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Protein

Rho guanine nucleotide exchange factor 5

Gene

Arhgef5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:19713215, PubMed:21525037). Strongly activates RHOA (PubMed:19713215, PubMed:21525037). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (PubMed:19713215). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:21525037). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (By similarity). Required for SRC-induced podosome formation (PubMed:21525037). Involved in positive regulation of immature dendritic cell migration (PubMed:19713215).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 5Imported
Gene namesi
Name:Arhgef5Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1858952 Arhgef5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Chemotaxis of macrophages, T and B lymphocytes and bone marrow-derived mature dendritic cells from mutant mice is not affected but chemotaxis of immature dendritic cells is abolished.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004351431 – 1581Rho guanine nucleotide exchange factor 5Add BLAST1581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei446PhosphoserineBy similarity1
Modified residuei953PhosphoserineCombined sources1
Modified residuei969PhosphoserineBy similarity1
Modified residuei1029PhosphoserineBy similarity1
Modified residuei1110PhosphoserineCombined sources1

Post-translational modificationi

Activation of SRC induces tyrosine phosphorylation of ARHGEF5.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiE9Q7D5
PaxDbiE9Q7D5
PeptideAtlasiE9Q7D5
PRIDEiE9Q7D5

PTM databases

iPTMnetiE9Q7D5
PhosphoSitePlusiE9Q7D5

Expressioni

Gene expression databases

BgeeiENSMUSG00000033542 Expressed in 225 organ(s), highest expression level in placenta labyrinth
ExpressionAtlasiE9Q7D5 baseline and differential
GenevisibleiE9Q7D5 MM

Interactioni

Subunit structurei

Interacts with SRC (PubMed:21525037). Forms a ternary complex with SRC and the PI3K 85 kDa subunit (PubMed:21525037). Interacts with and is activated by the heterodimer formed by GNB1 and GNG2 (PubMed:19713215). Interacts with ODAM (via C-terminus) (By similarity). Interacts with RHOA (PubMed:19713215).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031750

Structurei

3D structure databases

SMRiE9Q7D5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1158 – 1342DHPROSITE-ProRule annotationAdd BLAST185
Domaini1375 – 1488PHSequence analysisAdd BLAST114
Domaini1494 – 1555SH3PROSITE-ProRule annotationAdd BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 333Glu-richPROSITE-ProRule annotationAdd BLAST172
Compositional biasi775 – 843Pro-richPROSITE-ProRule annotationAdd BLAST69

Domaini

The PH domain binds to phosphoinositides and is essential for podosome formation.1 Publication

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3523 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00550000074493
HOGENOMiHOG000007536
HOVERGENiHBG058673
InParanoidiE9Q7D5
KOiK20684
OMAiWPHRRDS
OrthoDBiEOG091G00P4
TreeFamiTF342609

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR029212 ARHGEF5/35_N
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF15441 ARHGEF5_35, 1 hit
PF00621 RhoGEF, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50002 SH3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

