Kif23

Functionⁱ

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro (By similarity).By similarity

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	112 – 119	ATPPROSITE-ProRule annotation		8

GO - Molecular functionⁱ

ATPase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
ATP binding Source: UniProtKB-KW
microtubule binding Source: MGI
microtubule motor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actomyosin contractile ring assembly Source: InterPro
microtubule-based movement
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
mitotic cytokinesis Source: UniProtKB
mitotic spindle midzone assembly Source: MGI
plus-end-directed vesicle transport along microtubule Source: InterPro
positive regulation of cytokinesis Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Motor protein
Biological process	Cell cycle, Cell division, Mitosis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2132295 MHC class II antigen presentation R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-MMU-68884 Mitotic Telophase/Cytokinesis R-MMU-983189 Kinesins

Reactomeⁱ

R-MMU-2132295 MHC class II antigen presentation
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68884 Mitotic Telophase/Cytokinesis
R-MMU-983189 Kinesins

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Kinesin-like protein KIF23
Gene namesⁱ	Name:Kif23
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 9

Organism-specific databases

MGIⁱ	MGI:1919069 Kif23

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000425085	1 – 953	Kinesin-like protein KIF23Add BLAST		953

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	155	PhosphoserineBy similarity	1
Modified residueⁱ	160	PhosphoserineCombined sources	1
Cross-linkⁱ	572	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	587	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	606	PhosphoserineBy similarity	1
Cross-linkⁱ	625	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	648	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	663	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	666	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	683	PhosphoserineCombined sources	1
Modified residueⁱ	685	PhosphoserineCombined sources	1
Modified residueⁱ	739	PhosphothreonineBy similarity	1
Modified residueⁱ	807	PhosphoserineBy similarity	1
Cross-linkⁱ	816	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	847	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	860	PhosphoserineCombined sources	1
Cross-linkⁱ	867	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	870	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	892	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	904	PhosphoserineBy similarity	1
Modified residueⁱ	920	PhosphothreonineCombined sources	1
Cross-linkⁱ	949	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationⁱ

Ubiquitinated. Deubiquitinated by USP8/UBPY (By similarity).By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	E9Q5G3
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	E9Q5G3
PaxDbⁱ	E9Q5G3
PeptideAtlas More...PeptideAtlasⁱ	E9Q5G3
PRIDEⁱ	E9Q5G3

PTM databases

iPTMnetⁱ	E9Q5G3
PhosphoSitePlusⁱ	E9Q5G3

Expressionⁱ

Tissue specificityⁱ

Detected in testis and ovary from newborn mice (at protein level). Detected in brain, spinal cord and small intestine.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000032254 Expressed in 225 organ(s), highest expression level in secondary oocyte
ExpressionAtlasⁱ	E9Q5G3 baseline and differential
Genevisibleⁱ	E9Q5G3 MM

Interactionⁱ

Subunit structureⁱ

Heterotetramer of two molecules each of RACGAP1 and KIF23. Found in the centralspindlin complex composed of RACGAP1 and KIF23. Interacts with RACGAP1; the interaction is direct. Interacts with ECT2 and PRC1. Interacts with ANXA11 during cytokinesis. Interacts with BIRC6/bruce and USP8/UBPY (By similarity). Interacts with ARF6, forming heterodimers and heterotetramers.By similarity1 Publication

GO - Molecular functionⁱ

microtubule binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	214951, 14 interactors
IntActⁱ	E9Q5G3, 15 interactors
STRINGⁱ	10090.ENSMUSP00000034815

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	E9Q5G3
SMRⁱ	E9Q5G3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	25 – 436	Kinesin motorPROSITE-ProRule annotationAdd BLAST		412

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	542 – 618	Sequence analysisAdd BLAST		77

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	7 – 11	Nuclear localization signalSequence analysis		5

Sequence similaritiesⁱ

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG0247 Eukaryota COG5059 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150825
HOGENOMⁱ	HOG000273893
HOVERGENⁱ	HBG052253
InParanoidⁱ	E9Q5G3
KEGG Orthology (KO) More...KOⁱ	K17387
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G024C
TreeFamⁱ	TF105232

