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Protein

Helicase with zinc finger domain 2

Gene

Helz2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Helicase that acts as a transcriptional coactivator for a number of nuclear receptors including PPARA, PPARG, THRA, THRB and RXRA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 187C2H2-typeAdd BLAST21
Zinc fingeri289 – 311C2H2-type; atypicalAdd BLAST23
Nucleotide bindingi790 – 797ATPSequence analysis8
Nucleotide bindingi2470 – 2477ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Helicase, Hydrolase
Biological processTranscription, Transcription regulation
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase with zinc finger domain 2 (EC:3.6.4.-)
Alternative name(s):
PPAR-gamma DNA-binding domain-interacting protein 1
Short name:
PDIP1
Short name:
PPAR-gamma DBD-interacting protein 1
Gene namesi
Name:Helz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2385169 Helz2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004243551 – 2947Helicase with zinc finger domain 2Add BLAST2947

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2381Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

EPDiE9QAM5
MaxQBiE9QAM5
PaxDbiE9QAM5
PRIDEiE9QAM5

PTM databases

iPTMnetiE9QAM5
PhosphoSitePlusiE9QAM5

Expressioni

Developmental stagei

In 3T3-L1 cell line, highly expressed before differentiation with a slight decreased after the induction of adipocyte differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000027580 Expressed in 209 organ(s), highest expression level in lymph node
ExpressionAtlasiE9QAM5 baseline and differential
GenevisibleiE9QAM5 MM

Interactioni

Subunit structurei

Interacts with PPARA (via DNA-binding domain) and PPARG; the interaction stimulates the transcriptional activity of PPARA and PPARG. Interacts with THRAP3; the interaction is direct and HELZ2 and THRAP3 synergistically enhance the transcriptional activity of PPARG. It is probably part of the peroxisome proliferator activated receptor alpha interacting complex (PRIC).1 Publication

Protein-protein interaction databases

BioGridi230806, 1 interactor
STRINGi10090.ENSMUSP00000104459

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni809 – 1290Interaction with THRAP3By similarityAdd BLAST482
Regioni2413 – 2947Interaction with THRAP3By similarityAdd BLAST535

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi913 – 916DEAA box4
Motifi1306 – 1310LXXLL motif 15
Motifi1348 – 1352LXXLL motif 25
Motifi1403 – 1407LXXLL motif 35
Motifi2240 – 2244LXXLL motif 45
Motifi2525 – 2529LXXLL motif 55

Domaini

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 187C2H2-typeAdd BLAST21
Zinc fingeri289 – 311C2H2-type; atypicalAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1804 Eukaryota
KOG2102 Eukaryota
COG0557 LUCA
COG1112 LUCA
GeneTreeiENSGT00800000124068
HOGENOMiHOG000231856
HOVERGENiHBG080465
OMAiRVMIPID

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031191 HELZ2
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
IPR001900 RNase_II/R
IPR036236 Znf_C2H2_sf
PANTHERiPTHR10887:SF428 PTHR10887:SF428, 1 hit
PfamiView protein in Pfam
PF00773 RNB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM00955 RNB, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
SSF52540 SSF52540, 2 hits
SSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

