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Protein

PR domain zinc finger protein 15

Gene

Prdm15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Plays also a role in induced pluripotent stem cells (iPSCs) reprogramming. Involved in early embryo development.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri402 – 424C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri434 – 457C2H2-type 2PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri468 – 486C2H2-type 3; degeneratePROSITE-ProRule annotationAdd BLAST19
Zinc fingeri495 – 517C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri522 – 544C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri571 – 593C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri598 – 620C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri661 – 684C2H2-type 8PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri689 – 711C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri725 – 747C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri753 – 775C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri781 – 803C2H2-type 12PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri809 – 831C2H2-type 13PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri837 – 859C2H2-type 14PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri865 – 888C2H2-type 15PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding, Methyltransferase, Repressor, Transferase
Biological processTranscription, Transcription regulation
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PR domain zinc finger protein 15Curated (EC:2.1.1.-Curated)
Alternative name(s):
PR domain-containing protein 15Curated
Gene namesi
Name:Prdm15Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1930121 Prdm15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice die before birth and show early postimplantation developmental defect. Display reduced embryonic stem cells (ESCs) proliferation and self-renewal capacity. Show altered transcription of naive pluripotency and self-renewal modulator genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004447181 – 1174PR domain zinc finger protein 15Add BLAST1174

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki552Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiE9Q8T2
PaxDbiE9Q8T2
PRIDEiE9Q8T2

Expressioni

Tissue specificityi

Expressed in embryonic stem cells (ESCs) (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000014039 Expressed in 248 organ(s), highest expression level in pituitary gland
ExpressionAtlasiE9Q8T2 baseline and differential
GenevisibleiE9Q8T2 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093533

Structurei

3D structure databases

SMRiE9Q8T2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 185SETPROSITE-ProRule annotationAdd BLAST111

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri402 – 424C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri434 – 457C2H2-type 2PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri468 – 486C2H2-type 3; degeneratePROSITE-ProRule annotationAdd BLAST19
Zinc fingeri495 – 517C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri522 – 544C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri571 – 593C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri598 – 620C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri661 – 684C2H2-type 8PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri689 – 711C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri725 – 747C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri753 – 775C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri781 – 803C2H2-type 12PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri809 – 831C2H2-type 13PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri837 – 859C2H2-type 14PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri865 – 888C2H2-type 15PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00920000149092
HOGENOMiHOG000115685
HOVERGENiHBG053669
InParanoidiE9Q8T2
OrthoDBiEOG091G02KC
TreeFamiTF331419

Family and domain databases

InterProiView protein in InterPro
IPR001214 SET_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 7 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 17 hits
SUPFAMiSSF57667 SSF57667, 7 hits
PROSITEiView protein in PROSITE
PS50280 SET, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 16 hits
PS50157 ZINC_FINGER_C2H2_2, 15 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: E9Q8T2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCPPTIWEKG GQVGARWSLR APEVSAMAED GSEEIMFIWC EDCSQYHDSE
60 70 80 90 100
CPELGPVVMV KDSFVLSRAR SSLPSNLEIR RLDDGAEGVF AVTQLVKRTQ
110 120 130 140 150
FGPFESRRVA KWEKESAFPL KVFQKDGHPV CFDTSNEDDC NWMMLVRPAL
160 170 180 190 200
EPGHQNLTAY QHGSDVYFTT SKDIPAGTEL RVWYAAFYAK KMDKPMLKQA
210 220 230 240 250
CSSVQAAGTP EPSVSVEPER GQWVCKVCSN TFLELQLLNE HLLGHLEQAK
260 270 280 290 300
SLPAGGQQHE AASEKEPDAP RMEPPTAAES KSIQSVMVTK EPKKKPRRGR
310 320 330 340 350
KPKASKVEQP LVIIKDKEPS EHVAEIITEI PPDEPVSATP DERIMELVLG
360 370 380 390 400
KLAAPTNEAS SVPKFPHHPS STIALKRGLV LSSRHGVRRK LVRQLGEHKR
410 420 430 440 450
IHQCGTCSKV FQNSSNLSRH VRSHGECAHG DKLFKCEECS KLFSRKESLK
460 470 480 490 500
QHVSYKHSRN EVDGEYRYRC GSCGKTFRME SALEFHNCRT DDKTFQCEMC
510 520 530 540 550
FRFFSTNSNL SKHKKKHGDK KFACEVCSKM FYRKDVMLDH QRRHLDGVRR
560 570 580 590 600
VKREDLEASG ESLVRYKKEP SGCPVCGKVF SCRSNMNKHL LTHGDKKYTC
610 620 630 640 650
EICGRKFFRV DVLRDHIHVH FKDIALMDDH QREEFIGKIG ISSEENDDNS
660 670 680 690 700
DESADSEPHK YSCKRCQLTF GRGKEYLKHI MEVHKEKGHG CSICHRRFAL
710 720 730 740 750
KATYHAHMVI HRENLPDPNV QKYIHPCEIC GRIFNSIGNL ERHKLIHTGV
760 770 780 790 800
KSHACEQCGK SFARKDMLKE HMRVHDNIRE YLCAECGKGM KTKHALRHHM
810 820 830 840 850
KLHKGIKEYE CKECHRKFAQ KVNMLKHYKR HTGIKDFMCE LCGKTFSERN
860 870 880 890 900
TMETHKLIHT VGKQWTCSVC DKKYVTEYML QKHVQLTHDK VEAQSCQLCG
910 920 930 940 950
TKVSTRASMS RHMRRKHPEV LAVRIDDLDH LPETTTIDAS SIGIVQPALG
960 970 980 990 1000
LEQEELAEGK HGKAAKRSHK RKQKPEEEAG APVPEDTTFS EYPEKEPEFT
1010 1020 1030 1040 1050
GSVGDETNSA VQSIQQVVVT LGDPNVTAPS SSVGLTNITV TPITTAAGTQ
1060 1070 1080 1090 1100
FTNLQPVAVG HLTNPDRQLQ LDNSILTVTF DTVSGSAMLH NRQNDVQIHP
1110 1120 1130 1140 1150
QPEATNPQSV AHFINLTTLV NSITPLGNQL SEQHPLTWRA VPQTDVLQPP
1160 1170
QAPAAPQQAV QPQVQNEQQQ MYSY
Length:1,174
Mass (Da):132,870
Last modified:April 5, 2011 - v1
Checksum:i2C12468F8E056D76
GO
Isoform 2 (identifier: E9Q8T2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: Missing.