E9Q7D5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEAEEPEYGV STEVPDIEEL KTIPEGIMRS SQIPALDPEA QEDRDPSYKW
60 70 80 90 100
TDGHRPVMNQ SKVLRDMGDH TPNSMAIFFK KESSDMETSQ EILLAEACNT
110 120 130 140 150
PDQQEAVIQS LKDRLSRTIA APELLACAVQ EEWLDIPSKL DNRVGAELQS
160 170 180 190 200
ELMSLTLAVS KEKEEEETSP DTSIPRGSWP PCKTHPGETE QTQGSGSELL
210 220 230 240 250
RQGKQLQLEA TQENQGQEGF LQSQEAQGLE EQEGQEVEIQ EEGTLNEGIC
260 270 280 290 300
FGGLLGEQEE VEEGFNGNEE EQKQGQIQSY MLLGGQWENE GLSGELEGLN
310 320 330 340 350
YSERGQENRE RRVWVLRDSE EEGQDQESRE VEERRVATQY TENQRLVEKS
360 370 380 390 400
EIVKRKQRDH DQTGKVMPVR DQKEVVDSGD RVQGNGDSGG QTAVEGSRPG
410 420 430 440 450
EDSKPSLPVA SVDPEVLSPG TLFPGISSSV ADIPQIQKEP VCEELSPQAP
460 470 480 490 500
ALEPTEWSHQ PISPPASFAP EESLDNRTHN SQQEEFRLRK GIEVVSASTS
510 520 530 540 550
VAPSGTRDSP PFSPPNVFSS TATLSPVSSS VILPEETPTA SASADTPHHC
560 570 580 590 600
GPCETPPLPA KSSRYPCATS DTANPHSPLS SYTGVTQHLR SNSFPGSHRT
610 620 630 640 650
EQTPDSLGMS LSFSHLELPQ RPPKPAIYGS LTPRRNRRSR DGIVFSDSST
660 670 680 690 700
ALFALKQDSE EFTSNPERPS SPHGSPTWGS PQNSAFAIGS PANVSSPPTV
710 720 730 740 750
SMDMTIREAL LPIPPEKRHS YSHIVERDGL LHEVASTLKR HSHPPPLTLS
760 770 780 790 800
SGLHRSSKGS FSLVPDSTVA RQHRPLPSTP ESPNHTQTSI PSRLRYNKPL
810 820 830 840 850
PPTPDMPEFY HPSISSSYIS RMYRPLPPVP IIDPSSEPPP LPPKSRGRSK
860 870 880 890 900
SIQGGVIHSG GQAKPRPNNQ DWTASTLSVG RTSWPPATGR STESLPLTSR
910 920 930 940 950
CNNEVSPGLA FSNMTNLLSP SSPTTPWIPD LQRPTTKDES GLTEESEPPV
960 970 980 990 1000
RGSFRRSAPQ EEFNNTRRSA LGSRKNSEKP LHHQLEKASS WPHRRDPART
1010 1020 1030 1040 1050
SESSSEQVVL GQVPNKQKGW NRQGLRRPSI LPESSSDLRN PAAGRLPGSS
1060 1070 1080 1090 1100
DSVVFREKKP KEGMGGFSRR CSKLISSQLL YQEYSDVVLN KEIQSQQRLD
1110 1120 1130 1140 1150
SLAEPHGLSS PRHRRKALVS SDSYLQRLSM ASSGSLWQEI PVVRNSTVLL
1160 1170 1180 1190 1200
SMTHEDQKLQ EAKFELIVSE ASYLRSLNIA VDHFQHSAQL RGTLSNQDHQ
1210 1220 1230 1240 1250
WLFSRLQDVR DVSTTFLSDL EENFENNIFS FQVCDVVLNH AADFHRVYLP
1260 1270 1280 1290 1300
YVTNQTYQER TFQSLMNSNS SFREVLEKLE SDPICQRLSL KSFLILPFQR
1310 1320 1330 1340 1350
ITRLKLLLQN ILKRTQPGSS EEAEATKAHH ALEKLIRDCN SNVQRMRRTE
1360 1370 1380 1390 1400
ELIYLSQKIE FECKIFPLIS QSRWLVKSGE LTALEFSVSP GLRRKLTTRP
1410 1420 1430 1440 1450
VHLHLFNDCL LLSRPREGSR FLVFDHAPFS SIRGEKCEMK LHGPHKNLFR
1460 1470 1480 1490 1500
LFLLHNAQGT QVEFLFRTET QSEKLRWISA LAMPREELDL LECYDSPQVQ
1510 1520 1530 1540 1550
CLRAYKPREN DELALEKADV VMVTQQSSDG WLEGVRLSDG EQGWFPVQQV
1560 1570 1580
EFISNPEVRA QNLKEAHRVK TAKLQLVEQQ V
Length:1,581
Mass (Da):176,664
Last modified:April 5, 2011 - v1
Checksum:iCE61F8807C1C4102
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R1F1S4R1F1_MOUSE
Rho guanine nucleotide exchange fac...
Arhgef5
796Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC153885 Genomic DNA No translation available.
AC158671 Genomic DNA No translation available.
BC006829 mRNA Translation: AAH06829.1
BC025127 mRNA Translation: AAH25127.1
CCDSiCCDS51759.1
RefSeqiNP_598435.1, NM_133674.1
XP_006506450.1, XM_006506387.2
UniGeneiMm.320978

Genome annotation databases

EnsembliENSMUST00000031750; ENSMUSP00000031750; ENSMUSG00000033542
GeneIDi54324
KEGGimmu:54324
UCSCiuc009bsn.1 mouse
uc009bso.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC153885 Genomic DNA No translation available.
AC158671 Genomic DNA No translation available.
BC006829 mRNA Translation: AAH06829.1
BC025127 mRNA Translation: AAH25127.1
CCDSiCCDS51759.1
RefSeqiNP_598435.1, NM_133674.1
XP_006506450.1, XM_006506387.2
UniGeneiMm.320978

3D structure databases

SMRiE9Q7D5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031750

PTM databases

iPTMnetiE9Q7D5
PhosphoSitePlusiE9Q7D5

Proteomic databases

MaxQBiE9Q7D5
PaxDbiE9Q7D5
PeptideAtlasiE9Q7D5
PRIDEiE9Q7D5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031750; ENSMUSP00000031750; ENSMUSG00000033542
GeneIDi54324
KEGGimmu:54324
UCSCiuc009bsn.1 mouse
uc009bso.2 mouse

Organism-specific databases

CTDi7984
MGIiMGI:1858952 Arhgef5

Phylogenomic databases

eggNOGiKOG3523 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00550000074493
HOGENOMiHOG000007536
HOVERGENiHBG058673
InParanoidiE9Q7D5
KOiK20684
OMAiWPHRRDS
OrthoDBiEOG091G00P4
TreeFamiTF342609

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Miscellaneous databases

PROiPR:E9Q7D5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033542 Expressed in 225 organ(s), highest expression level in placenta labyrinth
ExpressionAtlasiE9Q7D5 baseline and differential
GenevisibleiE9Q7D5 MM

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR029212 ARHGEF5/35_N
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF15441 ARHGEF5_35, 1 hit
PF00621 RhoGEF, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARHG5_MOUSE
AccessioniPrimary (citable) accession number: E9Q7D5
Secondary accession number(s): Q8R172, Q922T1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: April 5, 2011
Last modified: September 12, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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