E9QAM5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASVGCSLRS ASTSATNGPS LAGLCAKVDL YLGCSRCTQC LNESTYILRE
60 70 80 90 100
VEHTCPREIL LARFKQAAES KIWRKVGRRP SFPTPMRYQV CHYYRPGLGC
110 120 130 140 150
RRHWNRCTFA RSPEEALVWT FELKNNLPRL KLKEAVQGTR APDRLQTPAD
160 170 180 190 200
TIRAEFGGHF QLLCAICFTC CPPCLCPVDP RGHCPKHQIC PTLLIHVIVE
210 220 230 240 250
GLKRQFVEVR PLPQRRHPLN YCMYVGRGVP CRHGASRCEY AHSAVEMAVW
260 270 280 290 300
KAEQLDGLQR GDLLTYPLFG ENKWKASPNP NPPVTKLYCH ACLVTCNSQE
310 320 330 340 350
AFENHCSSLE HAQMVAFDQA VPWKHRAPPM GLSKFDLCPR PDLCEHGEVC
360 370 380 390 400
IKAHSKQELQ EWVQRAQDME LREQAAWQDG LVPYQARLLA EYQRSSKEVS
410 420 430 440 450
VMAETIRGVS VTCHPPPVHQ AQEKIQHQWV FTIHSEDPLL HVALLNQEPG
460 470 480 490 500
AAFSLVAPSL PPHQLYAQGK HFCVQSSPAQ YKVGVLVQAV AFGSFEQWVV
510 520 530 540 550
FDFGRRPVLL QKLKLQLGQT HSQGLNGKPA PSHPQELECW HTGNRHVVLE
560 570 580 590 600
VDWTPEQEAL MAKYKLPSLA LEFNQIVPDW GPISRSNYRQ RMHKFLYEEE
610 620 630 640 650
AAQQQLVAKL AMKGQVSLKT ALETPALGML FAPPGALYAK VPFHSSLLPD
660 670 680 690 700
TDQGFLLSRA VSTALVAPVP APNSTVYQVR LEARASSDHA LWLLLPARCC
710 720 730 740 750
MALGLQAQDS PILEVQFQID PMTFRFWHQA VDALLEEHLV VPDLPACTLP
760 770 780 790 800
HPWPTPPSFR GNHKQKLAVG LIAGRRPEGT KHIPPLLIYG PFGTGKTYTL
810 820 830 840 850
AMAALEVVQQ PHTKVLICTH TNSAADIYIR EYFHDYVSSG HPEATPLRVM
860 870 880 890 900
YADRPPRQTD PTTLQYCCLT EDRQAFRPPT GPELVHHRLV VTTTSQAREL
910 920 930 940 950
QVPAGFFSHI FIDEAAQMLE CEALIPLSYA LSLTRVVLAG DHMQVTPRLF
960 970 980 990 1000
SVPRDKSARH TLLHRLFLYY QQEAHKIAQQ SRIIFHENYR STAAIINFVS
1010 1020 1030 1040 1050
HHFYLAKGNP IQASGKVPRH PQHYPLMFCH VAGSPEQDMS MTSWLNSAEV
1060 1070 1080 1090 1100
TQVVEKVREI YNTWPHCWGP REQRHICAVS HGAQVSALRQ ELRRRNLGEV
1110 1120 1130 1140 1150
SVGSFEILPG REFRVVVLSS VHNRNSLLSP GAPTSEFFTE PRVLNTVMTR
1160 1170 1180 1190 1200
AQSQLVAVGD AVALCSSGAC RNLWRSFIRE CIEHHSAFPE ELSLEQIEQG
1210 1220 1230 1240 1250
VAQRQNWASL TLKARGPETE QKSMAQGPQR LIAEGTMVTV KAETRAEAAA
1260 1270 1280 1290 1300
KAQTAAVAAE DTASGNSASR DAAAEVSTLE GGMSEEDSES DFWPSDWELN
1310 1320 1330 1340 1350
ADDAILKELL DESQQVTVTV REDGLLDTVV CSAPQKAREY TNLPSSVLWK
1360 1370 1380 1390 1400
FLRSNSKQFR RCSFLQETFE RALATPLDDM ASSPIQVRGR LNCGMAFTGD
1410 1420 1430 1440 1450
EVLVQILGPA GDDRCVPGSL QGRVMGVLKR RRHELAFVCR MDEWDPRIMI
1460 1470 1480 1490 1500
PINGSVTKIF VAEMKDPQQV PIHRLIQGQV QRVRHETLKP EDRSTRLFWV
1510 1520 1530 1540 1550
RIVLWRERFY YPLGIVLEVL PKAITWEQGL YILDLEHGLK AHTPDPASVS
1560 1570 1580 1590 1600
KALQRYRSEL NTAAGHREDY RHFLTFTVDP QGACNLDDAL SVRDLGPVYE
1610 1620 1630 1640 1650
VAVHIADVAS LVPKDGALDV EARQQGTVFY APNREPVLML PASLCQDALS
1660 1670 1680 1690 1700
LLPGQDRLAI SLFLTMEKGG GQIKSLRFAP SIIRSDRQLS YEEAEELIKR
1710 1720 1730 1740 1750
HPGAGLELPA HLDSVEACVV AACYFSWMLR RQRLSAACYY EPPDEDSVLG
1760 1770 1780 1790 1800
FRTAHIMVQE YMIQFNSHVA EFLVSNKHTQ TLTPLRWQPT PSRQQLDSVF
1810 1820 1830 1840 1850
