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Protein

Phosphatidylinositol 4-kinase alpha

Gene

Pi4ka

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.By similarity

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.By similarity

Activity regulationi

Activated by Triton X-100, insensitive to inhibition by adenosine and inhibited by wortmannin (By similarity). The PI4K complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns4P) synthesis. Interaction with TMEM150A regulates PtdIns4P synthesis (By similarity).By similarity

GO - Molecular functioni

  • 1-phosphatidylinositol 4-kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-kinase alpha (EC:2.7.1.67By similarity)
Short name:
PI4-kinase alpha
Short name:
PI4K-alpha
Short name:
PtdIns-4-kinase alpha
Gene namesi
Name:Pi4kaImported
Synonyms:Pik4, Pik4ca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2448506 Pi4ka

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Early embryonic lethality. Conditional knockout mice show a dramatic depletion of cellular phosphatidylinositol 4-phosphate (PtdIns4P).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004356321 – 2105Phosphatidylinositol 4-kinase alphaAdd BLAST2105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233PhosphoserineBy similarity1
Modified residuei259PhosphoserineCombined sources1
Modified residuei260PhosphoserineCombined sources1
Modified residuei262PhosphoserineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei265PhosphoserineBy similarity1
Modified residuei268PhosphoserineCombined sources1
Modified residuei432PhosphoserineBy similarity1
Modified residuei1439PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiE9Q3L2
PeptideAtlasiE9Q3L2
PRIDEiE9Q3L2

PTM databases

iPTMnetiE9Q3L2
PhosphoSitePlusiE9Q3L2

Expressioni

Gene expression databases

BgeeiENSMUSG00000041720 Expressed in 298 organ(s), highest expression level in bed nucleus of stria terminalis

Interactioni

Subunit structurei

Component of a phosphatidylinositol 4-kinase (PI4K) complex, composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and FAM126 (FAM126A or FAM126B). Interacts with TMEM150A; regulating recruitment to the plasma membrane.By similarity

Protein-protein interaction databases

IntActiE9Q3L2, 5 interactors
STRINGi10090.ENSMUSP00000036162

Structurei

3D structure databases

SMRiE9Q3L2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1533 – 1721PIK helicalPROSITE-ProRule annotationAdd BLAST189
Domaini1848 – 2105PI3K/PI4KPROSITE-ProRule annotationAdd BLAST258

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0902 Eukaryota
COG5032 LUCA
HOGENOMiHOG000007272
HOVERGENiHBG103255
InParanoidiE9Q3L2
KOiK00888
OrthoDBiEOG091G03W5
TreeFamiTF102041

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 1 hit
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00613 PI3Ka, 1 hit
SMARTiView protein in SMART
SM00145 PI3Ka, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51545 PIK_HELICAL, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

