Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Receptor-type tyrosine-protein phosphatase H

Gene

Ptprh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that may contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion-associated substrates and thus negatively regulating integrin-promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms: inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of BCAR1, focal adhesion kinase and SRC. Reduces migratory activity of Jurkat cells (By similarity). Reduces tyrosine phosphorylation of CEACAM20 and thereby contributes to suppress the intestinal immune response (PubMed:26195794).By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei871Phosphocysteine intermediatePROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase HImported (EC:3.1.3.481 Publication)
Short name:
R-PTP-HBy similarity
Alternative name(s):
Stomach cancer-associated protein tyrosine phosphatase 1By similarity
Short name:
SAP-1By similarity
Gene namesi
Name:PtprhImported
Synonyms:SAP-1Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:3026877 Ptprh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 604ExtracellularCuratedAdd BLAST577
Transmembranei605 – 625HelicalSequence analysisAdd BLAST21
Topological domaini626 – 971CytoplasmicCuratedAdd BLAST346

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Viable and fertile (PubMed:19170756). No significant effect on body weight or nutrient absorption (PubMed:19170756). Morphology of intestinal epithelium cells appears to be normal (PubMed:19170756). Double knockouts with APC heterozygotes, a model for intestinal tumorigenesis, show reduced adenoma growth (PubMed:19170756). Double knockouts with IL10, a model of inflammatory bowel disease, show significantly increased colonic inflammation in the presence of commensal bacteria and reduced survival rates (PubMed:26195794).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi837D → A: Impairs phosphatase activity. 1 Publication1
Mutagenesisi871C → S: Impairs phosphatase activity. 1 Publication1
Mutagenesisi945Y → S: Reduces tyrosine phosphorylation. Abolishes tyrosine phosphorylation and interaction with GRB2 and FYN; when associated with A-953. 1 Publication1
Mutagenesisi953Y → A: Reduces tyrosine phosphorylation. Abolishes tyrosine phosphorylation and interaction with GRB2 and FYN; when associated with A-945. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_500765467428 – 971Receptor-type tyrosine-protein phosphatase HAdd BLAST944

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi115N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi169N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi440N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi520N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei945Phosphotyrosine1 Publication1
Modified residuei953Phosphotyrosine1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiE9Q0N2
PRIDEiE9Q0N2

PTM databases

iPTMnetiE9Q0N2
PhosphoSitePlusiE9Q0N2

Expressioni

Tissue specificityi

Expressed strongly in the intestinal tract, with particularly high levels in the duodenum and jejunum (at protein level). Also expressed at low level in the testis (at protein level). Not detected in other tissues tested (at protein level).1 Publication

Developmental stagei

Detected from embryonic stage E16.5 onwards, with marked increase in expression level after birth.1 Publication

Gene expression databases

BgeeiENSMUSG00000035429 Expressed in 17 organ(s), highest expression level in colon

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Interacts with LCK (By similarity). Interacts (phosphorylated form) with GRB2 (via SH2 domain) (PubMed:20398064). Interacts (phosphorylated form) with FYN (via SH2 domain) (PubMed:20398064). Interacts (via extracellular domain) with CEACAM20 (via extracellular domain); the interaction dephosphorylates CEACAM20 (PubMed:26195794).By similarity2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000042396

Structurei

3D structure databases

ProteinModelPortaliE9Q0N2
SMRiE9Q0N2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 158Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST90
Domaini159 – 246Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST88
Domaini247 – 336Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST90
Domaini337 – 425Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST89
Domaini429 – 518Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST90
Domaini519 – 603Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST85
Domaini671 – 930Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST260

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0791 Eukaryota
COG5599 LUCA
GeneTreeiENSGT00900000140785
KOiK18034
OMAiTVWAERN
OrthoDBiEOG091G08FR
PhylomeDBiE9Q0N2
TreeFamiTF351926

