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Protein

Kinesin-like protein KIF23

Gene

Kif23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi112 – 119ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • microtubule binding Source: MGI
  • microtubule motor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionMotor protein
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68884 Mitotic Telophase/Cytokinesis
R-MMU-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF23
Gene namesi
Name:Kif23
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1919069 Kif23

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004250851 – 953Kinesin-like protein KIF23Add BLAST953

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155PhosphoserineBy similarity1
Modified residuei160PhosphoserineCombined sources1
Cross-linki572Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki587Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei606PhosphoserineBy similarity1
Cross-linki625Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki648Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki663Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki666Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei683PhosphoserineCombined sources1
Modified residuei685PhosphoserineCombined sources1
Modified residuei739PhosphothreonineBy similarity1
Modified residuei807PhosphoserineBy similarity1
Cross-linki816Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki847Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei860PhosphoserineCombined sources1
Cross-linki867Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki870Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki892Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei904PhosphoserineBy similarity1
Modified residuei920PhosphothreonineCombined sources1
Cross-linki949Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

Ubiquitinated. Deubiquitinated by USP8/UBPY (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiE9Q5G3
MaxQBiE9Q5G3
PaxDbiE9Q5G3
PeptideAtlasiE9Q5G3
PRIDEiE9Q5G3

PTM databases

iPTMnetiE9Q5G3
PhosphoSitePlusiE9Q5G3

Expressioni

Tissue specificityi

Detected in testis and ovary from newborn mice (at protein level). Detected in brain, spinal cord and small intestine.2 Publications

Gene expression databases

BgeeiENSMUSG00000032254 Expressed in 225 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiE9Q5G3 baseline and differential
GenevisibleiE9Q5G3 MM

Interactioni

Subunit structurei

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY (By similarity). Interacts with ARF6, forming heterodimers and heterotetramers.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214951, 14 interactors
IntActiE9Q5G3, 15 interactors
STRINGi10090.ENSMUSP00000034815

Structurei

3D structure databases

ProteinModelPortaliE9Q5G3
SMRiE9Q5G3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 436Kinesin motorPROSITE-ProRule annotationAdd BLAST412

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili542 – 618Sequence analysisAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 11Nuclear localization signalSequence analysis5

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0247 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000273893
HOVERGENiHBG052253
InParanoidiE9Q5G3
KOiK17387
OrthoDBiEOG091G024C
TreeFamiTF105232

Family and domain databases

Gene3Di3.40.50.12000, 1 hit
3.40.850.10, 2 hits
InterProiView protein in InterPro
IPR032924 KIF23
IPR032384 Kif23_Arf-bd
IPR038105 Kif23_Arf-bd_sf
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PTHR24115:SF467 PTHR24115:SF467, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PF16540 MKLP1_Arf_bdg, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

