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Entry version 66 (10 Feb 2021)
Sequence version 2 (20 Jan 2016)
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Protein

Histidine--tRNA ligase

Gene

hars

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the aminoacylation of histidyl-tRNA.By similarity

Miscellaneous

Danio rerio genome encodes a single hars gene which undergoes alternative splicing to produce both a cytoplasmic and mitochondrial enzyme, while humans have two separate genes.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei157L-histidineBy similarity1
Binding sitei173L-histidineBy similarity1
Binding sitei177L-histidineBy similarity1
Binding sitei326L-histidineBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Histidine--tRNA ligase (EC:6.1.1.21By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:harsImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7955 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesDanionidaeDanioninaeDanio
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000437 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 14

Organism-specific databases

Zebrafish Information Network genome database

More...
ZFINi
ZDB-GENE-040912-152, hars

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004488991 – 513Histidine--tRNA ligaseAdd BLAST513
Isoform 2 (identifier: E9QI36-2)
<p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 24MitochondrionSequence analysisAdd BLAST24

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
F1Q5D5

PeptideAtlas

More...
PeptideAtlasi
E9QI36

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSDARG00000003693, Expressed in somite and 34 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
E9QI36, baseline and differential

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
E9QI36

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini3 – 59WHEP-TRSPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni130 – 132L-histidine bindingBy similarity3
Regioni330 – 331L-histidine bindingBy similarity2

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1936, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000005922

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_025113_4_2_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
F1Q5D5

Identification of Orthologs from Complete Genome Data

More...
OMAi
CDFDFIG

Database of Orthologous Groups

More...
OrthoDBi
1065556at2759

TreeFam database of animal gene trees

More...
TreeFami
TF300652

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00773, HisRS-like_core, 1 hit
cd00859, HisRS_anticodon, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.800, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00127, His_tRNA_synth, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006195, aa-tRNA-synth_II
IPR004154, Anticodon-bd
IPR036621, Anticodon-bd_dom_sf
IPR015807, His-tRNA-ligase
IPR041715, HisRS-like_core
IPR004516, HisRS/HisZ
IPR033656, HisRS_anticodon
IPR009068, S15_NS1_RNA-bd
IPR000738, WHEP-TRS_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03129, HGTP_anticodon, 1 hit
PF13393, tRNA-synt_His, 1 hit
PF00458, WHEP-TRS, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF001549, His-tRNA_synth, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00991, WHEP-TRS, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47060, SSF47060, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00442, hisS, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50862, AA_TRNA_LIGASE_II, 1 hit
PS00762, WHEP_TRS_1, 1 hit
PS51185, WHEP_TRS_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: E9QI36-1) [UniParc]FASTAAdd to basket
Also known as: Hars-0021 Publication

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADKAQLQEA IKTQGEVVRK LKSEKASKEQ IDEEVARLLQ LKAQLGGDEG
60 70 80 90 100
KHVFVLKTAK GTRDYNPKQM AIREKVFNII INCFKRHGAE TIDSPVFELK
110 120 130 140 150
ETLTGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKITNIKRY
160 170 180 190 200
HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG QYDAMIPDAE CLKLVYEILS
210 220 230 240 250
ELDLGDFRIK VNDRRILDGM FAICGVPDEK FRTICSTVDK LDKLAWEEVK
260 270 280 290 300
KEMVNEKGLS EEVADRIRDY VSMQGGKDLA ERLLQDPKLS QSKQACAGIT
310 320 330 340 350
DMKLLFSYLE LFQITDKVVF DLSLARGLDY YTGVIYEAIL TQANPAPAST
360 370 380 390 400
PAEQNGAEDA GVSVGSVAGG GRYDGLVGMF DPKGRKVPCV GVSIGIERVF
410 420 430 440 450
SIMEQKAELS SEKVRTTETQ VLVASAQKNL LEERLKLTAE LWNAGIKAEV
460 470 480 490 500
LYKKNPKLLS QLQHCEDTGI PLVAILGEQE LKDGVVKLRN VASREEVDVP
510
RAELVDEVKK RTS
Length:513
Mass (Da):57,585
Last modified:January 20, 2016 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE2EC31D3AC53C113
GO
Isoform 2 (identifier: E9QI36-2) [UniParc]FASTAAdd to basket
Also known as: Hars-0011 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     3-29: DKAQLQEAIKTQGEVVRKLKSEKASKE → ALGLVSMRLCAGLMGRRSAVRLHSLRVCSGMTIS