KKYRGLVPLS LHLCHHSNTD YTPNKQLHLL TSLWKQVQLA AGTQDYSQMV
1860 1870 1880 1890 1900
DLIAADDMHP SLAPACLDLR RALGRSVFGR SSQGKQQPAV HHSLQVDWYT
1910 1920 1930 1940 1950
WATSPIRRYL DVVLQRLILL ALGHRGSTYS NRDIDGLCLD FSRQYASAQS
1960 1970 1980 1990 2000
YQRRAYSLHL AIQLKSQPQN KLGFVVDVEM GARCFKVLFP INRETLPDPC
2010 2020 2030 2040 2050
PIHYHSLQLA EHPQELVSQT GVRLVWRRRM YSVQASKLPL PLLGTSLDPH
2060 2070 2080 2090 2100
TQTVDAALWM KLLMLLKEQR WPEIAALIQE QDKRFHPREK VKIHQSRCGH
2110 2120 2130 2140 2150
FVEVVYELGS GDTLQVQLGS SLQRGFLAPT LKLWTVVPGF SLCLEHMERP
2160 2170 2180 2190 2200
GDCFSSHVHQ ALQDQYLQVG EYSGAWGPRC ALESLTNAVT ENDSIVLHDV
2210 2220 2230 2240 2250
HISWDTSQGQ LQGTFQLEAA FLQEKCINIH FGCCYLCIRL EGLPLPLDSS
2260 2270 2280 2290 2300
LPGPSGLGPF LNIDPNTYTW VAHGLSGDWD HELAGGDWDQ ENVDDRQEAP
2310 2320 2330 2340 2350
KQVYFLIHHM TMEKVPEEVL RPSARFTVEV LSKQLPDLRK EEAVRQLKTA
2360 2370 2380 2390 2400
SPLVISIALG LPIPEIRWPI SGPRRLVSEL RWPIPGPRRP VSEPHRPMSG
2410 2420 2430 2440 2450
PCGPISEPCR SIPEPCRGNW PRQHSFHKAS TSRFLERQNY NIPAGHHKLN
2460 2470 2480 2490 2500
QSQDRAVRSA LQKQFTVIQG PPGTGKTVVG FHIVYWFHRS NQEQMPTDSS
2510 2520 2530 2540 2550
PSGEEQLGGP CVLYCGPSNK SVDVLGGLLL RRKTEMKPLR VYGEQAEATE
2560 2570 2580 2590 2600
FPLPGVSNRS LFGKTSQEGR PNQSLRSITL HHRIRQAPNP YAAEIRKFDA
2610 2620 2630 2640 2650
QLREGKIFSK EDLRVYRRVL GKARKHELER HSVILCTCSC AASKSLKILN
2660 2670 2680 2690 2700
VRQILIDEAG MATEPETLIP LVCFSKTVEK VVLLGDHKQL RPVVKSEQLQ
2710 2720 2730 2740 2750
SLGMDRSLFE RYHRDAIMLD TQYRMHKDIC SFPSVEFYGG KLKTWSDLRR
2760 2770 2780 2790 2800
LPSILGHTGK PSCSVIFGSV QGHEQKLLVS TEDGNENSRA NPEEVTQVVR
2810 2820 2830 2840 2850
IIKQLTLDRT VDPKDIAVLT PYNAQAAAIS RGLMQRGVTG VTVTSITKSQ
2860 2870 2880 2890 2900
GSEWRYVIVS TVRTCPRSDV DQRPTKSWLK KFLGFVVDPH QVNVAITRAQ
2910 2920 2930 2940
EALCIIGDHL LLRCCPLWHR LLDFCEAQHS LVSAEKVRVQ RKSALSS
Length:2,947
Mass (Da):331,563
Last modified:April 5, 2011 - v1
Checksum:i7B82DF8713B34988
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AS03A2AS03_MOUSE
Helicase with zinc finger domain 2
Helz2 BC006779
2,970Annotation score:
A2AS05A2AS05_MOUSE
Helicase with zinc finger domain 2
Helz2 BC006779
2,903Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348E → G in BAH20441 (Ref. 1) Curated1
Sequence conflicti407R → C in BAH20441 (Ref. 1) Curated1
Sequence conflicti472F → S in BAH20441 (Ref. 1) Curated1
Sequence conflicti946T → A in BAH20441 (Ref. 1) Curated1
Sequence conflicti1301A → T in BAH20441 (Ref. 1) Curated1
Sequence conflicti1316V → A in BAH20441 (Ref. 1) Curated1
Sequence conflicti2337D → E in BAH20441 (Ref. 1) Curated1
Sequence conflicti2639S → C in BAH20441 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB201714 mRNA Translation: BAH20441.1
AL845506 Genomic DNA No translation available.
CCDSiCCDS38380.1
RefSeqiNP_898985.2, NM_183162.2
XP_006500662.1, XM_006500599.3
UniGeneiMm.315872

