UniProtKB - E9Q5G3 (KIF23_MOUSE)
Protein
Kinesin-like protein KIF23
Gene
Kif23
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 112 – 119 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- microtubule binding Source: MGI
- microtubule motor activity Source: GO_Central
GO - Biological processi
- microtubule-based movement Source: GO_Central
- mitotic cytokinesis Source: UniProtKB
- mitotic spindle midzone assembly Source: MGI
- positive regulation of cytokinesis Source: MGI
Keywordsi
Molecular function | Motor protein |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-68884, Mitotic Telophase/Cytokinesis R-MMU-983189, Kinesins |
Names & Taxonomyi
Protein namesi | Recommended name: Kinesin-like protein KIF23 |
Gene namesi | Name:Kif23 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1919069, Kif23 |
Subcellular locationi
Cytoskeleton
- spindle By similarity
Nucleus
- Nucleus By similarity
Other locations
- Midbody ring 1 Publication
Note: Localizes to the interzone of mitotic spindles (By similarity). Detected at the midbody during later stages of mitotic cytokinesis.By similarity
Cytoskeleton
- centralspindlin complex Source: MGI
- centrosome Source: MGI
- kinesin complex Source: GO_Central
- microtubule Source: GO_Central
- mitotic spindle Source: MGI
- spindle Source: MGI
Extracellular region or secreted
- intercellular bridge Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB-KW
- Flemming body Source: MGI
- midbody Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425085 | 1 – 953 | Kinesin-like protein KIF23Add BLAST | 953 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 155 | PhosphoserineBy similarity | 1 | |
Modified residuei | 160 | PhosphoserineCombined sources | 1 | |
Cross-linki | 572 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 587 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 606 | PhosphoserineBy similarity | 1 | |
Cross-linki | 625 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 648 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 663 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 666 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 683 | PhosphoserineCombined sources | 1 | |
Modified residuei | 685 | PhosphoserineCombined sources | 1 | |
Modified residuei | 739 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 807 | PhosphoserineBy similarity | 1 | |
Cross-linki | 816 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 847 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 860 | PhosphoserineCombined sources | 1 | |
Cross-linki | 867 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 870 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 892 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 904 | PhosphoserineBy similarity | 1 | |
Modified residuei | 920 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 949 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Ubiquitinated. Deubiquitinated by USP8/UBPY (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | E9Q5G3 |
jPOSTi | E9Q5G3 |
MaxQBi | E9Q5G3 |
PaxDbi | E9Q5G3 |
PeptideAtlasi | E9Q5G3 |
PRIDEi | E9Q5G3 |
PTM databases
iPTMneti | E9Q5G3 |
PhosphoSitePlusi | E9Q5G3 |
Expressioni
Tissue specificityi
Detected in testis and ovary from newborn mice (at protein level). Detected in brain, spinal cord and small intestine.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000032254, Expressed in secondary oocyte and 245 other tissues |
ExpressionAtlasi | E9Q5G3, baseline and differential |
Genevisiblei | E9Q5G3, MM |
Interactioni
Subunit structurei
Heterotetramer of two molecules each of RACGAP1 and KIF23.
Found in the centralspindlin complex.
Interacts with RACGAP1; the interaction is direct.
Interacts with ECT2 and PRC1.
Interacts with ANXA11 during cytokinesis.
Interacts with BIRC6/bruce and USP8/UBPY (By similarity).
Interacts with ARF6, forming heterodimers and heterotetramers.
By similarity1 PublicationGO - Molecular functioni
- microtubule binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 214951, 31 interactors |
IntActi | E9Q5G3, 15 interactors |
STRINGi | 10090.ENSMUSP00000034815 |
Miscellaneous databases
RNActi | E9Q5G3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 436 | Kinesin motorPROSITE-ProRule annotationAdd BLAST | 412 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 542 – 618 | Sequence analysisAdd BLAST | 77 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 7 – 11 | Nuclear localization signalSequence analysis | 5 |
Sequence similaritiesi
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0247, Eukaryota |
GeneTreei | ENSGT00940000155837 |
HOGENOMi | CLU_001485_13_0_1 |
InParanoidi | E9Q5G3 |
OrthoDBi | 314538at2759 |