Family and domain databases

Gene3Dⁱ	3.40.50.12000, 1 hit 3.40.850.10, 2 hits
InterProⁱ	View protein in InterPro IPR032924 KIF23 IPR032384 Kif23_Arf-bd IPR038105 Kif23_Arf-bd_sf IPR027640 Kinesin-like_fam IPR019821 Kinesin_motor_CS IPR001752 Kinesin_motor_dom IPR036961 Kinesin_motor_dom_sf IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR24115 PTHR24115, 1 hit PTHR24115:SF467 PTHR24115:SF467, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00225 Kinesin, 1 hit PF16540 MKLP1_Arf_bdg, 1 hit
PRINTSⁱ	PR00380 KINESINHEAVY
SMARTⁱ	View protein in SMART SM00129 KISc, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00411 KINESIN_MOTOR_1, 1 hit PS50067 KINESIN_MOTOR_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

E9Q5G3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKSAKAKTVR KPVIKKGSQT NLKDPVGVYC RVRPLSFPDQ ECCVEVINST 
        60         70         80         90        100
TLQLHTPEGY RLNRNGDYKE TQYSFKRVFG THTTQKELFD VVANPLVDDL 
       110        120        130        140        150
IHGKNGLLFT YGVTGSGKTY TMTGSPGSGG LLPRCLNMIF NSIGSFQAKR 
       160        170        180        190        200
YVFKSNDRNS MEIQCEVDAL LERQKREALP IPKTPSSKRQ ADPEFADMIN 
       210        220        230        240        250
VQEFCKAEEV DEDSVYGVFV SYIEIYNNYI YDLLEEVQFD PIKPKLPQSK 
       260        270        280        290        300
TLREDKNHNM YVAGCTEVEV KSTEEAFEVF WRGQKKRRIA NTHLNRESSR 
       310        320        330        340        350
SHSVFSIKLV QAPLDADGDN VLQEKEQITI SQLSLVDLAG SERTNRTKAE 
       360        370        380        390        400
GNRLREAGNI NQSLMTLRTC MEVLRENQTY GTNKMVPYRD SKLTHLFKNY 
       410        420        430        440        450
FDGEGKVRMI VCVNPKAEDY EESLQVMRFA EVTQEVEVAR PVDKVICGLT 
       460        470        480        490        500
PGRRYRNLPR GGPVGDEPLV PEVILQSFPP LPPCKLLDIN DEETLPKLAD 
       510        520        530        540        550
TLEKRHHLRQ LMTEDLNKKC LAFKALLKEF DNSLSNKENY VQEKLNEREK 
       560        570        580        590        600
VISGQKLEIE RLEKKNKTLE YKIEILEKTT TIYEEDKRNL QQELESQNQK 
       610        620        630        640        650
LQRQFSDKRR LEARLQGMVT ETSMKWQKEC ERRVAATQLE MQNKLWVKDE 
       660        670        680        690        700
KLKQLKAIVT EPKPEKPERP SRERDREKII PRSVSPSPLP LSSNNIAQIS 
       710        720        730        740        750
NGQQLMSQPQ LHRRSNSCSS ISVASCISEW EQKLSPFSTP VNVTSLARHR 
       760        770        780        790        800
QQEPGQSKTC IVSDRRRGMC WTEGREMVPT FSSEIGVEED HCRRNTPIPV 
       810        820        830        840        850
RHRRSRSAGS RWVDHKPASN VQTETVMQPH VPHAITVSVA NEKALAKCEK 
       860        870        880        890        900
YMLTHQELAS DGEIQTKVIK GDVYKTRGGG QSVQFTDIET LKQELPTGSR 
       910        920        930        940        950
KRRSSTLAPA QPDGTESEWT DVETRCSVAV EMRAGSQLGP GYQHHAQPKR 

KKP

Length:953

Mass (Da):108,776

Last modified:April 5, 2011 - v1

Checksum:ⁱDE6E050D59A18513

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1L1SRP4	A0A1L1SRP4_MOUSE		Kinesin-like protein Kinesin-like protein	Kif23	563	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	464	V → I in AAH47273 (PubMed:15489334).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC151969 Genomic DNA No translation available. BC047273 mRNA Translation: AAH47273.1 AB054027 Genomic DNA Translation: BAB32491.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS40662.1
NCBI Reference Sequences More...RefSeqⁱ	NP_077207.3, NM_024245.4
UniGeneⁱ	Mm.259374

Genome annotation databases

Ensemblⁱ	ENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254
GeneIDⁱ	71819
KEGGⁱ	mmu:71819
UCSC genome browser More...UCSCⁱ	uc009pzu.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
E9Q5G3	Kinesin-like protein (Fragment)		NEOLE		999	UniRef90_E9Q5G3
Kinesin-like protein		CRIGR		967
Kinesin-like protein		RAT		944
Kinesin-like protein (Fragment)		MOUSE		563
UPI00077DA48D		PERMB		979
+14