Genome annotation databases

EnsembliENSMUST00000108831; ENSMUSP00000104459; ENSMUSG00000027580
GeneIDi229003
KEGGimmu:229003
UCSCiuc008oln.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB201714 mRNA Translation: BAH20441.1
AL845506 Genomic DNA No translation available.
CCDSiCCDS38380.1
RefSeqiNP_898985.2, NM_183162.2
XP_006500662.1, XM_006500599.3
UniGeneiMm.315872

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230806, 1 interactor
STRINGi10090.ENSMUSP00000104459

PTM databases

iPTMnetiE9QAM5
PhosphoSitePlusiE9QAM5

Proteomic databases

EPDiE9QAM5
MaxQBiE9QAM5
PaxDbiE9QAM5
PRIDEiE9QAM5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108831; ENSMUSP00000104459; ENSMUSG00000027580
GeneIDi229003
KEGGimmu:229003
UCSCiuc008oln.1 mouse

Organism-specific databases

CTDi85441
MGIiMGI:2385169 Helz2

Phylogenomic databases

eggNOGiKOG1804 Eukaryota
KOG2102 Eukaryota
COG0557 LUCA
COG1112 LUCA
GeneTreeiENSGT00800000124068
HOGENOMiHOG000231856
HOVERGENiHBG080465
OMAiRVMIPID

Enzyme and pathway databases

ReactomeiR-MMU-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

Miscellaneous databases

PROiPR:E9QAM5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027580 Expressed in 209 organ(s), highest expression level in lymph node
ExpressionAtlasiE9QAM5 baseline and differential
GenevisibleiE9QAM5 MM

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031191 HELZ2
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
IPR001900 RNase_II/R
IPR036236 Znf_C2H2_sf
PANTHERiPTHR10887:SF428 PTHR10887:SF428, 1 hit
PfamiView protein in Pfam
PF00773 RNB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM00955 RNB, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
SSF52540 SSF52540, 2 hits
SSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiHELZ2_MOUSE
AccessioniPrimary (citable) accession number: E9QAM5
Secondary accession number(s): B9A0U1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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