TreeFami | TF105232 |
Family and domain databases
Gene3Di | 3.40.50.12000, 1 hit 3.40.850.10, 2 hits |
InterProi | View protein in InterPro IPR032384, Kif23_Arf-bd IPR038105, Kif23_Arf-bd_sf IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR24115, PTHR24115, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit PF16540, MKLP1_Arf_bdg, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
E9Q5G3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSAKAKTVR KPVIKKGSQT NLKDPVGVYC RVRPLSFPDQ ECCVEVINST
60 70 80 90 100
TLQLHTPEGY RLNRNGDYKE TQYSFKRVFG THTTQKELFD VVANPLVDDL
110 120 130 140 150
IHGKNGLLFT YGVTGSGKTY TMTGSPGSGG LLPRCLNMIF NSIGSFQAKR
160 170 180 190 200
YVFKSNDRNS MEIQCEVDAL LERQKREALP IPKTPSSKRQ ADPEFADMIN
210 220 230 240 250
VQEFCKAEEV DEDSVYGVFV SYIEIYNNYI YDLLEEVQFD PIKPKLPQSK
260 270 280 290 300
TLREDKNHNM YVAGCTEVEV KSTEEAFEVF WRGQKKRRIA NTHLNRESSR
310 320 330 340 350
SHSVFSIKLV QAPLDADGDN VLQEKEQITI SQLSLVDLAG SERTNRTKAE
360 370 380 390 400
GNRLREAGNI NQSLMTLRTC MEVLRENQTY GTNKMVPYRD SKLTHLFKNY
410 420 430 440 450
FDGEGKVRMI VCVNPKAEDY EESLQVMRFA EVTQEVEVAR PVDKVICGLT
460 470 480 490 500
PGRRYRNLPR GGPVGDEPLV PEVILQSFPP LPPCKLLDIN DEETLPKLAD
510 520 530 540 550
TLEKRHHLRQ LMTEDLNKKC LAFKALLKEF DNSLSNKENY VQEKLNEREK
560 570 580 590 600
VISGQKLEIE RLEKKNKTLE YKIEILEKTT TIYEEDKRNL QQELESQNQK
610 620 630 640 650
LQRQFSDKRR LEARLQGMVT ETSMKWQKEC ERRVAATQLE MQNKLWVKDE
660 670 680 690 700
KLKQLKAIVT EPKPEKPERP SRERDREKII PRSVSPSPLP LSSNNIAQIS
710 720 730 740 750
NGQQLMSQPQ LHRRSNSCSS ISVASCISEW EQKLSPFSTP VNVTSLARHR
760 770 780 790 800
QQEPGQSKTC IVSDRRRGMC WTEGREMVPT FSSEIGVEED HCRRNTPIPV
810 820 830 840 850
RHRRSRSAGS RWVDHKPASN VQTETVMQPH VPHAITVSVA NEKALAKCEK
860 870 880 890 900
YMLTHQELAS DGEIQTKVIK GDVYKTRGGG QSVQFTDIET LKQELPTGSR
910 920 930 940 950
KRRSSTLAPA QPDGTESEWT DVETRCSVAV EMRAGSQLGP GYQHHAQPKR
KKP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SRP4 | A0A1L1SRP4_MOUSE | Kinesin-like protein | Kif23 | 563 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 464 | V → I in AAH47273 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC151969 Genomic DNA No translation available. BC047273 mRNA Translation: AAH47273.1 AB054027 Genomic DNA Translation: BAB32491.1 |
CCDSi | CCDS40662.1 |
RefSeqi | NP_077207.3, NM_024245.4 |
Genome annotation databases
Ensembli | ENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254 |
GeneIDi | 71819 |
KEGGi | mmu:71819 |
UCSCi | uc009pzu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC151969 Genomic DNA No translation available. BC047273 mRNA Translation: AAH47273.1 AB054027 Genomic DNA Translation: BAB32491.1 |
CCDSi | CCDS40662.1 |
RefSeqi | NP_077207.3, NM_024245.4 |
3D structure databases
SMRi | E9Q5G3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214951, 31 interactors |
IntActi | E9Q5G3, 15 interactors |
STRINGi | 10090.ENSMUSP00000034815 |
PTM databases
iPTMneti | E9Q5G3 |
PhosphoSitePlusi | E9Q5G3 |
Proteomic databases
EPDi | E9Q5G3 |
jPOSTi | E9Q5G3 |
MaxQBi | E9Q5G3 |
PaxDbi | E9Q5G3 |
PeptideAtlasi | E9Q5G3 |
PRIDEi | E9Q5G3 |
Protocols and materials databases
Antibodypediai | 4171, 181 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254 |
GeneIDi | 71819 |
KEGGi | mmu:71819 |
UCSCi | uc009pzu.2, mouse |
Organism-specific databases
CTDi | 9493 |
MGIi | MGI:1919069, Kif23 |
Phylogenomic databases
eggNOGi | KOG0247, Eukaryota |
GeneTreei | ENSGT00940000155837 |
HOGENOMi | CLU_001485_13_0_1 |
InParanoidi | E9Q5G3 |
OrthoDBi | 314538at2759 |
TreeFami | TF105232 |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-68884, Mitotic Telophase/Cytokinesis R-MMU-983189, Kinesins |
Miscellaneous databases
BioGRID-ORCSi | 71819, 6 hits in 18 CRISPR screens |
ChiTaRSi | Kif23, mouse |
PROi | PR:E9Q5G3 |
RNActi | E9Q5G3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032254, Expressed in secondary oocyte and 245 other tissues |
ExpressionAtlasi | E9Q5G3, baseline and differential |
Genevisiblei | E9Q5G3, MM |
Family and domain databases
Gene3Di | 3.40.50.12000, 1 hit 3.40.850.10, 2 hits |
InterProi | View protein in InterPro IPR032384, Kif23_Arf-bd IPR038105, Kif23_Arf-bd_sf IPR027640, Kinesin-like_fam IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase |
PANTHERi | PTHR24115, PTHR24115, 1 hit |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit PF16540, MKLP1_Arf_bdg, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KIF23_MOUSE | |
Accessioni | E9Q5G3Primary (citable) accession number: E9Q5G3 Secondary accession number(s): Q80V30, Q99PT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 22, 2014 |
Last sequence update: | April 5, 2011 | |
Last modified: | February 10, 2021 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families