E9Q3L2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAGARGGG GGGGGGGGGG SGSSSGSSTS RGFYFNTVLS LARSLAVQRP
60 70 80 90 100
ASLEKVQKLL CMCPVDFHGI FQLDERRRDA VIALGIFLIE SDLQHKDCVV
110 120 130 140 150
PYLLRLLRGL PKVYWVEEST ARKGRGNLPV AESFSFCLVT LLSDVACRDP
160 170 180 190 200
SLRDEILEAI LQVLHVLLGM CQALEIQEKE YLCKYAIPCL IGISRSFGRY
210 220 230 240 250
SNSEESLLSK LFPKVSPHSL RIPEELEGVR RRSFNDFRSI LPSNLLTVCQ
260 270 280 290 300
EGTLKRKTSS VSSISQVSPE RGIPPPSSPG GSAFHYFEAS CLPDGTALEP
310 320 330 340 350
EYYFSTISSS FSISPLFNGI TYKEFCIPLE MLRELLNLVK KIVEEPVLKS
360 370 380 390 400
LDAIVAGVME ANPSADLYYT TFSDPLYLTM FKMLRDTLYY MKDLPTSFVK
410 420 430 440 450
EIHDFVLEQF NMSQGELQKI LHDADRIHSE MSPLKLRCQA NAACVDLMVW
460 470 480 490 500
AVKDEQGAEN LCIKLSEKLQ SKTSSKVIIA HLPLLICCLQ GLGRLCERFP
510 520 530 540 550
VVVHSVTPSL RDFLVIPSPV LVKLYKYHSQ YHTVAGSDIK ISVTNEHSES
560 570 580 590 600
TLNVLPGKKN QPSMYEQLRD IAIDNICRCL KAGLTVDPVI VEAFLASLSN
610 620 630 640 650
RLYISQESDK DAHLIPDHTI RALGHIAVAL RDTPKVMEPI LQILQQKFCQ
660 670 680 690 700
PPSPLDVLII DQLGCLVITG NQYIYQEVWN LFQQISVKAS SVVYSATKDY
710 720 730 740 750
KDHGYRHCSL AVINALANIA ANIQEEHLVD ELLMNLLELF VQLGLEGKRA
760 770 780 790 800
SERASEKGPA LKASSSAGNL GVLIPVIAVL TRRLPPIKEA KPRLQKLFRD
810 820 830 840 850
FWLYSVLMGF AVEGSGLWPE EWYEGVCEIA TKSPLLTFPS KEPLRSVLQY
860 870 880 890 900
NSAMKNDTVT PAELSELRST IINLLDPPPE VSALINKLDF AMSTYLLSVY
910 920 930 940 950
RLEYMRVLRS TDPDRFQVMF CYFEDKAIQK DKSGMMQCVI AVADKVFDAF
960 970 980 990 1000
LNMMAEKAKT KENEEELERH AQFLLVNFNH IHKRIRRVAD KYLSGLVDKF
1010 1020 1030 1040 1050
PHLLWSGTVL KTMLDILQTL SLSLSADIHK DQPYYDIPDA PYRITVPDTY
1060 1070 1080 1090 1100
EARESIVKDF AARCGMILQE AMKWAPTVTK SHLQEYLNKH QNWVSGLSQH
1110 1120 1130 1140 1150
TGLAMATESI LHFAGYNKQN TTLGATQLTE RPACVKKDYS NFMASLNLRN
1160 1170 1180 1190 1200
RYAGEVHGMI RFSGATGQMS DLNKMMVQDL ITALDHSHPQ HYTQAMFKLT
1210 1220 1230 1240 1250
AMLISSKDCD PQLLHHLCWG PLRMFNEHGM ETALACWEWL LAGKNGVEVP
1260 1270 1280 1290 1300
FMREMAGAWH MTVEQKFGLF SVETKEADPL AASEASQPRP CPPEVTPHYI
1310 1320 1330 1340 1350
WIDFLVQRFE IAKYCSSDQV EIFSSLLQRS MSLNIGGARG SMNRHVAAIG
1360 1370 1380 1390 1400
PRFKLLTLGL SLLHADVVPN ATIRNVLREK IYSTAFDYFS CPPKFPTQGE
1410 1420 1430 1440 1450
KRLREDISIM IKFWTAMFSD KKYLTASQLV PPDNQDTRSN LDITVGSRQQ
1460 1470 1480 1490 1500
ATQGWINTYP LSSGMSTISK KSGMSKKTNR GSQLHKYYMK RRTLLLSLLA
1510 1520 1530 1540 1550
TEIERLITWY NPLSAPELEL DQAGENSVAN WRSKYISLSE KQWKDNVNLA
1560 1570 1580 1590 1600
WSISPYLAVQ LPARFKNTEA IGNEVTRLVR LDPGAVSDVP EAIKFLVTWH
1610 1620 1630 1640 1650
TIDADAPELS HVLCWAPTDP PTGLSYFSSM YPPHPLTAQY GVKVLRSFPP
1660 1670 1680 1690 1700
DAILFYIPQI VQALRYDKMG YVREYILWAA AKSQLLAHQF IWNMKTNIYL
1710 1720 1730 1740 1750
DEEGHQKDPD IGDLLEQLVE EITGSLSGPA KDFYQREFDF FNKITNVSAI
1760 1770 1780 1790 1800
IKPYPKGDER KKACLSALSE VKVQPGCYLP SNPEAIVLDI DYKSGTPMQS
1810 1820 1830 1840 1850
AAKAPYLAKF KVKRCGVSEL EKEGLQCRSD AEDECFSQEA DGKKICWQAA
1860 1870 1880 1890 1900
IFKVGDDCRQ DMLALQIIDL FKNIFQLVGL DLFVFPYRVV ATAPGCGVIE
1910 1920 1930 1940 1950
CIPDCTSRDQ LGRQTDFGMY DYFTRQYGDE STLAFQQARY NFIRSMAAYS
1960 1970 1980 1990 2000
LLLFLLQIKD RHNGNIMLDK KGHIIHIDFG FMFESSPGGN LGWEPDIKLT
2010 2020 2030 2040 2050
DEMVMIMGGK MEATPFKWFM EMCVRGYLAV RPYMDAVVSL VTLMLDTGLP
2060 2070 2080 2090 2100
CFRGQTIKLL KHRFSPNMTE REAANFIMKV IQNCFLSNRS RTYDMIQYYQ