Protein	Similar proteins		Species	Score	Length	Source
E9Q5G3	Kinesin-like protein (Fragment)		NEOLE		999	UniRef50_E9Q5G3
Kinesin-like protein		CRIGR		967
Kinesin-like protein		RAT		944
Kinesin-like protein (Fragment)		MOUSE		563
Kinesin-like protein		Cebus capucinus imitator		873
+73

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AC151969 Genomic DNA No translation available. BC047273 mRNA Translation: AAH47273.1 AB054027 Genomic DNA Translation: BAB32491.1
CCDSⁱ	CCDS40662.1
RefSeqⁱ	NP_077207.3, NM_024245.4
UniGeneⁱ	Mm.259374

3D structure databases

ProteinModelPortalⁱ	E9Q5G3
SMRⁱ	E9Q5G3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	214951, 14 interactors
IntActⁱ	E9Q5G3, 15 interactors
STRINGⁱ	10090.ENSMUSP00000034815

PTM databases

iPTMnetⁱ	E9Q5G3
PhosphoSitePlusⁱ	E9Q5G3

Proteomic databases

EPDⁱ	E9Q5G3
MaxQBⁱ	E9Q5G3
PaxDbⁱ	E9Q5G3
PeptideAtlasⁱ	E9Q5G3
PRIDEⁱ	E9Q5G3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034815; ENSMUSP00000034815; ENSMUSG00000032254
GeneIDⁱ	71819
KEGGⁱ	mmu:71819
UCSCⁱ	uc009pzu.2 mouse

Organism-specific databases

CTDⁱ	9493
MGIⁱ	MGI:1919069 Kif23

Phylogenomic databases

eggNOGⁱ	KOG0247 Eukaryota COG5059 LUCA
GeneTreeⁱ	ENSGT00930000150825
HOGENOMⁱ	HOG000273893
HOVERGENⁱ	HBG052253
InParanoidⁱ	E9Q5G3
KOⁱ	K17387
OrthoDBⁱ	EOG091G024C
TreeFamⁱ	TF105232

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2132295 MHC class II antigen presentation R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-MMU-68884 Mitotic Telophase/Cytokinesis R-MMU-983189 Kinesins

Reactomeⁱ

R-MMU-2132295 MHC class II antigen presentation
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-68884 Mitotic Telophase/Cytokinesis
R-MMU-983189 Kinesins

Miscellaneous databases

ChiTaRSⁱ	Kif23 mouse
Protein Ontology More...PROⁱ	PR:E9Q5G3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000032254 Expressed in 225 organ(s), highest expression level in secondary oocyte
ExpressionAtlasⁱ	E9Q5G3 baseline and differential
Genevisibleⁱ	E9Q5G3 MM

Family and domain databases

Gene3Dⁱ	3.40.50.12000, 1 hit 3.40.850.10, 2 hits
InterProⁱ	View protein in InterPro IPR032924 KIF23 IPR032384 Kif23_Arf-bd IPR038105 Kif23_Arf-bd_sf IPR027640 Kinesin-like_fam IPR019821 Kinesin_motor_CS IPR001752 Kinesin_motor_dom IPR036961 Kinesin_motor_dom_sf IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR24115 PTHR24115, 1 hit PTHR24115:SF467 PTHR24115:SF467, 1 hit
Pfamⁱ	View protein in Pfam PF00225 Kinesin, 1 hit PF16540 MKLP1_Arf_bdg, 1 hit
PRINTSⁱ	PR00380 KINESINHEAVY
SMARTⁱ	View protein in SMART SM00129 KISc, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00411 KINESIN_MOTOR_1, 1 hit PS50067 KINESIN_MOTOR_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KIF23_MOUSE
Accessionⁱ	E9Q5G3Primary (citable) accession number: E9Q5G3 Secondary accession number(s): Q80V30, Q99PT8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 22, 2014
	Last sequence update:	April 5, 2011
	Last modified:	November 7, 2018
	This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniRef

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Supporting data

UniProtKB - E9Q5G3 (KIF23_MOUSE)

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Kinesin-like protein KIF23

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytoskeleton

Nucleus

Other locations

Cytoskeleton

Extracellular region or secreted

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