Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Triadin

Gene

Trdn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. Required for normal skeletal muscle strength (PubMed:19843516). Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.3 Publications

GO - Molecular functioni

  • ion channel binding Source: GO_Central
  • signaling receptor binding Source: InterPro

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels
R-MMU-5578775 Ion homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Triadin
Gene namesi
Name:TrdnImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1924007 Trdn

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47CytoplasmicSequence analysisAdd BLAST47
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 693LumenalSequence analysisAdd BLAST625

Keywords - Cellular componenti

Membrane, Sarcoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Mice appear normal; they are viable and fertile but have reduced muscular strength, due to defects in the structure of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. In mutant mice, about 25% of the triads are in oblique or longitudinal orientation, instead of the normal transversal orientation (PubMed:19843516). Similar structural defects are seen in the heart, leading to impaired excitation-contraction coupling. Mutant mice are subject to stress-induced ventricular tachycardia (PubMed:19383796).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004305621 – 693TriadinAdd BLAST693

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi268InterchainBy similarity
Modified residuei301PhosphoserineBy similarity1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi584N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi655InterchainBy similarity

Post-translational modificationi

Phosphorylated by CaMK2.By similarity
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiE9Q9K5
PaxDbiE9Q9K5
PRIDEiE9Q9K5

PTM databases

iPTMnetiE9Q9K5
PhosphoSitePlusiE9Q9K5

Expressioni

Tissue specificityi

Detected in heart (at protein level). Detected in heart.3 Publications

Gene expression databases

BgeeiENSMUSG00000019787 Expressed in 117 organ(s), highest expression level in tarsal region
ExpressionAtlasiE9Q9K5 baseline and differential
GenevisibleiE9Q9K5 MM

Interactioni

Subunit structurei

Homooligomer of variable subunit number; disulfide-linked. Interacts with CASQ1 and RYR1 in skeletal muscle. Interacts with CASQ2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiE9Q9K5, 1 interactor
STRINGi10090.ENSMUSP00000093436

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi140 – 645Lys-richPROSITE-ProRule annotationAdd BLAST506

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK92 Eukaryota
ENOG410YQIE LUCA
GeneTreeiENSGT00510000049207
HOGENOMiHOG000172367
HOVERGENiHBG014932
InParanoidiE9Q9K5
OMAiKDEKKDY
OrthoDBiEOG091G06WH
TreeFamiTF350396

Family and domain databases

InterProiView protein in InterPro
IPR010798 Triadin
PANTHERiPTHR14106 PTHR14106, 2 hits

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: E9Q9K5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEITAEGNA STTTTVIDNK NGCIPKSPGK VLKRSVTEDI VTTFSSPAAW
60 70 80 90 100
LLVIALIITW SAVAIVMFDL VDYKNFSASS IAKIGSDPLK LVNDAVEETT
110 120 130 140 150
DWIYGFFSLL SDIISSEGDE DDEDADEDID KGEIEEPPLK RKEIHQEKAE
160 170 180 190 200
KEEKPEKKIQ TKASHREREK GKEKLKGEKP EKTATHKEKL EKKERPETKM
210 220 230 240 250
MAKEDKKIKT KEKTEEKAKK EMKVGKQEKV KPTAAKAKET PKTPPKARKK
260 270 280 290 300
DDKEMPAVHE QKDQYAFCRY MIDMFVHGDL KPGQSPVMPP PSLTPSKPAL
310 320 330 340 350
STTALEEKEK EEKKKMEKKD TSDTKKKEKE VKKKSEETTI DGKGKEPGKP
360 370 380 390 400
PETKQMTAKL TTQAAARKDE KKEESKKMRK PTEEQPKGKK QEKKEKHIEP
410 420 430 440 450
AKTPKKEHPG PSEKLKKAKA EQAKEEIAAA STKKALHGKK EEKAKTVEQE
460 470 480 490 500
VKKEKSGKSS SDLKDKEVKK EKSGKSSSDL KDKEPQLKNE EKSKPQVKKE
510 520 530 540 550
AKLASSDKGQ TRKQNITRPE QVIPHVKPEK AEHQEKGHPS IKKDKPKPSS
560 570 580 590 600
KGAPEVPDSG KKKIEKSEKE SKVPTREENL QVYNVTKAEK PGKIPKDSKE
610 620 630 640 650
APASKKDKED SKEAPTSKKD KEDSKDVPHS KKDKEVTDDV SSPKKQTRPI
660 670 680 690
SFFQCVYLNG YNGYGFQFPV TPVQQPGENP GKTNSPGQKQ QEQ
Length:693
Mass (Da):77,844
Last modified:April 5, 2011 - v1
Checksum:i6DD93304D5509F62
GO
Isoform 2 (identifier: E9Q9K5-2) [UniParc]FASTAAdd to basket
Also known as: Cardiac triadin 1

The sequence of this isoform differs from the canonical sequence as follows:
     263-277: DQYAFCRYMIDMFVH → GKHSRRRRQQEVQRE
     278-693: Missing.

Show »
Length:277
Mass (Da):31,438
Checksum:i85CED9D841DE4519
GO
Isoform 3 (identifier: E9Q9K5-3) [UniParc]FASTAAdd to basket
Also known as: Cardiac triadin 2

The sequence of this isoform differs from the canonical sequence as follows:
     284-293: QSPVMPPPSL → IPNNSYCCLS
     294-693: Missing.

Show »
Length:293
Mass (Da):33,090
Checksum:iF0638F453EB3444E
GO
Isoform 4 (identifier: E9Q9K5-4) [UniParc]FASTAAdd to basket
Also known as: Cardiac triadin 3, Trisk 32

The sequence of this isoform differs from the canonical sequence as follows:
     263-282: Missing.
     307-325: EKEKEEKKKMEKKDTSDTK → GKYLVLWCFLPHKYFHYIF
     326-693: Missing.

