We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - E9QI36 (HARS1_DANRE)
Protein
Histidine--tRNA ligase
Gene
hars
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Catalyzes the aminoacylation of histidyl-tRNA.
By similarityMiscellaneous
Danio rerio genome encodes a single hars gene which undergoes alternative splicing to produce both a cytoplasmic and mitochondrial enzyme, while humans have two separate genes.1 Publication
Catalytic activityi
- EC:6.1.1.21By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 157 | L-histidineBy similarity | 1 | |
Binding sitei | 173 | L-histidineBy similarity | 1 | |
Binding sitei | 177 | L-histidineBy similarity | 1 | |
Binding sitei | 326 | L-histidineBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histidine-tRNA ligase activity Source: UniProtKB-EC
GO - Biological processi
- histidyl-tRNA aminoacylation Source: InterPro
- regulation of vasculature development Source: ZFIN
- sprouting angiogenesis Source: ZFIN
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:harsImported |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-040912-152, hars |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
ChainiPRO_0000448899 | 1 – 513 | Histidine--tRNA ligaseAdd BLAST | 513 | ||
Isoform 2 (identifier: E9QI36-2) | |||||
Transit peptidei | 1 – 24 | MitochondrionSequence analysisAdd BLAST | 24 |
Proteomic databases
PaxDbi | F1Q5D5 |
PeptideAtlasi | E9QI36 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000003693, Expressed in somite and 34 other tissues |
ExpressionAtlasi | E9QI36, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 59 | WHEP-TRSPROSITE-ProRule annotationAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 132 | L-histidine bindingBy similarity | 3 | |
Regioni | 330 – 331 | L-histidine bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG1936, Eukaryota |
GeneTreei | ENSGT00390000005922 |
HOGENOMi | CLU_025113_4_2_1 |
InParanoidi | F1Q5D5 |
OMAi | CDFDFIG |
OrthoDBi | 1065556at2759 |
TreeFami | TF300652 |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
Gene3Di | 3.30.930.10, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon IPR009068, S15_NS1_RNA-bd IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit PF00458, WHEP-TRS, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: E9QI36-1) [UniParc]FASTAAdd to basket
Also known as: Hars-0021 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADKAQLQEA IKTQGEVVRK LKSEKASKEQ IDEEVARLLQ LKAQLGGDEG
60 70 80 90 100
KHVFVLKTAK GTRDYNPKQM AIREKVFNII INCFKRHGAE TIDSPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKITNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QYDAMIPDAE CLKLVYEILS
210 220 230 240 250
ELDLGDFRIK VNDRRILDGM FAICGVPDEK FRTICSTVDK LDKLAWEEVK
260 270 280 290 300
KEMVNEKGLS EEVADRIRDY VSMQGGKDLA ERLLQDPKLS QSKQACAGIT
310 320 330 340 350
DMKLLFSYLE LFQITDKVVF DLSLARGLDY YTGVIYEAIL TQANPAPAST
360 370 380 390 400
PAEQNGAEDA GVSVGSVAGG GRYDGLVGMF DPKGRKVPCV GVSIGIERVF
410 420 430 440 450
SIMEQKAELS SEKVRTTETQ VLVASAQKNL LEERLKLTAE LWNAGIKAEV
460 470 480 490 500
LYKKNPKLLS QLQHCEDTGI PLVAILGEQE LKDGVVKLRN VASREEVDVP
510
RAELVDEVKK RTS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060465 | 3 – 29 | DKAQL…KASKE → ALGLVSMRLCAGLMGRRSAV RLHSLRVCSGMTIS in isoform 2. Add BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX890575 Genomic DNA No translation available. |
RefSeqi | NP_001289185.1, NM_001302256.1 [E9QI36-2] NP_001289191.1, NM_001302262.1 [E9QI36-1] |
Genome annotation databases
Ensembli | ENSDART00000141471; ENSDARP00000123163; ENSDARG00000003693 [E9QI36-2] ENSDART00000142158; ENSDARP00000113894; ENSDARG00000003693 [E9QI36-1] |
GeneIDi | 447847 |
KEGGi | dre:447847 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX890575 Genomic DNA No translation available. |
RefSeqi | NP_001289185.1, NM_001302256.1 [E9QI36-2] NP_001289191.1, NM_001302262.1 [E9QI36-1] |
3D structure databases
SMRi | E9QI36 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000123163 |
Proteomic databases
PaxDbi | F1Q5D5 |
PeptideAtlasi | E9QI36 |
Genome annotation databases
Ensembli | ENSDART00000141471; ENSDARP00000123163; ENSDARG00000003693 [E9QI36-2] ENSDART00000142158; ENSDARP00000113894; ENSDARG00000003693 [E9QI36-1] |
GeneIDi | 447847 |
KEGGi | dre:447847 |
Organism-specific databases
CTDi | 15115 |
ZFINi | ZDB-GENE-040912-152, hars |
Phylogenomic databases
eggNOGi | KOG1936, Eukaryota |
GeneTreei | ENSGT00390000005922 |
HOGENOMi | CLU_025113_4_2_1 |
InParanoidi | F1Q5D5 |
OMAi | CDFDFIG |
OrthoDBi | 1065556at2759 |
TreeFami | TF300652 |
Miscellaneous databases
PROi | PR:E9QI36 |
Gene expression databases
Bgeei | ENSDARG00000003693, Expressed in somite and 34 other tissues |
ExpressionAtlasi | E9QI36, baseline and differential |
Family and domain databases
CDDi | cd00773, HisRS-like_core, 1 hit cd00859, HisRS_anticodon, 1 hit |
Gene3Di | 3.30.930.10, 1 hit 3.40.50.800, 1 hit |
HAMAPi | MF_00127, His_tRNA_synth, 1 hit |
InterProi | View protein in InterPro IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR015807, His-tRNA-ligase IPR041715, HisRS-like_core IPR004516, HisRS/HisZ IPR033656, HisRS_anticodon IPR009068, S15_NS1_RNA-bd IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF13393, tRNA-synt_His, 1 hit PF00458, WHEP-TRS, 1 hit |
PIRSFi | PIRSF001549, His-tRNA_synth, 1 hit |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00442, hisS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HARS1_DANRE | |
Accessioni | E9QI36Primary (citable) accession number: E9QI36 Secondary accession number(s): F1Q5D5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2020 |
Last sequence update: | January 20, 2016 | |
Last modified: | February 23, 2022 | |
This is version 67 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families