Family and domain databases

CDDicd00063 FN3, 5 hits
Gene3Di2.60.40.10, 6 hits
3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR013783 Ig-like_fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR000242 PTPase_domain
IPR028855 R-PTP-H
IPR016130 Tyr_Pase_AS
IPR003595 Tyr_Pase_cat
IPR000387 TYR_PHOSPHATASE_dom
PANTHERiPTHR19134:SF297 PTHR19134:SF297, 3 hits
PfamiView protein in Pfam
PF00041 fn3, 5 hits
PF00102 Y_phosphatase, 1 hit
PRINTSiPR00700 PRTYPHPHTASE
SMARTiView protein in SMART
SM00060 FN3, 6 hits
SM00194 PTPc, 1 hit
SM00404 PTPc_motif, 1 hit
SUPFAMiSSF49265 SSF49265, 3 hits
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 6 hits
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50055 TYR_PHOSPHATASE_PTP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E9Q0N2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARAGGNCGV WRSLVLLGLY GCSVVRAAGT SVTVDRHAPA SSYEFSMWVE
60 70 80 90 100
KDGVSSSPQI PVTTAAPNPV RNLRVEGQNN ISISLSWEPP DQSSLQGLTY
110 120 130 140 150
WTQCSRHGGQ TETRNTTDTS VTVDGLDPGS SYECSVWVEK DGLYSKNETL
160 170 180 190 200
NTSTAPNPVR NLRVEGQNNI SISLSWEPPD QPSLQGLTYW AQCSRHGGQT
210 220 230 240 250
ETRNTADTSV TVDGLDPGSS YECSVWVEKD GVYSTNETLN TSTAPNPVRN
260 270 280 290 300
LRVEGQNNIS ISLSWEPPDQ PSLQGLTYWA QCSRHGGQTE TRNTTDTSIT
310 320 330 340 350
VDGLDPGSSY ECSVWVEKDG VYSTNETLSN TTAPNPVRNL RVKSQNNFSI
360 370 380 390 400
SLSWEPPDQP SLQGLIYWAQ CSRHGGQTET RNTTDTSVTV DGLDPGFLYK
410 420 430 440 450
CSVWVEKDGV YSTNETLNTS TVPISASNPV RNLRVEGQNN FSISLSWEPP
460 470 480 490 500
DQSSLQGLTY WAQCSRHGGQ TETRNTADTS VTVDGLDPGS SYECSVWVEK
510 520 530 540 550
DGVYSTNETL NTSTVPAAVN ITSCISTSGG YGVLLTWSCP SGGYESFEVK
560 570 580 590 600
VGRKWRSENG SLCGKGVTVS DLEPAQSYTA TVTTVFKDLK AQSLSTTCHT
610 620 630 640 650
ESAAIIAGAI VGILLLFILV GLLIVFLKRR RKKRQPKEVP KDLVCSCPGD
660 670 680 690 700
ILAKDFADHV RENEKDSNCG FAEEYQQLAL EGQGQSQITA SALENRSKNR
710 720 730 740 750
YRNVLPYDWS RVPLQPLQEE PGSDYINASF MPGLWSPKEF IATQGPLPNT
760 770 780 790 800
VGDFWRMVWE QQSHTLVMLT NCMESGRVKC EHYWPLDAQP CIHGQLQVML
810 820 830 840 850
ISEEASENWT VRHLQLFHMK EQQTLSLRQF HYLAWPDHGV PYSPDPLLAF
860 870 880 890 900
RKMLRQWMDQ TTDGGPPIVH CSAGVGRTGT LIALDVLLRQ LECEGLVGPF
910 920 930 940 950
SFVKKMRESR PLMVQTEAQY VFLHQCILKS LQKPAPALVP EEAMYENVAS
960 970
LVYENASAIM AHESEFSASG C
Length:971
Mass (Da):106,952
Last modified:April 5, 2011 - v1
Checksum:i9992614E4A5C1543
GO

Sequence cautioni

The sequence BAC37443 differs from that shown. Reason: Frameshift at positions 228, 298 and 377.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182P → S in BAH03209 (PubMed:19170756).Curated1
Sequence conflicti436 – 437EG → KS in BAH03209 (PubMed:19170756).Curated2
Sequence conflicti453S → P in BAH03209 (PubMed:19170756).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB217856 mRNA Translation: BAH03209.1
AK078884 mRNA Translation: BAC37443.1 Frameshift.
AC161197 Genomic DNA No translation available.
AC161218 Genomic DNA No translation available.
CCDSiCCDS51974.1
RefSeqiNP_997153.2, NM_207270.2
UniGeneiMm.188925

Genome annotation databases

EnsembliENSMUST00000049113; ENSMUSP00000042396; ENSMUSG00000035429
ENSMUST00000166650; ENSMUSP00000125833; ENSMUSG00000035429
ENSMUST00000206999; ENSMUSP00000145543; ENSMUSG00000035429
GeneIDi545902
KEGGimmu:545902
UCSCiuc009exz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPTPRH_MOUSE
AccessioniPrimary (citable) accession number: E9Q0N2
Secondary accession number(s): B6ZDS3, Q8BIW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2016
Last sequence update: April 5, 2011
Last modified: September 12, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again