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Protein

Ubiquitin conjugation factor E4 A

Gene

Ube4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-ubiquitin ligase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 ACurated (EC:2.3.2.-1 Publication)
Gene namesi
Name:Ube4aImported
Synonyms:Ufd2bImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2154580 Ube4a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004307911 – 1028Ubiquitin conjugation factor E4 AAdd BLAST1028

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiE9Q735
PaxDbiE9Q735
PeptideAtlasiE9Q735
PRIDEiE9Q735

PTM databases

PhosphoSitePlusiE9Q735

Expressioni

Tissue specificityi

Expressed in liver, heart, brain, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000059890 Expressed in 215 organ(s), highest expression level in trigeminal ganglion
ExpressionAtlasiE9Q735 baseline and differential
GenevisibleiE9Q735 MM

Interactioni

Protein-protein interaction databases

BioGridi228292, 4 interactors
STRINGi10090.ENSMUSP00000113346

Structurei

3D structure databases

ProteinModelPortaliE9Q735
SMRiE9Q735
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini949 – 1023U-boxPROSITE-ProRule annotationAdd BLAST75

Domaini

The U-box domain is required for the ubiquitin protein ligase activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2042 Eukaryota
COG5113 LUCA
GeneTreeiENSGT00390000009300
KOiK10596
TreeFamiTF300802

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR019474 Ub_conjug_fac_E4_core
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
PF10408 Ufd2P_core, 2 hits
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

E9Q735-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTDQENNNNI SSNPFAALFG SLADAKQFAA IQKEQLKQQS DELPASPDDS
60 70 80 90 100
DNSVSESLDE FDYSVSEISR SFRTHQEMCE QLNINHMIQR IFLITLDNSD
110 120 130 140 150
PSLKSGNGIP SRCVYLEEMA VELEDQDWLD MSNVEQAIFA RLLLQDPGNH
160 170 180 190 200
LISMTSSTTL NLSADRDAGE RHIFCYLYSC FQRAKEEITK VPENLLPFAV
210 220 230 240 250
QCRNLTVSNT RTVLLTPEIY VDQNIHEQLV DLMLEAIQGA HFEDVTEFLE
260 270 280 290 300
EVIEALLLDE EVRTFPEVMI PVFDILLSRI KDLELCQILL YAYLDILLYF
310 320 330 340 350
TRQKDMAKVF LEYIQPKDPS NGQMYQKTLL GVILNISCLL KTPGVVENHG
360 370 380 390 400
FFLNPSRSSP QEIKVQEANI HQFMAQFHEK IYQMLKNLLQ LSPETKHCIL
410 420 430 440 450
FWLGNCLHAN AGRTKIWANQ MPEIFFQMYA SDAFFLNLGA ALLKLCQPFC
460 470 480 490 500
KPRSSRLLTF NPTYCVLKDL NDEERKIKSV HMRGLDKETC LIPAVQEPTF
510 520 530 540 550
PQSYNLVTEN LALTEYTLYL GFHRLHDQMV KINQNLHRLQ VAWRDAQQSS
560 570 580 590 600
SPAADNLREQ FERLMTIYLS TKTAMTEPQM LQNCLNLQVS MAVLLVQLAI
610 620 630 640 650
GNEGSQPIEL SFPLPDGYSS LAYVPEFFAD NLGDFLIFLR RFAEDILETS
660 670 680 690 700
ADSLEHVLHF ITIFTGSIER MKNPHLRAKL AEVLEAVMPH LDQTPSPLVS
710 720 730 740 750
SVFHRKRVFC NFPYAPQLAE ALIKVFVDIE FTGDPHQFEQ KFNYRRPMYP
760 770 780 790 800
ILRYMWGTDC YRESIKYLSK IKIQQIEKDR GEWESLTPEA RREKEAGLQM
810 820 830 840 850
FGQLARFHNI MSNETIGTLS FLTSEIKSLF VHPFLAERII SMLNYFLQHL
860 870 880 890 900
VGPKMGALKV KDFSEFDFKP QQLVSDICTI YLNLGDEENF CATVPKDGRS
910 920 930 940 950
YSPTLFAQTV RVLKKINKPG NMIVAFSNLA ERIKSLADLQ QQEEETYADA
960 970 980 990 1000
CDEFLDPIMS TLMSDPVVLP SSRVTVDRST IARHLLSDQT DPFNRSPLTM
1010 1020
DQIRPNTELK EKIQRWLAER KQQKEQPE
Length:1,028
Mass (Da):118,198
Last modified:April 5, 2011 - v1
Checksum:i00221784ECDD8761
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3X9Y5G3X9Y5_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a mCG_50277
1,085Annotation score:
E9Q442E9Q442_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
547Annotation score:
D3YXR7D3YXR7_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
96Annotation score:
D6RGN7D6RGN7_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
84Annotation score:
Q8VDS1Q8VDS1_MOUSE
Ube4a protein
Ube4a
406Annotation score:
A0A1L1SV76A0A1L1SV76_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
150Annotation score:
A0A1L1SUX2A0A1L1SUX2_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
206Annotation score:
A0A1L1SRI7A0A1L1SRI7_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
197Annotation score:
A0A1L1SV29A0A1L1SV29_MOUSE
Ubiquitin conjugation factor E4 A
Ube4a
51Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC061963 Genomic DNA No translation available.
CCDSiCCDS23122.2
RefSeqiNP_663375.3, NM_145400.3
UniGeneiMm.215069
Mm.421524

Genome annotation databases

EnsembliENSMUST00000117506; ENSMUSP00000113346; ENSMUSG00000059890
GeneIDi140630
KEGGimmu:140630
UCSCiuc009peu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC061963 Genomic DNA No translation available.
CCDSiCCDS23122.2
RefSeqiNP_663375.3, NM_145400.3
UniGeneiMm.215069
Mm.421524

3D structure databases

ProteinModelPortaliE9Q735
SMRiE9Q735
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228292, 4 interactors
STRINGi10090.ENSMUSP00000113346

PTM databases

PhosphoSitePlusiE9Q735

Proteomic databases

MaxQBiE9Q735
PaxDbiE9Q735
PeptideAtlasiE9Q735
PRIDEiE9Q735

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117506; ENSMUSP00000113346; ENSMUSG00000059890
GeneIDi140630
KEGGimmu:140630
UCSCiuc009peu.2 mouse

Organism-specific databases

CTDi9354
MGIiMGI:2154580 Ube4a

Phylogenomic databases

eggNOGiKOG2042 Eukaryota
COG5113 LUCA
GeneTreeiENSGT00390000009300
KOiK10596
TreeFamiTF300802

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiUbe4a mouse
PROiPR:E9Q735
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059890 Expressed in 215 organ(s), highest expression level in trigeminal ganglion
ExpressionAtlasiE9Q735 baseline and differential
GenevisibleiE9Q735 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR019474 Ub_conjug_fac_E4_core
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
PF10408 Ufd2P_core, 2 hits
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE4A_MOUSE
AccessioniPrimary (citable) accession number: E9Q735
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: April 5, 2011
Last modified: September 12, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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