E9Q5G3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSAKAKTVR KPVIKKGSQT NLKDPVGVYC RVRPLSFPDQ ECCVEVINST
60 70 80 90 100
TLQLHTPEGY RLNRNGDYKE TQYSFKRVFG THTTQKELFD VVANPLVDDL
110 120 130 140 150
IHGKNGLLFT YGVTGSGKTY TMTGSPGSGG LLPRCLNMIF NSIGSFQAKR
160 170 180 190 200
YVFKSNDRNS MEIQCEVDAL LERQKREALP IPKTPSSKRQ ADPEFADMIN
210 220 230 240 250
VQEFCKAEEV DEDSVYGVFV SYIEIYNNYI YDLLEEVQFD PIKPKLPQSK
260 270 280 290 300
TLREDKNHNM YVAGCTEVEV KSTEEAFEVF WRGQKKRRIA NTHLNRESSR
310 320 330 340 350
SHSVFSIKLV QAPLDADGDN VLQEKEQITI SQLSLVDLAG SERTNRTKAE
360 370 380 390 400
GNRLREAGNI NQSLMTLRTC MEVLRENQTY GTNKMVPYRD SKLTHLFKNY
410 420 430 440 450
FDGEGKVRMI VCVNPKAEDY EESLQVMRFA EVTQEVEVAR PVDKVICGLT
460 470 480 490 500
PGRRYRNLPR GGPVGDEPLV PEVILQSFPP LPPCKLLDIN DEETLPKLAD
510 520 530 540 550
TLEKRHHLRQ LMTEDLNKKC LAFKALLKEF DNSLSNKENY VQEKLNEREK
560 570 580 590 600
VISGQKLEIE RLEKKNKTLE YKIEILEKTT TIYEEDKRNL QQELESQNQK
610 620 630 640 650
LQRQFSDKRR LEARLQGMVT ETSMKWQKEC ERRVAATQLE MQNKLWVKDE
660 670 680 690 700
KLKQLKAIVT EPKPEKPERP SRERDREKII PRSVSPSPLP LSSNNIAQIS
710 720 730 740 750
NGQQLMSQPQ LHRRSNSCSS ISVASCISEW EQKLSPFSTP VNVTSLARHR
760 770 780 790 800
QQEPGQSKTC IVSDRRRGMC WTEGREMVPT FSSEIGVEED HCRRNTPIPV
810 820 830 840 850
RHRRSRSAGS RWVDHKPASN VQTETVMQPH VPHAITVSVA NEKALAKCEK
860 870 880 890 900
YMLTHQELAS DGEIQTKVIK GDVYKTRGGG QSVQFTDIET LKQELPTGSR
910 920 930 940 950
KRRSSTLAPA QPDGTESEWT DVETRCSVAV EMRAGSQLGP GYQHHAQPKR

KKP
Length:953
Mass (Da):108,776
Last modified:April 5, 2011 - v1
Checksum:iDE6E050D59A18513
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1L1SRP4A0A1L1SRP4_MOUSE
Kinesin-like protein
Kif23
563Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti464V → I in AAH47273 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC151969 Genomic DNA No translation available.
BC047273 mRNA Translation: AAH47273.1
AB054027 Genomic DNA Translation: BAB32491.1
CCDSiCCDS40662.1
RefSeqiNP_077207.3, NM_024245.4
UniGeneiMm.259374

Genome annotation databases

EnsembliENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254
GeneIDi71819
KEGGimmu:71819
UCSCiuc009pzu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC151969 Genomic DNA No translation available.
BC047273 mRNA Translation: AAH47273.1
AB054027 Genomic DNA Translation: BAB32491.1
CCDSiCCDS40662.1
RefSeqiNP_077207.3, NM_024245.4
UniGeneiMm.259374

3D structure databases

ProteinModelPortaliE9Q5G3
SMRiE9Q5G3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214951, 14 interactors
IntActiE9Q5G3, 15 interactors
STRINGi10090.ENSMUSP00000034815

PTM databases

iPTMnetiE9Q5G3
PhosphoSitePlusiE9Q5G3

Proteomic databases

EPDiE9Q5G3
MaxQBiE9Q5G3
PaxDbiE9Q5G3
PeptideAtlasiE9Q5G3
PRIDEiE9Q5G3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254
GeneIDi71819
KEGGimmu:71819
UCSCiuc009pzu.2 mouse

Organism-specific databases

CTDi9493
MGIiMGI:1919069 Kif23

Phylogenomic databases

eggNOGiKOG0247 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000273893
HOVERGENiHBG052253
InParanoidiE9Q5G3
KOiK17387
OrthoDBiEOG091G024C
TreeFamiTF105232

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68884 Mitotic Telophase/Cytokinesis
R-MMU-983189 Kinesins

Miscellaneous databases

ChiTaRSiKif23 mouse
PROiPR:E9Q5G3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032254 Expressed in 225 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiE9Q5G3 baseline and differential
GenevisibleiE9Q5G3 MM

Family and domain databases

Gene3Di3.40.50.12000, 1 hit
3.40.850.10, 2 hits
InterProiView protein in InterPro
IPR032924 KIF23
IPR032384 Kif23_Arf-bd
IPR038105 Kif23_Arf-bd_sf
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PTHR24115:SF467 PTHR24115:SF467, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PF16540 MKLP1_Arf_bdg, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKIF23_MOUSE
AccessioniPrimary (citable) accession number: E9Q5G3
Secondary accession number(s): Q80V30, Q99PT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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