Show »
Length:520
Mass (Da):58,146
Checksum:iB1C5331AB2924487
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0604653 – 29DKAQL…KASKE → ALGLVSMRLCAGLMGRRSAV RLHSLRVCSGMTIS in isoform 2. Add BLAST27

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
BX890575 Genomic DNA No translation available.

NCBI Reference Sequences

More...
RefSeqi
NP_001289185.1, NM_001302256.1 [E9QI36-2]
NP_001289191.1, NM_001302262.1 [E9QI36-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSDART00000141471; ENSDARP00000123163; ENSDARG00000003693 [E9QI36-2]
ENSDART00000142158; ENSDARP00000113894; ENSDARG00000003693 [E9QI36-1]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
447847

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dre:447847

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX890575 Genomic DNA No translation available.
RefSeqiNP_001289185.1, NM_001302256.1 [E9QI36-2]
NP_001289191.1, NM_001302262.1 [E9QI36-1]

3D structure databases

SMRiE9QI36
ModBaseiSearch...

Proteomic databases

PaxDbiF1Q5D5
PeptideAtlasiE9QI36

Genome annotation databases

EnsembliENSDART00000141471; ENSDARP00000123163; ENSDARG00000003693 [E9QI36-2]
ENSDART00000142158; ENSDARP00000113894; ENSDARG00000003693 [E9QI36-1]
GeneIDi447847
KEGGidre:447847

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
15115
ZFINiZDB-GENE-040912-152, hars

Phylogenomic databases

eggNOGiKOG1936, Eukaryota
GeneTreeiENSGT00390000005922
HOGENOMiCLU_025113_4_2_1
InParanoidiF1Q5D5
OMAiCDFDFIG
OrthoDBi1065556at2759
TreeFamiTF300652

Miscellaneous databases

Protein Ontology

More...
PROi
PR:E9QI36

Gene expression databases

BgeeiENSDARG00000003693, Expressed in somite and 34 other tissues
ExpressionAtlasiE9QI36, baseline and differential

Family and domain databases

CDDicd00773, HisRS-like_core, 1 hit
cd00859, HisRS_anticodon, 1 hit
Gene3Di3.40.50.800, 1 hit
HAMAPiMF_00127, His_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR006195, aa-tRNA-synth_II
IPR004154, Anticodon-bd
IPR036621, Anticodon-bd_dom_sf
IPR015807, His-tRNA-ligase
IPR041715, HisRS-like_core
IPR004516, HisRS/HisZ
IPR033656, HisRS_anticodon
IPR009068, S15_NS1_RNA-bd
IPR000738, WHEP-TRS_dom
PfamiView protein in Pfam
PF03129, HGTP_anticodon, 1 hit
PF13393, tRNA-synt_His, 1 hit
PF00458, WHEP-TRS, 1 hit
PIRSFiPIRSF001549, His-tRNA_synth, 1 hit
SMARTiView protein in SMART
SM00991, WHEP-TRS, 1 hit
SUPFAMiSSF47060, SSF47060, 1 hit
TIGRFAMsiTIGR00442, hisS, 1 hit
PROSITEiView protein in PROSITE
PS50862, AA_TRNA_LIGASE_II, 1 hit
PS00762, WHEP_TRS_1, 1 hit
PS51185, WHEP_TRS_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiHARS1_DANRE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: E9QI36
Secondary accession number(s): F1Q5D5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2020
Last sequence update: January 20, 2016
Last modified: February 10, 2021
This is version 66 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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