Show »
Length:1,148
Mass (Da):130,061
Checksum:i1FF878205B7109D9
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RIL7D6RIL7_MOUSE
PR domain zinc finger protein 15
Prdm15
474Annotation score:
D3Z7Q9D3Z7Q9_MOUSE
PR domain zinc finger protein 15
Prdm15
110Annotation score:
D3YYH8D3YYH8_MOUSE
PR domain zinc finger protein 15
Prdm15
85Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti298R → I in BAE26081 (PubMed:16141072).Curated1
Sequence conflicti609R → L in BAE26081 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0596471 – 26Missing in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144820 mRNA Translation: BAE26081.1
AC121560 Genomic DNA No translation available.
CCDSiCCDS49926.1 [E9Q8T2-1]
RefSeqiNP_659038.2, NM_144789.2 [E9Q8T2-1]
XP_006522932.1, XM_006522869.3 [E9Q8T2-2]
UniGeneiMm.328741

Genome annotation databases

EnsembliENSMUST00000095849; ENSMUSP00000093533; ENSMUSG00000014039 [E9Q8T2-1]
ENSMUST00000121584; ENSMUSP00000113791; ENSMUSG00000014039 [E9Q8T2-2]
GeneIDi114604
UCSCiuc012ajc.1 mouse [E9Q8T2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144820 mRNA Translation: BAE26081.1
AC121560 Genomic DNA No translation available.
CCDSiCCDS49926.1 [E9Q8T2-1]
RefSeqiNP_659038.2, NM_144789.2 [E9Q8T2-1]
XP_006522932.1, XM_006522869.3 [E9Q8T2-2]
UniGeneiMm.328741

3D structure databases

SMRiE9Q8T2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000093533

Proteomic databases

MaxQBiE9Q8T2
PaxDbiE9Q8T2
PRIDEiE9Q8T2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095849; ENSMUSP00000093533; ENSMUSG00000014039 [E9Q8T2-1]
ENSMUST00000121584; ENSMUSP00000113791; ENSMUSG00000014039 [E9Q8T2-2]
GeneIDi114604
UCSCiuc012ajc.1 mouse [E9Q8T2-1]

Organism-specific databases

CTDi63977
MGIiMGI:1930121 Prdm15

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00920000149092
HOGENOMiHOG000115685
HOVERGENiHBG053669
InParanoidiE9Q8T2
OrthoDBiEOG091G02KC
TreeFamiTF331419

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014039 Expressed in 248 organ(s), highest expression level in pituitary gland
ExpressionAtlasiE9Q8T2 baseline and differential
GenevisibleiE9Q8T2 MM

Family and domain databases

InterProiView protein in InterPro
IPR001214 SET_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00096 zf-C2H2, 7 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 17 hits
SUPFAMiSSF57667 SSF57667, 7 hits
PROSITEiView protein in PROSITE
PS50280 SET, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 16 hits
PS50157 ZINC_FINGER_C2H2_2, 15 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPRD15_MOUSE
AccessioniPrimary (citable) accession number: E9Q8T2
Secondary accession number(s): E9Q6A1, Q3UML7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 18, 2018
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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