Show »
Length:305
Mass (Da):34,351
Checksum:iD4B7DA47A8840FF6
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P8G7A0A1W2P8G7_MOUSE
Triadin
Trdn
286Annotation score:
A0A1W2P7C3A0A1W2P7C3_MOUSE
Triadin
Trdn
205Annotation score:
A0A1W2P6F3A0A1W2P6F3_MOUSE
Triadin
Trdn
56Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34R → K in AAL33876 (PubMed:11707337).Curated1
Sequence conflicti34R → K in AAL33877 (PubMed:11707337).Curated1
Sequence conflicti34R → K in AAL33878 (PubMed:11707337).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056795263 – 282Missing in isoform 4. CuratedAdd BLAST20
Alternative sequenceiVSP_056796263 – 277DQYAF…DMFVH → GKHSRRRRQQEVQRE in isoform 2. CuratedAdd BLAST15
Alternative sequenceiVSP_056797278 – 693Missing in isoform 2. CuratedAdd BLAST416
Alternative sequenceiVSP_056798284 – 293QSPVMPPPSL → IPNNSYCCLS in isoform 3. 10
Alternative sequenceiVSP_056799294 – 693Missing in isoform 3. CuratedAdd BLAST400
Alternative sequenceiVSP_056800307 – 325EKEKE…TSDTK → GKYLVLWCFLPHKYFHYIF in isoform 4. CuratedAdd BLAST19
Alternative sequenceiVSP_056801326 – 693Missing in isoform 4. CuratedAdd BLAST368

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223415 mRNA Translation: AAL33876.1
AF223416 mRNA Translation: AAL33877.1
AF223417 mRNA Translation: AAL33878.1
AC109214 Genomic DNA No translation available.
AC153965 Genomic DNA No translation available.
AC153966 Genomic DNA No translation available.
AC157017 Genomic DNA No translation available.
AK009816 mRNA Translation: BAB26520.1
CCDSiCCDS48532.1 [E9Q9K5-1]
RefSeqiNP_084002.2, NM_029726.2 [E9Q9K5-1]
UniGeneiMm.338508

Genome annotation databases

EnsembliENSMUST00000095762; ENSMUSP00000093436; ENSMUSG00000019787 [E9Q9K5-1]
ENSMUST00000217779; ENSMUSP00000151583; ENSMUSG00000019787 [E9Q9K5-3]
ENSMUST00000219931; ENSMUSP00000151999; ENSMUSG00000019787 [E9Q9K5-4]
ENSMUST00000219982; ENSMUSP00000152042; ENSMUSG00000019787 [E9Q9K5-2]
GeneIDi76757
KEGGimmu:76757
UCSCiuc007etz.2 mouse [E9Q9K5-2]
uc007eua.2 mouse [E9Q9K5-3]
uc007eub.2 mouse [E9Q9K5-4]
uc011xcl.1 mouse [E9Q9K5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223415 mRNA Translation: AAL33876.1
AF223416 mRNA Translation: AAL33877.1
AF223417 mRNA Translation: AAL33878.1
AC109214 Genomic DNA No translation available.
AC153965 Genomic DNA No translation available.
AC153966 Genomic DNA No translation available.
AC157017 Genomic DNA No translation available.
AK009816 mRNA Translation: BAB26520.1
CCDSiCCDS48532.1 [E9Q9K5-1]
RefSeqiNP_084002.2, NM_029726.2 [E9Q9K5-1]
UniGeneiMm.338508

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiE9Q9K5, 1 interactor
STRINGi10090.ENSMUSP00000093436

PTM databases

iPTMnetiE9Q9K5
PhosphoSitePlusiE9Q9K5

Proteomic databases

MaxQBiE9Q9K5
PaxDbiE9Q9K5
PRIDEiE9Q9K5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095762; ENSMUSP00000093436; ENSMUSG00000019787 [E9Q9K5-1]
ENSMUST00000217779; ENSMUSP00000151583; ENSMUSG00000019787 [E9Q9K5-3]
ENSMUST00000219931; ENSMUSP00000151999; ENSMUSG00000019787 [E9Q9K5-4]
ENSMUST00000219982; ENSMUSP00000152042; ENSMUSG00000019787 [E9Q9K5-2]
GeneIDi76757
KEGGimmu:76757
UCSCiuc007etz.2 mouse [E9Q9K5-2]
uc007eua.2 mouse [E9Q9K5-3]
uc007eub.2 mouse [E9Q9K5-4]
uc011xcl.1 mouse [E9Q9K5-1]

Organism-specific databases

CTDi10345
MGIiMGI:1924007 Trdn

Phylogenomic databases

eggNOGiENOG410IK92 Eukaryota
ENOG410YQIE LUCA
GeneTreeiENSGT00510000049207
HOGENOMiHOG000172367
HOVERGENiHBG014932
InParanoidiE9Q9K5
OMAiKDEKKDY
OrthoDBiEOG091G06WH
TreeFamiTF350396

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels
R-MMU-5578775 Ion homeostasis

Miscellaneous databases

PROiPR:E9Q9K5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019787 Expressed in 117 organ(s), highest expression level in tarsal region
ExpressionAtlasiE9Q9K5 baseline and differential
GenevisibleiE9Q9K5 MM

Family and domain databases

InterProiView protein in InterPro
IPR010798 Triadin
PANTHERiPTHR14106 PTHR14106, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRDN_MOUSE
AccessioniPrimary (citable) accession number: E9Q9K5
Secondary accession number(s): Q8VIN7
, Q8VIN8, Q8VIN9, Q9CV36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again