NDIPY
Length:2,105
Mass (Da):237,042
Last modified:February 17, 2016 - v2
Checksum:i25EB66874330ED51
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140T8I9A0A140T8I9_MOUSE
Phosphatidylinositol 4-kinase alpha
Pi4ka
2,044Annotation score:
A0A338P6F9A0A338P6F9_MOUSE
Phosphatidylinositol 4-kinase alpha
Pi4ka
74Annotation score:
A0A338P7D3A0A338P7D3_MOUSE
Phosphatidylinositol 4-kinase alpha
Pi4ka
319Annotation score:
A0A338P6S0A0A338P6S0_MOUSE
Phosphatidylinositol 4-kinase alpha
Pi4ka
153Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti861P → L in AAH75629 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC110573 Genomic DNA No translation available.
BC049252 mRNA Translation: AAH49252.1
BC055479 mRNA Translation: AAH55479.1
BC075629 mRNA Translation: AAH75629.1
AK136942 mRNA Translation: BAE23184.1
RefSeqiNP_001001983.2, NM_001001983.2
XP_006522097.1, XM_006522034.1
UniGeneiMm.5718

Genome annotation databases

GeneIDi224020
KEGGimmu:224020
UCSCiuc007ykq.2 mouse
uc007ykr.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC110573 Genomic DNA No translation available.
BC049252 mRNA Translation: AAH49252.1
BC055479 mRNA Translation: AAH55479.1
BC075629 mRNA Translation: AAH75629.1
AK136942 mRNA Translation: BAE23184.1
RefSeqiNP_001001983.2, NM_001001983.2
XP_006522097.1, XM_006522034.1
UniGeneiMm.5718

3D structure databases

SMRiE9Q3L2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiE9Q3L2, 5 interactors
STRINGi10090.ENSMUSP00000036162

PTM databases

iPTMnetiE9Q3L2
PhosphoSitePlusiE9Q3L2

Proteomic databases

PaxDbiE9Q3L2
PeptideAtlasiE9Q3L2
PRIDEiE9Q3L2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi224020
KEGGimmu:224020
UCSCiuc007ykq.2 mouse
uc007ykr.2 mouse

Organism-specific databases

CTDi5297
MGIiMGI:2448506 Pi4ka

Phylogenomic databases

eggNOGiKOG0902 Eukaryota
COG5032 LUCA
HOGENOMiHOG000007272
HOVERGENiHBG103255
InParanoidiE9Q3L2
KOiK00888
OrthoDBiEOG091G03W5
TreeFamiTF102041

Miscellaneous databases

ChiTaRSiPi4ka mouse
PROiPR:E9Q3L2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041720 Expressed in 298 organ(s), highest expression level in bed nucleus of stria terminalis

Family and domain databases

Gene3Di1.10.1070.11, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR011009 Kinase-like_dom_sf
IPR000403 PI3/4_kinase_cat_dom
IPR036940 PI3/4_kinase_cat_sf
IPR018936 PI3/4_kinase_CS
IPR015433 PI_Kinase
IPR001263 PInositide-3_kin_accessory_dom
PANTHERiPTHR10048 PTHR10048, 1 hit
PfamiView protein in Pfam
PF00454 PI3_PI4_kinase, 1 hit
PF00613 PI3Ka, 1 hit
SMARTiView protein in SMART
SM00145 PI3Ka, 1 hit
SM00146 PI3Kc, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00915 PI3_4_KINASE_1, 1 hit
PS00916 PI3_4_KINASE_2, 1 hit
PS50290 PI3_4_KINASE_3, 1 hit
PS51545 PIK_HELICAL, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPI4KA_MOUSE
AccessioniPrimary (citable) accession number: E9Q3L2
Secondary accession number(s): Q3UVT5
, Q6DIC7, Q7TNG2, Q80Y45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: February 17, 2016
Last modified: November 7, 2018
This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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