPFAMi | SSF51230, SSF51230, 1 hit SSF51246, SSF51246, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01235, pyruv_carbox, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS50979, BC, 1 hit PS00188, BIOTIN, 1 hit PS50968, BIOTINYL_LIPOYL, 1 hit PS50991, PYR_CT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | E9QPD7_MOUSE | |
Accessioni | E9QPD7Primary (citable) accession number: E9QPD7 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | April 5, 2011 |
Last sequence update: | April 5, 2011 | |
Last modified: | April 7, 2021 | |
This is version 83 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |